KEGG ENZYME: 4.2.1.103

ENZYME: 4.2.1.103

Entry

EC 4.2.1.103                Enzyme

Name

cyclohexyl-isocyanide hydratase;
isonitrile hydratase;
N-cyclohexylformamide hydro-lyase

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

N-cyclohexylformamide hydro-lyase (cyclohexyl-isocyanide-forming)

Reaction(IUBMB)

N-cyclohexylformamide = cyclohexyl isocyanide + H2O [RN:R05771]

Reaction(KEGG)

R05771

Substrate

N-cyclohexylformamide [CPD:C11519]

Product

cyclohexyl isocyanide [CPD:C11520];
H2O [CPD:C00001]

Comment

The enzyme from Pseudomonas putida strain N19-2 can also catalyse the hydration of other isonitriles to the corresponding N-substituted formamides. The enzyme has no metal requirements.

History

EC 4.2.1.103 created 2001

Pathway

ec00930

Caprolactam degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K18199

cyclohexyl-isocyanide hydratase

Genes

KPE:	KPK_B0025(inhA)

KGR:	JJJ10_22360

SYM:	K6K13_11445

YAS:	N0H69_12835

SMAR:

SM39_0772

SMAC:

SMDB11_0595

SMW:	SMWW4_v1c13220

SRR:	SerAS9_1315

SRL:	SOD_c12300(inhA)

SRY:	M621_06870

SRS:	SerAS12_1315

SRA:	SerAS13_1315

SLQ:	M495_06150

SERF:

L085_21850

SERS:

SERRSCBI_06230

SERA:

Ser39006_016470

SERQ:

CWC46_16470

SFJ:	SAMEA4384070_1408

SSUR:

ATE40_003380

SURI:

J0X03_17185

SENP:

KHA73_06970

SNEM:

NLX84_06615

SFO:	Z042_02840

RAH:	Rahaq_2239

RAQ:	Rahaq2_2344

RAA:

Q7S_11325

ROX:	BV494_12435

RVC:	J9880_24115

GQU:	AWC35_05010

RBAD:

H2866_07755

RCB:	O1V66_04280

DDA:	Dd703_2109

LBQ:	CKQ53_13760

LPOP:

I6N93_07775

SOD:

Sant_2439

PES:	SOPEG_2323

EBI:

EbC_00490

ERP:	LJN55_23475

PAM:

PANA_1582

PLF:	PANA5342_2643

PAJ:

PAJ_0920

PAQ:	PAGR_g2559

PVA:	Pvag_pPag30009

PAGG:

AL522_00945

PAO:	Pat9b_5214

PANT:

PSNIH1_p00470

PAGC:

BEE12_21550

PANS:

FCN45_21235

PEY:	EE896_19710

PDIS:

D8B20_19625

PER:	LAC65_16270

PJI:	KTJ90_21540

PANO:

OJ965_00125

KPIE:

N5580_20590

PAY:

PAU_02206

LPV:	HYN51_05745

PFQ:	QQ39_04125

RGL:	CS053_12030

DTX:	ATSB10_30330

PST:	PSPTO_2847

PCI:	PCH70_32170

PCAB:

JGS08_13025

PAST:

N015_18645 N015_18655

PLIJ:

KQP88_15270

PFL:	PFL_4109(inhA)

PPRC:

PFLCHA0_c41680

PPRO:

PPC_4210

PFE:	PSF113_1826(inhA)

PFN:	HZ99_00150

PFO:	Pfl01_1835

PFS:

PFLU_2085

PFC:	PflA506_1987(inhA)

PPZ:	H045_06030

PFB:

VO64_1327

PMAN:

OU5_1432

PTV:	AA957_04435

PCG:	AXG94_06590

PVR:	PverR02_10275

PAZO:

AYR47_17100

POI:	BOP93_09185

PRX:	HRH33_10535

PMED:

E3Z27_19160

PFW:	PF1751_v1c19050(inhA)

PFF:	PFLUOLIPICF727195

PFX:	A7318_08895

PLUL:

FOB45_02625

PBA:	PSEBR_a3931

PBC:	CD58_20725

PPUU:

PputUW4_01540

PSK:	U771_10845

PCH:	EY04_20820

PCZ:	PCL1606_20730

PCP:	JM49_10390

PSEM:

TO66_21510

PSOS:

POS17_4209

PKR:	AYO71_12120

PFK:	PFAS1_02640

PANR:

A7J50_2095

PSIL:

PMA3_20215

PYM:	AK972_1776

PKE:	DLD99_10010

PUM:	HGP31_22190

PGG:	FX982_03252

PGY:	AWU82_02140

PVK:	EPZ47_19870

PEZ:	HWQ56_22285

PBZ:	GN234_27555

PATA:

JWU58_18155

PZE:	HU754_010780

PMOE:

HV782_010360

PSEP:

C4K39_6042

PVW:	HU752_021385

PTRT:

HU722_0010610

PSAM:

HU731_025725

PTZ:	HU718_010460

PQI:	KH389_07090

PSHH:

HU773_010815

PHV:	HU739_008405

PASG:

KSS96_10145

PAZE:

KSS91_08695

PALV:

KSS97_09660

PZA:	HU749_020645

PIE:	HU724_009930

PGF:	J0G10_10575

PIZ:	LAB08_R42270

PPEG:

KUA23_10235 KUA23_15205

PSII:

NF676_09875

PCAS:

LOY40_08930

PFIT:

KJY40_10535

AVZ:	HWI77_19085

SAVI:

JYB87_17060

SHEJ:

MZ182_19130

MOQ:	HWV03_11270

MMAI:

sS8_4514

TSB:	HMY34_02050

KUY:	FY550_11460

ADI:

B5T_01317

AXE:

P40_06200

SALN:

SALB1_2907

CDIZ:

CEDIAZO_00144(inhA)

CVI:

CV_1313

CVC:	BKX93_19500

CHRI:

DK842_14120

CHRB:

DK843_19690

RSL:	RPSI07_mp0745

RSN:	RSPO_m01124

RPI:

Rpic_3890

RPF:	Rpic12D_4003

RIN:	ACS15_5592(inhA)

RSG:	JK151_21505

RWE:	KOL96_03755

CNC:	CNE_BB1p06340 CNE_BB1p06350 CNE_BB1p06390

REU:	Reut_C5977

CCUP:

BKK81_24965

CUP:	BKK80_31400

CUU:	BKK79_22285

COX:	E0W60_28170 E0W60_30800 E0W60_30825

BMA:

BMAA0232

BMV:	BMASAVP1_1415

BML:	BMA10229_1610

BMN:	BMA10247_A0269

BMAL:

DM55_3487

BMAE:

DM78_4670

BMAQ:

DM76_4184

BMAI:

DM57_14220

BMAF:

DM51_3982

BMAZ:

BM44_3449

BMAB:

BM45_4285

BPS:

BPSS1863

BPM:	BURPS1710b_A0954

BPL:	BURPS1106A_A2525

BPD:	BURPS668_A2666

BPSE:

BDL_5266

BPSM:

BBQ_4269

BPSU:

BBN_5326

BPSD:

BBX_4431

BPZ:	BP1026B_II1996

BPQ:	BPC006_II2486

BPK:

BBK_4598

BPSH:

DR55_5874

BPSA:

BBU_4148

BPSO:

X996_5671

BUT:

X994_5235

BTE:	BTH_II0514

BTQ:

BTQ_3807

BTJ:

BTJ_4838

BTZ:

BTL_5633

BTD:

BTI_5131

BTV:

BTHA_4577

BTHE:

BTN_5295

BTHM:

BTRA_5566

BTHA:

DR62_5433

BTHL:

BG87_5640

BOK:

DM82_6139

BOC:

BG90_4526

BUU:	WS70_26755

BSAV:

WS86_28455

BHG:	I6G56_27295

BVI:	Bcep1808_2772

BVE:

AK36_1231

BUR:	Bcep18194_A5986 Bcep18194_C6949

BCM:	Bcenmc03_2687

BCH:	Bcen2424_2658

BCJ:

BCAL0944

BCEN:

DM39_2748

BCEW:

DM40_285

BCEO:

I35_2723

BAM:

Bamb_2705

BAC:	BamMC406_2579

BMJ:	BMULJ_02622

BMU:

Bmul_0639

BMK:

DM80_2260

BMUL:

NP80_2794

BCT:

GEM_0778

BCED:

DM42_2413

BCEP:

APZ15_01180 APZ15_37075

BDL:

AK34_430

BPYR:

ABD05_03325

BCON:

NL30_26365

BUB:

BW23_2234

BDF:	WI26_12850 WI26_18185

BLAT:

WK25_12910

BTEI:

WS51_23710

BSEM:

WJ12_13325

BPSL:

WS57_32075

BMEC:

WJ16_13640 WJ16_18360

BSTG:

WT74_14115

BSTL:

BBJ41_15470

BANN:

JFN94_13625 JFN94_18780

BARI:

NLX30_15460 NLX30_37835 NLX30_38635

BGL:	bglu_1g11570

BGU:

KS03_2093

BGD:	bgla_1g13580 bgla_4p2640

BGO:

BM43_2691

BYI:	BYI23_B003790(pfpI)

BUK:

MYA_2423

BUE:	BRPE67_BCDS06360(pfpI)

BUL:

BW21_4667

BGP:	BGL_1c12500

BPLA:

bpln_1g12010

BUD:	AQ610_29760

BUM:	AXG89_22605

BUI:	AX768_25090

BURK:

DM992_06690

BGE:	BC1002_3249

PSPW:

BJG93_19305

PARA:

BTO02_25970

PMEG:

FNZ07_17295 FNZ07_18000

PTRO:

G5S35_18420

PACP:

FAZ97_16555 FAZ97_22925

PACS:

FAZ98_21115

PEW:	KZJ38_28570

PBRY:

NDK50_34730

POX:	MB84_16600

PTX:	ABW99_09470 ABW99_09765

PCOM:

NTU39_13730

PLG:	NCTC10937_01916

CABA:

SBC2_66130(inhA_2)

BUO:	BRPE64_BCDS09480

BGM:	CAL15_04620 CAL15_16450

AXY:	AXYL_02520

AXO:	NH44784_041151

AXN:	AX27061_2302

AXX:	ERS451415_02297

ADT:	APT56_11265

AIS:	BUW96_02845

ASW:	CVS48_15890

ACHR:

C2U31_02555 C2U31_23205

ACHB:

DVB37_09800

ADY:	HLG70_11255

ACHO:

H4P35_10000

AMUI:

PE062_06710

AMIM:

MIM_c36260

ODI:

ODI_R3652

PACR:

FXN63_14005

AAV:

Aave_3882

LIH:	L63ED372_02726(inhA)

PBH:	AAW51_3464

ROI:	N4261_25460

MIU:	ABE85_09740

CFU:

CFU_1980

CPRA:

CPter91_1266

MNR:	ACZ75_09635

MFY:	HH212_09865

TMJ:	P0M04_10870

MAMO:

A6B35_06360

MESP:

C1M53_09140 C1M53_24005

PHT:	BLM14_21445

PHYL:

HB779_15435

PMOB:

HG718_06600

SFH:	SFHH103_06122

ARA:

Arad_8694

ATF:	Ach5_50350

AGT:	EYD00_13990 EYD00_23945

ARUI:

G6M88_05190

AVV:	RvVAT039_01740 RvVAT039_pl02010

AVI:	Avi_1644 Avi_7628

RTR:	RTCIAT899_PC03145

RJG:	CCGE525_28305

RHR:	CKA34_22720

RLS:	HB780_08580

RAW:	NE851_12590

NGL:	RG1141_CH10730

NGG:	RG540_CH10970

NEN:	NCHU2750_51300

KAI:	K32_01220 K32_14080

OIN:	IAR37_11580

BPSN:

NIK97_17885 NIK97_17890

BJA:	bll4280(bll4280)

BJU:	BJ6T_55320

BJP:	RN69_26815

BRA:	BRADO3481 BRADO4159

BBT:	BBta_4230 BBta_4536

BRS:	S23_30320 S23_40040 S23_56940

AOL:

S58_36400

BRC:	BCCGELA001_07170 BCCGELA001_17850

BRAD:

BF49_0765

BIC:	LMTR13_21705

BRO:	BRAD285_3854

BRK:	CWS35_00885 CWS35_16175 CWS35_22960 CWS35_35285

BOT:	CIT37_28250 CIT37_32700

BRQ:	CIT40_14000 CIT40_18185

BGQ:	X265_17920

BGZ:	XH91_19540 XH91_27890

BSYM:

CIT39_13170 CIT39_17535

BBET:

F8237_00370

BARH:

WN72_23560

BVZ:	BRAD3257_4752(inhA)

BEL:	BE61_60030 BE61_85190

BDG:	LPJ38_23320

BSEP:

HAP48_0021345 HAP48_0036700 HAP48_0044390

BQB:	J4P68_0012710 J4P68_0037910

BSEI:

KMZ68_12475

BBAN:

J4G43_024640

BCAN:

BcanWSM471_18760

RPA:

RPA2422

RPB:	RPB_2555 RPB_3031

RPC:

RPC_2887

RPT:

Rpal_2700

RPX:	Rpdx1_3085

RBK:	E0H22_15260

OCA:

OCAR_6811

OCG:	OCA5_c12650

OCO:	OCA4_c12650

VGO:	GJW-30_1_00522(inhA_1)

TRB:	HB776_04255

BVV:	BHK69_11790

BOI:	BLM15_04605 BLM15_18455

BOF:	FQV39_04355

BAPI:

BBC0122_024120

BAPA:

BBC0178_022590

XAU:

Xaut_1973

XDI:	EZH22_04670

AZC:

AZC_2741

LNE:	FZC33_17880

MEA:	Mex_1p0926(inh) Mex_1p3533(inh) Mex_1p4818(inh)

MDI:	METDI1727(inh) METDI4110(inh) METDI5414(inh)

MEX:	Mext_1154 Mext_3318 Mext_4368

MCH:	Mchl_1313 Mchl_3642 Mchl_4844

MPO:

Mpop_3518

MZA:	B2G69_03875 B2G69_13040 B2G69_24145

MRD:	Mrad2831_1653 Mrad2831_3391

MET:

M446_4875

MNO:

Mnod_1849

MOR:	MOC_2046 MOC_3899

META:

Y590_04890 Y590_16250

MAQU:

Maq22A_c27365(thiJ)

MPHY:

MCBMB27_01301 MCBMB27_02881

MEE:	DA075_03875 DA075_33305 DA075_33345

METD:

C0214_18295 C0214_24205

METX:

A3862_14490 A3862_22610

METS:

DK389_08825 DK389_09000 DK389_09835 DK389_27220

METI:

DK427_05315 DK427_16925

MMES:

MMSR116_01165 MMSR116_12260

MTEA:

DK419_05255

MIND:

mvi_12520 mvi_37970

MOG:	MMB17_16905

MTAD:

M6G65_09610 M6G65_31555

BID:

Bind_1474

MLG:	CWB41_14540

BBAR:

RHAL1_03314(inhA)

FIL:	BN1229_v1_1635(inhA)

FIY:	BN1229_v1_1638(inhA)

RHZ:	RHPLAN_36830

MCG:

GL4_0841

METG:

HT051_01605

DEQ:	XM25_09205(ThiJ)

DEI:	C4375_17025

DEVO:

H4N61_13280

YTI:	FNA67_12950

MBRY:

B1812_17210 B1812_17725

MHEY:

H2LOC_003240 H2LOC_011360

MPAR:

F7D14_14925 F7D14_17980

MTW:	CQW49_07215 CQW49_17635 CQW49_21200

CMET:

K6K41_10785

HDI:	HDIA_2813(inhA_1)

MEY:	TM49_15320

MAAD:

AZF01_11685

MMED:

Mame_03271(inhA)

MLUT:

JET14_12405

AUA:	M673_22285

PSIN:

CAK95_21510

NOH:	G5V57_17685 G5V57_23625

LABP:

FJ695_05355

PHR:	C6569_10245

PSTG:

E8M01_00590 E8M01_03780 E8M01_24970 E8M01_25820

ACUT:

MRB58_05880 MRB58_24380

CCR:	CC_1780 CC_2959

CCS:	CCNA_01858 CCNA_03055

CAK:

Caul_4318

CSE:

Cseg_3455

CHQ:	AQ619_14255

CMB:	CSW64_03465

CFH:	C1707_12810

CAUF:

CSW63_06645

CAUL:

KCG34_04730

PZU:

PHZ_c2614

PHB:	HYN04_01205 HYN04_09280

TSV:	DSM104635_02609(inhA)

TMD:	KUV46_11275

PARS:

DRW48_14065

SALO:

EF888_11095

BOO:	E2K80_00410

PPRU:

FDP22_01840

MMR:	Mmar10_2904

HNE:

HNE_1952

CBOT:

ATE48_03405

HBC:	AEM38_01845

NGF:	FRF71_02915

SAL:

Sala_0031

SPHK:

SKP52_03745

SPHP:

LH20_15765

SMAG:

AN936_06115

SMAZ:

LH19_15905

STER:

AOA14_04750

SGI:	SGRAN_1015(inhA)

SPHQ:

BWQ93_11985

SPHO:

C3E99_01450

SPHX:

E5675_16910

SPHU:

SPPYR_2980(inhA)

SPHI:

TS85_18445 TS85_20980

SKR:	BRX40_06675 BRX40_06760

SPLM:

BXU08_16230

SPHC:

CVN68_13070

SLUT:

H9L13_03915

SLAN:

GV829_01210

SDH:	H9L15_03960

SPAP:

H3Z74_22925

SPAI:

FPZ24_16095

SARI:

H5J25_14205

SCHY:

GVO57_02845 GVO57_10385

SAER:

NEF64_17665

SQO:	NMP03_11740

SCAB:

LZK98_02695

SRAD:

LLW23_15000

SAMI:

SAMIE_1007250 SAMIE_1016180 SAMIE_1016210

SPHG:

AZE99_01305

SFLA:

SPHFLASMR4Y_02170(inhA)

SPHY:

CHN51_04530

BLAS:

BSY18_1137

BFW:	B5J99_15045

PALG:

HFP57_17800

PARJ:

J4G78_00310 J4G78_11785 J4G78_16785 J4G78_16790

PHAO:

HF685_09895

SMIC:

SmB9_36710

SAND:

H3309_06925 H3309_07680

PMEA:

KTC28_02350

ADO:	A6F68_00187(inhA)

EMV:	HQR01_00910

GOH:

B932_3347

GBE:	GbCGDNIH1_1854

GBH:	GbCGDNIH2_1854

GBC:	GbCGDNIH3_1854

GBS:	GbCGDNIH4_1854

ACR:

Acry_0492

AMV:	ACMV_05670

RCV:	PFY06_18980

SRF:	LHU95_03515 LHU95_04580

RAP:	RHOA_1518(inhA)

ACIM:

GT370_17615 GT370_17640

AZL:	AZL_005750

ABS:	AZOBR_160005

ABQ:	ABAZ39_03600

ABF:	AMK58_10415

ATI:	AL072_18770

AHU:	A6A40_12435

AZT:	TSH58p_15135

AARE:

D3093_05620

NAO:	Y958_15830

NCB:	C0V82_11910

FER:	FNB15_18425

TMO:	TMO_0792 TMO_2584

THAC:

CSC3H3_01220 CSC3H3_22550 CSC3H3_22625

PBR:	PB2503_04287

HFL:	PUV54_03825

KOZ:	KFF44_07730

AFR:

AFE_2005

AFE:	Lferr_1672

ACU:

Atc_0156

AFI:	Acife_1491

AFJ:	AFERRID_06090

ACIY:

MQE22_13445

SFU:

Sfum_2623

SUR:	STAUR_6011

AGE:	AA314_03993

AVM:	JQX13_02040 JQX13_10415

MBD:	MEBOL_002351

CFUS:

CYFUS_000203 CYFUS_000268

SCL:	sce6836(thiJ) sce8831

SCU:	SCE1572_01610 SCE1572_01695

PAUU:

E8A73_029740 E8A73_031165

LLU:	AKJ09_02486

NANN:

O0S08_33200

MDX:	BTO20_34100

MSM:	MSMEG_3366

MSG:	MSMEI_3286

MSB:	LJ00_16745

MSN:	LI99_16750

MSH:	LI98_16755

MNE:	D174_02615

MYN:	MyAD_02550

MTY:	MTOK_31720

MMAT:

MMAGJ_17540

SSOI:

I1A49_00265

SAUO:

BV401_00505

CPHA:

FGI33_11060

CCAF:

FGD68_04360

MCW:	A8L33_04525

MBIN:

LXM64_09175

CUB:	BJK06_13445

CQF:	GBG65_01565

AGF:	ET445_04695

HEA:	HL652_00280

SUBA:

LQ955_03460

BGG:	CFK41_00385

BHH:	Bra3105_11470

OEK:	FFI11_011505

SAIU:

J4H86_00960

FAL:

FRAAL5358

KRA:

Krad_2391

APRE:

CNX65_21610

AMI:	Amir_3148 Amir_4315

ACTI:

UA75_06630

ACAD:

UA74_06630

AHG:	AHOG_06350(inhA1)

SYU:	M744_14170

SYF:	Synpcc7942_B2643

AMR:	AM1_3035 AM1_6311

GLP:	Glo7428_4360

CYP:	PCC8801_2269

CYH:	Cyan8802_2320

CYC:	PCC7424_3105

CYJ:	Cyan7822_0294

GVI:	gll0294 gll1979

GLJ:	GKIL_2120(ftrA)

GML:	ISF26_00405

ANA:

all7521

NON:	NOS3756_20200 NOS3756_47790

NOE:	CLI64_06080

AVA:

Ava_4332

ANN:	EH233_01905

RSIN:

B6N60_05260

CALN:

NIES2098_29350 NIES2098_32890

RIV:	Riv7116_3327

TOQ:	HCG51_22895

NCN:	BZZ01_19525

CTHE:

Chro_3529

RML:	FF011L_31840(inhA)

ROL:	CA51_22810(inhA)

AHEL:

Q31a_41550(inhA)

GES:	VT84_24845(inhA)

GMS:	SOIL9_31660

FTJ:

FTUN_0634

SACI:

Sinac_4134

AGV:	OJF2_67220(inhA)

LIL:

LA_0538

LIE:

LIF_A0444

LIC:

LIC_13026

LIS:

LIL_13132

LBJ:

LBJ_0469

LBL:

LBL_2610

LST:

LSS_00045

LAJ:	A0128_15935

LMAY:

DPV73_02790

LKM:	EFP84_07715

LWL:	FHG68_16175

LKB:	LPTSP3_g03310

LNO:	MAL08_02930

LKR:	FH602_03275

GGR:	HKW67_21185

GBA:

J421_1851

COY:	HF329_21835

CFIL:

MYF79_06910

ATEE:

K9M52_02820

HHY:	Halhy_2201

» show all

Reference

1 [PMID:11306561]

Authors

Goda M, Hashimoto Y, Shimizu S, Kobayashi M

Title

Discovery of a novel enzyme, isonitrile hydratase, involved in nitrogen-carbon triple bond cleavage.

Journal

J Biol Chem 276:23480-5 (2001)
DOI:10.1074/jbc.M007856200

Sequence

[ag:BAC41251]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.103

IUBMB Enzyme Nomenclature:

4.2.1.103

ExPASy - ENZYME nomenclature database:

4.2.1.103

UM-BBD (Biocatalysis/Biodegradation Database):

4.2.1.103

BRENDA, the Enzyme Database:

4.2.1.103

CAS:	358974-06-8

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