Entry |
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Name |
cyclooctat-9-en-7-ol synthase;
cotB2
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Class |
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
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Sysname |
geranylgeranyl-diphosphate diphosphate-lyase (cyclooctat-9-en-7-ol-forming)
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Reaction(IUBMB) |
geranylgeranyl diphosphate + H2O = cyclooctat-9-en-7-ol + diphosphate [RN: R10763]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
Requires Mg2+. Isolated from the bacterium Streptomyces melanosporofaciens, where it is part of the biosynthesis of cyclooctatin, a potent inhibitor of lysophospholipase.
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History |
EC 4.2.3.146 created 2014
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Pathway |
ec00999 | Biosynthesis of various secondary metabolites - part 1 |
ec01110 | Biosynthesis of secondary metabolites |
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Orthology |
K18686 | cyclooctat-9-en-7-ol synthase |
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Genes |
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Reference |
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Authors |
Kim SY, Zhao P, Igarashi M, Sawa R, Tomita T, Nishiyama M, Kuzuyama T |
Title |
Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases. |
Journal |
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Sequence |
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Reference |
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Authors |
Zhang X, Shang G, Gu L, Shen Y |
Title |
Crystallization and preliminary X-ray diffraction analysis of the diterpene cyclooctatin synthase (CYC) from Streptomyces sp. LZ35. |
Journal |
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Reference |
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Authors |
Janke R, Gorner C, Hirte M, Bruck T, Loll B |
Title |
The first structure of a bacterial diterpene cyclase: CotB2. |
Journal |
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Reference |
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Authors |
Meguro A, Motoyoshi Y, Teramoto K, Ueda S, Totsuka Y, Ando Y, Tomita T, Kim SY, Kimura T, Igarashi M, Sawa R, Shinada T, Nishiyama M, Kuzuyama T |
Title |
An unusual terpene cyclization mechanism involving a carbon-carbon bond rearrangement. |
Journal |
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Reference |
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Authors |
Tomita T, Kim SY, Teramoto K, Meguro A, Ozaki T, Yoshida A, Motoyoshi Y, Mori N, Ishigami K, Watanabe H, Nishiyama M, Kuzuyama T |
Title |
Structural Insights into the CotB2-Catalyzed Cyclization of Geranylgeranyl Diphosphate to the Diterpene Cyclooctat-9-en-7-ol. |
Journal |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 4.2.3.146 |
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