KEGG   ENZYME: 4.1.2.10
Entry
EC 4.1.2.10                 Enzyme                                 
Name
(R)-mandelonitrile lyase;
(R)-oxynitrilase;
oxynitrilase;
D-oxynitrilase;
D-alpha-hydroxynitrile lyase;
mandelonitrile benzaldehyde-lyase;
PaHNL;
AtHNL;
PhaMDL;
(R)-HNL;
(R)-PeHNL;
(R)-hydroxynitrile lyase;
R-selective hydroxynitrile lyase;
R-selective HNL;
(R)-(+)-mandelonitrile lyase
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
Sysname
(R)-mandelonitrile benzaldehyde-lyase (cyanide-forming)
Reaction(IUBMB)
(R)-mandelonitrile = cyanide + benzaldehyde [RN:R01767]
Reaction(KEGG)
R01767;
(other) R01409 R02811
Substrate
(R)-mandelonitrile [CPD:C00561]
Product
cyanide [CPD:C00177];
benzaldehyde [CPD:C00261]
Comment
A variety of enzymes from different sources and with different properties. Some are flavoproteins, others are not. Active towards a number of aromatic and aliphatic hydroxynitriles (cyanohydrins).
History
EC 4.1.2.10 created 1961, modified 1999, modified 2011
Pathway
ec00460  Cyanoamino acid metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K08248  (R)-mandelonitrile lyase
K20802  (R)-mandelonitrile lyase
Genes
ATH: AT1G73050 AT5G10300(MES5)
ALY: 9309518 9324968
CRB: 17882602 17894548
CSAT: 104701992 104708070 104712914 104735043 104751159 104769234
EUS: EUTSA_v10014451mg EUTSA_v10018360mg
BRP: 103831793 103846819 103852822
BNA: 106354249 106372481 106381275 106402693 106404872
BOE: 106299217 106312968 106327722
RSZ: 108814498 108841961 108841964
THJ: 104798892 104812207
CPAP: 110824699 110824820
CIT: 102626291
CIC: CICLE_v10025302mg
PVY: 116104932
MINC: 123212454 123212483
TCC: 18595196
GRA: 105767552
GHI: 107906346 107958501
GAB: 108453003 108465479
HSYR: 120133426 120148998 120191477
DZI: 111280688
EGR: 104423258
RARG: 115755855
GMX: 100820069
GSJ: 114419167
PVU: 137819648
VRA: 106773780
VAR: 108320669
VUN: 114165905
VUM: 124835873
CCAJ: 109808261
APRC: 113857241
MTR: 11434866
TPRA: 123923187
CAM: 101508164
PSAT: 127107124
VVO: 131606069
LJA: 130745669
ADU: 107482361
AIP: 107635832
AHF: 112740806 112795530
LANG: 109356592
PCIN: 129287440 129291371 129295005
QSA: O6P43_017898 O6P43_027981
FVE: 101298340 101314080
AANS: 126790237 126791524 126799605
RCN: 112189159 112195896 112199010 112200405
PPER: 109946273 109946518 109946533 109946707 109946708 109946737 109948219 109948555 18788267 18788307 18789360 18790170 18790921 18792725 18792970
PMUM: 103323319 103323417 103323418 103323458 103323460 103323461 103323462 103323463 103323464 103323465 103323466 103328816 103344346 103344354
PAVI: 110748378 110749710 110752511 110752512 110752514 110752517 110752520 110752523 110752528 110752533 110752546 110767481 110772812 110774301
PDUL: 117614487 117614647 117614649 117615086 117615220 117615239 117615281 117615325 117615505 117617267 117623911 117635510
MDM: 103404510 103416927 103417232 103417299 103420796 103429895 103429959 103443315 103447443 103447445 103447446 114819207 114823910
MSYL: 126583382 126591179 126591180 126591181 126591182 126595139 126606559 126607412 126610129 126616874 126616875 126616876
PXB: 103926833 103926855 103931741 103944089 103944905 103944906 103944908 103954303 108867073
ZJU: 107405884 107405886 107405923 107405924 107405925 107405927 107407673 107410829 107410921 107411003 107414212 107414223 107414239 107414267 107414317 107414349 107414388 107415173 107428185 107432121 107432123 107432124 112493444 125418345 125423758 125424306 132799199 132800150 132800219 132800225 132800258 132800283 132800336 132800337 132800343 132800347 132800821 132803410 132805279 132805365 132805367 132805371
MNT: 21388472 21405661 21405817 21405818 21405819
CSAV: 115694894 115694897 115695176 115695919 115695926 115695941 115714652 115718612 115724435 115724498 115724686 115725081 115725720
CSV: 101211615 101212821 101213062 101213549 101213785 101214030 101214272 101214723 101218493 116401960 116401973 116401986
CMO: 103493044 103493053 103493064 103493073 103493083 103493093 103493130 103493164 103493179 103493189 103500635
BHJ: 120079226 120079227 120079228 120079550 120080282 120080549 120086734
MCHA: 111020834 111020835 111020836 111020841
CMAX: 111465496 111465498 111488606 111492330 111492348 111494473
CMOS: 111436113 111436125 111436223 111444087 111444097 111450816 111456379 111464460
CPEP: 111779136 111799103 111804863 111805062 111805571 111805875 111807599
RCU: 107260750
MEAN: 126678143
JCU: 105643749
HBR: 110655857
MESC: 110603558 110628615
POP: 7490862
PEU: 105131644 105141162
PALZ: 118045144 118059813
PNZ: 133704143
JRE: 108983056 109004998
CILL: 122300687 122300854
CAVE: 132187742
AGLU: 133864858
QSU: 112001779
QLO: 115989370
TWL: 120008047 120011473
VVI: 100254058
VRI: 117905746
SLY: 101250204
SPEN: 107014093
SOT: 102580581
SSTN: 125857664
SDUL: 129896937
CANN: 107865788
LBB: 132634393
NTA: 107771021 107780107
NSY: 104246964
NTO: 104107041
NAU: 109224695
INI: 109187446 109187563 109192240
ITR: 115996468 116011297
SIND: 105157899
OEU: 111401494
EGT: 105954354
SSPL: 121778127 121781122
SMIL: 131013069
SHIS: 125187800 125208472
APAN: 127241769
HAN: 110874223 110877473 110877524 110877525 110886397 110886885 110886886 110894152 110913082 110916839 110917615 110929299 110929300 110929307 110929308 110931731
ECAD: 122589929 122597922 122599479 122600961
LSV: 111879508 111909027 111910381 111915295
CCAV: 112504716 112509255 112517338
DCR: 108225454 108227163
CSIN: 114311932
RVL: 131335570
AEW: 130796241
DLT: 127789797 127794850 127805653 127805730 127806139 127806140 127806142 127806143 127806144 127806145 127806146
BVG: 104901404
SOE: 110785208
CQI: 110706134 110713236
ATRI: 130824369
MOF: 131157848 131159594 131159597 131159600
NNU: 104599090
MING: 122073004
TSS: 122657587
PSOM: 113275657 113277545
OSA: 9266710
DOSA: Os06g0656000
OBR: 102719124
OGL: 127775358
BDI: 100822804
ATS: 109783110
TDC: 119333926 119339393
TAES: 123154811 123160447 123171371
TUA: 125525090
LPER: 127311746
LRD: 124684348 124684531
SBI: 8069366
ZMA: 100382914
MFLO: 136522833 136523076 136525190
SITA: 101763110
SVS: 117853312
PVIR: 120671531 120704924
PHAI: 112888364
PAUA: 133927763
PDA: 103715972
EGU: 105039402
MUS: 135606714
DCT: 110096808
AOF: 109842577 109842578 109842616
MSIN: 131256359 131256384
ACAK: 137950614
NCOL: 116250416
ATR: 18438828 18440898
CJF: 131077178
 » show all
Reference
1  [PMID:18540101]
  Authors
Ueatrongchit T, Kayo A, Komeda H, Asano Y, H-Kittikun A
  Title
Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (Loquat).
  Journal
Biosci Biotechnol Biochem 72:1513-22 (2008)
DOI:10.1271/bbb.80023
Reference
2
  Authors
Lin G, Han S, Li Z.
  Title
Enzymic synthesis of (R)-cyanohydrins by three (R)-oxynitrilase sources in micro-aqueous organic medium.
  Journal
Tetrahedron 55:3531-3540 (1999)
Reference
3
  Authors
de Gonzalo G, Brieva R, Gotor V.
  Title
(R)-Oxynitrilase-catalyzed transformation of omega-hydroxyalkanals.
  Journal
J Mol Catal B 19-20:223-230 (2002)
Reference
4  [PMID:25919621]
  Authors
Ueatrongchit T, Tamura K, Ohmiya T, H-Kittikun A, Asano Y
  Title
Hydroxynitrile lyase from Passiflora edulis: Purification, characteristics and application in asymmetric synthesis of (R)-mandelonitrile.
  Journal
Enzyme Microb Technol 46:456-65 (2010)
DOI:10.1016/j.enzmictec.2010.02.008
Reference
5  [PMID:17907254]
  Authors
Andexer J, von Langermann J, Mell A, Bocola M, Kragl U, Eggert T, Pohl M
  Title
An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an alpha/beta-hydrolase fold.
  Journal
Angew Chem Int Ed Engl 46:8679-81 (2007)
DOI:10.1002/anie.200701455
  Sequence
[ath:AT5G10300]
Reference
6  [PMID:19433222]
  Authors
Guterl JK, Andexer JN, Sehl T, von Langermann J, Frindi-Wosch I, Rosenkranz T, Fitter J, Gruber K, Kragl U, Eggert T, Pohl M
  Title
Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold.
  Journal
J Biotechnol 141:166-73 (2009)
DOI:10.1016/j.jbiotec.2009.03.010
  Sequence
[ath:AT5G10300]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.10
IUBMB Enzyme Nomenclature: 4.1.2.10
ExPASy - ENZYME nomenclature database: 4.1.2.10
BRENDA, the Enzyme Database: 4.1.2.10
CAS: 9024-43-5

  All links  
Pathway (6)   
   KEGG PATHWAY (6)   
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (7)   
   KEGG REACTION (3)   
   KEGG RCLASS (4)   
Gene (437)   
   KEGG ORTHOLOGY (2)   
   KEGG GENES (434)   
   RefGene (1)   
Protein sequence (438)   
   UniProt (391)   
   SWISS-PROT (9)   
   RefSeq(pep) (6)   
   PDBSTR (32)   
DNA sequence (66)   
   RefSeq(nuc) (11)   
   GenBank (39)   
   EMBL (16)   
3D Structure (21)   
   PDB (21)   
Protein domain (14)   
   InterPro (13)   
   PROSITE(DOC) (1)   
All databases (996)   

Download RDF
DBGET integrated database retrieval system