KEGG   ENZYME: 4.2.3.16Help
Entry
EC 4.2.3.16                 Enzyme                                 

Name
(4S)-limonene synthase;
(-)-(4S)-limonene synthase;
4S-(-)-limonene synthase;
geranyldiphosphate diphosphate lyase (limonene forming);
geranyldiphosphate diphosphate lyase [cyclizing, (4S)-limonene-forming];
geranyl-diphosphate diphosphate-lyase [cyclizing;
(-)-(4S)-limonene-forming]
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
geranyl-diphosphate diphosphate-lyase [cyclizing; (S)-limonene-forming]
Reaction(IUBMB)
geranyl diphosphate = (S)-limonene + diphosphate [RN:R02013]
Reaction(KEGG)
Substrate
geranyl diphosphate [CPD:C00341]
Product
(S)-limonene;
diphosphate [CPD:C00013]
Comment
A recombinant enzyme (also known as a monoterpene synthase or cyclase) from the grand fir (Abies grandis) requires Mn2+ and K+ for activity. Mg2+ is essentially ineffective as the divalent metal ion cofactor.
History
EC 4.2.3.16 created 2000 as EC 4.1.99.10, transferred 2000 to EC 4.2.3.16, modified 2003
Pathway
ec00902  Monoterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15088  (4S)-limonene synthase
K21925  (-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
K21926  (+)-alpha-terpineol/(4S)-limonene synthase
Genes
EGR: 104440568
RCU: 8276874
JCU: 105645324
SLY: 101245212(TPS3)
SPEN: 107012939
SOT: 102591915
Taxonomy
Reference
1  [PMID:9268308]
  Authors
Bohlmann J, Steele CL, Croteau R.
  Title
Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.
  Journal
J Biol Chem 272:21784-92 (1997)
DOI:10.1074/jbc.272.35.21784
  Sequence
Reference
2  [PMID:8226816]
  Authors
Colby SM, Alonso WR, Katahira EJ, McGarvey DJ, Croteau R.
  Title
4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase.
  Journal
J Biol Chem 268:23016-24 (1993)
  Sequence
Reference
3  [PMID:8806736]
  Authors
Yuba A, Yazaki K, Tabata M, Honda G, Croteau R.
  Title
cDNA cloning, characterization, and functional expression of 4S-(-)-limonene synthase from Perilla frutescens.
  Journal
Arch Biochem Biophys 332:280-7 (1996)
DOI:10.1006/abbi.1996.0343
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.16
IUBMB Enzyme Nomenclature: 4.2.3.16
ExPASy - ENZYME nomenclature database: 4.2.3.16
BRENDA, the Enzyme Database: 4.2.3.16
CAS: 110639-20-8

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