KEGG   ENZYME: 4.1.99.26
Entry
EC 4.1.99.26                Enzyme                                 
Name
3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one synthase;
mftC (gene name)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one lyase (C-terminal [mycofactocin precursor peptide]-glycyl-L-valyl-4-[2-aminoethenyl]phenol-forming)
Reaction(IUBMB)
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + 5'-deoxyadenosine + L-methionine + A = C-terminal [mycofactocin precursor peptide]-glycyl-L-valyl-4-[2-aminoethenyl]phenol + S-adenosyl-L-methionine + AH2 [RN:R12750]
Reaction(KEGG)
R12750
Substrate
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one [CPD:C22365];
5'-deoxyadenosine [CPD:C05198];
L-methionine [CPD:C00073];
A [CPD:C00028]
Product
C-terminal [mycofactocin precursor peptide]-glycyl-L-valyl-4-[2-aminoethenyl]phenol [CPD:C22345];
S-adenosyl-L-methionine [CPD:C00019];
AH2 [CPD:C00030]
Comment
This is a bifunctional radical AdoMet (radical SAM) enzyme that catalyses the first two steps in the biosynthesis of the enzyme cofactor mycofactocin. Activity requires the presence of the MftB chaperone. The reaction occurs in the right-to-left direction. The other activity of the enzyme is EC 1.3.98.7, [mycofactocin precursor peptide]-tyrosine decarboxylase.
History
EC 4.1.99.26 created 2021
Orthology
K24696  [mycofactocin precursor peptide]-tyrosine decarboxylase / 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one synthase
Genes
PVBJ5X90_01710
BURKDM992_04425
URUDSM104443_01508(mftC)
UPLDSM104440_02432(moaA_2) DSM104440_02434(mftC)
WEELOK48_06000
SPAPH3Z74_12905
DEXHWD60_01690(mftC)
DVNHQ394_08780(mftC)
GPIGPICK_06635
GEBGM18_3219
GURGura_3559
DLIdnl_37460(mftC)
AORYAMOR_45640(mftC)
MYXQEG98_18495
PSONJI735_28730
PMAELMZ02_27280
ECECNCTC12421_02257(moaA)
PTHPTH_0594
DAEDtox_4272
ACHTbsdcttw_26530
MTURv0693
MTVRVBD_0693
MTCMT0720
MRAMRA_0701(pqqE)
MTFTBFG_10707
MTBTBMG_00706
MTKTBSG_00710
MTZTBXG_000699
MTIMRGA423_04325
MTECCDC5079_0644
MTURCFBS_0729(pqqE)
MTLCCDC5180_0635
MTOMTCTRI2_0709(pqqE)
MTDUDA_0693(pqqE)
MTNERDMAN_0765(pqqE)
MTJJ112_03710
MTUBMT7199_0711
MTUEJ114_03700
MTXM943_03605
MTULTBHG_00688
MTUTHKBT1_0729(pqqE)
MTUUHKBT2_0730(pqqE)
MTQHKBS1_0729(pqqE)
MBOBQ2027_MB0712(pqqE)
MBBBCG_0742(pqqE)
MBTJTY_0712(pqqE)
MBMBCGMEX_0713(pqqE)
MBKK60_007390
MBXBCGT_0485
MAFMAF_07020(pqqE)
MMICRN08_0773
MCEMCAN_06941(pqqE)
MCQBN44_10763(pqqE)
MCVBN43_20125(pqqE)
MCXBN42_20448(pqqE)
MCZBN45_10793(pqqE)
MORYMO_000738(mftC)
MPAMAP_4153(pqqE)
MAOMAP4_4279
MAVIRC58_21255
MAVURE97_21305
MAVMAV_4478
MITOCO_43690
MIAOCU_43450
MIDMIP_06630
MYOOEM_43920
MCHIAN480_22770
MIROCQ_44800
MMALCKJ54_20795(mftC)
MLPMLM_3634
MMANMMAN_31920(mftC)
MSAMycsm_00993
MULMUL_0773(pqqE)
MMIMMAR_1021(pqqE)
MMAEMMARE11_09740(pqqE)
MLIMULP_01148(pqqE)
MPSEMPSD_11580(pqqE)
MSHOMSHO_28100(mftC)
MMCMmcs_1001
MKMMkms_1018
MJLMjls_1028
MMMW7S_22025
MKNMKAN_19590
MYVG155_07135
MYEAB431_05550
MHADB586_18795
MDXBTO20_28590
MSHGMSG_00882(mftC)
MFJMFLOJ_25800(mftC)
MSTOMSTO_29440(mftC)
MSIMMSIM_16080(mftC)
MSAKMSAS_44570(pqqE)
MKUI2456_05200(mftC)
MLWMJO58_04700(mftC)
MXEMYXE_39990
MNVMNVI_25240
MPAGC0J29_05580(mftC)
MNMMNVM_27250
MGORH0P51_05075(mftC)
MCOOMCOO_41760
MBAIMB901379_00882(albA)
MSEOMSEO_48250(pqqE)
MHEKJMUB5695_03904
MGAUMGALJ_54730
MLJMLAC_34950(pqqE)
MBRDMBRA_06490(mftC)
MSHJMSHI_27520(mftC)
MMAMK3U93_04265(mftC)
MSPGF6B93_04315(mftC)
MOTLTS72_20560(mftC)
MPAAMKK62_01650(mftC) MKK62_20125(mftC)
MMEHM5I08_04070(mftC) M5I08_21200(mftC)
MKYIWGMT90018_10150(pqqE)
MADIA7U43_25310
MWUPT015_22325(mftC)
MSMMSMEG_1423
MSGMSMEI_1388(pqqE)
MSBLJ00_07095
MSNLI99_07095
MSHLI98_07095
MVAMvan_1296
MGIMflv_5060
MSPMspyr1_44780
MCBMycch_0934
MNED174_06605
MYNMyAD_06470
MFTXA26_14270
MPHLMPHLCCUG_01293(albA)
MVQMYVA_1101
MLLB1R94_05275
MRHMycrhN_0708
MTHN4412656_00903(moaA_1)
MHASMHAS_00222(mftC)
MDUMDUV_34210
MCHTMCHIJ_01200
MDRMDOR_06280
MAUUNCTC10437_01079(moaA_1)
MMAGMMAD_11150
MMORMMOR_54180
MFXMFAL_21360
MAICMAIC_45870
MIJMINS_13330
MALVMALV_24330
MTYMTOK_31920
MPSCMPSYJ_53850
MARZMARA_27880
MGADMGAD_28140
MHEVMHEL_11880
MSARMSAR_25550
MANYMANY_49630
MAUBMAUB_22120
MPOFMPOR_15780
MPHUMPHO_48440
MMUCC1S78_023005(mftC)
MMATMMAGJ_31370 MMAGJ_42610
MBOKMBOE_62330
MFGK6L26_25505(mftC)
MSEIMSEDJ_23770
MFLVNCTC10271_04120(moaA_2)
MCEEMCEL_23190
MMONEWR22_05390(mftC)
MGOAFA91_31800
MHOLK3U96_21205(mftC)
MSENK3U95_06215(mftC)
MPAEK0O64_05085(mftC)
MRFMJO55_05335(mftC)
MDFK0O62_05970(mftC)
MCROMI149_05810(mftC)
MGROFZ046_11350(mftC)
MKRMKOR_22780
MPAKMIU77_15075(mftC)
MABMAB_3835c
MMVMYCMA_2121
MABBMASS_3846
MABLMMASJCM_3850
MCHEBB28_19635
MIZBAB75_21910
MSTEMSTE_03984
MSAOMYCSP_17705
MSALDSM43276_03628(albA_1)
MJDJDM601_3348(pqqE)
MTER4434518_03335(pqqE)
MMINMMIN_10500
MHIBMHIB_27760
MHERK3U94_19120(mftC)
MVMMJO54_19085(mftC)
ASDAS9A_4328 AS9A_4370
NFANFA_22330 NFA_34430
NFRERS450000_00607(moaA_1) ERS450000_01864(moaA_2)
NCYNOCYR_4848
NBRO3I_038110
NNONONO_c43730
NSLBOX37_28985
NOZDMB37_16920
NAHF5544_40635(mftC)
NADNCTC11293_06440(moaA_2)
NWLNWFMUON74_31340
NIEKV110_01685 KV110_37300(mftC)
NGPLTT66_16810(mftC) LTT66_27865
NSPUIFM12276_04450
RHARHA1_ro06072
RERRER_17990
REYO5Y_08630
REBXU06_08870
RQIC1M55_09105(mftC)
ROPROP_61300
ROAPd630_LPD02713
RPYY013_08775
RBYCEJ39_19670(mftC)
RHBNY08_1952
RAVAAT18_18590
RFAA3L23_00297(mftC)
RHSA3Q41_03117(mftC)
RHWBFN03_03195
RRZCS378_08060(mftC)
RHUA3Q40_00412(mftC)
RHQIM25_00605
RHODAOT96_19470
RRT4535765_03565(moaA_2)
RCRNCTC10994_03687(moaA_1)
RTMG4H71_16285(mftC)
RKOJWS14_33110(mftC)
RGOKYT97_09560(mftC)
ROZCBI38_20395
RPSKJWS13_10850(mftC)
RANTRHODO2019_14325(mftC)
RHOPD8W71_12595(mftC)
REQREQ_36270
PDEFP9209_16155(mftC)
WHROG579_07510(mftC)
GBRGbro_3778
GPOGPOL_c36480
GORKTR9_3737
GOQACH46_15915
GTABCM27_19570
GOCCXX93_06955(mftC)
GITC6V83_16215(mftC)
GRUGCWB2_19180(albA)
GOMD7316_04926(mftC)
GAVC5O27_08960(mftC)
GODGKZ92_18110(mftC)
GAMGII34_18050(mftC)
GPDGII33_17505(mftC)
GJIH1R19_17930(mftC)
GOILK459_15805(mftC)
GHNMVF96_18495(mftC)
GAMIIHQ52_22085(mftC)
GMGNWF22_03545(mftC)
GSIP5P27_15285(mftC)
SPINKV203_02225(mftC)
TSMASU32_04090
TSDMTP03_02840
DTMBJL86_2336
DITC3V38_01380(mftC)
DIZCT688_11595(mftC)
DPCA6048_12175
DLUA6035_11195
DKNNHB83_11615(mftC)
TOYFO059_05810(mftC)
SHYSHJG_2443
SHOSHJGH_2208
SBROGQF42_07985(mftC)
STUIGCM10017668_53920
SPRACP972_11025(mftC)
SYANNRK68_35035(mftC)
SCIRSTRCI_007929(mftC)
SANTQR300_11390(mftC)
YIALO772_03300(mftC)
MSEDE3O41_00690(mftC)
MSFIT882_01150(mftC)
MCAWF6J84_03070(mftC)
MELYL2X98_31250(mftC)
FRPAX769_01345
FSBGCM10025867_43650
AMAVGCM10025877_11620
CPHYB5808_07270
CHREIE160_03000(mftC)
HUMDVJ78_13615(mftC)
LALLMUN78_02670(mftC)
HEAHL652_05825(mftC)
SUBALQ955_14985(mftC)
AJGKKR91_08595(mftC)
AZXN2K95_07805(mftC)
AJRN2K98_08415(mftC)
ALXLVQ62_05585(mftC)
BEIGCM100_01140
JLIEXU32_11045(mftC)
JAYH7A72_14560(mftC)
PHWG7075_01305(mftC)
NDPE2C04_03200(mftC)
NAROCFH99_21865(mftC) CFH99_24470(mftC)
NAQUENKNEFLB_00509(mftC)
NROK8W59_04795(mftC)
NPCKUV85_09740(mftC)
NPSKRR39_16395(mftC)
NMARHPC71_02600(mftC)
PSIMKR76_24775
AEZC3E78_15430(mftC)
AEBC6I20_03055(mftC)
AEFGEV26_02910(mftC)
NBTKLP28_13005(mftC)
NGVCDO52_16170(mftC)
SAIUJ4H86_09360(mftC)
TCUTcur_2362
ACTWF7P10_02075(mftC)
AMAZLUW76_43950(mftC)
FREFranean1_3326
FALFRAAL3572(pqqE)
FSYFsymDg_2656
FRIFraEuI1c_0997
NMLNamu_0775
NAKAH7F38_19400(mftC)
GOBGobs_4936
BSDBLASA_4812
MMARMODMU_5392
SENSACE_2389(pqqE)
SACGFDZ84_29565(mftC) FDZ84_34715(mftC)
AMQAMETH_4935(pqqE)
AMYCCU254_14360(mftC)
AACDLWP59_29720(mftC)
APRTMUY14_18355(mftC)
AROONQK81_28185(mftC)
ATHML1857_27430(mftC)
ARHDVSH64_10205(mftC)
PDXPsed_5681
PSEAWY02_13545
PSEHXF36_00070
PSEQAD006_08285
PECQAD017_16115
PHHAFB00_13925
PAUTPdca_61410
PBROHOP40_10800(mftC)
PPELH6H00_10965(mftC)
KBUQ4V64_47345(mftC)
ALOCRK56114
PMADBAY61_06015
ACTYOG774_15450(mftC)
ASEACPL_6044(pqqE)
AMSAMIS_27220
ACTSACWT_5912
ACTLL3i22_048340
CATICS0771_62030
EKEEK0264_07230(mftC)
RXYRxyl_3154
RRDRradSPS_2636
SBAEDSM104329_01719(mftC)
AYMYM304_35130
AQZKSP35_11420(mftC)
IAMHC251_02955(mftC)
DHILH044_09590(mftC)
PMETG4Y79_04760
TROtrd_A0840
STISthe_2856
BHCJFL75_07965
MSEAMETESE_08880
MICAP0L94_00205(mftC)
 » show all
Reference
1  [PMID:21223593]
  Authors
Haft DH.
  Title
Bioinformatic evidence for a widely distributed, ribosomally produced electron carrier precursor, its maturation proteins, and its nicotinoprotein redox partners.
  Journal
BMC Genomics 12:21 (2011)
DOI:10.1186/1471-2164-12-21
  Sequence
[mtu:Rv0693]
Reference
2  [PMID:27158836]
  Authors
Bruender NA, Bandarian V
  Title
The Radical S-Adenosyl-l-methionine Enzyme MftC Catalyzes an Oxidative Decarboxylation of the C-Terminus of the MftA Peptide.
  Journal
Biochemistry 55:2813-6 (2016)
DOI:10.1021/acs.biochem.6b00355
Reference
3  [PMID:28634235]
  Authors
Khaliullin B, Ayikpoe R, Tuttle M, Latham JA
  Title
Mechanistic elucidation of the mycofactocin-biosynthetic radical S-adenosylmethionine protein, MftC.
  Journal
J Biol Chem 292:13022-13033 (2017)
DOI:10.1074/jbc.M117.795682
  Sequence
[mul:MUL_0773]
Reference
4  [PMID:30628436]
  Authors
Ayikpoe R, Ngendahimana T, Langton M, Bonitatibus S, Walker LM, Eaton SS, Eaton GR, Pandelia ME, Elliott SJ, Latham JA.
  Title
Spectroscopic and Electrochemical Characterization of the Mycofactocin Biosynthetic Protein, MftC, Provides Insight into Its Redox Flipping Mechanism.
  Journal
Biochemistry 58:940-950 (2019)
DOI:10.1021/acs.biochem.8b01082
  Sequence
[mul:MUL_0773]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.26
IUBMB Enzyme Nomenclature: 4.1.99.26
ExPASy - ENZYME nomenclature database: 4.1.99.26
BRENDA, the Enzyme Database: 4.1.99.26

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