KEGG   ENZYME: 4.2.1.147
Entry
EC 4.2.1.147                Enzyme                                 
Name
5,6,7,8-tetrahydromethanopterin hydro-lyase;
formaldehyde-activating enzyme
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
5,6,7,8-tetrahydromethanopterin hydro-lyase (formaldehyde-adding, tetrahydromethanopterin-forming)
Reaction(IUBMB)
5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O [RN:R08058]
Reaction(KEGG)
R08058
Substrate
5,6,7,8-tetrahydromethanopterin [CPD:C01217];
formaldehyde [CPD:C00067]
Product
5,10-methylenetetrahydromethanopterin [CPD:C04377];
H2O [CPD:C00001]
Comment
Found in methylotrophic bacteria and methanogenic archaea.
History
EC 4.2.1.147 created 2014
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10713  5,6,7,8-tetrahydromethanopterin hydro-lyase
K13812  bifunctional enzyme Fae/Hps
Genes
KMIVW41_19435
CNTJT31_16265
CEMLH23_01445
CENLH86_01435
YPEYPO2460
YPKy1729
YPHYPC_1667
YPAYPA_1958
YPNYPN_2056
YPMYP_2279
YPPYPDSF_1875
YPGYpAngola_A1721
YPZYPZ3_2110
YPTA1122_13840
YPDYPD4_2151
YPXYPD8_2236
YPWCH59_2(fae)
YPJCH55_148(fae)
YPVBZ15_1068(fae)
YPLCH46_2648(fae)
YPSYPTB2503
YPOBZ17_4134(fae)
YPIYpsIP31758_1542
YPYYPK_1651
YPBYPTS_2597
YPQDJ40_4039(fae)
YPUBZ21_1799(fae)
YPRBZ20_3706(fae)
YPCBZ23_2084(fae)
YPFBZ19_1865(fae)
SPESpro_2468
SFOZ042_11985
EAMEGXP68_21270(fae)
DYEEO087_01975(fae)
DCSISN74_16145(fae)
PCQPcP3B5_53090(fae)
PMUIG4G71_08165(fae)
PFNHZ99_00560
PMANOU5_6013
PTVAA957_01335
PAZOAYR47_21265
PCZPCL1606_37300
PSEMTO66_12085
PSOSPOS17_3724
PANRA7J50_2664
PYMAK972_2518
PKEDLD99_16655(fae)
POJPtoMrB4_31270
PZEHU754_017895(fae)
PMOEHV782_012460(fae)
PWYHU734_015060(fae)
MCAMCA2778 MCA2866
METUGNH96_00505(fae) GNH96_03515(fae)
MMTMetme_0102 Metme_2268 Metme_2975
MDNJT25_013205 JT25_015530 JT25_019350 JT25_022600
MDHAYM39_06220 AYM39_08555 AYM39_18505
MKOMKLM6_1265 MKLM6_1781 MKLM6_3742
METLU737_02620(fae) U737_05120(fae) U737_11025(fae) U737_14805(fae)
MPADKEF85_10810(fae) KEF85_11460(fae)
MELLIVG45_03945(fae) IVG45_04040(fae) IVG45_15590(fae)
MAHMEALZ_0850(fae2) MEALZ_1456(fae) MEALZ_2428(fae)
MBUREQU24_09110(fae) EQU24_10305(fae) EQU24_13345(fae) EQU24_14320(fae)
MPSYCEK71_02655(fae) CEK71_20175(fae)
MMAIsS8_0613 sS8_3390 sS8_4382 sS8_4455 sS8_5134
MMOBF6R98_04980(fae) F6R98_15000(fae) F6R98_19005(fae)
MEINmethR_P2096 methR_P2098
MOZMoryE10_27530 MoryE10_28840
MISZMishRS11D_40160
MESLKKZ03_09100(fae) KKZ03_16955(fae)
MEJQ7A_1764(fae) Q7A_1938(fae) Q7A_47(fae)
MECQ7C_1775 Q7C_2566 Q7C_8
BLEPAL038_08760(fae) AL038_13645(fae)
NHLNhal_3879
NWRE3U44_04660(fae)
TSYTHSYN_31765 THSYN_32775
SEDSAAY24_01665
AMAHDLM_3633
RINACS15_3974(fae)
BURBcep18194_B2825
BCENDM39_4730(fae)
BMKDM80_4395(fae)
BCEPAPZ15_26560
BPYRABD05_19745
BDFWI26_16315
BTEIWS51_00910
BSTLBBJ41_29385
BANNJFN94_19620(fae)
BUGBC1001_3951 BC1001_4826
BGFBC1003_4670 BC1003_5452
BGDbgla_2g26440
BYIBYI23_B014430 BYI23_D012810 BYI23_D013000
BUEBRPE67_BCDS01420 BRPE67_DCDS01480 BRPE67_DCDS01680
BUQAC233_22040 AC233_25905
BUMAXG89_19490
BUIAX768_29255
BXEBxe_B2436(fae)
BXBDR64_4764(fae)
BPHBphy_5474 Bphy_6487
BGEBC1002_3220
BPXBUPH_00535 BUPH_01391
BPYBphyt_6933
BUZAYM40_21715
BFNOI25_5387(fae)
BCAIK788_0000879 K788_0003216 K788_0008560
PSPWBJG93_19410(fae)
PARABTO02_24110
PARBCJU94_31180(fae)
PHSC2L64_25890(fae) C2L64_32370(fae) C2L64_38280(fae)
PTERC2L65_22495(fae) C2L65_29025(fae) C2L65_34275(fae)
PGPCUJ91_21485(fae) CUJ91_30315(fae)
PCJCUJ87_23435(fae) CUJ87_27745(fae)
PTSCUJ90_21415(fae) CUJ90_28055(fae)
PCAFDSC91_002948
PMEGFNZ07_05665(fae)
PGISI6I06_18070(fae) I6I06_22480(fae)
PACPFAZ97_24650(fae)
PACSFAZ98_28970(fae)
PEWKZJ38_25855(fae)
PDIOPDMSB3_0626.1(fae)
CABASBC2_54700(fae)
BUOBRPE64_BCDS01560 BRPE64_DCDS00870 BRPE64_DCDS01190
RHGEXZ61_07325(fae)
VAPVapar_3063 Vapar_3079
VPDVAPA_1c31280 VAPA_1c31430(fae)
MPTMpe_A2612 Mpe_A2627
METPC1M51_15005(fae) C1M51_15080(fae)
CARELT85_1433
LCHLcho_3132 Lcho_3147
RGERGE_40280(fae) RGE_40430
AONDEH84_18915(fae)
METRBSY238_1372(fae) BSY238_3289(fae)
MFAMfla_1652 Mfla_2364 Mfla_2524 Mfla_2543
MMBMmol_0024 Mmol_1253 Mmol_2056
MEHM301_0896 M301_1343 M301_1423 M301_2758
MEIMsip34_1495 Msip34_1496 Msip34_2416 Msip34_2608
MEPMPQ_1558 MPQ_1559 MPQ_2354 MPQ_2546
MBACBN1209_1682
MEUACJ67_01845 ACJ67_07775 ACJ67_12360
MPAUZMTM_09080(fae_1) ZMTM_11900 ZMTM_24180(fae_2)
SPLBSFPGR_25950
AZAAZKH_p0238(fae) AZKH_p0270(fae)
AZDCDA09_11330(fae) CDA09_11535(fae)
THUAC731_004855 AC731_004925
THKCCZ27_13170(fae) CCZ27_13240(fae)
DDZDSYM_25720
MCIMesci_4263
MESWA9K65_023635
MESMEJ066_29735(fae)
PHTBLM14_23805 BLM14_29225
RGARGR602_PC01571(fae)
XAUXaut_0032 Xaut_1365 Xaut_1627
XDIEZH22_07400(fae) EZH22_16240(fae) EZH22_24935(fae)
AZCAZC_0890
SNOSnov_0740 Snov_1050
STARG3545_09615(fae) G3545_09875(fae)
LNEFZC33_09350(fae)
ANCGBB76_00705(fae) GBB76_01750(fae)
APRAG3A50_17700(fae)
MEAMex_1p1339(fae) Mex_1p1766(fae) Mex_1p3356(fae)
MDIMETDI2111(fae) METDI2518(fae) METDI3927(fae)
MEXMext_1450 Mext_1834 Mext_3143
MCHMchl_1725 Mchl_2169 Mchl_3465
MPOMpop_1445 Mpop_1785 Mpop_3340
MZAB2G69_14880 B2G69_16830 B2G69_23280
MRDMrad2831_0533 Mrad2831_2016 Mrad2831_4467
METM446_4369 M446_5783
MNOMnod_3403 Mnod_4915 Mnod_6007
MORMOC_0688 MOC_2407 MOC_5126(fae)
METAY590_06750 Y590_08785 Y590_15320
MAQUMaq22A_1p31155(fae) Maq22A_c16490(fae)
MPHYMCBMB27_00110 MCBMB27_01618 MCBMB27_03977
MEEDA075_05745(fae) DA075_25935
METDC0214_08220(fae) C0214_10540(fae) C0214_17445 C0214_27315
METXA3862_04400 A3862_09065 A3862_20975 A3862_28285
METSDK389_14645(fae) DK389_20670(fae)
METIDK427_18460(fae) DK427_19030 DK427_23350(fae)
MMESMMSR116_16705(fae) MMSR116_21455 MMSR116_24175(fae)
MTEADK419_01760 DK419_10810(fae)
MINDmvi_38650 mvi_45940
MOGMMB17_11245(fae) MMB17_20010(fae) MMB17_24565
MSLMsil_1799 Msil_2390
MTUNMTUNDRAET4_1948(fae) MTUNDRAET4_2726(fae) MTUNDRAET4_3737 MTUNDRAET4_4163
MLGCWB41_00705 CWB41_04145(fae) CWB41_06060(fae) CWB41_07900(fae)
HDNHden_1474 Hden_1875 Hden_2126
HDTHYPDE_31273 HYPDE_33638
HMCHYPMC_1396(fae) HYPMC_1624(fae) HYPMC_2181(fae) HYPMC_3439
HNIW911_00020 W911_13190
FILBN1229_v1_0583(fae) BN1229_v1_1275
FIYBN1229_v1_0585(fae) BN1229_v1_1273
MCGGL4_1783 GL4_3256
METGHT051_03555(fae) HT051_10195(fae)
MSCBN69_0705 BN69_1051(fae) BN69_1356(fae) BN69_1357
MBRYB1812_14735 B1812_14740 B1812_18000
MROSEHO51_09550(fae) EHO51_11440(fae) EHO51_13070(fae) EHO51_13075(fae)
MHEYH2LOC_003830(fae) H2LOC_003835(fae) H2LOC_014910(fae) H2LOC_019690
MPARF7D14_05065(fae) F7D14_05070(fae) F7D14_07930(fae) F7D14_11835(fae) F7D14_12840(fae)
MTWCQW49_09280(fae) CQW49_13475(fae) CQW49_13480(fae)
CMETK6K41_20695
PLEOOHA_1_02036(fae)
HDIHDIA_0174(fae_1) HDIA_2315(fae_2) HDIA_3044(fae_3)
AALAIGS74_05765(fae)
AALMLUX29_06520(fae)
AUBLXB15_00625(fae) LXB15_00965(fae) LXB15_17780
GBEGbCGDNIH1_0524 GbCGDNIH1_0770
GBHGbCGDNIH2_0524 GbCGDNIH2_0770
GBCGbCGDNIH3_0524 GbCGDNIH3_0770
GBSGbCGDNIH4_0524 GbCGDNIH4_0770
ALIAZOLI_p10696(fae1) AZOLI_p10865 AZOLI_p10886(fae2)
ABSAZOBR_p270080(fae)
ABQABAZ39_25485
ABFAMK58_22315
ATIAL072_27025
AZTTSH58p_01220(fae)
AZMDM194_17130(fae)
AOZHUE56_08815(fae) HUE56_08905(fae)
SVLStrvi_4314
SRCM271_03745
SMALSMALA_7629
SSOII1A49_41920(fae)
SCLFBB341_29110
SNQCP978_00965(fae)
SSPODDQ41_05910(fae)
SCHACP983_06220(fae)
SBROGQF42_39910(fae)
LXYO159_21150
MIXAB663_001784
MOYCVS54_02583(fae)
RLARhola_00000810
RPLAA4Z71_00360
CPHYB5808_02390
LTREVS81_09120(fae)
LEBG7066_15115(fae)
LVIG7068_09010(fae)
LINSG7067_06720(fae)
LLUTK1X41_04055(fae)
MANTBHD05_05170
ARRARUE_c06130
ARNCGK93_03385(fae)
AAUAAur_0648
NMESH9L09_15330(fae)
AMDAMED_6210
AMNRAM_31860
AMMAMES_6120
AMZB737_6120
LEDBBK82_32490
ABAIIMCC26256_111856
RUBGBA63_16325(fae)
RBARB10297(fae) RB9338(fae)
PSLPsta_2526 Psta_2658
PIRVN12_03550(fae_1) VN12_18235(fae_2)
RULUC8_03170(fae_1) UC8_11080(fae_2)
RMLFF011L_25930(fae_1) FF011L_55020(fae_2)
MFFMFFC18_24490(fae_1) MFFC18_35380(fae_2)
ROLCA51_24730(fae)
AHELQ31a_40200(fae)
LCREPla8534_42870(fae)
AAGGETAA8_15620(fae_1) ETAA8_20230(fae_2)
BVOPan97_44420(fae)
RLCK227x_30190(fae_1) K227x_52050(fae_2)
LPAVPLANPX_0556
AMUCPan181_45480(fae)
PNDPla175_09770 Pla175_50940(fae)
AMOBHG15A2_05200(fae_1) HG15A2_07300(fae_2)
PLMPlim_1285 Plim_3519
PEHSpb1_04510(fae_1) Spb1_19420(fae_2)
PBSPlabr_0087
PLSVT03_23680(fae_1) VT03_31915(fae_2)
PLHVT85_01025(fae_1) VT85_19960(fae_2)
FMRFuma_01647(fae_1) Fuma_04816(fae_2)
GMRGmarT_10980(fae_1) GmarT_49910(fae_2)
GIMF1728_09275(fae) F1728_20510(fae)
GPNPan110_10780(fae_1) Pan110_49650(fae_2)
MRIMal4_08490(fae)
SDYNMal52_22840(fae_1) Mal52_35040(fae_2)
PLONPla110_13110(fae)
GESVT84_01760(fae_1) VT84_22510(fae_2)
GOGC1280_14275(fae) C1280_25120(fae)
GMSSOIL9_33500 SOIL9_33510 SOIL9_75910
TIMGMBLW1_20440 GMBLW1_46820
LRSPX52LOC_00083 PX52LOC_07250(fae)
FTJFTUN_3066 FTUN_8061
ULIETAA1_04000(fae_1) ETAA1_52360(fae_2)
TSPHKIH39_06790 KIH39_14265(fae)
IPAIsop_1013
SACISinac_4606 Sinac_4954
PBORBSF38_03483(fae_1) BSF38_04915(fae_2)
AGVOJF2_60590(fae_1) OJF2_74120(fae_2)
MOXDAMO_0454(fae)
MJAMJ_1447
MFEMefer_1012
MVUMetvu_1176
MFSMFS40622_0906
MIFMetin_1286
MJHJH146_0570
MESGMLAUSG7_1449(fae-hps)
MESAMLASG1_0987(fae-hps)
MIGMetig_0664
MAEMaeo_0614
MOKMetok_1249
METFCFE53_04300
MTHMTH_1474
MMGMTBMA_c00570(fae_hps)
METCMTCT_1339
MWOMWSIV6_0011(fae-hps)
METEtca_01421(fae)
METZMETMT2_1368
METKFVF72_04250
MTHMFZP57_00015
METJFZP68_01675
MSTMsp_1498
METBAW729_07875
MRUmru_2131
MSIMsm_1213
MEBAbm4_0046
MMILsm9_0074
MEYETL18_10355
MOLYLM1_1686
MAROMarbSA_11880
MELMetbo_0071
MEWMSWAN_0017 MSWAN_2043
METHMBMB1_0009(fae-hps) MBMB1_1667
MFCBRM9_0060 BRM9_0918(fae)
MFIDSM1535_0886 DSM1535_1378(fae-hps)
MCUBMCBB_0021(fae-hps_1) MCBB_1971(fae-hps_3)
MSUBBK009_09760
METNBK008_05430
METTCIT01_05685(fae)
METOCIT02_07030(fae) CIT02_09565(fae)
MEMEHYG87_06650
MFVMfer_0572
MKAMK1275
AFUAF_1305
AFGAFULGI_00014160
APOArcpr_1052
AVEArcve_1411
ASTAsulf_01087
FPLFerp_1735 Ferp_2129
GACGACE_2158
GAHGAH_00575 GAH_00673
MBAMbar_A0935 Mbar_A2060
MBYMSBRM_0067 MSBRM_2444
MBWMSBRW_0061 MSBRW_2677
MBARMSBR2_2313 MSBR2_2410
MBAKMSBR3_2375 MSBR3_2463
MACMA_3006 MA_4608
MMAMM_1279
MMAZMmTuc01_1325
MMJMSMAS_0037
MMACMSMAC_0037
MVCMSVAZ_0074 MSVAZ_2649
MEKMSKOL_0070 MSKOL_2653
MLSMSLAZ_0035 MSLAZ_1178
METMMSMTP_0040
MEFMSWH1_0036
MEQMSWHS_0037
MSJMSSAC_0046 MSSAC_3267
MSZMSSIH_0039 MSSIH_1163
MSWMSSIT_0039 MSSIT_1211
MTHRMSTHT_0003 MSTHT_0011 MSTHT_1787
MTHEMSTHC_0717 MSTHC_1499
MHORMSHOH_0037
MFZAOB57_002495(fae) AOB57_008160
MBUMbur_0367 Mbur_1994(fae-hps)
MMETMCMEM_0222 MCMEM_1329
MELOJ7W08_05295(fae) J7W08_11180
MMHMmah_0234
MHAZBHR79_02340
MEVMetev_1698
MZHMzhil_0262
MPYMpsy_1425
MZIHWN40_01665 HWN40_05490(fae)
MHZMetho_0261 Metho_1162
MTPMthe_0988
MCJMCON_1033(faeA) MCON_1383
MHIMhar_0019 Mhar_0339
MHUMhun_1628
MRTJKHC33_06360
MLAMlab_0996
MEMMemar_0975 Memar_1085
MBGBN140_1075 BN140_1906
MEMAMMAB1_1386(fae-hps) MMAB1_2454(faeA)
MCHKMchiMG62_10980 MchiMG62_12270(fae)
MPIMpet_0801
MBNMboo_0772
MFOMetfor_2057
MPLMpal_1036
MPDMCP_2904(fae_hps)
MEZMtc_0381(fae)
RCIRCIX1876(fae) RCIX775(fae-hps)
HARAAArcS_1663
MELUMTLP_05990(fae_1) MTLP_06280(fae_2)
MEAMMU439_01845
IAGIgag_0274
CCAINAS2_0542
BARCAOA65_1083(faeB-hpsB)
BARBAOA66_1278
FLTSv326_0214 Sv326_0884
LOKILokiarch_06870 Lokiarch_17920 Lokiarch_47420
PSYTDSAG12_03237(faeB-hpsB)
 » show all
Reference
1  [PMID:11073907]
  Authors
Vorholt JA, Marx CJ, Lidstrom ME, Thauer RK
  Title
Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol.
  Journal
J Bacteriol 182:6645-50 (2000)
DOI:10.1128/JB.182.23.6645-6650.2000
  Sequence
Reference
2  [PMID:15632161]
  Authors
Acharya P, Goenrich M, Hagemeier CH, Demmer U, Vorholt JA, Thauer RK, Ermler U
  Title
How an enzyme binds the C1 carrier tetrahydromethanopterin. Structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1.
  Journal
J Biol Chem 280:13712-9 (2005)
DOI:10.1074/jbc.M412320200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.147
IUBMB Enzyme Nomenclature: 4.2.1.147
ExPASy - ENZYME nomenclature database: 4.2.1.147
BRENDA, the Enzyme Database: 4.2.1.147

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