KEGG   ENZYME: 4.2.1.167
Entry
EC 4.2.1.167                Enzyme                                 

Name
(R)-2-hydroxyglutaryl-CoA dehydratase;
hgdAB (gene names)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(R)-2-hydroxyglutaryl-CoA hydro-lyase ((E)-glutaconyl-CoA-forming)
Reaction(IUBMB)
(R)-2-hydroxyglutaryl-CoA = (E)-glutaconyl-CoA + H2O
Substrate
(R)-2-hydroxyglutaryl-CoA
Product
(E)-glutaconyl-CoA [CPD:C02411];
H2O [CPD:C00001]
Comment
The enzymes from the bacteria Acidaminococcus fermentans and Clostridium symbiosum are involved in the fermentation of L-glutamate. The enzyme contains [4F-4S] clusters, FMNH2 and riboflavin. It must be activated by an activator protein. Once activated, it can catalyse many turnovers.
History
EC 4.2.1.167 created 2016
Orthology
K20903  (R)-2-hydroxyglutaryl-CoA dehydratase subunit alpha
K20904  (R)-2-hydroxyglutaryl-CoA dehydratase subunit beta
Genes
EAL: EAKF1_ch2943
PMIB: BB2000_2634 BB2000_2635
PVL: AOB99_14455 AOB99_14460
PVG: CRN77_06465 CRN77_06470
PHAU: PH4a_04695 PH4a_04700
PROT: BTA34_14440
PCOL: F1325_16655
CLO: HMPREF0868_0586(fldB) HMPREF0868_0587
CAPR: EQM14_01980 EQM14_01985 EQM14_12230 EQM14_12235
CSH: Closa_3010 Closa_3011
OVA: OBV_10880(hgdA) OBV_10890(hgdB)
PED: ING2D1G_1419(hgdB) ING2D1G_1420(hgdA)
PHAR: NCTC13077_00178(hgdA) NCTC13077_00179(hgdB)
 » show all
Reference
1  [PMID:7398622]
  Authors
Buckel W
  Title
The reversible dehydration of (R)-2-hydroxyglutarate to (E)-glutaconate.
  Journal
Eur J Biochem 106:439-47 (1980)
DOI:10.1111/j.1432-1033.1980.tb04590.x
Reference
2  [PMID:3691501]
  Authors
Schweiger G, Dutscho R, Buckel W
  Title
Purification of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. An iron-sulfur protein.
  Journal
Eur J Biochem 169:441-8 (1987)
DOI:10.1111/j.1432-1033.1987.tb13631.x
  Sequence
Reference
3  [PMID:7607244]
  Authors
Muller U, Buckel W
  Title
Activation of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans.
  Journal
Eur J Biochem 230:698-704 (1995)
DOI:10.1111/j.1432-1033.1995.0698h.x
Reference
4  [PMID:10491198]
  Authors
Hans M, Sievers J, Muller U, Bill E, Vorholt JA, Linder D, Buckel W
  Title
2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum.
  Journal
Eur J Biochem 265:404-14 (1999)
DOI:10.1046/j.1432-1327.1999.00748.x
  Sequence
Reference
5  [PMID:11243821]
  Authors
Locher KP, Hans M, Yeh AP, Schmid B, Buckel W, Rees DC
  Title
Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A.
  Journal
J Mol Biol 307:297-308 (2001)
DOI:10.1006/jmbi.2000.4496
Reference
6  [PMID:21434666]
  Authors
Parthasarathy A, Pierik AJ, Kahnt J, Zelder O, Buckel W
  Title
Substrate specificity of 2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum: toward a bio-based production of adipic acid.
  Journal
Biochemistry 50:3540-50 (2011)
DOI:10.1021/bi1020056
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.167
IUBMB Enzyme Nomenclature: 4.2.1.167
ExPASy - ENZYME nomenclature database: 4.2.1.167
BRENDA, the Enzyme Database: 4.2.1.167

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