KEGG   ENZYME: 4.2.1.164
Entry
EC 4.2.1.164                Enzyme                                 

Name
dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase;
SpnQ;
TDP-4-keto-2,6-dideoxy-D-glucose 3-dehydrase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose hydro-lyase (dTDP-2,3,6-trideoxy-alpha-D-hexopyranose-forming)
Reaction(IUBMB)
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R08930]
Reaction(KEGG)
R08930
Substrate
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose [CPD:C11922];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080]
Product
dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose [CPD:C18032];
H2O [CPD:C00001];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
A pyridoxal 5'-phosphate protein. The enzyme, isolated from the bacterium Saccharopolyspora spinosa, participates in the biosynthesis of forosamine. Requires ferredoxin/ferredoxin reductase or flavodoxin/flavodoxin reductase [1].
History
EC 4.2.1.164 created 2016
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13328  dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase
Genes
AGE: AA314_01884
NBR: O3I_015225
SBH: SBI_06871 SBI_08410(nanG3)
SALS: SLNWT_0672
SCI: B446_13485
SALU: DC74_6602
SALL: SAZ_34325
SVT: SVTN_18825
SLC: SL103_12605
SAMB: SAM23877_5613(srm24)
SLX: SLAV_40180(vioA)
SFK: KY5_2311c
NDA: Ndas_4697
NAL: B005_1455(ddhC)
ACTW: F7P10_30415(rfbH)
SACG: FDZ84_24190(rfbH)
SAQ: Sare_1264
MICH: FJK98_15555(rfbH)
 » show all
Reference
1  [PMID:17076492]
  Authors
Hong L, Zhao Z, Liu HW.
  Title
Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis.
  Journal
J Am Chem Soc 128:14262-3 (2006)
DOI:10.1021/ja0649670
Reference
2  [PMID:18345667]
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW.
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.164
IUBMB Enzyme Nomenclature: 4.2.1.164
ExPASy - ENZYME nomenclature database: 4.2.1.164
BRENDA, the Enzyme Database: 4.2.1.164

DBGET integrated database retrieval system