KEGG   ENZYME: 4.2.1.36
Entry
EC 4.2.1.36                 Enzyme                                 

Name
homoaconitate hydratase;
homoaconitase;
cis-homoaconitase;
HACN;
Lys4;
LysF;
2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase (incorrect)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(Z)-but-1-ene-1,2,4-tricarboxylate-forming]
Reaction(IUBMB)
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:R04371]
Reaction(KEGG)
R04371
Substrate
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:C05662]
Product
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Comment
Requires a [4Fe-4S] cluster for activity. The enzyme from the hyperthermophilic eubacterium Thermus thermophilus can catalyse the reaction shown above but cannot catalyse the previously described reaction, i.e. formation of (R)-homocitrate by hydration of cis-homoaconitate. The enzyme responsible for the conversion of cis-homoaconitate into (R)-homocitrate in T. thermophilus is unknown at present but the reaction can be catalysed in vitro using aconitate hydratase from pig (EC 4.2.1.3) [2].
History
EC 4.2.1.36 created 1972, modified 2007
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K01705  homoaconitate hydratase
Genes
QSU: 112018819 112023082
SCE: YDR234W(LYS4)
AGO: AGOS_ABL106C
ERC: Ecym_5123
KLA: KLLA0_C15125g
KMX: KLMA_30527(LYS4)
LTH: KLTH0E10582g
VPO: Kpol_1031p57
ZRO: ZYRO0A10076g
CGR: CAGL0K10978g
NCS: NCAS_0B02660(NCAS0B02660)
NDI: NDAI_0C05030(NDAI0C05030)
TPF: TPHA_0L01150(TPHA0L01150)
TBL: TBLA_0A01460(TBLA0A01460)
TDL: TDEL_0B01800(TDEL0B01800)
KAF: KAFR_0E02730(KAFR0E02730)
PIC: PICST_88705(LYS4)
SPAA: SPAPADRAFT_58782(LYS4)
CAL: CAALFM_C404410CA(LYS4)
SLB: AWJ20_348(LYS4)
NCR: NCU08898(lys-6)
NTE: NEUTE1DRAFT58933(NEUTE1DRAFT_58933)
MGR: MGG_04842
SSCK: SPSK_03952
CMT: CCM_07793
BFU: BCIN_14g00430(Bclys4)
MBE: MBM_05638
ANI: AN6521.2
ANG: ANI_1_1106134(An15g00350)
ABE: ARB_04155
TVE: TRV_03936
PNO: SNOG_01131(SNOG_01129 SNOG_01130)
PTE: PTT_19520
SPO: SPAC343.16(lys2)
CNE: CNK00580
CNB: CNBK2910
TASA: A1Q1_04925
ABP: AGABI1DRAFT59676(AGABI1DRAFT_59676)
ABV: AGABI2DRAFT184036(AGABI2DRAFT_184036)
MGL: MGL_3298
MRT: MRET_2395
SPAR: SPRG_03993
IAL: IALB_1150
MRO: MROS_2220
CABY: Cabys_582
 » show all
Reference
1  [PMID:5954805]
  Authors
Strassman M, Ceci LN.
  Title
Enzymatic formation of cis-homoaconitic acid, an intermediate in lysine biosynthesis in yeast.
  Journal
J Biol Chem 241:5401-7 (1966)
Reference
2  [PMID:16524361]
  Authors
Jia Y, Tomita T, Yamauchi K, Nishiyama M, Palmer DR.
  Title
Kinetics and product analysis of the reaction catalysed by recombinant homoaconitase from Thermus thermophilus.
  Journal
Biochem J 396:479-85 (2006)
DOI:10.1042/BJ20051711
Reference
3  [PMID:10714900]
  Authors
Zabriskie TM, Jackson MD.
  Title
Lysine biosynthesis and metabolism in fungi.
  Journal
Nat Prod Rep 17:85-97 (2000)
DOI:10.1039/A801345D
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.36
IUBMB Enzyme Nomenclature: 4.2.1.36
ExPASy - ENZYME nomenclature database: 4.2.1.36
BRENDA, the Enzyme Database: 4.2.1.36
CAS: 9030-68-6

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