KEGG ENZYME: 4.2.1.149

ENZYME: 4.2.1.149

Entry

EC 4.2.1.149                Enzyme

Name

crotonobetainyl-CoA hydratase;
CaiD;
L-carnityl-CoA dehydratase

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

L-carnitinyl-CoA hydro-lyase [(E)-4-(trimethylammonio)but-2-enoyl-CoA-forming]

Reaction(IUBMB)

L-carnitinyl-CoA = (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O [RN:R10675]

Reaction(KEGG)

R10675

Substrate

L-carnitinyl-CoA [CPD:C20750]

Product

(E)-4-(trimethylammonio)but-2-enoyl-CoA [CPD:C20748];
H2O [CPD:C00001]

Comment

The enzyme is also able to use crotonyl-CoA as substrate, with low efficiency [2].

History

EC 4.2.1.149 created 2014

Orthology

K08299

crotonobetainyl-CoA hydratase

Genes

ECO:	b0036(caiD)

ECJ:	JW0035(caiD)

ECD:	ECDH10B_0037(caiD)

EBW:	BWG_0034(caiD)

ECOK:

ECMDS42_0029(caiD)

ECE:	Z0042(caiD)

ECS:	ECs_0039(caiD)

ECF:	ECH74115_0040(caiD)

ETW:	ECSP_0039(caiD)

ELX:	CDCO157_0038

EOI:	ECO111_0037(caiD)

EOJ:	ECO26_0037(caiD)

EOH:	ECO103_0038(caiD)

ECOO:

ECRM13514_0037(caiD)

ECOH:

ECRM13516_0039(caiD)

ESL:

O3K_21365

ESO:

O3O_04020

ESM:

O3M_21265

ECK:	EC55989_0036(caiD)

ECG:	E2348C_0037(caiD)

EOK:	G2583_0038(caiD)

ELR:	ECO55CA74_00180

ELH:

ETEC_0036

ECW:	EcE24377A_0038(caiD)

EUN:

UMNK88_36

ECP:

ECP_0036

ENA:	ECNA114_0023(caiD)

ECOS:

EC958_0170(caiD)

ECV:	APECO1_1945(caiD)

ECOA:

APECO78_03640

ECX:	EcHS_A0040(caiD)

ECM:	EcSMS35_0037(caiD)

ECY:

ECSE_0037

ECR:	ECIAI1_0038(caiD)

ECQ:	ECED1_0035(caiD)

EUM:	ECUMN_0038(caiD)

ECT:	ECIAI39_0037(caiD)

EOC:	CE10_0037(caiD)

EBR:	ECB_00040(caiD)

EBL:	ECD_00040(caiD)

EBE:	B21_00039(caiD)

EBD:

ECBD_3579

ECI:	UTI89_C0042(caiD)

EIH:	ECOK1_0035(caiD)

ECZ:	ECS88_0039(caiD)

ECC:	c0045(caiD)

ELO:	EC042_0038(caiD)

ELN:	NRG857_00190

ESE:

ECSF_0041

ECL:	EcolC_3619

EKO:	EKO11_3876

EKF:	KO11_00180(caiD)

EAB:	ECABU_c00410(caiD)

EDH:	EcDH1_3563

EDJ:	ECDH1ME8569_0035(caiD)

ELU:	UM146_22960

ELW:	ECW_m0036(caiD)

ELL:	WFL_00180(caiD)

ELC:	i14_0038(caiD)

ELD:	i02_0038(caiD)

ELP:	P12B_c0031(caiD)

ELF:	LF82_0260(caiD)

ECOL:

LY180_00185

ECOI:

ECOPMV1_00037(caiD_1)

ECOJ:

P423_00180

EFE:	EFER_0044(caiD)

EAL:	EAKF1_ch1386(caiD)

EMA:	C1192_12795

ESZ:	FEM44_14675(caiD)

STY:	STY0080(caiD)

STT:	t0071(caiD)

SEX:	STBHUCCB_810

SENT:

TY21A_00365

STM:	STM0070(caiD)

SEO:	STM14_0083(caiD)

SEV:	STMMW_00721

SEY:	SL1344_0071(caiD)

SEM:	STMDT12_C00710

SEJ:	STMUK_0071(caiD)

SEB:	STM474_0073(caiD)

SEF:	UMN798_0078(caiD)

SETU:

STU288_00350

SETC:

CFSAN001921_17070

SENR:

STMDT2_00711(caiD)

SEND:

DT104_00711(caiD)

SENI:

CY43_00345

SEEN:

SE451236_06360

SPT:	SPA0071(caiD)

SEK:

SSPA0067

SPQ:	SPAB_00086

SEI:	SPC_0075(caiD)

SEC:	SCH_0064(caiD)

SEH:	SeHA_C0074

SHB:

SU5_0705

SENH:

CFSAN002069_08880

SEEH:

SEEH1578_09380

SEE:	SNSL254_A0074

SENN:

SN31241_10490

SEW:	SeSA_A0078

SEA:	SeAg_B0077

SENS:

Q786_00345

SED:

SeD_A0076

SEG:	SG0073(caiD)

SEL:	SPUL_0075(caiD)

SEGA:

SPUCDC_0075(caiD)

SET:	SEN0071(caiD)

SENA:

AU38_00345

SENO:

AU37_00345

SENV:

AU39_00345

SENQ:

AU40_00380

SENL:

IY59_00360

SENJ:

CFSAN001992_10675

SEEC:

CFSAN002050_06800

SEEB:

SEEB0189_019035

SEEP:

I137_00325

SENB:

BN855_730(caiD)

SENE:

IA1_00355

SENC:

SEET0819_07240

SES:	SARI_02930

SBG:	SBG_0055(caiD)

SBZ:

A464_58

SBV:	N643_00265

SALZ:

EOS98_18890

SFL:	SF0033(caiD)

SFX:	S0035(caiD)

SFV:	SFV_0030(caiD)

SFE:	SFxv_0034(caiD)

SFN:

SFy_0042

SFS:

SFyv_0046

SFT:	NCTC1_00033(caiD)

SSN:	SSON_0042(caiD)

SDY:	SDY_0058(caiD)

SDZ:	Asd1617_00063

SHQ:	A0259_04020

CRO:	ROD_00381(caiD)

CKO:

CKO_03347

CFD:	CFNIH1_09890

CBRA:

A6J81_13220

CWE:	CO701_18230

CYO:	CD187_20370

CPOT:

FOB25_07465(caiD)

CFQ:	C2U38_03510

CSED:

JY391_18155(caiD)

CAMA:

F384_00195

CAF:	AL524_22270

CIF:	AL515_04985

CFAR:

CI104_04635

CIR:	C2U53_04695

CIE:	AN232_00010

CPAR:

CUC49_00175

CTEL:

GBC03_25660(caiD)

CITZ:

E4Z61_13405(caiD)

CARS:

E1B03_04725(caiD)

EBT:	EBL_c33170(caiD)

ESC:	Entcl_2478

KLE:	AO703_09845

METY:

MRY16398_47870(caiD)

ASUB:

NLZ15_03185(caiD)

YRE:	HEC60_04850(caiD)

KIN:	AB182_21550

PDZ:	HHA33_24115(caiD)

PSGC:

G163CM_28670(caiD)

EBF:

D782_3833

EBC:	C2U52_03245

PMR:	PMI2658(caiD)

PMIB:

BB2000_2649(caiD)

PVL:	AOB99_14525

PVG:	CRN77_06400

PHAU:

PH4a_04765

PROT:

BTA34_14505

PCOL:

F1325_16720(caiD)

PCIB:

F9282_17065(caiD)

PPEE:

I6G31_11885(caiD)

PRG:	RB151_007330(caiD)

PHEI:

NCTC12003_00741(caiD)

PRQ:	CYG50_11980(caiD)

EIC:	NT01EI_2956

ETR:	ETAE_2660(caiD)

ETD:

ETAF_2398

ETE:

ETEE_0761

ETC:	ETAC_12780

EDW:	QY76_07195

EDL:	AAZ33_14070(caiD)

EHO:	A9798_03970

HAV:	AT03_19285

HPAR:

AL518_07310

OPO:	DSM2777_06100

LPV:	HYN51_05060

PRAG:

EKN56_12475(caiD)

LRI:	NCTC12151_02501(caiD)

SLO:

Shew_2672

SSE:

Ssed_3225

SPL:

Spea_4034

SHL:

Shal_0224

SWP:

swp_4934

SALG:

BS332_05375

SLJ:	EGC82_18060

SCAA:

TUM17387_35790(caiD)

SCHK:

GII14_20250(caiD)

NAX:	HC341_11765(caiD)

GAI:	IMCC3135_12755(caiD)

MRZ:	KDW95_01220(caiD) KDW95_01240(caiD)

BCH:	Bcen2424_0177

BXE:

Bxe_C0322

VEI:

Veis_3503

MLO:

mlr5044

MLN:	A9174_02185

MCI:	Mesci_0427

MCIC:

A4R28_26230

MOP:	Mesop_0439

MAM:	Mesau_00647

MAMO:

A6B35_06080

MESW:

A9K65_002145

MESM:

EJ066_09365

MESP:

C1M53_02975

MHUA:

MCHK_6109(caiD)

MJR:	EB229_02220

MERD:

EB233_02335

MESR:

FGU64_17225(caiD)

MOH:	IHQ72_01600(caiD)

MESO:

BSQ44_22625

HOE:	IMCC20628_02948

AAK:	AA2016_1883

AMIH:

CO731_02018(caiD)

PHT:	BLM14_08020

PHYL:

HB779_13100(caiD)

NIY:	FQ775_14120(caiD)

KMN:	HW532_09375(caiD)

SME:	SMc01641(caiD)

SMK:	Sinme_2233

SMQ:	SinmeB_2077

SMX:	SM11_chr1006(caiD)

SMI:	BN406_02077(caiD)

SMEG:

C770_GR4Chr2312

SMEL:

SM2011_c01641(caiD)

SMER:

DU99_12620

SMD:

Smed_2177

RHI:	NGR_c21610

SFH:	SFHH103_02057(caiD)

SFD:	USDA257_c45520(caiD)

SIX:

BSY16_546

SAME:

SAMCFNEI73_Ch2523(caiD)

SINO:

SS05631_c23110

EAD:

OV14_3675

EAH:	FA04_11405

ESJ:	SJ05684_c23160

EAK:	EKH55_2240

EMX:	FKV68_14635(caiD)

ARA:

Arad_9264

RET:	RHE_PC00129

REL:	REMIM1_PB00143(caiD)

REP:	IE4803_PD00564(caiD)

REI:	IE4771_PE00579(caiD)

RLE:	pRL100378 pRL110025(caiD)

RLT:	Rleg2_4496

RLG:

Rleg_6427

RLB:	RLEG3_02200

RLU:	RLEG12_05430

RTR:	RTCIAT899_PC04720

RGA:	RGR602_PC01930(caiD)

RHN:	AMJ98_PB00186(caiD)

RPHA:

AMC79_PA00228(caiD)

RHX:	AMK02_PB00200(caiD)

RHK:	Kim5_PD00537(caiD)

REZ:	AMJ99_PA00186(caiD)

RJG:	CCGE525_28950

RHR:	CKA34_20740

RAD:	CO657_34200

RII:	FFM53_035785(caiD)

RHID:

FFM81_022455(caiD)

RBQ:	J2J99_26490(caiD)

RLN:	J0663_29190(caiD)

RBAN:

J2J98_28810(caiD)

RRG:	J3P73_33665(caiD)

RLS:	HB780_06655(caiD)

RAW:	NE851_06410

RSUL:

N2599_20995

RLW:	RlegWSM1455_33810(caiD)

NEN:	NCHU2750_44880(caiD)

SHZ:

shn_12110

SZO:	K8M09_10840(caiD)

SOJ:	K6301_09590(caiD)

OIN:	IAR37_19850(caiD) IAR37_19855(caiD)

BIC:	LMTR13_25655

NOH:	G5V57_30520(caiD)

LAP:	ACP90_08775

LABR:

CHH27_11740

LABP:

FJ695_03415(caiD)

LABT:

FIU93_14330(caiD1)

LAGG:

B0E33_20850

SIL:	SPO2706(caiD-1) SPOA0285(caiD-2)

SIT:	TM1040_2793

RUA:	D1823_20420

RUT:	FIU92_19795(caiD3)

RCON:

K3740_22445(caiD)

JAN:	Jann_3750 Jann_3824

RDE:	RD1_0613(caiD)

RLI:	RLO149_c000760(caiD)

RPON:

G3256_17965(caiD)

PGA:	PGA1_c34200(caiD)

PGL:	PGA2_c32410(caiD)

PGD:

Gal_03487

PHP:	PhaeoP97_03321(caiD)

PPIC:

PhaeoP14_03227(caiD)

PHQ:	D1820_12070

OAT:	OAN307_c01290(caiD)

OAR:	OA238_c47080(caiD)

OTM:	OSB_31140(caiD)

OCT:	FTO60_02315(caiD)

LMD:	METH_20470

LEJ:	ETW24_19425(caiD)

LAQU:

R2C4_18445(caiD)

LEV:	ETW23_19765(caiD)

LCAE:

K3721_17955(caiD)

MALG:

MALG_01859

YPAC:

CEW88_20160

TPRO:

Ga0080559_TMP3876

SUAM:

BOO69_17795

SULZ:

C1J03_22110

SULI:

C1J05_06970 C1J05_19880

SULD:

B5M07_09545

SPOT:

G6548_16195

SMED:

JNX03_13670(caiD)

SINL:

DSM14862_00287(dpgD) DSM14862_02686(caiD)

SPSE:

SULPSESMR1_03035(caiD)

TOM:	BWR18_00715

RID:	RIdsm_00642(caiD_1) RIdsm_04076(caiD_5)

ROM:	EI983_04465 EI983_14380

ROH:	FIU89_03985(caiD)

AHT:	ANTHELSMS3_00030(caiD)

THAA:

CFI11_00810

MALU:

KU6B_41080(caiD)

PAMI:

JCM7686_pAMI6p079

PKD:	F8A10_13515(caiD)

PPAN:

ESD82_07895

PMEH:

JWJ88_12580(caiD)

PSAP:

JHX88_07315(caiD)

PTP:	RCA23_c04470(caiD)

RMB:	K529_013695

DON:	BSK21_10365

RBZ:	B9057_03240

LVS:	LOKVESSMR4R_02271(caiD)

GFU:	KM031_05375(caiD)

SALO:

EF888_13845

SEDI:

EBB79_11295 EBB79_22480

PSEB:

EOK75_06680(caiD) EOK75_14575(caiD)

PAED:

G5B38_05425(caiD) G5B38_10250(caiD)

MON:	G8E03_13250

PAMO:

BAR1_01805

PALW:

PSAL_036230(caiD)

CACT:

HZ995_05560(caiD)

THAS:

C6Y53_14720(caiD)

HDH:	G5B40_03925(caiD)

GCE:	KYE46_03995(caiD)

NSM:	JO391_19410(caiD)

ACRO:

K3J57_14715(caiD)

AALK:

LGT41_0007670

NDR:	HT578_01825

SAL:

Sala_1808

SPHK:

SKP52_16250

SMAG:

AN936_15975

SMAZ:

LH19_02315

STER:

AOA14_11420

SPHQ:

BWQ93_10915

SPHX:

E5675_04315

SPHU:

SPPYR_2860(caiD)

SRAD:

LLW23_06505

SPPH:

KFK14_08130

HTQ:	FRZ44_11750(caiD) FRZ44_19970(caiD) FRZ44_47680

HADH:

FRZ61_11970(caiD) FRZ61_27270(caiD) FRZ61_44380(caiD) FRZ61_45410

NACI:

NUH88_15650

SNEA:

NBZ79_03370

CRX:	CRECT_1855(caiD)

PAUU:

E8A73_006725

BFD:	NCTC4823_03672(caiD)

MPA:	MAP_2645c(echA1)

MAO:

MAP4_1173

MAVI:

RC58_05820

MAVU:

RE97_05815

MAV:	MAV_1277 MAV_2597

MIT:	OCO_11860 OCO_13790

MIA:	OCU_11810 OCU_14260

MID:

MIP_01900

MYO:

OEM_12000

MCHI:

AN480_06995

MIR:	OCQ_11880 OCQ_28700

MMAL:

CKJ54_05755

MMAN:

MMAN_28130 MMAN_41980

MSA:	Mycsm_04989

MUL:	MUL_0972(echA1_1)

MMI:	MMAR_4317(echA1_1)

MMAE:

MMARE11_41310(echA1_1)

MLI:	MULP_04515(echA1_1)

MPSE:

MPSD_44540

MSHO:

MSHO_17550

MMC:

Mmcs_4079

MKM:

Mkms_4155

MJL:	Mjls_4093 Mjls_4309

MMM:

W7S_05790

MKN:	MKAN_07490

MYV:	G155_22570

MYE:	AB431_06615

MDX:	BTO20_08550

MSHG:

MSG_03869

MFJ:	MFLOJ_47970

MSTO:

MSTO_52030

MSIM:

MSIM_37940

MSAK:

MSAS_21500

MKU:	I2456_20315

MLW:	MJO58_21180

MGRO:

FZ046_26260

MXE:	MYXE_14510

MNV:	MNVI_05690

MPAG:

C0J29_23630

MNM:	MNVM_07160

MGOR:

H0P51_21965

MCOO:

MCOO_19830

MBAI:

MB901379_03773(caiD_4)

MSEO:

MSEO_26470 MSEO_30240

MHEK:

JMUB5695_01387 JMUB5695_01931

MGAU:

MGALJ_29570

MLJ:	MLAC_07400

MBRD:

MBRA_27910

MSHJ:

MSHI_03510

MMAM:

K3U93_18710

MSPG:

F6B93_17255

MOT:	LTS72_03700

MPAA:

MKK62_05955

MSM:	MSMEG_1155 MSMEG_5198

MSG:	MSMEI_5063

MSB:	LJ00_25710

MSN:	LI99_25715

MSH:	LI98_25720

MVA:

Mvan_4588

MGI:

Mflv_2117

MSP:	Mspyr1_15440

MCB:	Mycch_4000

MNE:	D174_21215

MYN:	MyAD_20805

MFT:	XA26_46280

MPHL:

MPHLCCUG_04147(caiD_3)

MVQ:

MYVA_4366

MLL:	B1R94_06555

MRH:	MycrhN_3594

MTHN:

4412656_03465(caiD_7)

MHAS:

MHAS_02938(caiD_1)

MDU:	MDUV_01330 MDUV_19620 MDUV_30990

MCHT:

MCHIJ_25860

MDR:	MDOR_28750

MAUU:

NCTC10437_04319(caiD_4)

MMAG:

MMAD_42460

MMOR:

MMOR_19880

MFX:	MFAL_38130

MAIC:

MAIC_43390

MIJ:	MINS_38220

MALV:

MALV_21610 MALV_48800

MTY:	MTOK_04080

MPSC:

MPSYJ_22590

MARZ:

MARA_54590

MGAD:

MGAD_52890

MHEV:

MHEL_09540

MSAR:

MSAR_27290

MANY:

MANY_26590

MAUB:

MAUB_43890

MPOF:

MPOR_46530

MPHU:

MPHO_25530

MMUC:

C1S78_005580

MMAT:

MMAGJ_05020

MBOK:

MBOE_27610 MBOE_59020

MFG:	K6L26_09210

MSEI:

MSEDJ_54650

MFLV:

NCTC10271_01151(caiD_4)

MCEE:

MCEL_44570

MBRM:

L2Z93_000833

MMON:

EWR22_21015 EWR22_22095

MGO:	AFA91_14630

MHOL:

K3U96_06215

MSEN:

K3U95_21655

MPAE:

K0O64_06195

MRF:	MJO55_20740

MDF:	K0O62_22210

MCRO:

MI149_06905

MKR:	MKOR_40760

MPAK:

MIU77_04375

MJD:	JDM601_1118(echA1_1)

MTER:

4434518_01077(echA1_1)

MMIN:

MMIN_27540

MHIB:

MHIB_06500

MHER:

K3U94_03945 K3U94_06205

MVM:	MJO54_06375

NFA:	NFA_29420(echA13)

NFR:	ERS450000_01146(caiD_2)

NOZ:	DMB37_01565

NAD:	NCTC11293_03570(caiD_6)

ROP:

ROP_71000

RCR:	NCTC10994_01638(caiD_5)

RTM:	G4H71_12225

RGO:	KYT97_15210 KYT97_23170

ROZ:	CBI38_34350

RGOR:

NMQ04_04900

GOM:	D7316_02519(dpgD_2)

GJI:	H1R19_06265

GHN:	MVF96_20695

DKN:	NHB83_17000

SCAD:

DN051_01780

YIA:	LO772_01745

LDN:	H9L06_00835

GMN:	GMOLON4_1235(caiD)

ART:

Arth_1079

AAU:

AAur_1078

PNV:	JMY29_05095

NOY:	EXE57_03890(caiD)

NANO:

G5V58_03210(caiD)

NOQ:	LN652_15065

PDX:

Psed_2574

» show all

Reference

1 [PMID:11409545]

Authors

Engemann C, Elssner T, Kleber HP

Title

Biotransformation of crotonobetaine to L(-)-carnitine in Proteus sp.

Journal

Arch Microbiol 175:353-9 (2001)
DOI:10.1007/s002030100272

Sequence

[ag:CAD48582]

Reference

2 [PMID:11551212]

Authors

Elssner T, Engemann C, Baumgart K, Kleber HP

Title

Involvement of coenzyme A esters and two new enzymes, an enoyl-CoA hydratase and a CoA-transferase, in the hydration of crotonobetaine to L-carnitine by Escherichia coli.

Journal

Biochemistry 40:11140-8 (2001)
DOI:10.1021/bi0108812

Sequence

[eco:b0036]

Reference

3 [PMID:15731894]

Authors

Engemann C, Elssner T, Pfeifer S, Krumbholz C, Maier T, Kleber HP

Title

Identification and functional characterisation of genes and corresponding enzymes involved in carnitine metabolism of Proteus sp.

Journal

Arch Microbiol 183:176-89 (2005)
DOI:10.1007/s00203-005-0760-2

Sequence

[ag:CAD48582]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.149

IUBMB Enzyme Nomenclature:

4.2.1.149

ExPASy - ENZYME nomenclature database:

4.2.1.149

BRENDA, the Enzyme Database:

4.2.1.149

DBGET integrated database retrieval system