Entry
Name
tryptophanase;
L-tryptophanase;
L-tryptophan indole-lyase (deaminating);
TNase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
L-tryptophan indole-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-tryptophan + H2O = indole + pyruvate + NH3 (overall reaction) [RN:
R00673 ];
(1a) L-tryptophan = indole + 2-aminoprop-2-enoate [RN:
R12758 ];
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous) [RN:
R11100 ];
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous) [RN:
R11099 ]
Reaction(KEGG)
Substrate
Product
Comment
A pyridoxal-phosphate protein, requiring K+. The enzyme cleaves a carbon-carbon bond, releasing indole and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC
3.5.99.10 , 2-iminobutanoate/2-iminopropanoate deaminase. Also catalyses 2,3-elimination and beta-replacement reactions of some indole-substituted tryptophan analogues of L-cysteine, L-serine and other 3-substituted amino acids.
History
EC 4.1.99.1 created 1972
Pathway
Orthology
Genes
MAW : 19252427(J3458_019562)
EHI : EHI_111610(347.t00005)
ECOO : ECRM13514_4774(tnaA)
ECOH : ECRM13516_4558(tnaA)
ELR : ECO55CA74_21605(tnaA)
ECW : EcE24377A_4217(tnaA)
EDJ : ECDH1ME8569_3596(tnaA)
ECOI : ECOPMV1_04046(tnaA)
KLE : AO703_10280(tnaA) AO703_20280(tnaA)
AHN : NCTC12129_03675(tnaA)
METY : MRY16398_49300(tnaA)
PSHI : SAMEA2665130_0893(tnaA)
SOF : NCTC11214_00995(tnaA)
SERA : Ser39006_006065(tnaA)
DIC : Dpoa569_000581(tnaA)
XNE : XNC1_0444(tnaA) XNC1_2962(tnaA)
XNM : XNC2_0435(tnaA) XNC2_2841(tnaA)
XHO : A9255_12890 A9255_13910(tnaA)
PHEI : NCTC12003_03267(tnaA)
PRJ : NCTC6933_03369(tnaA)
HIC : NTHIC486_00286(tnaA)
HHZ : NCTC10839_00483(tnaA)
PDAG : 4362423_01740(tnaA)
RPNE : NCTC8284_00193(tnaA_1)
VCE : Vch1786_II0954(tnaA)
VOW : A9237_17005(tnaA) A9237_24140
VAU : VANGNB10_cI0734c(tnaA)
VCY : IX92_23840(tnaA) IX92_27170(tnaA)
VCT : JV59_24025(tnaA) JV59_25700(tnaA) JV59_30555(tnaA)
VTU : IX91_08130(tnaA) IX91_22075(tnaA) IX91_23675(tnaA)
VEU : IXK98_05775 IXK98_16740(tnaA) IXK98_24560
VSC : VSVS12_04542 VSVS12_04570
VSH : BSZ05_15630 BSZ05_23090(tnaA)
VPL : SA104470976_03032(tnaA)
VNP : KW548_09630(tnaA) KW548_19730
VIB : VspSTUT11_27920(tnaA)
VIS : VspSTUT16_41200(tnaA)
PPR : PBPRA1344 PBPRA2532 PBPRB0382
PGH : FH974_23495(tnaA) FH974_23590
AEL : NCTC12917_04171(tnaA)
CHJ : NCTC10426_01845(tnaA)
VFA : MM35RIKEN_12190(tpl)
ACOL : K5I23_08455 K5I23_12190
INT : RX717_07130 RX717_14280
STH : STH439(tna-2) STH441(tna-1)
DDH : Desde_1258 Desde_1942
CBAR : PATL70BA_2536(tnaA)
SVL : Strvi_6349 Strvi_8511
SPHV : F9278_00830 F9278_05175
SCHA : CP983_30385 CP983_43305
STUI : GCM10017668_67990(tnaA)
SDEC : L3078_01570 L3078_06625
SAKB : K1J60_38775 K1J60_43840
SCAE : IHE65_38895 IHE65_44005
PAV : TIA2EST22_05315(tnaA)
PAX : TIA2EST36_05285(tnaA)
PAZ : TIA2EST2_05225(tnaA)
PCN : TIB1ST10_05480(tnaA)
PACN : TIA1EST1_05245(tnaA)
AMYC : CU254_00135 CU254_12745 CU254_42175
AACD : LWP59_01735 LWP59_22600
ACTU : Actkin_02732(tnaA1)
FPOL : ERS445057_02251(tnaA1)
BTHO : Btheta7330_01722(tpl)
BOA : Bovatus_01573(tnaA1)
BCEL : BcellWH2_01052(tpl)
PCRE : NCTC12858_01229(tpl_2)
PCAG : NCTC12856_01907(tnaA2)
ADA : A5CPEGH6_16250(tnaA)
CSAL : NBC122_00880(tpl_1)
CHRJ : CHRYMOREF3P_2738(tnaA)
» show all
Taxonomy
Reference
Authors
BURNS RO, DEMOSS RD.
Title
Properties of tryptophanase from Escherichia coli.
Journal
Reference
Authors
NEWTON WA, MORINO Y, SNELL EE.
Title
PROPERTIES OF CRYSTALLINE TRYPTOPHANASE.
Journal
J Biol Chem 240:1211-8 (1965)
Reference
3
Authors
Cowell JL, Maser K, DeMoss RD.
Title
Tryptophanase from Aeromonas liquifaciens. Purification, molecular weight and some chemical, catalytic and immunological properties.
Journal
Biochim Biophys Acta 315:449-463 (1973)
Reference
Authors
Snell EE
Title
Tryptophanase: structure, catalytic activities, and mechanism of action.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.1
ExPASy - ENZYME nomenclature database: 4.1.99.1