 | | ENZYME: 4.2.1.99 | |
| Entry |
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| Name |
2-methylisocitrate dehydratase;
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase
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| Class |
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
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| Sysname |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]
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| Reaction(IUBMB) |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O [RN: R04425]
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| Reaction(KEGG) |
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| Substrate |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate [CPD: C04593]
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| Product |
(Z)-but-2-ene-1,2,3-tricarboxylate [CPD: C04225];
H2O [CPD: C00001]
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| Comment |
The enzyme from the fungus Yarrowia lipolytica (Saccharomycopsis) does not act on isocitrate.
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| History |
EC 4.2.1.99 created 1999
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| Pathway |
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| Orthology |
| K01682 | aconitate hydratase 2 / 2-methylisocitrate dehydratase |
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| Genes |
| ECOO: | ECRM13514_0120(acnB) |
| ECOH: | ECRM13516_0123(acnB) |
| ECW: | EcE24377A_0120(acnB) |
| EDJ: | ECDH1ME8569_0112(acnB) |
| ECOI: | ECOPMV1_00124(acnB) |
| EFE: | EFER_0139(acnB) EFER_3669 |
| ECLS: | LI67_004795 LI67_014050 |
| ESH: | C1N69_03700(acnB) C1N69_11745(acnB) |
| LNI: | CWR52_00525(acnB) CWR52_08405(acnB) |
| KPNK: | BN49_4216(acnB2) BN49_4522(acnB) |
| KOC: | AB185_25560 AB185_31365 |
| KLW: | DA718_23625(acnB) DA718_28150(acnB) |
| KPAS: | LUW96_11350(acnB) LUW96_17225(acnB) |
| KLC: | K7H21_17635(acnB) K7H21_23320(acnB) |
| REE: | electrica_04215(acnB_2) |
| RON: | TE10_06370 TE10_13750 |
| RTG: | NCTC13098_06162(acnB) |
| CFAR: | CI104_05035(acnB) CI104_19375(acnB) |
| CIE: | AN232_09050(acnB) AN232_25850(acnB) |
| CIX: | M4I31_04330(acnB) M4I31_19555(acnB) |
| CLAP: | NCTC11466_03803(acnB) |
| KOR: | AWR26_12485(acnB) AWR26_20880(acnB) |
| KRD: | A3780_02860 A3780_11625 |
| KIE: | NCTC12125_02913(acnB) |
| AHN: | NCTC12129_00910(acnB) |
| KIN: | AB182_18895 AB182_21120 |
| PDZ: | HHA33_21455(acnB) HHA33_23675(acnB) |
| METY: | MRY16398_42980(acnB) MRY16398_46990 |
| PSHI: | SAMEA2665130_0424(acnB) |
| EBC: | C2U52_02770(acnB) C2U52_30150(acnB) |
| YPG: | YpAngola_A1028(acnB) |
| YPI: | YpsIP31758_3359(acnB) |
| SMW: | SMWW4_v1c41130(acnB) |
| SERA: | Ser39006_006310(acnB) Ser39006_011325(acnB) |
| SERQ: | CWC46_06305(acnB) CWC46_11320(acnB) |
| SFJ: | SAMEA4384070_4124(acnB) |
| SOF: | NCTC11214_02610(acnB) |
| PAQU: | DMB82_0016995(acnB) |
| DDC: | Dd586_2559 Dd586_3503 |
| DZC: | W909_07525 W909_16795 |
| DSO: | A4U42_02615 A4U42_05345 |
| CED: | LH89_16225 LH89_19110 |
| DFN: | CVE23_15435(acnB) CVE23_18700(acnB) |
| DAQ: | DAQ1742_01536 DAQ1742_03785(acnB) |
| DIC: | Dpoa569_000631(acnB) Dpoa569_002333 |
| DLC: | O1Q98_14780(acnB) O1Q98_19185 |
| PROD: | PCO85_13170(acnB) PCO85_19240(acnB) |
| EHD: | ERCIPSTX3056_361(acnB) |
| MINT: | C7M51_00073(acnB_1) C7M51_04148(acnB_2) |
| MTHI: | C7M52_02643(acnB_1) C7M52_02903(acnB_2) |
| TPTY: | NCTC11468_03060(acnB) |
| PHEI: | NCTC12003_01272(acnB) |
| PRJ: | NCTC6933_02731(acnB) |
| LRI: | NCTC12151_02584(acnB) |
| HPIT: | NCTC13334_01721(acnB) |
| PDAG: | 4362423_01146(acnB) |
| MGRA: | A4G16_04400 A4G16_10365 |
| AVT: | NCTC3438_01656(acnB) |
| PAET: | NCTC13378_01198(acnB) |
| XFU: | XFF4834R_chr20260(acnB) |
| XPH: | XppCFBP6546_20205(acnB) |
| XHD: | LMG31886_24240(acnB) |
| VAU: | VANGNB10_cI0501(acnB) |
| VGA: | BSQ33_11730 BSQ33_15295 |
| VSR: | Vspart_00591(acnB_1) Vspart_01393(acnB_2) |
| VPL: | SA104470976_02124(acnB) |
| PAEB: | NCGM1900_4775(acnB) |
| PAEU: | BN889_01913(acnB_2) |
| PRE: | PCA10_27520(acnB) PCA10_36960(acnB) |
| PPUD: | DW66_2174 DW66_2650 |
| PVD: | CFBP1590__3735(acnB) |
| PPRC: | PFLCHA0_c26990(acnB) |
| PMUD: | NCTC8068_02755(acnB) |
| PFW: | PF1751_v1c29180(acnB) |
| PPUU: | PputUW4_02915(acnB) |
| PALK: | PSAKL28_17880 PSAKL28_25850 |
| PUM: | HGP31_14225(acnB) HGP31_16915(acnB) |
| PCAM: | HNE05_05745(acnB) HNE05_09115(acnB) |
| PTRT: | HU722_0016510(acnB) |
| PTW: | TUM18999_20000(acnB) |
| PTAE: | NCTC10697_03191(acnB) |
| PIZ: | LAB08_R24760(acnB) LAB08_R29080(acnB) LAB08_R32180(acnB) |
| ACX: | Achr_23090(acnB) Achr_2760(acnB) |
| MSHE: | MAALD49_15660(acnB) |
| PSYC: | DABAL43B_0906(acnB) |
| MCAT: | MC25239_00411(acnB) |
| MCUN: | NCTC10297_00035(acnB) |
| SCAA: | TUM17387_03920(acnB) |
| CPS: | CPS_4800(acnB) CPS_4802 |
| COM: | CMT41_17115 CMT41_17120 |
| COV: | EKO29_17850(acnB) EKO29_17855 |
| CBER: | B5D82_00540 B5D82_00545(acnB) |
| LSD: | EMK97_10140(acnB) EMK97_10145 |
| THAB: | LP316_00720(acnB) LP316_00725 |
| PSM: | PSM_A0202(acnB) PSM_A1319 |
| PIA: | PI2015_0199 PI2015_1276 |
| PRR: | AT705_03685 AT705_12755 |
| PLZ: | S4054249_01040 S4054249_14330 |
| PSPO: | PSPO_a3013(acnB) PSPO_b1106(acnB) |
| PTD: | PTET_a0210(acnB) PTET_a1515(acnB) |
| PVB: | J5X90_06230(acnB) J5X90_15600 |
| AMC: | MADE_1003930 MADE_1003950 |
| AMH: | I633_03675 I633_03695 |
| AMAA: | amad1_03635 amad1_03655 |
| AMAL: | I607_03520 I607_03550 |
| AMAE: | I876_03730 I876_03760 |
| AMAD: | I636_03625 I636_03645 |
| AMAI: | I635_03600 I635_03620 |
| AMAC: | MASE_03115 MASE_03140 |
| AMB: | AMBAS45_03365 AMBAS45_03395 |
| AMG: | AMEC673_03325 AMEC673_03350 |
| AMK: | AMBLS11_03295 AMBLS11_03325 |
| ALT: | ambt_14830 ambt_14840 |
| AAL: | EP13_03590 EP13_03605 |
| AAUS: | EP12_03700 EP12_03715 |
| ASP: | AOR13_2777 AOR13_2780 |
| ASQ: | AVL57_04855 AVL57_04865 |
| AAW: | AVL56_03630 AVL56_03640 |
| ALR: | DS731_04180(acnB) DS731_08400 |
| ALE: | AV939_03925 AV939_03935 |
| ALZ: | AV940_03770 AV940_03780 |
| SALH: | HMF8227_02441(acnB) |
| MISZ: | MishRS11D_15160(acnB) |
| TIB: | THMIRHAM_10820(acnB) |
| TSE: | THMIRHAS_13860(acnB) |
| GAI: | IMCC3135_24600(acnB_1) IMCC3135_28235(acnB_2) |
| TWAN: | HUF19_09520 HUF19_09560 |
| VCW: | GJQ55_07370 GJQ55_07395(acnB) |
| AEL: | NCTC12917_03755(acnB) |
| ZDF: | AN401_14075 AN401_15900 |
| CDIZ: | CEDIAZO_00327(acnB_1) CEDIAZO_03136(acnB_2) |
| NMM: | NMBM01240149_0609(acnB) |
| NMS: | NMBM01240355_1484(acnB) |
| NMQ: | NMBM04240196_0657(acnB) |
| NMZ: | NMBNZ0533_1545(acnB) |
| NMX: | NMA510612_1984(acnB) |
| NWE: | SAMEA3174300_1480(acnB) |
| NZO: | SAMEA4504057_0545(acnB) |
| NCI: | NCTC10296_02110(acnB) |
| NCZ: | NCTC10294_01037(acnB) |
| NANI: | NCTC12227_00144(acnB) |
| RPF: | Rpic12D_3481 Rpic12D_4613 |
| PDIO: | PDMSB3_1574.1(acnB) |
| PLG: | NCTC10937_03643(acnB) |
| LMIR: | NCTC12852_00387(acnB) |
| BTRM: | SAMEA390648700009(acnB) |
| AXX: | ERS451415_04740(acnB) |
| LIM: | L103DPR2_02393(acnB) |
| LIH: | L63ED372_01912(acnB) |
| MBAC: | BN1209_0530 BN1209_1048(acnB) BN1209_1049 |
| FPHO: | SHINM1_008320(acnB) |
| NEN: | NCHU2750_43220(acnB) |
| LALG: | LentiSH36_01266(acnB) |
| SBIN: | SBA_ch1_18090(acnB) |
| HPO: | HMPREF4655_20820(acnB) |
| HPX: | HMPREF0462_0628(acnB) |
| HSH: | NHP194022_05530(acnB) |
| HPUL: | NCTC13154_00831(acnB) |
| CJV: | MTVDSCj20_0840(acnB) |
| CJD: | JJD26997_0985(acnB) JJD26997_1179(acnB) |
| CFV: | CFVI03293_0762(acnB) |
| CAMP: | CFT03427_0977(acnB) |
| CCOC: | CCON33237_0630(acnB) |
| CHV: | CHELV3228_0929(acnB) |
| CPIN: | CPIN18020_0156(acnB) |
| CAMZ: | CVIC12175_0970(acnB) |
| GBN: | GEOBRER4_28320(acnB) |
| SYY: | SYNGTS_2985(slr0665) |
| SYT: | SYNGTI_2984(slr0665) |
| SYS: | SYNPCCN_2983(slr0665) |
| SYQ: | SYNPCCP_2983(slr0665) |
| SYR: | SynRCC307_2508(acnB) |
| SYX: | SynWH7803_2505(acnB) |
| SYH: | Syncc8109_2737(acnB) |
| SYNW: | SynWH8103_02890(acnB) |
| LET: | O77CONTIG1_00373(acnB) |
| CHON: | NIES4102_05170(acnB) |
| SYP: | SYNPCC7002_A1683(acnB) |
| PAGH: | NIES204_12930(acnB) |
| NSPH: | BDGGKGIB_03149(acnB) |
| STAN: | STA3757_05850(acnB) |
| SDJ: | NCTC13534_01414(acnB) |
| STHA: | NCTC11429_04734(acnB) |
| CGLE: | NCTC11432_03017(acnB) |
| CTAI: | NCTC12078_01987(acnB) |
| PROC: | Ptc2401_01637(acnB) |
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| Reference |
1 |
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| Authors |
Aoki, H., Uchiyama, H., Umetsu, H., Tabuchi, T. |
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| Title |
Isolation of 2-methylisocitrate dehydratase, a new enzyme serving in the methylcitric acid cycle for propionate metabolism, from Yarrowia lipolytica. |
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| Journal |
Biosci Biotechnol Biochem 59:1825-1828 (1995) |
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| Reference |
2 |
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| Authors |
Tabuchi, T., Umetsu, H., Aoki, H., Uchiyama, H. |
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| Title |
Characteristics of 2-methylisocitrate dehydratase, isolated from Yarrowia lipolytica, in comparison to aconitase. |
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| Journal |
Biosci Biotechnol Biochem 59:2013-2017 (1995) |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 4.2.1.99 |
| ExPASy - ENZYME nomenclature database: | 4.2.1.99 |
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