KEGG   ENZYME: 4.2.1.164
Entry
EC 4.2.1.164                Enzyme                                 
Name
dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase;
SpnQ;
TDP-4-keto-2,6-dideoxy-D-glucose 3-dehydrase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose hydro-lyase (dTDP-2,3,6-trideoxy-alpha-D-hexopyranose-forming)
Reaction(IUBMB)
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R08930]
Reaction(KEGG)
R08930
Substrate
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose [CPD:C11922];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080]
Product
dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose [CPD:C18032];
H2O [CPD:C00001];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
A pyridoxal 5'-phosphate protein. The enzyme, isolated from the bacterium Saccharopolyspora spinosa, participates in the biosynthesis of forosamine. Requires ferredoxin/ferredoxin reductase or flavodoxin/flavodoxin reductase [1].
History
EC 4.2.1.164 created 2016
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13328  dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase
Genes
EGIPZN02_005108
NDRHT578_04935
QGEK3136_03165
AGEAA314_01884
CFEMHCR03_07270(rfbH)
NBRO3I_015225
NIEKV110_14265(rfbH)
SANLKZO11_24440(rfbH) KZO11_29540(rfbH)
SCYER2B67_24825(rfbH)
SBHSBI_06871 SBI_08410(nanG3)
SALSSLNWT_0672
SCIB446_13485
SVTSVTN_18825
SLCSL103_12605
SAMBSAM23877_5613(srm24)
SPAVSpa2297_33020
SGSAVL59_46060
SALWCP975_00175(rfbH)
SGMGCM10017557_73660
SALUDC74_6602
SALLSAZ_34325
SNWBBN63_25010
SVUB1H20_29870
SLXSLAV_40180(vioA)
STROSTRMOE7_07485
SFKKY5_2311c
SDXC4B68_09670
SNQCP978_29680(rfbH)
STIRDDW44_27860
SRKFGW37_16315(rfbH)
SGALCP966_28980(rfbH)
SCINCP977_27990(rfbH)
SBROGQF42_07470(rfbH)
SCHFIPT68_29250(rfbH)
SHUNDWB77_05140(colD)
SHARHUT13_24960(rfbH)
SCYGS1361_04975(vioA1) S1361_29910(vioA2)
SCALI6J39_29675(rfbH)
SAUHSU9_032935(rfbH)
SXNIAG42_36075(rfbH)
SHKJ2N69_28815(rfbH)
SANUK7396_33570(rfbH)
SGOBtest1122_15655(rfbH)
SINEKI385_25120(rfbH)
SDECL3078_20660(rfbH)
SCAEIHE65_08915(rfbH)
STUBMMF93_33220(rfbH)
SSPNLXH13_14095(rfbH)
SLONLGI35_31700(rfbH)
STUDSTRTU_006757(rfbH)
SJNRI060_18355(rfbH)
SKGKJK29_16155(rfbH)
SLAIP8A22_08990(rfbH)
SARMDVA86_23875
KISHUT16_23820(rfbH)
STRIC7M71_025910
YIALO772_07325(rfbH)
ABRYNYE86_15415(rfbH) NYE86_16005(rfbH)
NDANdas_4697
NALB005_1455(ddhC)
NEXNE857_19580(rfbH)
NAKEKGD83_01670(rfbH)
SNAHOUQ99_30480(rfbH)
ACTWF7P10_30415(rfbH)
AGRAAGRA3207_007758(rfbH)
AMAZLUW76_46785(rfbH)
SACGFDZ84_24190(rfbH)
AMDAMED_0638 AMED_5420
AMNRAM_03260 RAM_27630
AMMAMES_0636 AMES_5355
AMZB737_0637 B737_5355
AORISD37_34295
APRTMUY14_22480(rfbH)
ACTUActkin_02430(perS12)
CROSN8J89_20450(rfbH)
UMERM788_02745(rfbH)
SAQSare_1264
MICHFJK98_15555(rfbH)
MPRNQ3V37_18335(rfbH)
ACTLL3i22_039280
PLKCIK06_11140
DVCDvina_05930(rfbH) Dvina_31235(rfbH)
DFUDfulv_29035(rfbH)
DAURDaura_29880(rfbH)
DMATDmats_05410(rfbH) Dmats_26270(rfbH)
CCAZCOUCH_16030(rfbH)
DHILH044_07825
 » show all
Reference
1  [PMID:17076492]
  Authors
Hong L, Zhao Z, Liu HW.
  Title
Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis.
  Journal
J Am Chem Soc 128:14262-3 (2006)
DOI:10.1021/ja0649670
Reference
2  [PMID:18345667]
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.164
IUBMB Enzyme Nomenclature: 4.2.1.164
ExPASy - ENZYME nomenclature database: 4.2.1.164
BRENDA, the Enzyme Database: 4.2.1.164

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