KEGG ENZYME: 4.2.1.157

ENZYME: 4.2.1.157

Entry

EC 4.2.1.157                Enzyme

Name

(R)-2-hydroxyisocaproyl-CoA dehydratase;
2-hydroxyisocaproyl-CoA dehydratase;
HadBC

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

(R)-2-hydroxy-4-methylpentanoyl-CoA hydro-lyase

Reaction(IUBMB)

(R)-2-hydroxy-4-methylpentanoyl-CoA = 4-methylpent-2-enoyl-CoA + H2O [RN:R11076]

Reaction(KEGG)

R11076

Substrate

(R)-2-hydroxy-4-methylpentanoyl-CoA [CPD:C21089]

Product

4-methylpent-2-enoyl-CoA [CPD:C21090];
H2O [CPD:C00001]

Comment

The enzyme, isolated from the bacterium Peptoclostridium difficile, is involved in the reductive branch of L-leucine fermentation. It catalyses an alpha/beta-dehydration, which depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein HadI.

History

EC 4.2.1.157 created 2015

Orthology

K20025

(R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit

K20026

(R)-2-hydroxyisocaproyl-CoA dehydratase beta subunit

Genes

SYM:	K6K13_14535

PPEE:

I6G31_11955

CBO:	CBO2197(hadC) CBO2198(hadB)

CBA:	CLB_2135(hgdB) CLB_2136

CBH:	CLC_2140 CLC_2141

CBY:	CLM_2398(hgdB) CLM_2399

CBL:	CLK_1636 CLK_1637

CBB:	CLD_2382 CLD_2383

CBI:	CLJ_B2405 CLJ_B2406

CBN:	CbC4_0487(hgdA) CbC4_0488(hgdB)

CBF:	CLI_2241 CLI_2242

CBM:	CBF_2226 CBF_2227

CBJ:	H04402_02206 H04402_02207

CLD:	CLSPO_c22290 CLSPO_c22300

CTYK:

CTK_C06860(fldB) CTK_C06870(fldC)

CTAG:

LL095_07995 LL095_08000

CPRF:

K7H06_13615 K7H06_13620

PBIF:

KXZ80_01470 KXZ80_01475

CFEM:

HCR03_03840

LASA:

L9O85_02215 L9O85_02220

CASP:

NQ535_01535 NQ535_01540

CDF:	CD630_03970(hadB) CD630_03980(hadC)

PDC:	CDIF630_00525(hadB) CDIF630_00526(hadC)

CDC:	CD196_0382(hadB) CD196_0383(hadC)

CDL:	CDR20291_0368(hadB) CDR20291_0369(hadC)

PDF:	CD630DERM_03970(hadB) CD630DERM_03980(hadC)

PSOR:

RSJ16_16040 RSJ16_16045

PEB:	O0R46_02460 O0R46_08740 O0R46_08745

ETM:	CE91St48_25640(hadC) CE91St48_25650(hadB)

AMEI:

LV469_02740 LV469_02745

» show all

Reference

1 [PMID:15654892]

Authors

Kim J, Darley D, Buckel W

Title

2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile.

Journal

FEBS J 272:550-61 (2005)
DOI:10.1111/j.1742-4658.2004.04498.x

Sequence

[pdf:CD630DERM_03970 CD630DERM_03980]

Reference

2 [PMID:21366233]

Authors

Knauer SH, Buckel W, Dobbek H

Title

Structural basis for reductive radical formation and electron recycling in (R)-2-hydroxyisocaproyl-CoA dehydratase.

Journal

J Am Chem Soc 133:4342-7 (2011)
DOI:10.1021/ja1076537

Sequence

[pdf:CD630DERM_03970 CD630DERM_03980]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.157

IUBMB Enzyme Nomenclature:

4.2.1.157

ExPASy - ENZYME nomenclature database:

4.2.1.157

BRENDA, the Enzyme Database:

4.2.1.157

DBGET integrated database retrieval system