Entry
Name
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
germacradienol geosmin-lyase (acetone-forming)
Reaction(IUBMB)
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O = (-)-geosmin + acetone [RN:
R09487 ]
Reaction(KEGG)
Substrate
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol [CPD:
C16143 ];
H2O [CPD:
C00001 ]
Product
Comment
Requires Mg2+. Geosmin is the cause of the characteristic smell of moist soil. It is a bifunctional enzyme. The N-terminal part of the enzyme is EC
4.2.3.22 , germacradienol synthase, and forms germacradienol from FPP. The C-terminal part of the enzyme catalyses the conversion of germacradienol to geosmin via (1S,4aS,8aS)-1,4a-dimethyl-1,2,3,4,4a,5,6,8a-octahydronaphthalene.
History
EC 4.1.99.16 created 2011
Pathway
ec00909 Sesquiterpenoid and triterpenoid biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K10187 germacradienol/geosmin synthase
Genes
AGE : AA314_02305 AA314_08732
NANN : O0S08_24410 O0S08_35550
SLE : sle_16070(sle_16070)
SALW : CP975_28380 CP975_33095
SGM : GCM10017557_17550(cyc2)
STUI : GCM10017668_55740(cyc2)
SGOB : test1122_08905 test1122_25330
SXT : KPP03845_105794(cyc2_1)
STII : DRB96_29850 DRB96_30335
KIT : CFP65_0675 CFP65_6829
SPIQ : OHA34_15550 OHA34_15730
NGN : LCN96_09125 LCN96_54695
SEN : SACE_3187 SACE_3977 SACE_4907
» show all
Taxonomy
Reference
Authors
Jiang J, He X, Cane DE.
Title
Geosmin biosynthesis. Streptomyces coelicolor germacradienol/germacrene D synthase converts farnesyl diphosphate to geosmin.
Journal
Sequence
Reference
Authors
Cane DE, He X, Kobayashi S, Omura S, Ikeda H
Title
Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase.
Journal
Reference
Authors
Jiang J, He X, Cane DE
Title
Biosynthesis of the earthy odorant geosmin by a bifunctional Streptomyces coelicolor enzyme.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 4.1.99.16