Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
spore photoproduct pyrimidine-lyase
Reaction(IUBMB)
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA) = thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Substrate
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA)
Product
thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Comment
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. The enzyme binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule [3]. The 5'-deoxy-adenosine radical formed after electron transfer from the [4Fe-4S] cluster to the S-adenosyl-L-methionine, initiates the repair by abstracting the C-6 hydrogen of the spore photoproduct lesion. During the second part of the repair process the SAM molecule is regenerated [3].
Pieck JC, Hennecke U, Pierik AJ, Friedel MG, Carell T
Title
Characterization of a new thermophilic spore photoproduct lyase from Geobacillus stearothermophilus (SplG) with defined lesion containing DNA substrates.