KEGG   ENZYME: 4.2.3.165Help
Entry
EC 4.2.3.165                Enzyme                                 

Name
(1R,4R,5S)-(-)-guaia-6,10(14)-diene synthase;
STC5 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [cyclizing, (1R,4R,5S)-(-)-guaia-6,10(14)-diene-forming]
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = (1R,4R,5S)-(-)-guaia-6,10(14)-diene + diphosphate [RN:R11830]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
(1R,4R,5S)-(-)-guaia-6,10(14)-diene [CPD:C21685];
diphosphate [CPD:C00013]
Comment
The original enzyme (STC5) from the fungus Fusarium fujikuroi is inactive because of a critically naturally occuring mutation that leads to an asparagine to lysine exchange in the NSE (Asn-Ser-Glu) triad, a highly conserved motif of type I terpene cyclases. Sequence correction by site-directed mutagenesis (K288N) restores activity.
History
EC 4.2.3.165 created 2017
Reference
1  [PMID:27294564]
  Authors
Burkhardt I, Siemon T, Henrot M, Studt L, Rosler S, Tudzynski B, Christmann M, Dickschat JS
  Title
Mechanistic Characterisation of Two Sesquiterpene Cyclases from the Plant Pathogenic Fungus Fusarium fujikuroi.
  Journal
Angew Chem Int Ed Engl 55:8748-51 (2016)
DOI:10.1002/anie.201603782
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.165
IUBMB Enzyme Nomenclature: 4.2.3.165
ExPASy - ENZYME nomenclature database: 4.2.3.165
BRENDA, the Enzyme Database: 4.2.3.165

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