KEGG   ENZYME: 4.1.2.55
Entry
EC 4.1.2.55                 Enzyme                                 

Name
2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase;
2-keto-3-deoxygluconate aldolase (ambiguous);
KDGA (ambiguous)
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
Sysname
2-dehydro-3-deoxy-6-phospho-D-gluconate/2-dehydro-3-deoxy-6-phospho-D-galactonate D-glyceraldehyde-3-phosphate-lyase (pyruvate-forming)
Reaction(IUBMB)
(1) 2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate [RN:R05605];
(2) 2-dehydro-3-deoxy-6-phospho-D-galactonate = pyruvate + D-glyceraldehyde 3-phosphate [RN:R01064]
Reaction(KEGG)
R01064 R05605;
(other) R08570 R10616
Substrate
2-dehydro-3-deoxy-6-phospho-D-gluconate [CPD:C04442];
2-dehydro-3-deoxy-6-phospho-D-galactonate [CPD:C01286]
Product
pyruvate [CPD:C00022];
D-glyceraldehyde 3-phosphate [CPD:C00118]
Comment
In the archaeon Sulfolobus solfataricus the enzyme is involved in glucose and galactose catabolism via the branched variant of the Entner-Doudoroff pathway. It utilizes 2-dehydro-3-deoxy-6-phosphate-D-gluconate and 2-dehydro-3-deoxy-6-phosphate-D-galactonate with similar catalytic efficiency. In vitro the enzyme can also catalyse the cleavage of the non-phosphorylated forms 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate with much lower catalytic efficiency. cf. EC 4.1.2.21, 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, and EC 4.1.2.14, 2-dehydro-3-deoxy-phosphogluconate aldolase.
History
EC 4.1.2.55 created 2014
Pathway
ec00030  Pentose phosphate pathway
ec00052  Galactose metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K11395  2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
Genes
VBS: EGM51_13350
TAC: Ta0619
TVO: TVG0663048(TVG0663048)
PTO: PTO1026
FAC: FACI_IFERC01G1727
FAI: FAD_0700
HAL: VNG_0444G(dapA)
HSL: OE_1665R(kdgA)
HAH: Halar_3720
STO: STK_24790(kdgA)
SSO: SSO3197(eda)
SOL: Ssol_0934
SSOA: SULA_0967
SSOL: SULB_0969
SSOF: SULC_0968
SAI: Saci_0225
SID: M164_2166
SII: LD85_2429
SIH: SiH_2108
SIR: SiRe_2040
SIC: SiL_2015
MSE: Msed_1296
MCN: Mcup_0949
AHO: Ahos_0260
STEP: IC006_2133
PAS: Pars_1388
PYR: P186_1933
POG: Pogu_0825
CMA: Cmaq_0935
TTN: TTX_1156a(kdgA)
TUZ: TUZN_0957
VDI: Vdis_1187
VMO: VMUT_2035
 » show all
Reference
1  [PMID:10527934]
  Authors
Buchanan CL, Connaris H, Danson MJ, Reeve CD, Hough DW
  Title
An extremely thermostable aldolase from Sulfolobus solfataricus with specificity  for non-phosphorylated substrates.
  Journal
Biochem J 343 Pt 3:563-70 (1999)
DOI:10.1042/bj3430563
  Sequence
Reference
2  [PMID:16330030]
  Authors
Lamble HJ, Theodossis A, Milburn CC, Taylor GL, Bull SD, Hough DW, Danson MJ
  Title
Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon  Sulfolobus solfataricus.
  Journal
FEBS Lett 579:6865-9 (2005)
DOI:10.1016/j.febslet.2005.11.028
  Sequence
Reference
3  [PMID:17176250]
  Authors
Wolterink-van Loo S, van Eerde A, Siemerink MA, Akerboom J, Dijkstra BW, van der Oost J
  Title
Biochemical and structural exploration of the catalytic capacity of Sulfolobus KDG aldolases.
  Journal
Biochem J 403:421-30 (2007)
DOI:10.1042/BJ20061419
  Sequence
[sso:SSO3197] [sai:Saci_0225] [sto:STK_24790]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.55
IUBMB Enzyme Nomenclature: 4.1.2.55
ExPASy - ENZYME nomenclature database: 4.1.2.55
BRENDA, the Enzyme Database: 4.1.2.55

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