KEGG   ENZYME: 4.2.3.153
Entry
EC 4.2.3.153                Enzyme                                 
Name
(5-formylfuran-3-yl)methyl phosphate synthase;
mfnB (gene name);
4-HFC-P synthase;
4-(hydroxymethyl)-2-furaldehyde phosphate synthase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
D-glyceraldehyde-3-phosphate phosphate-lyase [D-glyceraldehyde-3-phosphate-adding; (5-formylfuran-3-yl)methyl-phosphate-forming]
Reaction(IUBMB)
2 D-glyceraldehyde 3-phosphate = (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O [RN:R10935]
Reaction(KEGG)
R10935
Substrate
D-glyceraldehyde 3-phosphate [CPD:C00118]
Product
(5-formylfuran-3-yl)methyl phosphate [CPD:C20954];
phosphate [CPD:C00009];
H2O [CPD:C00001]
Comment
The enzyme catalyses the reaction in the direction of producing (5-formylfuran-3-yl)methyl phosphate, an intermediate in the biosynthesis of methanofuran. The sequence of events starts with the removal of a phosphate group, followed by aldol condensation and cyclization. Methanofuran is a carbon-carrier cofactor involved in the first step of the methanogenic reduction of carbon dioxide by methanogenic archaea.
History
EC 4.2.3.153 created 2015 as EC 4.1.99.21, transferred 2015 to EC 4.2.3.153
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
Orthology
K09733  (5-formylfuran-3-yl)methyl phosphate synthase
Genes
MCAMCA1243
METUGNH96_09840
MMTMetme_2177
MDNJT25_017785
MDHAYM39_09595
MKOMKLM6_2030
METLU737_08655
MPADKEF85_08895
MELLIVG45_06390
MAHMEALZ_1631
MBUREQU24_12260
MPSYCEK71_07215
MMAIsS8_3940
MMOBF6R98_17675
MEINmethR_P0787
MOZMoryE10_01380
MISZMishRS11D_06710
MESLKKZ03_10210
MEJQ7A_320
MECQ7C_2000
BLEPAL038_02210
NOCNoc_0020
NHLNhal_0008
NWANwat_0015
NWRE3U44_04070
NTTTAO_0004
TSYTHSYN_32940
NTGNSCAC_0003
SEDSAAY24_01705
CVICV_3961
CVCBKX93_17515
CHRIDK842_11800
CHRBDK843_17235
COXE0W60_31965
BUGBC1001_3966
BGFBC1003_5436
BYIBYI23_B014280
BUEBRPE67_BCDS01250
BUQAC233_22125
BPHBphy_5939 Bphy_6499
BCAIK788_0001563 K788_0007396
PARABTO02_23555
PHSC2L64_21780 C2L64_38215
PTERC2L65_26215 C2L65_34210
BUOBRPE64_BCDS01380
VAPVapar_3073
VPDVAPA_1c31370
MPTMpe_A2618
METPC1M51_15050
LCHLcho_3141
RGERGE_40330
AONDEH84_18960
METRBSY238_70
MFAMfla_1579
MMBMmol_0899
MEHM301_0953
MEIMsip34_1682
MEPMPQ_1682
MBACBN1209_0842
MEUACJ67_07105
MPAUZMTM_14810
SPLBSFPGR_25860
AZAAZKH_p0264
AZDCDA09_11360
THUAC731_004880
THKCCZ27_13215
DDZDSYM_25830
PHTBLM14_23840
RGARGR602_PC01578
XAUXaut_4098
XDIEZH22_22955
SNOSnov_0761
STARG3545_09720
LNEFZC33_09385
ANCGBB76_00800
APRAG3A50_16545
MEAMex_1p1776
MDIMETDI2528
MEXMext_1844
MCHMchl_2179
MPOMpop_1795
MZAB2G69_16880
MRDMrad2831_2723
METM446_5015
MNOMnod_5319
MORMOC_3090
METAY590_08835
MAQUMaq22A_c16390
MPHYMCBMB27_02166
MEEDA075_05625
METDC0214_10590
METXA3862_18140
METSDK389_20720
METIDK427_13920
MMESMMSR116_14055
MTEADK419_10715
MINDmvi_45740
MOGMMB17_18045
MSLMsil_2396
MTUNMTUNDRAET4_2732
MLGCWB41_06090
HDNHden_1466
HDTHYPDE_31313
HMCHYPMC_1616
FILBN1229_v1_0575
FIYBN1229_v1_0577
MCGGL4_1772
METGHT051_10230
MSCBN69_1044
MBRYB1812_11155
MROSEHO51_11405
MHEYH2LOC_018390
MPARF7D14_11885
MTWCQW49_15595
CMETK6K41_27275
PLEOOHA_1_00716
HDIHDIA_2298
AALAIGS74_05730
AALMLUX29_06485
AUBLXB15_00905
GBEGbCGDNIH1_0762
GBHGbCGDNIH2_0762
GBCGbCGDNIH3_0762
GBSGbCGDNIH4_0762
ALIAZOLI_p10712 AZOLI_p10905
ABSAZOBR_p270097
ABQABAZ39_25395
ABFAMK58_22405
AZTTSH58p_01130
AZMDM194_17210
AOZHUE56_08990
FWADCMF_03930
HASHalsa_0951
SCOSCO6440(SC9B5.07)
SGRSGR_6403
SGBWQO_03045
SVLStrvi_4821
SHYSHJG_8696
SHOSHJGH_8456
SRCM271_02150
SMALSMALA_7956
SSOII1A49_43495
SCTSCAT_p0075
SCYSCATT_p16690
SFASfla_6110
SBHSBI_00630
STRPF750_0451
SFISFUL_24
SALUDC74_350
SALLSAZ_02200
SLVSLIV_06225
SVTSVTN_36515
SXISXIM_42080
STRCAA958_00285
SAMBSAM23877_0447
SCZABE83_00510
SRWTUE45_pSRc_0556(TUE45_pSRTUE45c_0556)
STRFASR50_01565
SPAVSpa2297_31870
STRTA8713_30870
STRDNI25_06195
SNWBBN63_01430
SSIAA7J05_34195
SVUB1H20_02645
SLXSLAV_07285
SRJSRO_7133
SGDELQ87_03075
SNKCP967_30675
SPADDVK44_35995
SGFHEP81_07463
SCAVCVT27_32595
SBYH7H31_01860
SFUGCNQ36_01120
SGXH4W23_39200
SBROGQF42_43040
SHARHUT13_17420
SLIAHA039_33340
SDWK7C20_00885
SCALI6J39_02775
SPRACP972_00725
SGJIAG43_03440
SHKJ2N69_35590
SDDD9753_32145
KITCFP65_7289
STRIC7M71_008815
YIALO772_35030
PAUSNCTC13651_00686
CAICaci_2711
RBARB5685
PSLPsta_3306
PIRVN12_12075
RULUC8_03640
RMLFF011L_10800
MFFMFFC18_15900
ROLCA51_14680
AHELQ31a_45930
LCREPla8534_59160
AAGGETAA8_51150
BVOPan97_21590
RLCK227x_16810
LPAVPLANPX_4743
AMUCPan181_23780
PNDPla175_10250
AMOBHG15A2_31080
PLMPlim_1240
PEHSpb1_18980
PBSPlabr_0427
PLSVT03_27110
PLHVT85_19830
FMRFuma_01086
GMRGmarT_37070
GIMF1728_28010
GPNPan110_35360
MRIMal4_42800
SDYNMal52_38460
PLONPla110_23730
GESVT84_08320
GOGC1280_29725
GMSSOIL9_54320
TIMGMBLW1_12120
LRSPX52LOC_00478
FTJFTUN_3137
ULIETAA1_44760
TSPHKIH39_02580
IPAIsop_2173
SACISinac_2321
PBORBSF38_01338
AGVOJF2_24720
AMOAnamo_1256
MOXDAMO_2674
MJAMJ_1099
MFEMefer_0778
MVUMetvu_1271
MFSMFS40622_1222
MIFMetin_0768
MJHJH146_0793
MESGMLAUSG7_0977(mfnB)
MESAMLASG1_0311(mfnB)
MIGMetig_1002
MMPMMP0708
MMQMmarC5_0868
MMXMmarC6_0176
MMZMmarC7_1735
MMDGYY_02715
MMAKMMKA1_07610
MMAOMMOS7_07540
MMADMMJJ_02690
MAEMaeo_0482
MVNMevan_1586
MVOMvol_0288
MOKMetok_1018
METFCFE53_05460
MTHMTH_141
MMGMTBMA_c05910
METCMTCT_0106
MWOMWSIV6_0556(mfnB)
METEtca_00125
METZMETMT2_0523
METKFVF72_01600
MTHMFZP57_02630
METJFZP68_07660
MSTMsp_0430
METBAW729_04945
MRUmru_1209
MSIMsm_1628
MEBAbm4_0760
MMILsm9_1400
MEYETL18_04040
MOLYLM1_0623
MAROMarbSA_00380
MELMetbo_0982
MEWMSWAN_1265
METHMBMB1_0925
MFCBRM9_1097
MFIDSM1535_1751
MCUBMCBB_1210(mfnB)
MSUBBK009_04000
METNBK008_11695
METTCIT01_09980
METOCIT02_01200
MEMEHYG87_10375
MFVMfer_0195
MKAMK1602
AFUAF_2196
AFGAFULGI_00024680
AVEArcve_1618
ASTAsulf_00733
FPLFerp_0372
GACGACE_1910
GAHGAH_00745
MBAMbar_A1101
MBYMSBRM_3356
MBWMSBRW_3621
MBARMSBR2_3309
MBAKMSBR3_3310
MACMA_4436
MMAMM_1114
MMAZMmTuc01_1154
MMJMSMAS_0189
MMACMSMAC_0194
MVCMSVAZ_0170
MEKMSKOL_0164
MLSMSLAZ_2918
METMMSMTP_0219
MEFMSWH1_0488
MEQMSWHS_0497
MSJMSSAC_4117
MSZMSSIH_3653
MSWMSSIT_3708
MTHRMSTHT_1717
MTHEMSTHC_1571
MHORMSHOH_0241
MFZAOB57_007765
MBUMbur_1676
MMETMCMEM_2157
MELOJ7W08_09770
MMHMmah_1977
MHAZBHR79_03745
MEVMetev_1027
MZHMzhil_0382
MPYMpsy_1294
MZIHWN40_02195
MHZMetho_1723
MTPMthe_0348
MCJMCON_2254
MHIMhar_0838
MHUMhun_2631
MRTJKHC33_03460
MLAMlab_1383
MEMMemar_1569
MBGBN140_0783
MEMAMMAB1_1009
MCHKMchiMG62_18810
MPIMpet_2359
MBNMboo_1717
MFOMetfor_1272
MPLMpal_2061
MPDMCP_2988
MEZMtc_0569
RCIRCIX681
MELUMTLP_07540
BARBAOA66_0928
LOKILokiarch_34020
 » show all
Reference
1  [PMID:24977328]
  Authors
Miller D, Wang Y, Xu H, Harich K, White RH
  Title
Biosynthesis of the 5-(Aminomethyl)-3-furanmethanol moiety of methanofuran.
  Journal
Biochemistry 53:4635-47 (2014)
DOI:10.1021/bi500615p
  Sequence
[mja:MJ_1099]
Reference
2  [PMID:25372812]
  Authors
Bobik TA, Morales EJ, Shin A, Cascio D, Sawaya MR, Arbing M, Yeates TO, Rasche ME
  Title
Structure of the methanofuran/methanopterin-biosynthetic enzyme MJ1099 from Methanocaldococcus jannaschii.
  Journal
Acta Crystallogr F Struct Biol Commun 70:1472-9 (2014)
DOI:10.1107/S2053230X1402130X
  Sequence
[mja:MJ_1099]
Reference
3  [PMID:25905665]
  Authors
Wang Y, Jones MK, Xu H, Ray WK, White RH
  Title
Mechanism of the Enzymatic Synthesis of 4-(Hydroxymethyl)-2- furancarboxaldehyde-phosphate (4-HFC-P) from Glyceraldehyde-3-phosphate Catalyzed by 4-HFC-P Synthase.
  Journal
Biochemistry 54:2997-3008 (2015)
DOI:10.1021/acs.biochem.5b00176
  Sequence
[mja:MJ_1099]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.153
IUBMB Enzyme Nomenclature: 4.2.3.153
ExPASy - ENZYME nomenclature database: 4.2.3.153
BRENDA, the Enzyme Database: 4.2.3.153

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