KEGG   ENZYME: 4.2.1.103
Entry
EC 4.2.1.103                Enzyme                                 
Name
cyclohexyl-isocyanide hydratase;
isonitrile hydratase;
N-cyclohexylformamide hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
N-cyclohexylformamide hydro-lyase (cyclohexyl-isocyanide-forming)
Reaction(IUBMB)
N-cyclohexylformamide = cyclohexyl isocyanide + H2O [RN:R05771]
Reaction(KEGG)
R05771
Substrate
N-cyclohexylformamide [CPD:C11519]
Product
cyclohexyl isocyanide [CPD:C11520];
H2O [CPD:C00001]
Comment
The enzyme from Pseudomonas putida strain N19-2 can also catalyse the hydration of other isonitriles to the corresponding N-substituted formamides. The enzyme has no metal requirements.
History
EC 4.2.1.103 created 2001
Pathway
ec00930  Caprolactam degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K18199  cyclohexyl-isocyanide hydratase
Genes
KPEKPK_B0025(inhA)
KGRJJJ10_22360
SYMK6K13_11445
SMARSM39_0772
SMACSMDB11_0595
SMWSMWW4_v1c13220
SRRSerAS9_1315
SRLSOD_c12300(inhA)
SRYM621_06870
SRSSerAS12_1315
SRASerAS13_1315
SLQM495_06150
SERFL085_21850
SERSSERRSCBI_06230
SERASer39006_016470
SERQCWC46_16470
SFJSAMEA4384070_1408
SSURATE40_003380
SURIJ0X03_17185
SFOZ042_02840
RAHRahaq_2239
RAQRahaq2_2344
RAAQ7S_11325
ROXBV494_12435
RVCJ9880_24115
GQUAWC35_05010
RBADH2866_07755
DDADd703_2109
LBQCKQ53_13760
LPOPI6N93_07775
SODSant_2439
PESSOPEG_2323
EBIEbC_00490
PAMPANA_1582
PLFPANA5342_2643
PAJPAJ_0920
PAQPAGR_g2559
PVAPvag_pPag30009
PAGGAL522_00945
PAOPat9b_5214
PANTPSNIH1_p00470
PAGCBEE12_21550
PANSFCN45_21235
PEYEE896_19710
PDISD8B20_19625
PERLAC65_16270
PAYPAU_02206
LPVHYN51_05745
PFQQQ39_04125
RGLCS053_12030
DTXATSB10_30330
PSTPSPTO_2847
PCIPCH70_32170
PCABJGS08_13025
PFLPFL_4109(inhA)
PPRCPFLCHA0_c41680
PPROPPC_4210
PFEPSF113_1826(inhA)
PFNHZ99_00150
PFOPfl01_1835
PFSPFLU_2085
PFCPflA506_1987(inhA)
PPZH045_06030
PFBVO64_1327
PMANOU5_1432
PTVAA957_04435
PCGAXG94_06590
PVRPverR02_10275
PAZOAYR47_17100
POIBOP93_09185
PRXHRH33_10535
PFWPF1751_v1c19050(inhA)
PFFPFLUOLIPICF727195
PFXA7318_08895
PLULFOB45_02625
PBAPSEBR_a3931
PBCCD58_20725
PPUUPputUW4_01540
PSKU771_10845
PCHEY04_20820
PCZPCL1606_20730
PCPJM49_10390
PSEMTO66_21510
PSOSPOS17_4209
PKRAYO71_12120
PFKPFAS1_02640
PANRA7J50_2095
PSILPMA3_20215
PYMAK972_1776
PKEDLD99_10010
PUMHGP31_22190
PGGFX982_03252
PGYAWU82_02140
PVKEPZ47_19870
PEZHWQ56_22285
PBZGN234_27555
PATAJWU58_18155
PZEHU754_010780
PMOEHV782_010360
PSEPC4K39_6042
PVWHU752_021385
PTRTHU722_0010610
PSAMHU731_025725
PTZHU718_010460
PQIKH389_07090
PSHHHU773_010815
PHVHU739_008405
PASGKSS96_10145
PAZEKSS91_08695
PALVKSS97_09660
PZAHU749_020645
PIEHU724_009930
SAVIJYB87_17060
MMAIsS8_4514
TSBHMY34_02050
KUYFY550_11460
ADIB5T_01317
AXEP40_06200
SALNSALB1_2907
CDIZCEDIAZO_00144(inhA)
CVICV_1313
CVCBKX93_19500
CHRIDK842_14120
CHRBDK843_19690
RSLRPSI07_mp0745
RSNRSPO_m01124
RPIRpic_3890
RPFRpic12D_4003
RINACS15_5592(inhA)
RSGJK151_21505
CNCCNE_BB1p06340 CNE_BB1p06350 CNE_BB1p06390
REUReut_C5977
CCUPBKK81_24965
CUPBKK80_31400
CUUBKK79_22285
COXE0W60_28170 E0W60_30800 E0W60_30825
BMABMAA0232
BMVBMASAVP1_1415
BMLBMA10229_1610
BMNBMA10247_A0269
BMALDM55_3487
BMAEDM78_4670
BMAQDM76_4184
BMAIDM57_14220
BMAFDM51_3982
BMAZBM44_3449
BMABBM45_4285
BPSBPSS1863
BPMBURPS1710b_A0954
BPLBURPS1106A_A2525
BPDBURPS668_A2666
BPSEBDL_5266
BPSMBBQ_4269
BPSUBBN_5326
BPSDBBX_4431
BPZBP1026B_II1996
BPQBPC006_II2486
BPKBBK_4598
BPSHDR55_5874
BPSABBU_4148
BPSOX996_5671
BUTX994_5235
BTEBTH_II0514
BTQBTQ_3807
BTJBTJ_4838
BTZBTL_5633
BTDBTI_5131
BTVBTHA_4577
BTHEBTN_5295
BTHMBTRA_5566
BTHADR62_5433
BTHLBG87_5640
BOKDM82_6139
BOCBG90_4526
BUUWS70_26755
BSAVWS86_28455
BVIBcep1808_2772
BVEAK36_1231
BURBcep18194_A5986 Bcep18194_C6949
BCNBcen_2047
BCHBcen2424_2658
BCMBcenmc03_2687
BCJBCAL0944
BCENDM39_2748
BCEWDM40_285
BCEOI35_2723
BAMBamb_2705
BACBamMC406_2579
BMJBMULJ_02622
BMUBmul_0639
BMKDM80_2260
BMULNP80_2794
BCTGEM_0778
BCEDDM42_2413
BCEPAPZ15_01180 APZ15_37075
BDLAK34_430
BPYRABD05_03325
BCONNL30_26365
BUBBW23_2234
BDFWI26_12850 WI26_18185
BLATWK25_12910
BTEIWS51_23710
BSEMWJ12_13325
BPSLWS57_32075
BMECWJ16_13640 WJ16_18360
BSTGWT74_14115
BSTLBBJ41_15470
BANNJFN94_13625 JFN94_18780
BGLbglu_1g11570
BGUKS03_2093
BGDbgla_1g13580 bgla_4p2640
BGOBM43_2691
BYIBYI23_B003790(pfpI)
BUKMYA_2423
BUEBRPE67_BCDS06360(pfpI)
BULBW21_4667
BGPBGL_1c12500
BPLAbpln_1g12010
BUDAQ610_29760
BUMAXG89_22605
BUIAX768_25090
BURKDM992_06690
BGEBC1002_3249
PSPWBJG93_19305
PARABTO02_25970
PMEGFNZ07_17295 FNZ07_18000
PTROG5S35_18420
PACPFAZ97_16555 FAZ97_22925
PACSFAZ98_21115
PEWKZJ38_28570
POXMB84_16600
PTXABW99_09470 ABW99_09765
PLGNCTC10937_01916
CABASBC2_66130(inhA_2)
BUOBRPE64_BCDS09480
BGMCAL15_04620 CAL15_16450
AXYAXYL_02520
AXONH44784_041151
AXNAX27061_2302
AXXERS451415_02297
ADTAPT56_11265
AISBUW96_02845
ASWCVS48_15890
ACHRC2U31_02555 C2U31_23205
ACHBDVB37_09800
ADYHLG70_11255
AMIMMIM_c36260
ODIODI_R3652
PACRFXN63_14005
AAVAave_3882
LIHL63ED372_02726(inhA)
PBHAAW51_3464
CFUCFU_1980
CPRACPter91_1266
MNRACZ75_09635
MIUABE85_09740
SURSTAUR_6011
AGEAA314_03993
AVMJQX13_02040 JQX13_10415
MBDMEBOL_002351
CFUSCYFUS_000203 CYFUS_000268
SCLsce6836(thiJ) sce8831
SCUSCE1572_01610 SCE1572_01695
PAUUE8A73_029740 E8A73_031165
LLUAKJ09_02486
SFUSfum_2623
MAMOA6B35_06360
MESPC1M53_09140 C1M53_24005
PHTBLM14_21445
PMOBHG718_06600
SFHSFHH103_06122
ARAArad_8694
ATFAch5_50350
AVIAvi_1644 Avi_7628
AGTEYD00_13990 EYD00_23945
ARUIG6M88_05190
RTRRTCIAT899_PC03145
RJGCCGE525_28305
RHRCKA34_22720
RLSHB780_08580
NGLRG1141_CH10730
NGGRG540_CH10970
NENNCHU2750_51300
KAIK32_01220 K32_14080
OINIAR37_11580
BJAbll4280(bll4280)
BJUBJ6T_55320
BJPRN69_26815
BRABRADO3481 BRADO4159
BBTBBta_4230 BBta_4536
BRSS23_30320 S23_40040 S23_56940
AOLS58_36400
BRCBCCGELA001_07170 BCCGELA001_17850
BRADBF49_0765
BICLMTR13_21705
BROBRAD285_3854
BRKCWS35_00885 CWS35_16175 CWS35_22960 CWS35_35285
BOTCIT37_28250 CIT37_32700
BRQCIT40_14000 CIT40_18185
BGQX265_17920
BGZXH91_19540 XH91_27890
BSYMCIT39_13170 CIT39_17535
BBETF8237_00370
BARHWN72_23560
BVZBRAD3257_4752(inhA)
BELBE61_60030 BE61_85190
BDGLPJ38_23320
BSEPHAP48_0021345 HAP48_0036700 HAP48_0044390
BQBJ4P68_0012710 J4P68_0037910
RPARPA2422
RPBRPB_2555 RPB_3031
RPCRPC_2887
RPTRpal_2700
RPXRpdx1_3085
RBKE0H22_15260
OCAOCAR_6811
OCGOCA5_c12650
OCOOCA4_c12650
VGOGJW-30_1_00522(inhA_1)
TRBHB776_04255
BVVBHK69_11790
BOIBLM15_04605 BLM15_18455
BOFFQV39_04355
BAPIBBC0122_024120
XAUXaut_1973
XDIEZH22_04670
AZCAZC_2741
LNEFZC33_17880
MEAMex_1p0926(inh) Mex_1p3533(inh) Mex_1p4818(inh)
MDIMETDI1727(inh) METDI4110(inh) METDI5414(inh)
MEXMext_1154 Mext_3318 Mext_4368
MCHMchl_1313 Mchl_3642 Mchl_4844
MPOMpop_3518
MZAB2G69_03875 B2G69_13040 B2G69_24145
MRDMrad2831_1653 Mrad2831_3391
METM446_4875
MNOMnod_1849
MORMOC_2046 MOC_3899
METAY590_04890 Y590_16250
MAQUMaq22A_c27365(thiJ)
MPHYMCBMB27_01301 MCBMB27_02881
MEEDA075_03875 DA075_33305 DA075_33345
METDC0214_18295 C0214_24205
METXA3862_14490 A3862_22610
METSDK389_08825 DK389_09000 DK389_09835 DK389_27220
METIDK427_05315 DK427_16925
MMESMMSR116_01165 MMSR116_12260
MTEADK419_05255
MINDmvi_12520 mvi_37970
MOGMMB17_16905
BIDBind_1474
MLGCWB41_14540
BBARRHAL1_03314(inhA)
FILBN1229_v1_1635(inhA)
FIYBN1229_v1_1638(inhA)
RHZRHPLAN_36830
MCGGL4_0841
METGHT051_01605
DEQXM25_09205(ThiJ)
DEIC4375_17025
YTIFNA67_12950
MBRYB1812_17210 B1812_17725
MHEYH2LOC_003240 H2LOC_011360
MPARF7D14_14925 F7D14_17980
MTWCQW49_07215 CQW49_17635 CQW49_21200
CMETK6K41_10785
HDIHDIA_2813(inhA_1)
MEYTM49_15320
MAADAZF01_11685
MMEDMame_03271(inhA)
MLUTJET14_12405
AUAM673_22285
PSINCAK95_21510
NOHG5V57_17685 G5V57_23625
LABPFJ695_05355
PHRC6569_10245
PSTGE8M01_00590 E8M01_03780 E8M01_24970 E8M01_25820
ACUTMRB58_05880 MRB58_24380
CCRCC_1780 CC_2959
CCSCCNA_01858 CCNA_03055
CAKCaul_4318
CSECseg_3455
CHQAQ619_14255
CMBCSW64_03465
CFHC1707_12810
CAUFCSW63_06645
CAULKCG34_04730
PZUPHZ_c2614
PHBHYN04_01205 HYN04_09280
TSVDSM104635_02609(inhA)
TMDKUV46_11275
PARSDRW48_14065
SALOEF888_11095
BOOE2K80_00410
PPRUFDP22_01840
MMRMmar10_2904
HNEHNE_1952
CBOTATE48_03405
HBCAEM38_01845
NGFFRF71_02915
SALSala_0031
SPHKSKP52_03745
SPHPLH20_15765
SMAGAN936_06115
SMAZLH19_15905
STERAOA14_04750
SGISGRAN_1015(inhA)
SPHQBWQ93_11985
SPHOC3E99_01450
SPHXE5675_16910
SPHUSPPYR_2980(inhA)
SPHITS85_18445 TS85_20980
SKRBRX40_06675 BRX40_06760
SPLMBXU08_16230
SPHCCVN68_13070
SLUTH9L13_03915
SLANGV829_01210
SDHH9L15_03960
SPAPH3Z74_22925
SPAIFPZ24_16095
SARIH5J25_14205
SCHYGVO57_02845 GVO57_10385
SAMISAMIE_1007250 SAMIE_1016180 SAMIE_1016210
SPHGAZE99_01305
SFLASPHFLASMR4Y_02170(inhA)
SPHYCHN51_04530
BLASBSY18_1137
BFWB5J99_15045
PALGHFP57_17800
PARJJ4G78_00310 J4G78_11785 J4G78_16785 J4G78_16790
PHAOHF685_09895
SMICSmB9_36710
SANDH3309_06925 H3309_07680
ADOA6F68_00187(inhA)
EMVHQR01_00910
GOHB932_3347
GBEGbCGDNIH1_1854
GBHGbCGDNIH2_1854
GBCGbCGDNIH3_1854
GBSGbCGDNIH4_1854
ACRAcry_0492
AMVACMV_05670
AZLAZL_005750
ABSAZOBR_160005
ABQABAZ39_03600
ABFAMK58_10415
ATIAL072_18770
AHUA6A40_12435
AZTTSH58p_15135
NAOY958_15830
NCBC0V82_11910
TMOTMO_0792 TMO_2584
FERFNB15_18425
THACCSC3H3_01220 CSC3H3_22550 CSC3H3_22625
PBRPB2503_04287
AFRAFE_2005
AFELferr_1672
ACUAtc_0156
AFIAcife_1491
AFJAFERRID_06090
MDXBTO20_34100
MSMMSMEG_3366
MSGMSMEI_3286
MSBLJ00_16745
MSNLI99_16750
MSHLI98_16755
MNED174_02615
MYNMyAD_02550
MTYMTOK_31720
MMATMMAGJ_17540
SSOII1A49_00265
SAUOBV401_00505
MCWA8L33_04525
CUBBJK06_13445
CQFGBG65_01565
AGFET445_04695
HEAHL652_00280
BGGCFK41_00385
OEKFFI11_011505
SAIUJ4H86_00960
FALFRAAL5358
KRAKrad_2391
APRECNX65_21610
AMIAmir_3148 Amir_4315
ACTIUA75_06630
ACADUA74_06630
AHGAHOG_06350(inhA1)
SYUM744_14170
SYFSynpcc7942_B2643
AMRAM1_3035 AM1_6311
GLPGlo7428_4360
CYPPCC8801_2269
CYHCyan8802_2320
CYCPCC7424_3105
CYJCyan7822_0294
GVIgll0294 gll1979
GLJGKIL_2120(ftrA)
GMLISF26_00405
ANAall7521
NONNOS3756_20200 NOS3756_47790
NOECLI64_06080
AVAAva_4332
ANNEH233_01905
RSINB6N60_05260
RIVRiv7116_3327
TOQHCG51_22895
NCNBZZ01_19525
CTHEChro_3529
RMLFF011L_31840(inhA)
ROLCA51_22810(inhA)
AHELQ31a_41550(inhA)
GESVT84_24845(inhA)
GMSSOIL9_31660
FTJFTUN_0634
SACISinac_4134
AGVOJF2_67220(inhA)
LILLA_0538
LIELIF_A0444
LICLIC_13026
LISLIL_13132
LBJLBJ_0469
LBLLBL_2610
LSTLSS_00045
LAJA0128_15935
LMAYDPV73_02790
LKMEFP84_07715
LWLFHG68_16175
LKBLPTSP3_g03310
GGRHKW67_21185
GBAJ421_1851
COYHF329_21835
HHYHalhy_2201
 » show all
Reference
1  [PMID:11306561]
  Authors
Goda M, Hashimoto Y, Shimizu S, Kobayashi M
  Title
Discovery of a novel enzyme, isonitrile hydratase, involved in nitrogen-carbon triple bond cleavage.
  Journal
J Biol Chem 276:23480-5 (2001)
DOI:10.1074/jbc.M007856200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.103
IUBMB Enzyme Nomenclature: 4.2.1.103
ExPASy - ENZYME nomenclature database: 4.2.1.103
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.103
BRENDA, the Enzyme Database: 4.2.1.103
CAS: 358974-06-8

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