| Entry |
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| Name |
4-hydroxy-2-oxoheptanedioate aldolase;
2,4-dihydroxyhept-2-enedioate aldolase;
HHED aldolase;
4-hydroxy-2-ketoheptanedioate aldolase;
HKHD aldolase;
HpcH;
HpaI;
4-hydroxy-2-oxoheptanedioate succinate semialdehyde lyase (pyruvate-forming)
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| Class |
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
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| Sysname |
4-hydroxy-2-oxoheptanedioate succinate-semialdehyde-lyase (pyruvate-forming)
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| Reaction(IUBMB) |
4-hydroxy-2-oxoheptanedioate = pyruvate + succinate semialdehyde [RN: R01645]
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| Reaction(KEGG) |
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| Substrate |
4-hydroxy-2-oxoheptanedioate [CPD: C05601]
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| Product |
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| Comment |
Requires Co2+ or Mn2+ for activity. The enzyme is also able to catalyse the aldol cleavage of 4-hydroxy-2-oxopentanoate and 4-hydroxy-2-oxohexanoate, and can use 2-oxobutanoate as carbonyl donor, with lower efficiency. In the reverse direction, is able to condense a range of aldehyde acceptors with pyruvate. The enzyme from the bacterium Escherichia coli produces a racemic mixture of (4R)- and (4S)-hydroxy-2-oxoheptanedioate [4].
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| History |
EC 4.1.2.52 created 2013
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| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K02510 | 4-hydroxy-2-oxoheptanedioate aldolase |
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| Genes |
» show all
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| Reference |
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| Authors |
Wang W, Seah SY |
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| Title |
Purification and biochemical characterization of a pyruvate-specific class II aldolase, HpaI. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Rea D, Fulop V, Bugg TD, Roper DI |
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| Title |
Structure and mechanism of HpcH: a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Wang W, Seah SY |
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| Title |
The role of a conserved histidine residue in a pyruvate-specific Class II aldolase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Wang W, Baker P, Seah SY |
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| Title |
Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 4.1.2.52 |
| ExPASy - ENZYME nomenclature database: | 4.1.2.52 |
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