KEGG   ENZYME: 4.2.1.165
Entry
EC 4.2.1.165                Enzyme                                 

Name
chlorophyllide a 31-hydratase;
bchF (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
chlorophyllide-a 31-hydro-lyase
Reaction(IUBMB)
(1) 3-devinyl-3-(1-hydroxyethyl)chlorophyllide a = chlorophyllide a + H2O [RN:R09059];
(2) 3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a = 3-deacetyl-3-vinylbacteriochlorophyllide a + H2O [RN:R09054]
Reaction(KEGG)
R09054 R09059
Substrate
3-devinyl-3-(1-hydroxyethyl)chlorophyllide a [CPD:C18154];
3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a [CPD:C18153]
Product
chlorophyllide a [CPD:C02139];
H2O [CPD:C00001];
3-deacetyl-3-vinylbacteriochlorophyllide a [CPD:C18152]
Comment
The enzyme, together with EC 1.3.7.15, chlorophyllide-a reductase, and EC 1.1.1.396, bacteriochlorophyllide-a dehydrogenase, is involved in the conversion of chlorophyllide a to bacteriochlorophyllide a. The enzymes can act in multiple orders, resulting in the formation of different intermediates, but the final product of the cumulative action of the three enzymes is always bacteriochlorophyllide a. The enzyme catalyses the hydration of a vinyl group on ring A, converting it to a hydroxyethyl group.
History
EC 4.2.1.165 created 2016
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01110  Biosynthesis of secondary metabolites
Orthology
K11336  3-vinyl bacteriochlorophyllide hydratase
Genes
ALV: Alvin_2643
TVI: Thivi_2411
TMB: Thimo_3026
MPUR: MARPU_13965
TSY: THSYN_31440
TTP: E6P07_13140(bchF)
HHA: Hhal_1638
HHK: HH1059_06050
HHC: M911_08235
EBS: ECTOBSL9_2976
HYR: BSY239_1283(bchF)
METP: C1M51_03465(bchF)
RGE: RGE_33450(bchF)
METR: BSY238_2468(bchF)
RPOD: E0E05_11905(bchF)
AGC: BSY240_3645(bchF)
ABAW: D5400_05050(bchF)
BRA: BRADO1638(bchF)
BBT: BBta_6418(bchF)
BRS: S23_19370(bchF)
AOL: S58_59000
BRO: BRAD285_5763(bchF)
BRQ: CIT40_23845(bchF)
BGZ: XH91_02735(bchF)
BBET: F8237_24770(bchF)
RPA: RPA1541(bchF)
RPB: RPB_3982
RPC: RPC_1312
RPD: RPD_3737
RPE: RPE_1346
RPT: Rpal_1729
BOS: BSY19_4034(bchF)
MEA: Mex_1p5274(bchF)
MDI: METDI5874(bchF)
MEX: Mext_4817
MCH: Mchl_5284
MPO: Mpop_5362
MET: M446_3717
MOR: MOC_2253(bchF)
META: Y590_24615
MEE: DA075_04045(bchF)
METD: C0214_26355(bchF)
METS: DK389_01075(bchF)
METI: DK427_18030(bchF)
MMES: MMSR116_22245(bchF)
MTEA: DK419_05005(bchF)
MSL: Msil_2043
BBAR: RHAL1_00839(bchF)
RVA: Rvan_0999
BVR: BVIR_569
BLAG: BLTE_02050(bchF)
MROS: EHO51_17165(bchF)
RSP: RSP_0284(bchF)
RCP: RCAP_rcc00666(bchF)
JAN: Jann_0162(bchF)
RDE: RD1_0140(bchF)
RLI: RLO149_p940700(bchF)
RPON: G3256_18980(bchF)
DSH: Dshi_3533(bchF)
PTP: RCA23_c29930(bchF)
RSU: NHU_00210(bchF)
RHC: RGUI_1987
ROM: EI983_05555(bchF)
RBG: BG454_02925(bchF)
THAA: CFI11_23835(bchF)
GEH: HYN69_05420(bchF)
TAW: EI545_18840(bchF)
FAQ: G5B39_09990(bchF)
MALU: KU6B_16680(bchF)
NOT: C7W88_00555(bchF)
NGF: FRF71_09220(bchF)
NOG: GKE62_08510(bchF)
SPHI: TS85_23010
SSAN: NX02_18330
BLAS: BSY18_2709(bchF)
ERK: CD351_13945(bchF)
ALH: G6N82_11510(bchF)
PHZ: CHX26_14570(bchF)
POT: E2E27_07265(bchF)
AMV: ACMV_18950(bchF)
RRU: Rru_A0624
RRF: F11_03200
RCE: RC1_2116(bchF)
RPM: RSPPHO_00022(bchF)
NCB: C0V82_18050(bchF)
RCA: Rcas_3748
CAU: Caur_0415
CAG: Cagg_3129
CTE: CT1421(bchF)
CPC: Cpar_0743
CCH: Cag_0402
CLI: Clim_0899
PVI: Cvib_1239
PLT: Plut_1421
PPH: Ppha_0981
PAA: Paes_1533
PROS: CHL67_03610(bchF)
 » show all
Reference
1  [PMID:1080053]
  Authors
Pudek MR, Richards WR
  Title
A possible alternate pathway of bacteriochlorophyll biosynthesis in a mutant of Rhodopseudomonas sphaeroides.
  Journal
Biochemistry 14:3132-7 (1975)
DOI:10.1021/bi00685a015
Reference
2  [PMID:8385667]
  Authors
Burke DH, Alberti M, Hearst JE
  Title
bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants.
  Journal
J Bacteriol 175:2414-22 (1993)
DOI:10.1128/JB.175.8.2414-2422.1993
  Sequence
Reference
3  [PMID:26088139]
  Authors
Lange C, Kiesel S, Peters S, Virus S, Scheer H, Jahn D, Moser J
  Title
Broadened Substrate Specificity of 3-Hydroxyethyl Bacteriochlorophyllide a Dehydrogenase (BchC) Indicates a New Route for the Biosynthesis of Bacteriochlorophyll a.
  Journal
J Biol Chem 290:19697-709 (2015)
DOI:10.1074/jbc.M115.660555
  Sequence
[cte:CT1421]
Reference
4  [PMID:26331578]
  Authors
Harada J, Teramura M, Mizoguchi T, Tsukatani Y, Yamamoto K, Tamiaki H
  Title
Stereochemical conversion of C3-vinyl group to 1-hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV: adaptation of green sulfur bacteria to limited-light environments.
  Journal
Mol Microbiol 98:1184-98 (2015)
DOI:10.1111/mmi.13208
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.165
IUBMB Enzyme Nomenclature: 4.2.1.165
ExPASy - ENZYME nomenclature database: 4.2.1.165
BRENDA, the Enzyme Database: 4.2.1.165

DBGET integrated database retrieval system