Entry
Name
malyl-CoA lyase;
malyl-coenzyme A lyase;
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase;
mclA (gene name);
mcl1 (gene name);
(3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase (acetyl-CoA-forming);
L-malyl-CoA lyase
Class
Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
BRITE hierarchy
Sysname
(S)-malyl-CoA glyoxylate-lyase (acetyl-CoA-forming)
Reaction(IUBMB)
(1) (S)-malyl-CoA = acetyl-CoA + glyoxylate [RN:
R00473 ];
(2) (2R,3S)-2-methylmalyl-CoA = propanoyl-CoA + glyoxylate [RN:
R00934 ]
Reaction(KEGG)
Substrate
(S)-malyl-CoA [CPD:
C04348 ];
(2R,3S)-2-methylmalyl-CoA [CPD:
C06027 ]
Product
Comment
The enzymes from Rhodobacter species catalyse a step in the ethylmalonyl-CoA pathway for acetate assimilation [3,5]. The enzyme from halophilic bacteria participate in the methylaspartate cycle and catalyse the reaction in the direction of malyl-CoA formation [6]. The enzyme from the bacterium Chloroflexus aurantiacus, which participates in the 3-hydroxypropanoate cycle for carbon assimilation, also has the activity of EC
4.1.3.25 , (3S)-citramalyl-CoA lyase [2,4].
History
EC 4.1.3.24 created 1972, modified 2014
Pathway
ec00630 Glyoxylate and dicarboxylate metabolism
ec00660 C5-Branched dibasic acid metabolism
ec00720 Other carbon fixation pathways
ec01120 Microbial metabolism in diverse environments
Orthology
K08691 malyl-CoA/(S)-citramalyl-CoA lyase
K19281 beta-methylmalyl-CoA/(S)-malyl-CoA lyase
K19282 bifunctional (S)-malyl-CoA lyase/thioesterase
Genes
LLG : 44548918_02118(mcl1)
CBLY : IPM89_03270 IPM89_04980
PSAA : QEN71_12140 QEN71_33975
URU : DSM104443_00577(mcl1_1)
RBS : RHODOSMS8_01011(mcl)
SFH : SFHH103_02474(cite1)
SFD : USDA257_c22030(mcl1)
SAME : SAMCFNEI73_pC0656(mcl)
MEA : Mex_1p1733(mcl) Mex_1p4295
MDI : METDI2485(mcl) METDI4906
MZA : B2G69_01480 B2G69_16670
MRD : Mrad2831_1650 Mrad2831_3663
META : Y590_08615 Y590_19480
MAQU : Maq22A_c18385(citE) Maq22A_c19250(mcl)
MPHY : MCBMB27_01298 MCBMB27_03145
MEE : DA075_20140 DA075_27160
METD : C0214_10380 C0214_21595
METX : A3862_13170 A3862_22625
METS : DK389_15360 DK389_27325
METI : DK427_15555 DK427_17480
MMES : MMSR116_01155 MMSR116_21315
MTEA : DK419_14290 DK419_26925
MIND : mvi_25720 mvi_28860
MOG : MMB17_01150 MMB17_19730
MTAD : M6G65_09600 M6G65_20420
MECL : CLZ_08145 CLZ_19310
MTUN : MTUNDRAET4_3402(mcl)
HDT : HYPDE_30183 HYPDE_38943
HMC : HYPMC_1941 HYPMC_4395(mcl)
HNI : W911_10365 W911_15805
FIL : BN1229_v1_1240(mcl) BN1229_v1_2672
FIY : BN1229_v1_1238(mcl) BN1229_v1_3242
MBRY : B1812_00060 B1812_17545
MROS : EHO51_12185 EHO51_15075
MHEY : H2LOC_011805 H2LOC_014625
MPAR : F7D14_06360 F7D14_09175
MIWA : SS37A_01470 SS37A_07190
MTW : CQW49_12235 CQW49_16215
MECQ : MSC49_04610 MSC49_35030(mclA)
CMET : K6K41_15080 K6K41_22170
PLEO : OHA_1_00681(mcl) OHA_1_00777(citE)
HDI : HDIA_2217(mcl1_1) HDIA_2467(mcl1_2)
TSV : DSM104635_00778(mcl1)
SIL : SPO1565 SPO3608(mclA)
RUA : D1823_18170 D1823_20490
RUT : FIU92_13270(mcl2) FIU92_19865(mcl3)
RCON : K3740_00765 K3740_20040
RUY : NOR97_12580 NOR97_17625
RDE : RD1_1001(citE) RD1_1160(mclA) RD1_4242(mclA)
RLI : RLO149_c002070(mcl1) RLO149_c036850(mcl2)
RFU : ROLI_001060(mcl1_1) ROLI_003020(mcl1_2)
DSH : Dshi_2490(citE1) Dshi_3050(citE2)
LMD : METH_02340 METH_18390
LEJ : ETW24_03080 ETW24_17910
LAQU : R2C4_14570 R2C4_19790
LEV : ETW23_00250 ETW23_17075
LCAE : K3721_02490 K3721_16185
SINL : DSM14862_03056(mcl1)
SPSE : SULPSESMR1_03496(mcl1)
RMM : ROSMUCSMR3_02149(mcl1) ROSMUCSMR3_03455(mcl1)
ROK : RAK1035_1037 RAK1035_2340
RID : RIdsm_02779(mcl1_1) RIdsm_05011(mcl1_2)
ROM : EI983_00710 EI983_08270
RPEL : N7U68_03480 N7U68_09695
AHT : ANTHELSMS3_00280(mcl1)
SAGU : CDO87_13335 CDO87_15505
SSTL : ABFK29_04820 ABFK29_06700
MALU : KU6B_08980(mcl1) KU6B_14460 KU6B_40050
PALX : GQA70_15345 GQA70_23220
RMAI : MACH21_17090 MACH21_30420(mcl1)
RED : roselon_01454 roselon_02260
FYT : QF092_14495 QF092_14520
PAMI : JCM7686_2763 JCM7686_3168
PZH : CX676_04845 CX676_15565
PAMN : JCM7685_2683 pAMV1p0135(mcl) pAMV3p0611(mcal)
PSAP : JHX88_01975 JHX88_17895 JHX88_20315
PALP : JHW40_15150 JHW40_18530
LVS : LOKVESSMR4R_02838(mcl1)
GEH : HYN69_16130 HYN69_16155
GFU : KM031_08955 KM031_11830
TAW : EI545_00080 EI545_09215
SEDI : EBB79_18450 EBB79_24155
PAED : G5B38_01665 G5B38_14245
NSM : JO391_00315 JO391_12675
AMYL : QBD29_03580 QBD29_08415
AMAQ : GO499_03725 GO499_18685
RBG : BG454_02060 BG454_05615
BOSG : GKR98_07030 GKR98_16035
RCP : RCAP_rcc03164(citE2)
HADH : FRZ61_42590 FRZ61_45950
SNIG : HEK616_29400(citE_1)
NAQU : ENKNEFLB_04247(mcl)
BALA : DSM104299_01895(mcl)
SBAE : DSM104329_01824(mcl) DSM104329_03745(mcl1)
HALX : M0R89_18090 M0R89_21245
HMA : rrnAC0690(citE1) rrnAC1965(aceB)
HHI : HAH_1341(citE1) HAH_2476(aceB)
HHN : HISP_06865 HISP_12605
HAB : SG26_04910 SG26_14710
HTA : BVU17_06535 BVU17_12045
HSIN : KDQ40_00995 KDQ40_06765
HVO : HVO_1983(aceB1) HVO_B0200(aceB2)
HALE : G3A49_07690 G3A49_12900
HLN : SVXHx_2637(aceb) SVXHx_3054(aceb)
HGI : ABY42_09665 ABY42_16600
HSAL : JMJ58_00685 JMJ58_24075
NMG : Nmag_3338(mcl) Nmag_3363(aceB)
NOU : Natoc_1509 Natoc_4308
NAJ : B1756_06555 B1756_12025
NBG : DV706_12980 DV706_17910
NAS : GCU68_13725 GCU68_18820
» show all
Taxonomy
Reference
Authors
TUBOI S, KIKUCHI G.
Title
ENZYMIC CLEAVAGE OF MALYTL-COENZYME A INTO ACETYL-COENZYME A AND GLYOXYLIC ACID.
Journal
Reference
Authors
Herter S, Busch A, Fuchs G
Title
L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation.
Journal
Sequence
Reference
Authors
Meister M, Saum S, Alber BE, Fuchs G
Title
L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus.
Journal
Sequence
Reference
Authors
Friedmann S, Alber BE, Fuchs G
Title
Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus.
Journal
Reference
Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase.
Journal
Sequence
Reference
Authors
Borjian F, Han J, Hou J, Xiang H, Zarzycki J, Berg IA.
Title
Malate Synthase and beta-Methylmalyl Coenzyme A Lyase Reactions in the Methylaspartate Cycle in Haloarcula hispanica.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 4.1.3.24
ExPASy - ENZYME nomenclature database: 4.1.3.24