KEGG   ENZYME: 4.2.2.20
Entry
EC 4.2.2.20                 Enzyme                                 

Name
chondroitin-sulfate-ABC endolyase;
chondroitinase (ambiguous);
chondroitin ABC eliminase (ambiguous);
chondroitinase ABC (ambiguous);
chondroitin ABC lyase (ambiguous);
chondroitin sulfate ABC lyase (ambiguous);
ChS ABC lyase (ambiguous);
chondroitin sulfate ABC endoeliminase;
chondroitin sulfate ABC endolyase;
ChS ABC lyase I
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
Sysname
chondroitin-sulfate-ABC endolyase
Reaction(IUBMB)
Endolytic cleavage of (1->4)-beta-galactosaminic bonds between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid to produce a mixture of Delta4-unsaturated oligosaccharides of different sizes that are ultimately degraded to Delta4-unsaturated tetra- and disaccharides
Reaction(KEGG)
(other) R10870 R10871 R10872
Comment
This enzyme degrades a variety of glycosaminoglycans of the chondroitin-sulfate- and dermatan-sulfate type. Chondroitin sulfate, chondroitin-sulfate proteoglycan and dermatan sulfate are the best substrates but the enzyme can also act on hyaluronan at a much lower rate. Keratan sulfate, heparan sulfate and heparin are not substrates. In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(beta1-3)GalNAc(beta1-]n, which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(alpha1-3)GalNAc(beta1-]n of DS. Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source [5]. The related enzyme EC 4.2.2.21, chondroitin-sulfate-ABC exolyase, has the same substrate specificity but removes disaccharide residues from the non-reducing ends of both polymeric chondroitin sulfates and their oligosaccharide fragments produced by EC 4.2.2.20 [4].
History
EC 4.2.2.20 created 2006 (EC 4.2.2.4 created 1972, part-incorporated 2006 (EC 4.2.99.6 created 1965, part incorporated 1976))
Orthology
K08961  chondroitin-sulfate-ABC endolyase/exolyase
Genes
SES: SARI_02380
YPE: YPO0824
YPK: y3211
YPH: YPC_0996
YPA: YPA_0445
YPN: YPN_3027
YPM: YP_2832
YPP: YPDSF_0688
YPG: YpAngola_A3277
YPZ: YPZ3_0887
YPD: YPD4_0844
YPX: YPD8_0842
YPW: CH59_1039
YPV: BZ15_2750
YPL: CH46_92
YPS: YPTB3073
YPO: BZ17_3545
YPY: YPK_0997
YPB: YPTS_3194
YPQ: DJ40_3406
YPU: BZ21_2390
YPR: BZ20_3090
YPC: BZ23_2669
YPF: BZ19_2453
YRU: BD65_2433
PPR: PBPRB0153
ARM: ART_3703
SRO: Sros_2256
AGL: PYTT_0964
PBAS: SMSP2_01417(chonabc_1) SMSP2_01425(chonabc_2)
BTHO: Btheta7330_04950(chonabc_1) Btheta7330_04976(chonabc_2)
BOA: Bovatus_03443(chonabc_1) Bovatus_03480(chonabc_2)
BUN: Bun01g_09420(chonabc)
 » show all
Reference
1  [PMID:5647268]
  Authors
Yamagata T, Saito H, Habuchi O, Suzuki S.
  Title
Purification and properties of bacterial chondroitinases and chondrosulfatases.
  Journal
J Biol Chem 243:1523-35 (1968)
Reference
2  [PMID:4231029]
  Authors
Saito H, Yamagata T, Suzuki S.
  Title
Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates.
  Journal
J Biol Chem 243:1536-42 (1968)
Reference
3  [PMID:5647269]
  Authors
Suzuki S, Saito H, Yamagata T, Anno K, Seno N, Kawai Y, Furuhashi T.
  Title
Formation of three types of disulfated disaccharides from chondroitin sulfates by chondroitinase digestion.
  Journal
J Biol Chem 243:1543-50 (1968)
Reference
4  [PMID:9083041]
  Authors
Hamai A, Hashimoto N, Mochizuki H, Kato F, Makiguchi Y, Horie K, Suzuki S
  Title
Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides.
  Journal
J Biol Chem 272:9123-30 (1997)
DOI:10.1074/jbc.272.14.9123
Reference
5  [PMID:16336265]
  Authors
Huckerby TN, Nieduszynski IA, Giannopoulos M, Weeks SD, Sadler IH, Lauder RM.
  Title
Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides.
  Journal
FEBS J 272:6276-86 (2005)
DOI:10.1111/j.1742-4658.2005.05009.x
Other DBs
ExplorEnz - The Enzyme Database: 4.2.2.20
IUBMB Enzyme Nomenclature: 4.2.2.20
ExPASy - ENZYME nomenclature database: 4.2.2.20
BRENDA, the Enzyme Database: 4.2.2.20
CAS: 9024-13-9

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