KEGG ENZYME: 4.2.1.135

ENZYME: 4.2.1.135

Entry

EC 4.2.1.135 Enzyme

Name

UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining);
PglF

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

UDP-N-acetyl-alpha-D-glucosamine hydro-lyase (configuration-retaining; UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose-forming)

Reaction(IUBMB)

UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O [RN:R10266]

Reaction(KEGG)

R10266

Substrate

UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043]

Product

UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose [CPD:C04613];
H2O [CPD:C00001]

Comment

Contains NAD+ as a cofactor [2]. This is the first enzyme in the biosynthetic pathway of N,N'-diacetylbacillosamine [1], the first carbohydrate in the glycoprotein N-linked heptasaccharide in Campylobacter jejuni. This enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes.

History

EC 4.2.1.135 created 2012

Pathway

ec00520

Amino sugar and nucleotide sugar metabolism

ec01100

Metabolic pathways

Orthology

K15912

UDP-N-acetyl-D-glucosamine 4,6-dehydratase

Genes

FTQ:

RO31_1719

FTR:	NE061598_08175

FTT:	FTV_1398(wbtA)

FTG:	FTU_1482(wbtA)

FTS:

F92_03230

FTI:	FTS_0591(wbtA)

FTO:	X557_03145

FTC:

FTV:

FTZ:

FTX:

FTD:

FTY:

FCF:	FNFX1_1468

FCN:	FN3523_1497

FHI:	FSC454_07385

FPT:

FPI:

FPM:

FPX:

FPZ:

FPJ:

FNA:

FNL:	M973_08345

FRF:	LO80_08495

FPER:

ACH24_00765

FHA:	CDV26_09175

FRX:	F7310_02795

FRM:	BBG19_0349

FRC:

KX01_1765

FAD:	CDH04_02665

FMI:	F0R74_06090

FOO:	CGC45_01745

FGU:	SD28_06795

WSU:	WS0036(PGLF)

SULG:

FJR48_03875

SKU:	Sulku_2414

CJE:	Cj1120c(pglF)

CJB:	BN148_1120c(pglF)

CJJ:	CJJ81176_1138(pglF)

CJU:	C8J_1061(wlaL)

CJN:	ICDCCJ07001_1076

CJI:	CJSA_1062(pglF)

CJM:	CJM1_1099(pglF)

CJS:

CJS3_1167

CJP:	A911_05425

CJEJ:

CJEU:

CJEN:

CJEI:

CJER:

CJV:	MTVDSCj20_1124(pglF)

CJY:	QZ67_01197(pglF)

CJQ:	UC78_1078(pglF)

CJL:	PJ17_05745

CJW:	PJ18_05555

CJR:	CJE1263(pglF)

CJZ:	M635_01305

CJX:	BN867_11120

CFF:	CFF8240_1354

CFT:	CFF04554_1370(pglF)

CFV:	CFVI03293_1393(pglF)

CFX:	CFV97608_1493(pglF)

CFZ:

CSG_14930

CAMP:

CFT03427_1318(pglF)

CFP:	CR44_06670

CCV:	CCV52592_1202(pglF)

CHA:	CHAB381_0760 CHAB381_0949

CCO:	CCC13826_0447(pglF)

CCOC:

CCON33237_1460(pglF)

CLA:	CLA_1258(pglF)

CLR:	UPTC16701_1241(pglF)

CLM:	UPTC16712_1259(pglF)

CLQ:	UPTC4110_1243(pglF)

CLN:	UPTC3659_1478(pglF)

CLL:	CONCH_1209(pglF)

CCOL:

BN865_10080

CCC:	G157_03330

CCQ:	N149_1062(pglF)

CCF:

YSQ_03365

CCY:

YSS_06070

CCOI:

YSU_03400

CCOF:

VC76_05500(pglF)

CCOO:

ATE51_01362(pglF)

CAJ:	CIG1485E_1365(pglF)

CIS:	CINS_1223(pglF)

CVO:	CVOL_1237(pglF)

CPEL:

CPEL_1364(pglF)

CAMR:

CAQ16704_1269(pglF)

CSM:	CSUB8521_1445(pglF)

CSF:	CSUB8523_1541(pglF)

CGRA:

CGRAC_1485(pglF)

CURE:

CUREO_1368(pglF)

CHYO:

CHH_1359(pglF)

CHV:	CHELV3228_0575(pglF)

CSPF:

CSF_1366(pglF)

CPIN:

CPIN18020_1228(pglF)

CCUN:

CCUN_1176(pglF)

CLX:	CLAN_1155(pglF)

CAVI:

CAV_0411(pglF)

CHW:	A2J15_001375

CAMZ:

CVIC12175_0407(pglF)

CAMY:

CSUIS_1213(pglF)

ABT:

ABED_0646

ABL:	A7H1H_0682(pglF)

ASK:	EI285_05325(pglF)

AHS:	AHALO_0836

AMYT:

AMAR:

ATP:

ACAA:

AELL:

HBV:

ARC:

SBA:	Sulba_1854

SMUL:

SMUL_2465

SHAL:

SHALO_2211

SULT:

FA592_03745(pglF)

SLH:	YH65_07055

CPAF:

C6V80_08590

» show all

Reference

1 [PMID:16286454]

Authors

Schoenhofen IC, McNally DJ, Vinogradov E, Whitfield D, Young NM, Dick S, Wakarchuk WW, Brisson JR, Logan SM

Title

Functional characterization of dehydratase/aminotransferase pairs from Helicobacter and Campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways.

Journal

J Biol Chem 281:723-32 (2006)
DOI:10.1074/jbc.M511021200

Sequence

[cje:Cj1120c]

Reference

2 [PMID:17087520]

Authors

Olivier NB, Chen MM, Behr JR, Imperiali B

Title

In vitro biosynthesis of UDP-N,N'-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system.

Journal

Biochemistry 45:13659-69 (2006)
DOI:10.1021/bi061456h