KEGG   ENZYME: 4.2.2.21Help
Entry
EC 4.2.2.21                 Enzyme                                 

Name
chondroitin-sulfate-ABC exolyase;
chondroitinase (ambiguous);
chondroitin ABC eliminase (ambiguous);
chondroitinase ABC (ambiguous);
chondroitin ABC lyase (ambiguous);
chondroitin sulfate ABC lyase (ambiguous);
ChS ABC lyase (ambiguous);
chondroitin sulfate ABC exoeliminase;
chondroitin sulfate ABC exolyase;
ChS ABC lyase II
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
chondroitin-sulfate-ABC exolyase
Reaction(IUBMB)
Exolytic removal of Delta4-unsaturated disaccharide residues from the non-reducing ends of both polymeric chondroitin/dermatan sulfates and their oligosaccharide fragments.
Reaction(KEGG)
Comment
This enzyme degrades a variety of glycosaminoglycans of the chondroitin-sulfate- and dermatan-sulfate type. Chondroitin sulfate, chondroitin-sulfate proteoglycan and dermatan sulfate are the best substrates but the enzyme can also act on hyaluronan at a much lower rate. Keratan sulfate, heparan sulfate and heparin are not substrates. The related enzyme EC 4.2.2.20, chondroitin-sulfate-ABC endolyase, has the same substrate specificity but produces a mixture of oligosaccharides of different sizes that are ultimately degraded to tetra- and disaccharides [4]. Both enzymes act by the removal of a relatively acidic C-5 proton of the uronic acid followed by the elimination of a 4-linked hexosamine, resulting in the formation of an unsaturated C4---C5 bond on the hexuronic acid moiety of the products [4,6].
History
EC 4.2.2.21 created 2006 (EC 4.2.2.4 created 1972, part-incorporated 2006 (EC 4.2.99.6 created 1965, part incorporated 1976)), modified 2010
Orthology
K08961  chondroitin-sulfate-ABC endolyase/exolyase
Genes
SES: SARI_02380
YPE: YPO0824
YPK: y3211
YPH: YPC_0996
YPA: YPA_0445
YPN: YPN_3027
YPM: YP_2832
YPP: YPDSF_0688
YPG: YpAngola_A3277
YPZ: YPZ3_0887
YPD: YPD4_0844
YPX: YPD8_0842
YPW: CH59_1039
YPV: BZ15_2750
YPL: CH46_92
YPS: YPTB3073
YPO: BZ17_3545
YPY: YPK_0997
YPB: YPTS_3194
YPQ: DJ40_3406
YPU: BZ21_2390
YPR: BZ20_3090
YPC: BZ23_2669
YPF: BZ19_2453
YRU: BD65_2433
PPR: PBPRB0153
ARM: ART_3703
SRO: Sros_2256
AGL: PYTT_0964
PBAS: SMSP2_01417(chonabc_1) SMSP2_01425(chonabc_2)
BTHO: Btheta7330_04950(chonabc_1) Btheta7330_04976(chonabc_2)
BOA: Bovatus_03443(chonabc_1) Bovatus_03480(chonabc_2)
PHE: Phep_0784
 » show all
Taxonomy
Reference
1  [PMID:5647268]
  Authors
Yamagata T, Saito H, Habuchi O, Suzuki S.
  Title
Purification and properties of bacterial chondroitinases and chondrosulfatases.
  Journal
J Biol Chem 243:1523-35 (1968)
Reference
2  [PMID:4231029]
  Authors
Saito H, Yamagata T, Suzuki S.
  Title
Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates.
  Journal
J Biol Chem 243:1536-42 (1968)
Reference
3  [PMID:5647269]
  Authors
Suzuki S, Saito H, Yamagata T, Anno K, Seno N, Kawai Y, Furuhashi T.
  Title
Formation of three types of disulfated disaccharides from chondroitin sulfates by chondroitinase digestion.
  Journal
J Biol Chem 243:1543-50 (1968)
Reference
4  [PMID:9083041]
  Authors
Hamai A, Hashimoto N, Mochizuki H, Kato F, Makiguchi Y, Horie K, Suzuki S.
  Title
Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides.
  Journal
J Biol Chem 272:9123-30 (1997)
DOI:10.1074/jbc.272.14.9123
Reference
5  [PMID:16336265]
  Authors
Huckerby TN, Nieduszynski IA, Giannopoulos M, Weeks SD, Sadler IH, Lauder RM.
  Title
Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides.
  Journal
FEBS J 272:6276-86 (2005)
DOI:10.1111/j.1742-4658.2005.05009.x
Reference
6  [PMID:18992215]
  Authors
Zhang Z, Park Y, Kemp MM, Zhao W, Im AR, Shaya D, Cygler M, Kim YS, Linhardt RJ
  Title
Liquid chromatography-mass spectrometry to study chondroitin lyase action pattern.
  Journal
Anal Biochem 385:57-64 (2009)
DOI:10.1016/j.ab.2008.10.014
Other DBs
ExplorEnz - The Enzyme Database: 4.2.2.21
IUBMB Enzyme Nomenclature: 4.2.2.21
ExPASy - ENZYME nomenclature database: 4.2.2.21
BRENDA, the Enzyme Database: 4.2.2.21
CAS: 1000607-06-6

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