Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases

D-ribulose 5-phosphate formate-lyase (L-3,4-dihydroxybutan-2-one 4-phosphate-forming)

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate [RN:R07281]

R07281

D-ribulose 5-phosphate [CPD:C00199]

Requires a divalent cation, preferably Mg2+, for activity [1]. The reaction involves an intramolecular skeletal rearrangement, with the bonds in D-ribulose 5-phosphate that connect C-3 and C-5 to C-4 being broken, C-4 being removed as formate and reconnection of C-3 and C-5 [1]. The phosphorylated four-carbon product (L-3,4-dihydroxybutan-2-one 4-phosphate) is an intermediate in the biosynthesis of riboflavin [1].

EC 4.1.99.12 created 2007