KEGG ENZYME: 4.2.1.164

ENZYME: 4.2.1.164

Entry

EC 4.2.1.164                Enzyme

Name

dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase;
SpnQ;
TDP-4-keto-2,6-dideoxy-D-glucose 3-dehydrase

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose hydro-lyase (dTDP-2,3,6-trideoxy-alpha-D-hexopyranose-forming)

Reaction(IUBMB)

dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R08930]

Reaction(KEGG)

R08930

Substrate

dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose [CPD:C11922];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080]

Product

dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose [CPD:C18032];
H2O [CPD:C00001];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]

Comment

A pyridoxal 5'-phosphate protein. The enzyme, isolated from the bacterium Saccharopolyspora spinosa, participates in the biosynthesis of forosamine. Requires ferredoxin/ferredoxin reductase or flavodoxin/flavodoxin reductase [1].

History

EC 4.2.1.164 created 2016

Pathway

ec00523

Polyketide sugar unit biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K13328

dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase

Genes

NDR:	HT578_04935

AGE:	AA314_01884

CFEM:

HCR03_07270(rfbH)

NBR:	O3I_015225

NIE:	KV110_14265(rfbH)

SBH:	SBI_06871 SBI_08410(nanG3)

SALS:

SLNWT_0672

SCI:	B446_13485

SVT:	SVTN_18825

SLC:	SL103_12605

SAMB:

SAM23877_5613(srm24)

SPAV:

Spa2297_33020

SGS:	AVL59_46060

SALW:

CP975_00175(rfbH)

SGM:	GCM10017557_73660

SALU:

DC74_6602

SALL:

SAZ_34325

SNW:	BBN63_25010

SVU:	B1H20_29870

SLX:	SLAV_40180(vioA)

STRO:

STRMOE7_07485

SFK:

KY5_2311c

SDX:	C4B68_09670

SNQ:	CP978_29680(rfbH)

STIR:

DDW44_27860

SRK:	FGW37_16315(rfbH)

SGAL:

CP966_28980(rfbH)

SCIN:

CP977_27990(rfbH)

SBRO:

GQF42_07470(rfbH)

SCHF:

IPT68_29250(rfbH)

SHUN:

DWB77_05140(colD)

SHAR:

HUT13_24960(rfbH)

SCYG:

S1361_04975(vioA1) S1361_29910(vioA2)

SCAL:

I6J39_29675(rfbH)

SAUH:

SU9_032935(rfbH)

SXN:	IAG42_36075(rfbH)

SHK:	J2N69_28815(rfbH)

SANU:

K7396_33570(rfbH)

SGOB:

test1122_15655(rfbH)

SINE:

KI385_25120(rfbH)

SDEC:

L3078_20660(rfbH)

KIS:	HUT16_23820(rfbH)

STRI:

C7M71_025910

YIA:	LO772_07325(rfbH)

ABRY:

NYE86_15415(rfbH) NYE86_16005(rfbH)

NDA:

Ndas_4697

NAL:	B005_1455(ddhC)

NEX:	NE857_19580(rfbH)

SNAH:

OUQ99_30480(rfbH)

ACTW:

F7P10_30415(rfbH)

AGRA:

AGRA3207_007758(rfbH)

SACG:

FDZ84_24190(rfbH)

AMD:	AMED_0638 AMED_5420

AMN:	RAM_03260 RAM_27630

AMM:	AMES_0636 AMES_5355

AMZ:	B737_0637 B737_5355

AORI:

SD37_34295

APRT:

MUY14_22480(rfbH)

ACTU:

Actkin_02430(perS12)

SAQ:

Sare_1264

MICH:

FJK98_15555(rfbH)

PLK:	CIK06_11140

DVC:	Dvina_05930(rfbH) Dvina_31235(rfbH)

DFU:	Dfulv_29035(rfbH)

DAUR:

Daura_29880(rfbH)

DMAT:

Dmats_05410(rfbH) Dmats_26270(rfbH)

CCAZ:

COUCH_16030(rfbH)

» show all

Reference

1 [PMID:17076492]

Authors

Hong L, Zhao Z, Liu HW.

Title

Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis.

Journal

J Am Chem Soc 128:14262-3 (2006)
DOI:10.1021/ja0649670

Reference

2 [PMID:18345667]

Authors

Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW

Title

In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.

Journal

J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383

Other DBs

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