KEGG ENZYME: 4.2.1.147

ENZYME: 4.2.1.147

Entry

EC 4.2.1.147                Enzyme

Name

5,6,7,8-tetrahydromethanopterin hydro-lyase;
formaldehyde-activating enzyme

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

5,6,7,8-tetrahydromethanopterin hydro-lyase (formaldehyde-adding, tetrahydromethanopterin-forming)

Reaction(IUBMB)

5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O [RN:R08058]

Reaction(KEGG)

R08058

Substrate

5,6,7,8-tetrahydromethanopterin [CPD:C01217];
formaldehyde [CPD:C00067]

Product

5,10-methylenetetrahydromethanopterin [CPD:C04377];
H2O [CPD:C00001]

Comment

Found in methylotrophic bacteria and methanogenic archaea.

History

EC 4.2.1.147 created 2014

Pathway

ec00680

Methane metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K10713

5,6,7,8-tetrahydromethanopterin hydro-lyase

K13812

bifunctional enzyme Fae/Hps

Genes

KMI:	VW41_19435

CNT:	JT31_16265

CEM:	LH23_01445

CEN:	LH86_01435

YPE:

YPO2460

YPK:

y1729

YPH:

YPC_1667

YPA:

YPA_1958

YPN:

YPN_2056

YPM:

YP_2279

YPP:	YPDSF_1875

YPG:	YpAngola_A1721

YPZ:

YPZ3_2110

YPT:	A1122_13840

YPD:

YPD4_2151

YPX:

YPD8_2236

YPW:	CH59_2(fae)

YPJ:	CH55_148(fae)

YPV:	BZ15_1068(fae)

YPL:	CH46_2648(fae)

YPS:

YPTB2503

YPO:	BZ17_4134(fae)

YPI:	YpsIP31758_1542

YPY:

YPK_1651

YPB:

YPTS_2597

YPQ:	DJ40_4039(fae)

YPU:	BZ21_1799(fae)

YPR:	BZ20_3706(fae)

YPC:	BZ23_2084(fae)

YPF:	BZ19_1865(fae)

SPE:

Spro_2468

SFO:	Z042_11985

EAME:

GXP68_21270(fae)

DYE:	EO087_01975(fae)

DCS:	ISN74_16145(fae)

PCQ:	PcP3B5_53090(fae)

PMUI:

G4G71_08165(fae)

PPAA:

B7D75_22335(fae)

PFN:	HZ99_00560

PMAN:

OU5_6013

PTV:	AA957_01335

PAZO:

AYR47_21265

PCZ:	PCL1606_37300

PSEM:

TO66_12085

PSOS:

POS17_3724

PANR:

A7J50_2664

PYM:	AK972_2518

PKE:	DLD99_16655(fae)

POJ:	PtoMrB4_31270

PZE:	HU754_017895(fae)

PMOE:

HV782_012460(fae)

PWY:	HU734_015060(fae)

PIZ:	LAB08_R38260(fae)

PPEG:

KUA23_13080(fae)

PFIT:

KJY40_17215(fae)

MCA:	MCA2778 MCA2866

METU:

GNH96_00505(fae) GNH96_03515(fae)

MMT:	Metme_0102 Metme_2268 Metme_2975

MDN:	JT25_013205 JT25_015530 JT25_019350 JT25_022600

MDH:	AYM39_06220 AYM39_08555 AYM39_18505

MKO:	MKLM6_1265 MKLM6_1781 MKLM6_3742

METL:

U737_02620(fae) U737_05120(fae) U737_11025(fae) U737_14805(fae)

MPAD:

KEF85_10810(fae) KEF85_11460(fae)

MELL:

IVG45_03945(fae) IVG45_04040(fae) IVG45_15590(fae)

MRP:	NM686_014175(fae) NM686_021075(fae)

MAH:	MEALZ_0850(fae2) MEALZ_1456(fae) MEALZ_2428(fae)

MBUR:

EQU24_09110(fae) EQU24_10305(fae) EQU24_13345(fae) EQU24_14320(fae)

MPSY:

CEK71_02655(fae) CEK71_20175(fae)

MMAI:

sS8_0613 sS8_3390 sS8_4382 sS8_4455 sS8_5134

MMOB:

F6R98_04980(fae) F6R98_15000(fae) F6R98_19005(fae)

MEIN:

methR_P2096 methR_P2098

MOZ:	MoryE10_27530 MoryE10_28840

MISZ:

MishRS11D_40160

MESL:

KKZ03_09100(fae) KKZ03_09125(fae) KKZ03_16955(fae)

MEJ:	Q7A_1764(fae) Q7A_1938(fae) Q7A_47(fae)

MEC:	Q7C_1775 Q7C_2566 Q7C_8

MPIN:

LGT42_002840(fae) LGT42_008835(fae) LGT42_009450(fae)

BLEP:

AL038_08760(fae) AL038_13645(fae)

NHL:

Nhal_3879

NWR:	E3U44_04660(fae)

TSY:	THSYN_31765 THSYN_32775

SEDS:

AAY24_01665

AMAH:

DLM_3633

RIN:	ACS15_3974(fae)

BUR:	Bcep18194_B2825

BCEN:

DM39_4730(fae)

BMK:	DM80_4395(fae)

BCEP:

APZ15_26560

BPYR:

ABD05_19745

BDF:	WI26_16315

BTEI:

WS51_00910

BSTL:

BBJ41_29385

BANN:

JFN94_19620(fae)

BARI:

NLX30_21065(fae)

BUG:	BC1001_3951 BC1001_4826

BGF:	BC1003_4670 BC1003_5452

BGD:	bgla_2g26440

BYI:	BYI23_B014430 BYI23_D012810 BYI23_D013000

BUE:	BRPE67_BCDS01420 BRPE67_DCDS01480 BRPE67_DCDS01680

BUQ:	AC233_22040 AC233_25905

BUM:	AXG89_19490

BUI:	AX768_29255

BXE:	Bxe_B2436(fae)

BXB:	DR64_4764(fae)

BPH:	Bphy_5474 Bphy_6487

BGE:	BC1002_3220

BPX:	BUPH_00535 BUPH_01391

BPY:	Bphyt_6933

BUZ:	AYM40_21715

BFN:	OI25_5387(fae)

BCAI:

K788_0000879 K788_0003216 K788_0008560

PSPW:

BJG93_19410(fae)

PARA:

BTO02_24110

PARB:

CJU94_31180(fae)

PHS:	C2L64_25890(fae) C2L64_32370(fae) C2L64_38280(fae)

PTER:

C2L65_22495(fae) C2L65_29025(fae) C2L65_34275(fae)

PGP:	CUJ91_21485(fae) CUJ91_30315(fae)

PCJ:	CUJ87_23435(fae) CUJ87_27745(fae)

PTS:	CUJ90_21415(fae) CUJ90_28055(fae)

PCAF:

DSC91_002948

PMEG:

FNZ07_05665(fae)

PGIS:

I6I06_18070(fae) I6I06_22480(fae)

PACP:

FAZ97_24650(fae)

PACS:

FAZ98_28970(fae)

PEW:	KZJ38_25855(fae)

PDIO:

PDMSB3_0626.1(fae)

PBRY:

NDK50_35955(fae)

CABA:

SBC2_54700(fae)

BUO:	BRPE64_BCDS01560 BRPE64_DCDS00870 BRPE64_DCDS01190

RHG:	EXZ61_07325(fae)

VAP:	Vapar_3063 Vapar_3079

VPD:	VAPA_1c31280 VAPA_1c31430(fae)

MPT:	Mpe_A2612 Mpe_A2627

METP:

C1M51_15005(fae) C1M51_15080(fae)

RGE:	RGE_40280(fae) RGE_40430

ATER:

MW290_07035(fae) MW290_07960(fae)

LCH:	Lcho_3132 Lcho_3147

CARE:

LT85_1433

AON:	DEH84_18915(fae)

METR:

BSY238_1372(fae) BSY238_3289(fae)

MFA:	Mfla_1652 Mfla_2364 Mfla_2524 Mfla_2543

MMB:	Mmol_0024 Mmol_1253 Mmol_2056

MEH:	M301_0896 M301_1343 M301_1423 M301_2758

MEI:	Msip34_1495 Msip34_1496 Msip34_2416 Msip34_2608

MEP:	MPQ_1558 MPQ_1559 MPQ_2354 MPQ_2546

MBAC:

BN1209_1682

MEU:	ACJ67_01845 ACJ67_07775 ACJ67_12360

MPAU:

ZMTM_09080(fae_1) ZMTM_11900 ZMTM_24180(fae_2)

SPLB:

SFPGR_25950

AZA:	AZKH_p0238(fae) AZKH_p0270(fae)

AZD:	CDA09_11330(fae) CDA09_11535(fae)

THU:	AC731_004855 AC731_004925

THK:	CCZ27_13170(fae) CCZ27_13240(fae)

DDZ:	DSYM_25720

MCI:	Mesci_4263

MCIC:

A4R28_05740

MESW:

A9K65_023635

MESM:

EJ066_29735(fae)

MOH:	IHQ72_13860(fae)

PHT:	BLM14_23805 BLM14_29225

PHYL:

HB779_00970(fae)

RGA:	RGR602_PC01571(fae)

XAU:	Xaut_0032 Xaut_1365 Xaut_1627

XDI:	EZH22_07400(fae) EZH22_16240(fae) EZH22_24935(fae)

AZC:

AZC_0890

SNO:	Snov_0740 Snov_1050

STAR:

G3545_09615(fae) G3545_09875(fae)

LNE:	FZC33_09350(fae)

ANC:	GBB76_00705(fae) GBB76_01750(fae)

APRA:

G3A50_17700(fae)

APOL:

K9D25_19005(fae) K9D25_20315(fae)

MEA:	Mex_1p1339(fae) Mex_1p1766(fae) Mex_1p3356(fae)

MDI:	METDI2111(fae) METDI2518(fae) METDI3927(fae)

MEX:	Mext_1450 Mext_1834 Mext_3143

MCH:	Mchl_1725 Mchl_2169 Mchl_3465

MPO:	Mpop_1445 Mpop_1785 Mpop_3340

MZA:	B2G69_14880 B2G69_16830 B2G69_23280

MRD:	Mrad2831_0533 Mrad2831_2016 Mrad2831_4467

MET:	M446_4369 M446_5783

MNO:	Mnod_3403 Mnod_4915 Mnod_6007

MOR:	MOC_0688 MOC_2407 MOC_5126(fae)

META:

Y590_06750 Y590_08785 Y590_15320

MAQU:

Maq22A_1p31155(fae) Maq22A_c16490(fae)

MPHY:

MCBMB27_00110 MCBMB27_01618 MCBMB27_03977

MEE:	DA075_05745(fae) DA075_25935

METD:

C0214_08220(fae) C0214_10540(fae) C0214_17445 C0214_27315

METX:

A3862_04400 A3862_09065 A3862_20975 A3862_28285

METS:

DK389_14645(fae) DK389_20670(fae)

METI:

DK427_18460(fae) DK427_19030 DK427_23350(fae)

MMES:

MMSR116_16705(fae) MMSR116_21455 MMSR116_24175(fae)

MTEA:

DK419_01760 DK419_10810(fae)

MIND:

mvi_38650 mvi_45940

MOG:	MMB17_11245(fae) MMB17_20010(fae) MMB17_24565

MTAD:

M6G65_10850(fae) M6G65_13650(fae) M6G65_20260

MSL:	Msil_1799 Msil_2390

MTUN:

MTUNDRAET4_1948(fae) MTUNDRAET4_2726(fae) MTUNDRAET4_3737 MTUNDRAET4_4163

MLG:	CWB41_00705 CWB41_04145(fae) CWB41_06060(fae) CWB41_07900(fae)

HDN:	Hden_1474 Hden_1875 Hden_2126

HDT:	HYPDE_31273 HYPDE_33638

HMC:	HYPMC_1396(fae) HYPMC_1624(fae) HYPMC_2181(fae) HYPMC_3439

HNI:	W911_00020 W911_13190

FIL:	BN1229_v1_0583(fae) BN1229_v1_1275

FIY:	BN1229_v1_0585(fae) BN1229_v1_1273

MCG:	GL4_1783 GL4_3256

METG:

HT051_03555(fae) HT051_10195(fae)

MSC:	BN69_0705 BN69_1051(fae) BN69_1356(fae) BN69_1357

MBRY:

B1812_14735 B1812_14740 B1812_18000

MROS:

EHO51_09550(fae) EHO51_11440(fae) EHO51_13070(fae) EHO51_13075(fae)

MHEY:

H2LOC_003830(fae) H2LOC_003835(fae) H2LOC_014910(fae) H2LOC_019690

MPAR:

F7D14_05065(fae) F7D14_05070(fae) F7D14_07930(fae) F7D14_11835(fae) F7D14_12840(fae)

MTW:	CQW49_09280(fae) CQW49_13475(fae) CQW49_13480(fae)

CMET:

K6K41_20695

PLEO:

OHA_1_02036(fae)

HDI:	HDIA_0174(fae_1) HDIA_2315(fae_2) HDIA_3044(fae_3)

AALA:

IGS74_05765(fae)

AALM:

LUX29_06520(fae)

AUZ:	Sa4125_16230 Sa4125_32480(fae) Sa4125_38560

AUB:	LXB15_00625(fae) LXB15_00965(fae) LXB15_17780

JIE:	OH818_07255(fae) OH818_26010(fae) OH818_26295

GBE:	GbCGDNIH1_0524 GbCGDNIH1_0770

GBH:	GbCGDNIH2_0524 GbCGDNIH2_0770

GBC:	GbCGDNIH3_0524 GbCGDNIH3_0770

GBS:	GbCGDNIH4_0524 GbCGDNIH4_0770

ALI:	AZOLI_p10696(fae1) AZOLI_p10865 AZOLI_p10886(fae2)

ABS:	AZOBR_p270080(fae)

ABQ:	ABAZ39_25485

ABF:	AMK58_22315

ATI:	AL072_27025

AZT:	TSH58p_01220(fae)

AZM:	DM194_17130(fae)

AOZ:	HUE56_08815(fae) HUE56_08905(fae)

AARE:

D3093_24945(fae)

SVL:	Strvi_4314

SRC:	M271_03745

SMAL:

SMALA_7629

SSOI:

I1A49_41920(fae)

SCLF:

BB341_29110

SNQ:	CP978_00965(fae)

SSPO:

DDQ41_05910(fae)

SCHA:

CP983_06220(fae)

SBRO:

GQF42_39910(fae)

SLF:	JEQ17_43600(fae)

LXY:	O159_21150

MIX:	AB663_001784

MOY:	CVS54_02583(fae)

MLIQ:

NMQ05_12395(fae)

RLA:	Rhola_00000810

RPLA:

A4Z71_00360

ASOI:

MTP13_05490(fae)

CPHY:

B5808_02390

LTR:	EVS81_09120(fae)

LEB:	G7066_15115(fae)

LVI:	G7068_09010(fae)

LINS:

G7067_06720(fae)

LLUT:

K1X41_04055(fae)

MANT:

BHD05_05170

GSD:	M3M28_08760(fae)

ARR:	ARUE_c06130

ARN:	CGK93_03385(fae)

AAU:

AAur_0648

NMES:

H9L09_15330(fae)

AMD:

AMED_6210

AMN:

RAM_31860

AMM:

AMES_6120

AMZ:

B737_6120

ATHM:

L1857_14675(fae)

LED:	BBK82_32490

ABAI:

IMCC26256_111856

RUB:	GBA63_16325(fae)

RBA:	RB10297(fae) RB9338(fae)

PSL:	Psta_2526 Psta_2658

PIR:	VN12_03550(fae_1) VN12_18235(fae_2)

RUL:	UC8_03170(fae_1) UC8_11080(fae_2)

RML:	FF011L_25930(fae_1) FF011L_55020(fae_2)

MFF:	MFFC18_24490(fae_1) MFFC18_35380(fae_2)

ROL:	CA51_24730(fae)

AHEL:

Q31a_40200(fae)

LCRE:

Pla8534_42870(fae)

AAGG:

ETAA8_15620(fae_1) ETAA8_20230(fae_2)

BVO:	Pan97_44420(fae)

RLC:	K227x_30190(fae_1) K227x_52050(fae_2)

LPAV:

PLANPX_0556

AMUC:

Pan181_45480(fae)

PND:	Pla175_09770 Pla175_50940(fae)

AMOB:

HG15A2_05200(fae_1) HG15A2_07300(fae_2)

PLM:	Plim_1285 Plim_3519

PEH:	Spb1_04510(fae_1) Spb1_19420(fae_2)

PBS:	Plabr_0087

PLS:	VT03_23680(fae_1) VT03_31915(fae_2)

PLH:	VT85_01025(fae_1) VT85_19960(fae_2)

FMR:	Fuma_01647(fae_1) Fuma_04816(fae_2)

GMR:	GmarT_10980(fae_1) GmarT_49910(fae_2)

GIM:	F1728_09275(fae) F1728_20510(fae)

GPN:	Pan110_10780(fae_1) Pan110_49650(fae_2)

MRI:	Mal4_08490(fae)

SDYN:

Mal52_22840(fae_1) Mal52_35040(fae_2)

PLON:

Pla110_13110(fae)

GES:	VT84_01760(fae_1) VT84_22510(fae_2)

GOG:	C1280_14275(fae) C1280_25120(fae)

GMS:	SOIL9_33500 SOIL9_33510 SOIL9_75910

TIM:	GMBLW1_20440 GMBLW1_46820

LRS:	PX52LOC_00083 PX52LOC_07250(fae)

FTJ:	FTUN_3066 FTUN_8061

ULI:	ETAA1_04000(fae_1) ETAA1_52360(fae_2)

TSPH:

KIH39_06790 KIH39_14265(fae)

IPA:

Isop_1013

SACI:

Sinac_4606 Sinac_4954

PBOR:

BSF38_03483(fae_1) BSF38_04915(fae_2)

AGV:	OJF2_60590(fae_1) OJF2_74120(fae_2)

MOX:	DAMO_0454(fae)

MJA:

MJ_1447

MFE:	Mefer_1012

MVU:	Metvu_1176

MFS:	MFS40622_0906

MIF:	Metin_1286

MJH:	JH146_0570

MESG:

MLAUSG7_1449(fae-hps)

MESA:

MLASG1_0987(fae-hps)

MIG:	Metig_0664

MAE:

Maeo_0614

MOK:	Metok_1249

METF:

CFE53_04300

MTH:

MTH_1474

MMG:	MTBMA_c00570(fae_hps)

MWO:	MWSIV6_0011(fae-hps)

MTEE:

MTTB_00460

METC:

MTCT_1339

METE:

tca_01421(fae)

METZ:

METMT2_1368

METK:

FVF72_04250

MTHM:

FZP57_00015

METJ:

FZP68_01675

MST:

Msp_1498

METB:

AW729_07875

MRU:

mru_2131

MSI:

Msm_1213

MEB:

Abm4_0046

MMIL:

sm9_0074

MEYE:

TL18_10355

MOL:

YLM1_1686

MARO:

MarbSA_11880

METV:

K4897_05320

MEL:	Metbo_0071

MEW:	MSWAN_0017 MSWAN_2043

METH:

MBMB1_0009(fae-hps) MBMB1_1667

MFC:	BRM9_0060 BRM9_0918(fae)

MFI:	DSM1535_0886 DSM1535_1378(fae-hps)

MCUB:

MCBB_0021(fae-hps_1) MCBB_1971(fae-hps_3)

MSUB:

BK009_09760

METN:

BK008_05430

METT:

CIT01_05685(fae)

METO:

CIT02_07030(fae) CIT02_09565(fae)

MEME:

HYG87_06650

MFV:

Mfer_0572

MKA:

MK1275

AFU:

AF_1305

AFG:	AFULGI_00014160

APO:	Arcpr_1052

AVE:	Arcve_1411

AST:	Asulf_01087

FPL:	Ferp_1735 Ferp_2129

GAC:

GACE_2158

GAH:	GAH_00575 GAH_00673

MBA:	Mbar_A0935 Mbar_A2060

MBY:	MSBRM_0067 MSBRM_2444

MBW:	MSBRW_0061 MSBRW_2677

MBAR:

MSBR2_2313 MSBR2_2410

MBAK:

MSBR3_2375 MSBR3_2463

MAC:	MA_3006 MA_4608

MMA:

MM_1279

MMAZ:

MmTuc01_1325

MMJ:	MSMAS_0037

MMAC:

MSMAC_0037

MVC:	MSVAZ_0074 MSVAZ_2649

MEK:	MSKOL_0070 MSKOL_2653

MLS:	MSLAZ_0035 MSLAZ_1178

METM:

MSMTP_0040

MEF:	MSWH1_0036

MEQ:	MSWHS_0037

MSJ:	MSSAC_0046 MSSAC_3267

MSZ:	MSSIH_0039 MSSIH_1163

MSW:	MSSIT_0039 MSSIT_1211

MTHR:

MSTHT_0003 MSTHT_0011 MSTHT_1787

MTHE:

MSTHC_0717 MSTHC_1499

MHOR:

MSHOH_0037

MFZ:	AOB57_002495(fae) AOB57_008160

MBU:	Mbur_0367 Mbur_1994(fae-hps)

MMET:

MCMEM_0222 MCMEM_1329

MELO:

J7W08_05295(fae) J7W08_11180

MMH:

Mmah_0234

MHAZ:

BHR79_02340

MEV:	Metev_1698

MZH:	Mzhil_0262

MPY:

Mpsy_1425

MZI:	HWN40_01665 HWN40_05490(fae)

MHZ:	Metho_0261 Metho_1162

MTP:

Mthe_0988

MCJ:	MCON_1033(faeA) MCON_1383

MHI:	Mhar_0019 Mhar_0339

MHU:

Mhun_1628

MRTJ:

KHC33_06360

MLA:

Mlab_0996

MEM:	Memar_0975 Memar_1085

MBG:	BN140_1075 BN140_1906

MEMA:

MMAB1_1386(fae-hps) MMAB1_2454(faeA)

MCHK:

MchiMG62_10980 MchiMG62_12270(fae)

MSUM:

OH143_11600(fae) OH143_12150

MPI:

Mpet_0801

MEND:

L6E24_11130

MAQE:

RJ40_03655

MOU:	OU421_12595

MBN:

Mboo_0772

MFO:	Metfor_2057

MPL:

Mpal_1036

MPD:	MCP_2904(fae_hps)

MEZ:	Mtc_0381(fae)

RCI:	RCIX1876(fae) RCIX775(fae-hps)

HARA:

AArcS_1663

MELU:

MTLP_05990(fae_1) MTLP_06280(fae_2)

MEAM:

MU439_01845

IAG:

Igag_0274

CCAI:

NAS2_0542

BARC:

AOA65_1083(faeB-hpsB)

BARB:

AOA66_1278

FLT:	Sv326_0214 Sv326_0884

LOKI:

Lokiarch_06870 Lokiarch_17920 Lokiarch_47420

PSYT:

DSAG12_03237(faeB-hpsB)

» show all

Reference

1 [PMID:11073907]

Authors

Vorholt JA, Marx CJ, Lidstrom ME, Thauer RK

Title

Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol.

Journal

J Bacteriol 182:6645-50 (2000)
DOI:10.1128/JB.182.23.6645-6650.2000

Sequence

[mea:Mex_1p1766]

Reference

2 [PMID:15632161]

Authors

Acharya P, Goenrich M, Hagemeier CH, Demmer U, Vorholt JA, Thauer RK, Ermler U

Title

How an enzyme binds the C1 carrier tetrahydromethanopterin. Structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1.

Journal

J Biol Chem 280:13712-9 (2005)
DOI:10.1074/jbc.M412320200

Sequence

[mea:Mex_1p1766]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.147

IUBMB Enzyme Nomenclature:

4.2.1.147

ExPASy - ENZYME nomenclature database:

4.2.1.147

BRENDA, the Enzyme Database:

4.2.1.147

DBGET integrated database retrieval system