KEGG   ENZYME: 4.2.3.154
Entry
EC 4.2.3.154                Enzyme                                 

Name
demethyl-4-deoxygadusol synthase;
Nos2 (gene name);
Anb2 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
D-sedoheptulose-7-phosphate phosphate-lyase (cyclizing; demethyl-4-deoxygadusol-forming)
Reaction(IUBMB)
D-sedoheptulose 7-phosphate = demethyl-4-deoxygadusol + phosphate + H2O [RN:R11326]
Reaction(KEGG)
R11326
Substrate
D-sedoheptulose 7-phosphate [CPD:C05382]
Product
demethyl-4-deoxygadusol [CPD:C21281];
phosphate [CPD:C00009];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the cyanobacterium Nostoc punctiforme PCC 73102, is involved in the biosynthesis of the sunscreen compound shinorine. It requires a divalent metal ion (Zn2+ or Co2+) and an NAD+ cofactor, which is transiently reduced during the reaction. cf. EC 4.2.3.152, 2-epi-5-epi-valiolone synthase and EC 4.2.3.155, 2-epi-valiolone synthase.
History
EC 4.2.3.154 created 2016
Orthology
K21342  demethyl-4-deoxygadusol synthase
Genes
MGRO: FZ046_13290
MPAG: C0J29_21490
MCOO: MCOO_09320(aroB_2)
MGAU: MGALJ_52870
MCB: Mycch_0151
MAUU: NCTC10437_02576(aroB_2)
MMAG: MMAD_33660(aroB_2)
MTY: MTOK_57150(aroB_2)
MPSC: MPSYJ_07130(aroB_1)
MARZ: MARA_24090(aroB_2)
MSEI: MSEDJ_27540(aroB_1)
RHB: NY08_2748
RFA: A3L23_00895(aroB_1)
RHS: A3Q41_02456(aroB_1)
RHU: A3Q40_02457(aroB_2)
PSEA: WY02_06440
AMI: Amir_4259
MPK: VL20_4964
NSH: GXM_06694
AVA: Ava_3858
CTHE: Chro_0778
 » show all
Reference
1  [PMID:20813918]
  Authors
Balskus EP, Walsh CT
  Title
The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria.
  Journal
Science 329:1653-6 (2010)
DOI:10.1126/science.1193637
  Sequence
[npu:Npun_R5600] [ava:Ava_3858]
Reference
2  [PMID:22741921]
  Authors
Asamizu S, Xie P, Brumsted CJ, Flatt PM, Mahmud T
  Title
Evolutionary divergence of sedoheptulose 7-phosphate cyclases leads to several distinct cyclic products.
  Journal
J Am Chem Soc 134:12219-29 (2012)
DOI:10.1021/ja3041866
  Sequence
[npu:Npun_R5600] [ava:Ava_3858]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.154
IUBMB Enzyme Nomenclature: 4.2.3.154
ExPASy - ENZYME nomenclature database: 4.2.3.154
BRENDA, the Enzyme Database: 4.2.3.154

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