Entry
Name
2-iminoacetate synthase;
thiH (gene name)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
L-tyrosine 4-methylphenol-lyase (2-iminoacetate-forming)
Reaction(IUBMB)
L-tyrosine + S-adenosyl-L-methionine + NADPH = 2-iminoacetate + 4-methylphenol + 5'-deoxyadenosine + L-methionine + NADP+ + H+ [RN:
R10246 ]
Reaction(KEGG)
Substrate
Product
Comment
Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule. The first stage of catalysis is reduction of the S-adenosyl-L-methionine to produce methionine and a 5-deoxyadenosin-5-yl radical that is crucial for the conversion of the substrate. The reductant is assumed to be NADPH, which is provided by a flavoprotein:NADPH oxidoreductase system [4]. Part of the pathway for thiamine biosynthesis.
History
EC 4.1.99.19 created 2011, modified 2014
Pathway
Orthology
K03150 2-iminoacetate synthase
Genes
ECOO : ECRM13514_5110(thiH)
ECOH : ECRM13516_4846(thiH)
ELR : ECO55CA74_23005(thiH)
ECW : EcE24377A_4531(thiH)
EDJ : ECDH1ME8569_3849(thiH)
ECOI : ECOPMV1_02800(thiH)
SEM : STMDT12_C42960(thiH)
SETC : CFSAN001921_19635(thiH)
SEEN : SE451236_00105(thiH)
SENH : CFSAN002069_11320(thiH)
SEEH : SEEH1578_06975(thiH)
SENJ : CFSAN001992_12965(thiH)
SEEC : CFSAN002050_03610(thiH)
SEEB : SEEB0189_021565(thiH)
SENC : SEET0819_04590(thiH)
REE : electrica_04816(thiH)
RTG : NCTC13098_06937(thiH_1) NCTC13098_06938(thiH_2)
CLAP : NCTC11466_04350(thiH)
KIE : NCTC12125_03515(thiH)
AHN : NCTC12129_04662(thiH_2)
METY : MRY16398_53710(thiH)
PSHI : SAMEA2665130_3032(thiH)
YPG : YpAngola_A0450 YpAngola_A3488
YPI : YpsIP31758_3857(thiH)
SMW : SMWW4_v1c02850(thiH)
SERS : SERRSCBI_01285(thiH)
SFJ : SAMEA4384070_0268(thiH)
SOF : NCTC11214_02087(thiH)
SSAR : SSARUM_000249(thiH)
PAQU : DMB82_0001020(thiH)
PARL : PEC302110_37590(thiH)
DIC : Dpoa569_000208(thiH)
EHD : ERCIPSTX3056_677(thiH)
TPTY : NCTC11468_00192(thiH_1)
PHEI : NCTC12003_00399(thiH)
PRJ : NCTC6933_00455(thiH)
LRI : NCTC12151_00406(thiH)
ALIG : NCTC10568_01005(thiH)
VAU : VANGNB10_cI2684c(thiH)
VPL : SA104470976_02675(thiH)
VIB : VspSTUT11_00270(thiH)
VIS : VspSTUT16_00300(thiH)
SON : SO_2440(thiH) SO_3923(hydG)
SHE : Shewmr4_1886 Shewmr4_3253
SHN : Shewana3_0708 Shewana3_1941
SHF : CEQ32_05050(thiH) CEQ32_19965
SBJ : CF168_10995(thiH) CF168_17250
SALG : BS332_04455 BS332_17515
SCAA : TUM17387_19050(thiH) TUM17387_33960(hydG)
SXM : MKD32_03725(hydG) MKD32_09700(thiH)
SSEH : N7V09_13605(thiH) N7V09_19900(hydG)
SCHK : GII14_10870(thiH) GII14_19210(hydG)
SDEO : D0436_04955(hydG) D0436_09965(thiH)
SHEL : JM642_03830(hydG) JM642_10575(thiH)
AEL : NCTC12917_00357(thiH)
CJV : MTVDSCj20_1046(thiH)
CFV : CFVI03293_0076(thiH)
CAMP : CFT03427_0081(thiH)
CCOC : CCON33237_0909(thiH)
CLR : UPTC16701_1120(thiH)
CLM : UPTC16712_1151(thiH)
CURE : CUREO_0458 CUREO_0574(thiH)
CHYO : CHH_0074 CHH_1391(thiH)
CHV : CHELV3228_0785(thiH)
CAMZ : CVIC12175_0606(thiH)
CRX : CRECT_1045(thiH) CRECT_1408
PCA : Pcar_0340(thiH-1) Pcar_0608(thiH-2) Pcar_1631(hydG)
PACE : A6070_01750 A6070_07115(thiH) A6070_12675
PEF : A7E78_02240 A7E78_08625
DDS : Ddes_1505 Ddes_1946 Ddes_2167
DFI : AXF13_01445(thiH) AXF13_01555 AXF13_14705
DPG : DESPIGER_1243 DESPIGER_1378 DESPIGER_1675
DEF : CNY67_01250 CNY67_02425 CNY67_09545
PSYF : N1030_02260(thiH) N1030_08265(hydG) N1030_15400(hydG)
DSB : LN040_00195(thiH) LN040_06035(hydG) LN040_08645(hydG)
DDE : Dde_1148 Dde_1556 Dde_2278
DSA : Desal_0367 Desal_1335 Desal_2649
DHY : DESAM_21863 DESAM_22766
DAS : Daes_0299 Daes_0596 Daes_1268
PPRF : DPRO_1002 DPRO_1177(thiH)
PSEL : GM415_11365(thiH) GM415_17555(hydG)
PSEF : PSDVSF_09050(thiH) PSDVSF_13940(hydG_1) PSDVSF_31410(hydG_2)
PPOR : JCM14722_06400(thiH) JCM14722_10500(hydG)
PNW : SYK_10380(thiH) SYK_30840(hydG)
PTHE : LF599_02800(hydG) LF599_11520(thiH)
PBIZ : LWC08_11115(hydG) LWC08_13540(thiH)
DVU : DVU_1765 DVU_2093(thiH)
DVG : Deval_1533 Deval_1945
DOA : AXF15_03270(thiH) AXF15_10055
THEW : TDMWS_09480(thiH) TDMWS_19730
DPS : DP0476 DP1740 DP2377
DPR : Despr_1899 Despr_1926
DWD : DSCW_39390(thiH_1) DSCW_62780(thiH_2)
DALK : DSCA_39370(thiH_1) DSCA_59010(thiH_2)
DEK : DSLASN_17030(thiH) DSLASN_44740(hydG)
SFU : Sfum_0842 Sfum_1822 Sfum_1841
ABIW : Abiwalacus_12760(thiH)
VBS : EGM51_06760(hydG) EGM51_16710(thiH)
PBU : L21SP3_00308(thiH_1) L21SP3_01127(thiH_2)
PBP : STSP1_00008(thiH_1) STSP1_01182(thiH_2)
PBAS : SMSP2_01583(thiH_1) SMSP2_01993(thiH_2)
ALUS : STSP2_00562(thiH_1) STSP2_01436(thiH_2)
VBL : L21SP4_01737(thiH_1) L21SP4_01856(thiH_2)
TAER : GT409_08540(thiH) GT409_10630(hydG)
VBC : C5Q97_06105(hydG) C5Q97_12495
STA : STHERM_c01460 STHERM_c18000
STQ : Spith_0134 Spith_0486
SPER : EW093_10235(hydG) EW093_14535(thiH)
TAZ : TREAZ_2036(thiH) TREAZ_2977
TAQU : KDW03_03815(thiH) KDW03_08625(hydG)
THYD : TTHT_0464 TTHT_1300
BTHO : Btheta7330_01174(thiH_1) Btheta7330_02231(thiH_2)
BFS : BF9343_2481(thiH) BF9343_3565
BFG : BF638R_2551(thiH) BF638R_3735
BFB : VU15_11030(thiH) VU15_17410(thiH)
BHL : Bache_2147 Bache_2364
BOA : Bovatus_00988(thiH_1) Bovatus_01896(thiH_2)
BCEL : BcellWH2_04992(thiH_1)
BCAC : CGC64_12260(thiH) CGC64_17110(hydG)
BCAE : A4V03_09505 A4V03_12785
BZG : C4H11_01730(thiH) C4H11_02305(hydG)
BHF : C3V43_03055(thiH) C3V43_03940(hydG)
BUN : Bun01g_25260(thiH_1) Bun01g_27300(thiH_2)
BFE : K6V30_15200(thiH) K6V30_22580(hydG)
BNO : K8P02_06500(thiH) K8P02_12915(hydG)
BHUM : JXR92_011905(hydG) JXR92_012835(thiH)
BFC : BacF7301_19625(thiH) BacF7301_24095(hydG)
BSAL : K6V25_16300(thiH) K6V25_20080(hydG)
BFIN : BFINE_34740 BFINE_53870(thiH)
BACE : H1A11_05640(hydG) H1A11_06580(thiH)
BDO : EL88_10570(thiH) EL88_21195(thiH)
BDH : GV66_01985(thiH) GV66_18380(thiH)
PCOP : I6J50_04855(thiH) I6J50_05945(hydG)
PAH : Poras_0729 Poras_0996
PCAG : NCTC12856_00571(thiH_1) NCTC12856_01717(thiH_3)
PSOE : CE91St14_11350(thiH)
CFAS : Cfast33896_08170(thiH)
OSP : Odosp_0630 Odosp_3276
BUY : D8S85_01395(thiH) D8S85_19145(hydG)
BVIR : I6J59_07535(hydG) I6J59_13455(thiH)
BFH : F1611_01810(hydG) F1611_07990(thiH)
PCLR : PC1C4_01210(thiH_1) PC1C4_15650(thiH_2)
HLAM : K4L44_07385(thiH) K4L44_10200(hydG)
HXY : K5X82_02135(thiH) K5X82_04890(hydG)
ASX : CDL62_01520 CDL62_13035(thiH)
SMIZ : 4412673_01356(thiH)
SDJ : NCTC13534_02113(thiH)
STHA : NCTC11429_04811(thiH)
OHM : MSHRCOH1_02810(thiH)
CGLE : NCTC11432_03026(thiH)
CHRJ : CHRYMOREF3P_2614(thiH)
CPO : COPRO5265_0141(thiH)
PSAB : PSAB_08650 PSAB_11965(thiH)
PDU : PDUR_09875 PDUR_13620
PGM : PGRAT_10860 PGRAT_17925
PAEQ : R50912_06590 R50912_11015
PSTE : PSTEL_10085 PSTEL_13385
PAEA : R70723_10635 R70723_15805
PAEE : R70331_10305 R70331_15245
PRI : PRIO_2354 PRIO_3930(thiH)
PSON : JI735_20530(thiH) JI735_26695(hydG)
PSOP : KP014_12375(thiH) KP014_16780(hydG)
PTJ : JRJ22_08930(thiH) JRJ22_12805(hydG)
PMAE : LMZ02_14935(hydG) LMZ02_26990(thiH)
CAC : CA_C1356(thiH) CA_C2921(thiH)
CAE : SMB_G1379(thiH) SMB_G2957(thiH)
CAY : CEA_G1370(thiH) CEA_G2928
CPF : CPF_0106 CPF_1853(thiH)
CPR : CPR_0108 CPR_1570(thiH)
CTC : CTC_01326(thiH) CTC_01748(thiH)
CTET : BN906_01430(thiH) BN906_01895
CNO : NT01CX_1438(thiH) NT01CX_1485
CBN : CbC4_0657(thiH) CbC4_0678
CBZ : Cbs_1520(thiH1) Cbs_4439(thiH2)
CBEI : LF65_01734 LF65_04975(thiH)
CKL : CKL_1636(thiH) CKL_3829(hydG)
CCB : Clocel_0684 Clocel_3433
CLB : Clo1100_2986 Clo1100_3825
CSR : Cspa_c46690(hydG) Cspa_c49000(thiH)
CPAS : Clopa_1126 Clopa_4120
CPAT : CLPA_c11910(thiH1) CLPA_c33350(thiH2)
CPAE : CPAST_c11910(thiH1) CPAST_c33350(thiH2)
CSB : CLSA_c07640(thiH1) CLSA_c25130(thiH2)
CLT : CM240_2381(thiH) CM240_3050
CBV : U729_165(hydG) U729_166(hydG)
CLD : CLSPO_c21810(thiH) CLSPO_c37660(hydG)
CACE : CACET_c35700(hydG) CACET_c37730(thiH)
CBUT : ATN24_11565 ATN24_14785(thiH)
CTYK : CTK_C12380(thiH) CTK_C21780
CEU : A7L45_01990 A7L45_04990
CDRK : B9W14_06315 B9W14_10270
CDY : F3K33_07895(thiH) F3K33_22740(hydG)
CCOH : SAMEA4530647_1147(thiH_1)
CMAH : C1I91_06870 C1I91_15320(hydG)
CRW : CROST_019270(cofH) CROST_037810(thiH)
CFB : CLFE_009920(thiH) CLFE_019240(cofH)
CAUN : CLAUR_010630(cofH) CLAUR_037480(thiH)
CNN : CNEO_2029(thiH) CNEO_3982
CGEL : psyc5s11_15150(thiH_1) psyc5s11_48500(thiH_2)
CTAG : LL095_02615(hydG) LL095_05575(thiH)
CINT : HZF06_00925(hydG) HZF06_05740(thiH)
CLOL : ONV75_11115(thiH) ONV75_13995(hydG)
CRS : FQB35_00650(thiH) FQB35_03925(hydG)
CPRF : K7H06_03145(hydG) K7H06_06115(hydG) K7H06_12520(thiH)
CRAS : KVH43_01515(hydG) KVH43_04000(thiH)
SARJ : HH195_05665(thiH) HH195_05695(hydG)
OCW : OW730_17785(thiH) OW730_18945(hydG)
PROQ : P6M73_04775(thiH) P6M73_16510(hydG)
CLO : HMPREF0868_0738(thiH)
CTX : Clo1313_1571 Clo1313_1630
CCL : Clocl_1570 Clocl_2545
HSC : HVS_07275(thiH1) HVS_12680(thiH3)
RPAY : P0092_17000(thiH) P0092_20515(hydG)
EHA : Ethha_0141 Ethha_1497
RUJ : E5Z56_05910(hydG) E5Z56_11420(thiH)
RGV : NQ502_11745(thiH) NQ502_18625(hydG)
RUC : LCN94_00745(hydG) LCN94_04945(thiH)
CARL : PXC00_04895(thiH) PXC00_09660(hydG)
SMAN : C12CBH8_00940(thiH)
PETR : QKW49_08350(thiH) QKW49_20390(hydG)
CCEL : CCDG5_0123 CCDG5_1406
AGAT : RWV98_08825(hydG) RWV98_14845(thiH)
SALQ : SYNTR_0772 SYNTR_2082
DAI : Desaci_0303 Desaci_2488
DCA : Desca_0062 Desca_1532
PTH : PTH_0641(ThiH) PTH_0984(ThiH)
DRU : Desru_0136 Desru_1635 Desru_2235
TFR : BR63_18280(hydG) BR63_18825(thiH)
FWA : DCMF_14220 DCMF_18870
AACX : DEACI_0836 DEACI_1666
DGI : Desgi_0865 Desgi_0920
HCV : FTV88_1003(hydG) FTV88_1718(thiH)
EMT : CPZ25_007325(hydG) CPZ25_017205
ELIM : B2M23_09770 B2M23_11965
AWO : Awo_c00520(thiH1) Awo_c28130(thiH2)
AWI : CHL1_000030(thiH) CHL1_000695(hydG)
EUU : ADJ67_00170(thiH) ADJ67_02425
RRK : H8S51_011505(thiH) H8S51_011585(hydG)
CEV : LK421_05545(hydG) LK421_10750(thiH)
BYL : A4V09_04190 A4V09_20930
BPRO : PMF13cell1_01243(thiH_1) PMF13cell1_04612(thiH_2)
BWX : NQ550_01140(thiH) NQ550_09365(hydG)
BLAR : LC508_01280(hydG) LC508_21315(thiH)
BLUI : Blut17040_26420(thiH)
BCOC : BLCOC_36080(mqnC) BLCOC_51960(thiH)
CSCI : HDCHBGLK_00596(thiH_1) HDCHBGLK_02708(thiH_2)
CHYL : CE91St63_07330(thiH_1) CE91St63_29290(thiH_2)
BPRL : CL2_17530 CL2_21490
ARF : AR1Y2_1226 AR1Y2_2982
ACAC : EYQ97_03415(hydG) EYQ97_11220(thiH)
ACEL : acsn021_08630(thiH)
ACHT : bsdcttw_39470(thiH)
RLR : NQ541_10500(hydG) NQ541_11115(thiH)
WHJ : H9Q79_09900(thiH) H9Q79_10195(hydG)
ERE : EUBREC_0223 EUBREC_0235
CBIL : EUBC25_07820(thiH) EUBC25_08390(thiH)
CPRO : CPRO_14530(thiH_1) CPRO_15950(thiH_3)
ANV : RBQ60_09110(hydG) RBQ60_12145(thiH)
VGU : HYG85_03350(thiH) HYG85_05910(hydG)
VPY : HZI73_01490(hydG) HZI73_14345(thiH)
ETM : CE91St48_30030(thiH_1) CE91St48_39530(thiH_2)
EMJ : V1225_02110(thiH) V1225_11940(hydG)
CSPO : QNI18_00085(hydG) QNI18_01035(hydG) QNI18_06830(thiH)
PBIF : KXZ80_02655(thiH) KXZ80_05620(hydG)
CDF : CD630_17050(thiH) CD630_21560(thiH)
PDC : CDIF630_01893(thiH1) CDIF630_02387(thiH2)
CDC : CD196_1629(thiH) CD196_2019
CDL : CDR20291_1604(thiH) CDR20291_2062
PDF : CD630DERM_17050(thiH) CD630DERM_21560(thiH)
ROC : HF520_00625(hydG) HF520_01930(hydG)
RHOM : FRIFI_0318 FRIFI_1134
PHX : KGNDJEFE_01024(thiH)
TEM : JW646_04960(thiH) JW646_05870(hydG)
TMY : TEMA_36800(mqnC) TEMA_38690(thiH)
TPET : TPHSE_06900(mqnC) TPHSE_08820(thiH)
THYR : P4S50_01035(hydG) P4S50_07440(thiH) P4S50_14160(thiH)
IBR : NQ514_04740(hydG) NQ514_09395(thiH)
PEB : O0R46_00085(thiH) O0R46_07470(hydG)
EAC : EAL2_808p03510(thiH) EAL2_c00360(thiH1) EAL2_c04210(thiH2)
FAA : HMPREF0389_01137(hydG)
TKI : TKV_c05810(thiH1) TKV_c08370(thiH2) TKV_c11110(thiH3)
CHY : CHY_1263(thiH) CHY_1549
AAUT : ACETAC_07945(hydG) ACETAC_08285(thiH)
MTHO : MOTHE_c16710(thiH1) MOTHE_c17450(thiH2)
MTHZ : MOTHA_c17540(thiH1) MOTHA_c18280(thiH2)
AAR : Acear_1233 Acear_1572
HHL : Halha_1308 Halha_2625
AFT : BBF96_07240 BBF96_09105
HPRF : HLPR_06960(hydG) HLPR_19360(thiH)
AVG : I6H45_05445(hydG) I6H45_07435(thiH)
PIV : NCTC13079_00531(thiH_2) NCTC13079_01343(thiH_3)
KPAR : JL105_01605(hydG) JL105_02555(thiH)
CAZO : G3A45_00590(thiH) G3A45_06760(hydG)
VRM : 44547418_01296(thiH)
VDN : NCTC11831_00662(thiH)
SSG : Selsp_0447 Selsp_0454
SRI : SELR_04770 SELR_06120(thiH) SELR_07930
MFUN : GXM21_01735(hydG) GXM21_06375(thiH)
SGBI : P3F81_03805(thiH) P3F81_04880(hydG)
MANA : MAMMFC1_03376(thiH_1) MAMMFC1_03516(thiH_2)
STED : SPTER_22510(thiH) SPTER_24200(bioB_3)
SSPH : SPSPH_024790(mqnC_2) SPSPH_039400(thiH)
SOVA : SOV_29250(thiH_1) SOV_44640(thiH_2)
SSID : SPSIL_032680(mqnC_2) SPSIL_048370(thiH)
SACV : SPACI_033390(mqnC_2) SPACI_046630(thiH_1) SPACI_056250(thiH_2)
AIN : Acin_0255(thiH) Acin_2104(thiH)
PFAC : PFJ30894_00306(thiH_1) PFJ30894_01916(thiH_3)
COB : COB47_0160 COB47_0337
CHD : Calhy_2278 Calhy_2456
COW : Calow_0107 Calow_0240
CKI : Calkr_0147 Calkr_0323
CLC : Calla_2034 Calla_2181
CKN : Calkro_2258 Calkro_2401
CCHA : ELD05_01270(thiH) ELD05_12785(hydG)
CDIA : CaldiYA01_20710(thiH_1) CaldiYA01_22400(thiH_2)
CNAG : OTJ99_000195(hydG) OTJ99_002246(thiH)
CMOR : OTK00_000200(thiH) OTK00_002301(hydG)
CDAN : SOJ16_000176(hydG) SOJ16_000320(thiH)
RAIN : Rai3103_14105(thiH)
TFE : JYK00_08085(hydG) JYK00_09435
OCY : OSSY52_09820(thiH_1) OSSY52_15590(thiH_2)
MPZ : Marpi_0104 Marpi_0889
MARN : LN42_00760(thiH) LN42_04890
MAEO : JRV97_03770 JRV97_08895(hydG)
FVA : FV113G1_12650 FV113G1_17910(thiH)
FUL : C4N20_12155 C4N20_13285
FMO : C4N19_03965 C4N19_10305
FGO : C4N16_03845 C4N16_07440
FNF : BSQ88_02560(thiH) BSQ88_07095(hydG)
FHO : H9Q81_03400(thiH) H9Q81_06460(hydG)
FPOL : ERS445057_00302(thiH)
IPO : Ilyop_0481 Ilyop_1184
CSOM : MKD34_10695(hydG) MKD34_11455(thiH)
HABY : HLVA_10870(thiH_1) HLVA_21230(thiH_2)
» show all
Taxonomy
Reference
Authors
Leonardi R, Fairhurst SA, Kriek M, Lowe DJ, Roach PL
Title
Thiamine biosynthesis in Escherichia coli: isolation and initial characterisation of the ThiGH complex.
Journal
Sequence
Reference
Authors
Kriek M, Martins F, Challand MR, Croft A, Roach PL
Title
Thiamine biosynthesis in Escherichia coli: identification of the intermediate and by-product derived from tyrosine.
Journal
Sequence
Reference
Authors
Kriek M, Martins F, Leonardi R, Fairhurst SA, Lowe DJ, Roach PL
Title
Thiazole synthase from Escherichia coli: an investigation of the substrates and purified proteins required for activity in vitro.
Journal
Sequence
Reference
Authors
Challand MR, Martins FT, Roach PL
Title
Catalytic activity of the anaerobic tyrosine lyase required for thiamine biosynthesis in Escherichia coli.
Journal
Other DBs
ExPASy - ENZYME nomenclature database: 4.1.99.19