KEGG   ENZYME: 4.2.1.107
Entry
EC 4.2.1.107                Enzyme                                 
Name
3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase;
46 kDa hydratase 2;
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase [(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA-forming]
Reaction(IUBMB)
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O [RN:R04813]
Reaction(KEGG)
R04813;
(other) R04809
Substrate
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA [CPD:C15614]
Product
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA [CPD:C05460];
H2O [CPD:C00001]
Comment
This enzyme forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. The enzyme is involved in the beta-oxidation of the cholesterol side chain in the cholic-acid-biosynthesis pathway.
History
EC 4.2.1.107 created 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec01100  Metabolic pathways
Orthology
K12405  (3R)-3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2
Genes
HSA: 3295(HSD17B4)
PTR: 462019(HSD17B4)
PPS: 100981028(HSD17B4)
GGO: 101129538(HSD17B4)
PON: 100448539(HSD17B4)
NLE: 100590035(HSD17B4)
HMH: 116470899(HSD17B4)
MCC: 698369(HSD17B4)
MCF: 102123675(HSD17B4)
MTHB: 126957048
MNI: 105500031(HSD17B4)
CSAB: 103244395(HSD17B4)
CATY: 105590649(HSD17B4)
PANU: 101004327(HSD17B4)
TGE: 112625827(HSD17B4)
MLEU: 105534163(HSD17B4)
RRO: 104654581(HSD17B4)
RBB: 108529825(HSD17B4)
TFN: 117065971(HSD17B4)
PTEH: 111530208(HSD17B4)
CANG: 105527387(HSD17B4)
CJC: 100390100(HSD17B4)
SBQ: 101036776(HSD17B4)
CIMI: 108306790(HSD17B4)
CSYR: 103262046
MMUR: 105872240
LCAT: 123648404(HSD17B4)
PCOQ: 105822547(HSD17B4)
OGA: 100966879(HSD17B4)
MMU: 15488(Hsd17b4)
MCAL: 110284447(Hsd17b4)
MPAH: 110332881(Hsd17b4)
RNO: 79244(Hsd17b4)
MCOC: 116087150(Hsd17b4)
ANU: 117719952(Hsd17b4)
CGE: 103161104(Hsd17b4)
MAUA: 101827399(Hsd17b4)
PROB: 127234029(Hsd17b4)
PLEU: 114694768(Hsd17b4)
MORG: 121441957 121453615(Hsd17b4)
MFOT: 126505968
AAMP: 119815878(Hsd17b4)
NGI: 103745113(Hsd17b4)
HGL: 101713520(Hsd17b4)
CPOC: 100379226(Hsd17b4)
CCAN: 109674145
DORD: 105985471(Hsd17b4)
DSP: 122101973(Hsd17b4)
NCAR: 124986751
OCU: 100346781
OPI: 101530287(HSD17B4)
TUP: 102468828(HSD17B4)
GVR: 103605910(HSD17B4)
CFA: 474630(HSD17B4)
CLUD: 112659672(HSD17B4)
VVP: 112932403(HSD17B4)
VLG: 121494637(HSD17B4)
AML: 100471749(HSD17B4)
UMR: 103670554(HSD17B4)
UAH: 113261629(HSD17B4)
UAR: 123790430(HSD17B4)
ELK: 111158891
LLV: 125100000
MPUF: 101683185(HSD17B4)
NVS: 122916269(HSD17B4)
ORO: 101385731(HSD17B4)
EJU: 114209435(HSD17B4)
ZCA: 113928790(HSD17B4)
MLX: 118016560(HSD17B4)
NSU: 110590913(HSD17B4)
LWW: 102736737(HSD17B4)
FCA: 101091840(HSD17B4)
PYU: 121010581(HSD17B4)
PCOO: 112861741(HSD17B4)
PBG: 122486641(HSD17B4)
LRUF: 124515159
PTG: 102968873(HSD17B4)
PPAD: 109278892(HSD17B4)
PUC: 125934835
AJU: 106975089(HSD17B4)
HHV: 120240242(HSD17B4)
BTA: 493643(HSD17B4)
BOM: 102268517(HSD17B4)
BBUB: 102412917(HSD17B4)
BBIS: 104993366(HSD17B4)
CHX: 102172142(HSD17B4)
OAS: 101114519(HSD17B4)
ODA: 120864671(HSD17B4)
CCAD: 122440074(HSD17B4)
SSC: 397574(HSD17B4)
CFR: 102508317(HSD17B4)
CBAI: 105082053(HSD17B4)
CDK: 105104293(HSD17B4)
VPC: 102537534(HSD17B4)
LVE: 103084787(HSD17B4)
OOR: 101282963(HSD17B4)
DLE: 111183342(HSD17B4)
PCAD: 102996127(HSD17B4)
PSIU: 116751686(HSD17B4)
NASI: 112398657(HSD17B4)
ECB: 100034046(HSD17B4)
EPZ: 103548315(HSD17B4)
EAI: 106833039(HSD17B4)
MYB: 102240475(HSD17B4)
MYD: 102767159(HSD17B4)
MMYO: 118656167(HSD17B4)
MLF: 102425700(HSD17B4)
MNA: 107530713(HSD17B4)
PKL: 118703106(HSD17B4)
HAI: 109374101(HSD17B4)
DRO: 112315031(HSD17B4)
SHON: 118975196(HSD17B4)
AJM: 119051192(HSD17B4)
PDIC: 114494306(HSD17B4)
PHAS: 123818113(HSD17B4)
MMF: 118642891(HSD17B4)
RFQ: 117024890(HSD17B4)
PALE: 102887000(HSD17B4)
PGIG: 120590862(HSD17B4)
PVP: 105290860(HSD17B4)
RAY: 107511154(HSD17B4)
TOD: 119243559(HSD17B4)
SARA: 101542842(HSD17B4)
LAV: 100659299(HSD17B4)
TMU: 101356123
ETF: 101641151(HSD17B4) 101644420
DNM: 101423884(HSD17B4)
MDO: 100028098(HSD17B4)
GAS: 123235791(HSD17B4)
SHR: 100932771(HSD17B4)
PCW: 110202743(HSD17B4)
OAA: 100081754(HSD17B4)
GGA: 395785(HSD17B4)
PCOC: 116234067(HSD17B4)
CJO: 107306670(HSD17B4)
NMEL: 110389447(HSD17B4)
APLA: 101791118(HSD17B4)
ACYG: 106048362(HSD17B4)
AFUL: 116500754(HSD17B4)
TGU: 100218226(HSD17B4)
LSR: 110479177(HSD17B4)
SCAN: 103821465(HSD17B4)
PMOA: 120512524(HSD17B4)
OTC: 121332706(HSD17B4)
PRUF: 121362562(HSD17B4)
GFR: 102036502(HSD17B4)
FAB: 101807233(HSD17B4)
PHI: 102109547(HSD17B4)
PMAJ: 107216347(HSD17B4)
CCAE: 111941198(HSD17B4)
CCW: 104690497(HSD17B4)
CBRC: 103611629(HSD17B4)
ETL: 114056305(HSD17B4)
ZAB: 102062238(HSD17B4)
ACHL: 103806159(HSD17B4)
SVG: 106851983(HSD17B4)
FPG: 101917400(HSD17B4)
FCH: 102058406(HSD17B4)
CLV: 102095771(HSD17B4)
EGZ: 104122491(HSD17B4)
NNI: 104014571(HSD17B4)
PLET: 104618021(HSD17B4)
ACUN: 113489653(HSD17B4)
TALA: 104363287(HSD17B4)
PADL: 103912347(HSD17B4)
AFOR: 103897966(HSD17B4)
ACHC: 115336530(HSD17B4)
HALD: 104320961(HSD17B4)
HLE: 104828588(HSD17B4)
AGEN: 126036470
GCL: 127027587
CSTI: 104557391(HSD17B4)
CMAC: 104480460(HSD17B4)
MUI: 104541285(HSD17B4)
FGA: 104069667(HSD17B4)
LDI: 104348760(HSD17B4)
OHA: 104339186(HSD17B4)
NNT: 104407165(HSD17B4)
SHAB: 115619609(HSD17B4)
DPUB: 104304758(HSD17B4)
PGUU: 104458725(HSD17B4)
CPEA: 104392915(HSD17B4)
AVIT: 104272741(HSD17B4)
CVF: 104294213(HSD17B4)
CUCA: 104061043(HSD17B4)
BRHI: 104495883(HSD17B4)
AROW: 112978041(HSD17B4)
NPD: 112958912(HSD17B4)
TGT: 104571515(HSD17B4)
DNE: 112992405
SCAM: 104150534(HSD17B4)
ASN: 102371766(HSD17B4)
AMJ: 102571005(HSD17B4)
CPOO: 109323436(HSD17B4)
GGN: 109297318(HSD17B4)
PSS: 102451304(HSD17B4)
CMY: 102933522(HSD17B4)
CPIC: 101945395(HSD17B4)
TST: 117879364(HSD17B4)
CABI: 116836212(HSD17B4)
MRV: 120408024(HSD17B4)
ACS: 100558484(hsd17b4)
PVT: 110084344(HSD17B4)
SUND: 121921111(HSD17B4)
PBI: 103063870(HSD17B4)
PMUR: 107294349(HSD17B4)
CTIG: 120316078(HSD17B4)
TSR: 106543382(HSD17B4)
PGUT: 117674677(HSD17B4)
VKO: 123031365(HSD17B4)
PMUA: 114606393(HSD17B4)
ZVI: 118076708(HSD17B4)
HCG: 128345823(HSD17B4)
GJA: 107116225(HSD17B4)
STOW: 125436558(HSD17B4)
XLA: 444492(hsd17b4.L)
XTR: 613082(hsd17b4)
NPR: 108787696(HSD17B4)
RTEM: 120931986(HSD17B4)
BBUF: 120992600(HSD17B4)
BGAR: 122924617(HSD17B4)
DRE: 393105(hsd17b4)
SANH: 107703629(hsd17b4)
SGH: 107571774(hsd17b4)
CAUA: 113094195 113106903
CGIB: 127963370
PPRM: 120475505(hsd17b4)
MAMB: 125279588(hsd17b4)
CIDE: 127517328
MASI: 127414554 127423847
IPU: 108265438(hsd17b4)
PHYP: 113529268(hsd17b4)
SMEO: 124399595(hsd17b4)
TFD: 113656214(hsd17b4)
AMEX: 103046836(hsd17b4)
EEE: 113571049(hsd17b4)
TRU: 101062498(hsd17b4)
TNG: GSTEN00030945G001
LCO: 104920820(hsd17b4)
NCC: 104966339(hsd17b4)
CGOB: 115013548(hsd17b4)
ELY: 117252434(hsd17b4)
EFO: 125890843(hsd17b4)
PLEP: 121944362(hsd17b4)
SLUC: 116054060(hsd17b4)
ECRA: 117944965(hsd17b4)
ESP: 116689590(hsd17b4)
PFLV: 114555481(hsd17b4)
GAT: 120830783(hsd17b4)
PPUG: 119225552(hsd17b4)
MSAM: 119891683(hsd17b4)
CUD: 121509730(hsd17b4)
ALAT: 119019193(hsd17b4)
MZE: 101482787(hsd17b4)
ONL: 100706762(hsd17b4)
OAU: 116310500(hsd17b4)
OLA: 101159921(hsd17b4)
OML: 112148954(hsd17b4)
XMA: 102223774(hsd17b4)
XCO: 114154573(hsd17b4)
XHE: 116730397(hsd17b4)
PRET: 103470095(hsd17b4)
PFOR: 103141760(hsd17b4)
PLAI: 106944940(hsd17b4)
PMEI: 106923064(hsd17b4)
GAF: 122827419(hsd17b4)
CVG: 107086311(hsd17b4)
CTUL: 119798579(hsd17b4)
GMU: 124877861(hsd17b4)
NFU: 107394149(hsd17b4)
KMR: 108233704(hsd17b4)
ALIM: 106513677(hsd17b4)
NWH: 119413904(hsd17b4)
AOCE: 111564685(hsd17b4)
MCEP: 125012708(hsd17b4)
POV: 109637138(hsd17b4)
SSEN: 122769476(hsd17b4)
HHIP: 117768550(hsd17b4)
HSP: 118114529(hsd17b4)
LCF: 108882142
SDU: 111230866(hsd17b4)
SLAL: 111645149(hsd17b4)
XGL: 120805559(hsd17b4)
HCQ: 109526033(hsd17b4)
SSCV: 125993727
BPEC: 110167024(hsd17b4)
MALB: 109956607(hsd17b4)
BSPL: 114861822(hsd17b4)
SASA: 106585849(hsd17b4)
STRU: 115164957(hsd17b4)
OTW: 112247069(hsd17b4)
OMY: 110525264(hsd17b4)
OGO: 124034633(hsd17b4)
ONE: 115111275(hsd17b4)
SALP: 111974039(hsd17b4)
SNH: 120045172(hsd17b4)
CCLU: 121584411(hsd17b4)
ELS: 105014609(hsd17b4)
SFM: 108938133(hsd17b4)
PKI: 111856620(hsd17b4)
AANG: 118206736(hsd17b4)
LOC: 102697531(hsd17b4)
PSPA: 121302596(hsd17b4) 121316706
ARUT: 117427495 117973220
PSEX: 120532694(hsd17b4)
LCM: 102356548(HSD17B4)
CMK: 103181119(hsd17b4)
CPLA: 122560645(hsd17b4)
BFO: 118409354
BBEL: 109480804
CIN: 100187421
SCLV: 120338122
SPU: 581580
AJC: 117122621
DME: Dmel_CG3415(Mfe2)
DPO: 4815128
DMN: 108161558
DGR: 6565453
RZE: 108381954
AGA: AgaP_AGAP008667
ACOZ: 120955687
AARA: 120901803
AMER: 121597830
ASTE: 118512713
AAG: 5568633
AALB: 109428196
CQU: CpipJ_CPIJ001576
CPII: 120414661
CNS: 116337519 116349688
BCOO: 119079223 119084844
NVI: 100123018
CGLO: 123268667
FAS: 105272013
DAM: 107040917
AGIF: 122847409
CINS: 118067462
TCA: 663655(17-beta-HD4)
SOY: 115888404
CSET: 123306476
AGB: 108915760
LDC: 111505860
APLN: 108736403
PPYR: 116158989 116159225 116159796 116159821 116176974 116179454
OTU: 111423793
BMOR: 101745499
PBX: 123713006
PNAP: 125062929
CCRC: 123699116
CFEL: 113389004
API: 100167575
DNX: 107163428
AGS: 114120233
RMD: 113555196
DCI: 103506752 108253294
HHAL: 106683228
NLU: 111057072
FOC: 113212460
TPAL: 117645519
CSEC: 111873091
SGRE: 126271985 126315400 126317089
DPX: DAPPUDRAFT_332860
DMK: 116931070
DPZ: 124349493
PVM: 113818099
PJA: 122257684
PCHN: 125025607
PMOO: 119577269
HAME: 121875772
PCLA: 123765087
PTRU: 123518804
ESN: 126999011
HAZT: 108681612
EAF: 111696524
LSM: 121129872
PPOI: 119094250 119094251
DSV: 119440587
RSAN: 119387719
VDE: 111252755
VJA: 111266535
DFR: 124493442 124496269
SDM: 118180496
CEL: CELE_E04F6.3(maoc-1) CELE_M03A8.1(dhs-28)
CBR: CBG_10899(Cbr-dhs-28)
BGT: 106065027
GAE: 121385566
HRF: 124143730
HRJ: 124264477
CRG: 105326295
MYI: 110453744
PMAX: 117334218
MCAF: 127733788
MMER: 123566556
DPOL: 127875952
OSN: 115209823
NVE: 5504006
ATEN: 116294223
XEN: 124434950
TAD: TRIADDRAFT_19721
MBR: MONBRDRAFT_23152
DDI: DDB_G0291247(mfeA)
TET: TTHERM_00317440
PCAY: FRD00_09620
 » show all
Reference
1  [PMID:9371691]
  Authors
Qin YM, Haapalainen AM, Conry D, Cuebas DA, Hiltunen JK, Novikov DK.
  Title
Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis.
  Journal
Biochem J 328 ( Pt 2):377-82 (1997)
DOI:10.1042/bj3280377
Reference
2  [PMID:8619845]
  Authors
Xu R, Cuebas DA.
  Title
The reactions catalyzed by the inducible bifunctional enzyme of rat liver peroxisomes cannot lead to the formation of bile acids.
  Journal
Biochem Biophys Res Commun 221:271-8 (1996)
DOI:10.1006/bbrc.1996.0585
Reference
3
  Authors
Kinoshita, T., Miyata, M., Ismail, S.M., Fujimoto, Y., Kakinuma, K., Kokawa, N.I. and Morisaki, M.
  Title
Synthesis and determination of stereochemistry of four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem Pharm Bull 36:134-141 (1988)
Reference
4
  Authors
Fujimoto, Y., Kinoshita, T., Oya, I., Kakinuma, K., Ismail, S.M., Sonoda, Y., Sato, Y. and Morisaki, M.
  Title
Non-stereoselective conversion of the four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem Pharm Bull 36:142-145 (1988)
Reference
5  [PMID:11146090]
  Authors
Kurosawa T, Sato M, Nakano H, Fujiwara M, Murai T, Yoshimura T, Hashimoto T.
  Title
Conjugation reactions catalyzed by bifunctional proteins related to beta-oxidation in bile acid biosynthesis.
  Journal
Steroids 66:107-14 (2001)
DOI:10.1016/S0039-128X(00)00217-8
Reference
6  [PMID:12543708]
  Authors
Russell DW
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.107
IUBMB Enzyme Nomenclature: 4.2.1.107
ExPASy - ENZYME nomenclature database: 4.2.1.107
BRENDA, the Enzyme Database: 4.2.1.107
CAS: 152787-68-3

  All links  
Pathway (7)   
   KEGG PATHWAY (4)   
   KEGG MODULE (3)   
Chemical substance (5)   
   KEGG COMPOUND (5)   
Chemical reaction (3)   
   KEGG REACTION (2)   
   KEGG RCLASS (1)   
Gene (7198)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (421)   
   KEGG MGENES (471)   
   RefGene (6305)   
Protein sequence (155)   
   UniProt (134)   
   SWISS-PROT (3)   
   RefSeq(pep) (18)   
DNA sequence (139)   
   RefSeq(nuc) (23)   
   GenBank (64)   
   EMBL (52)   
Protein domain (7)   
   InterPro (7)   
All databases (7514)   

Download RDF
DBGET integrated database retrieval system