KEGG   ENZYME: 4.2.1.157
Entry
EC 4.2.1.157                Enzyme                                 

Name
(R)-2-hydroxyisocaproyl-CoA dehydratase;
2-hydroxyisocaproyl-CoA dehydratase;
HadBC
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(R)-2-hydroxy-4-methylpentanoyl-CoA hydro-lyase
Reaction(IUBMB)
(R)-2-hydroxy-4-methylpentanoyl-CoA = 4-methylpent-2-enoyl-CoA + H2O [RN:R11076]
Reaction(KEGG)
R11076
Substrate
(R)-2-hydroxy-4-methylpentanoyl-CoA [CPD:C21089]
Product
4-methylpent-2-enoyl-CoA [CPD:C21090];
H2O [CPD:C00001]
Comment
The enzyme, isolated from the bacterium Peptoclostridium difficile, is involved in the reductive branch of L-leucine fermentation. It catalyses an alpha/beta-dehydration, which depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein HadI.
History
EC 4.2.1.157 created 2015
Orthology
K20025  (R)-2-hydroxyisocaproyl-CoA dehydratase alpha subunit
K20026  (R)-2-hydroxyisocaproyl-CoA dehydratase beta subunit
Genes
CBO: CBO2197(hadC) CBO2198(hadB)
CBA: CLB_2135(hgdB) CLB_2136
CBH: CLC_2140 CLC_2141
CBY: CLM_2398(hgdB) CLM_2399
CBL: CLK_1636 CLK_1637
CBB: CLD_2382 CLD_2383
CBI: CLJ_B2405 CLJ_B2406
CBN: CbC4_0487(hgdA) CbC4_0488(hgdB)
CBF: CLI_2241 CLI_2242
CBM: CBF_2226 CBF_2227
CTYK: CTK_C06860(fldB) CTK_C06870(fldC)
CDF: CD630_03970(hadB) CD630_03980(hadC)
PDC: CDIF630_00525(hadB) CDIF630_00526(hadC)
CDC: CD196_0382(hadB) CD196_0383(hadC)
CDL: CDR20291_0368(hadB) CDR20291_0369(hadC)
 » show all
Reference
1  [PMID:15654892]
  Authors
Kim J, Darley D, Buckel W
  Title
2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile.
  Journal
FEBS J 272:550-61 (2005)
DOI:10.1111/j.1742-4658.2004.04498.x
  Sequence
Reference
2  [PMID:21366233]
  Authors
Knauer SH, Buckel W, Dobbek H
  Title
Structural basis for reductive radical formation and electron recycling in (R)-2-hydroxyisocaproyl-CoA dehydratase.
  Journal
J Am Chem Soc 133:4342-7 (2011)
DOI:10.1021/ja1076537
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.157
IUBMB Enzyme Nomenclature: 4.2.1.157
ExPASy - ENZYME nomenclature database: 4.2.1.157
BRENDA, the Enzyme Database: 4.2.1.157

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