KEGG   ENZYME: 4.2.1.150
Entry
EC 4.2.1.150                Enzyme                                 

Name
short-chain-enoyl-CoA hydratase;
3-hydroxybutyryl-CoA dehydratase;
crotonase;
crt (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
short-chain-(3S)-3-hydroxyacyl-CoA hydro-lyase
Reaction(IUBMB)
a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain trans-2-enoyl-CoA + H2O
Substrate
short-chain (3S)-3-hydroxyacyl-CoA
Product
short-chain trans-2-enoyl-CoA;
H2O [CPD:C00001]
Comment
The enzyme from the bacterium Clostridium acetobutylicum is part of the central fermentation pathway and plays a key role in the production of both acids and solvents. It is specific for short, C4-C6, chain length substrates and exhibits an extremely high turnover number for crotonyl-CoA. cf. EC 4.2.1.17, enoyl-CoA hydratase and EC 4.2.1.74, long-chain-enoyl-CoA hydratase.
History
EC 4.2.1.150 created 2014
Reference
1  [PMID:5057466]
  Authors
Waterson RM, Castellino FJ, Hass GM, Hill RL
  Title
Purification and characterization of crotonase from Clostridium acetobutylicum.
  Journal
J Biol Chem 247:5266-71 (1972)
Reference
2  [PMID:7024731]
  Authors
Waterson RM, Conway RS
  Title
Enoyl-CoA hydratases from Clostridium acetobutylicum and Escherichia coli.
  Journal
Methods Enzymol 71 Pt C:421-30 (1981)
DOI:10.1016/0076-6879(81)71053-x
Reference
3  [PMID:8655474]
  Authors
Boynton ZL, Bennet GN, Rudolph FB
  Title
Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824.
  Journal
J Bacteriol 178:3015-24 (1996)
DOI:10.1128/JB.178.11.3015-3024.1996
  Sequence
[cac:CA_C2712]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.150
IUBMB Enzyme Nomenclature: 4.2.1.150
ExPASy - ENZYME nomenclature database: 4.2.1.150
BRENDA, the Enzyme Database: 4.2.1.150

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