| Entry |
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| Name |
ATP-dependent NAD(P)H-hydrate dehydratase;
reduced nicotinamide adenine dinucleotide hydrate dehydratase;
ATP-dependent H4NAD(P)+OH dehydratase;
(6S)-beta-6-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase(ATP-hydrolysing);
(6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase (ATP-hydrolysing;
NADH-forming)
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| Class |
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
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| Sysname |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase (ATP-hydrolysing; NADH-forming)
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| Reaction(IUBMB) |
(1) ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH [RN: R00129];
(2) ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH [RN: R10288]
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| Reaction(KEGG) |
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| Substrate |
ATP [CPD: C00002];
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide [CPD: C04856];
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate [CPD: C04899]
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| Product |
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| Comment |
Acts equally well on hydrated NADH and hydrated NADPH. NAD(P)H spontaneously hydrates to both the (6S)- and (6R)- isomers, and these are interconverted by EC 5.1.99.6, NAD(P)H-hydrate epimerase, to a 60:40 ratio [4]. Hence EC 4.2.1.93 together with EC 5.1.99.6 can restore the mixture of hydrates into NAD(P)H [3,4]. The enzyme from eukaryotes has no activity with ADP, contrary to the enzyme from bacteria (cf. EC 4.2.1.136, ADP-dependent NAD(P)H-hydrate dehydratase) [4].
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| History |
EC 4.2.1.93 created 1992, modified 2012
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| Orthology |
| K17757 | ATP-dependent NAD(P)H-hydrate dehydratase |
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| Genes |
» show all
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| Reference |
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| Authors |
MEINHART JO, CHAYKIN S, KREBS EG. |
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| Title |
Enzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide. |
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| Journal |
J Biol Chem 220:821-9 (1956) |
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| Sequence |
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| Reference |
2 |
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| Authors |
Regueiro Varela, B., Amelunxen, R. and Grisolia, S. |
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| Title |
Synthesis and degradation of monohydroxytetrahydronicotinamide adenine dinucleotide phosphate. |
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| Journal |
Physiol Chem Phys 2:445-454 (1970) |
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| Reference |
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| Authors |
Acheson SA, Kirkman HN, Wolfenden R. |
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| Title |
Equilibrium of 5,6-hydration of NADH and mechanism of ATP-dependent dehydration. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Marbaix AY, Noel G, Detroux AM, Vertommen D, Van Schaftingen E, Linster CL |
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| Title |
Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 4.2.1.93 |
| ExPASy - ENZYME nomenclature database: | 4.2.1.93 |
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