KEGG ENZYME: 4.2.3.33

ENZYME: 4.2.3.33

Entry

EC 4.2.3.33                 Enzyme

Name

stemar-13-ene synthase;
OsDTC2;
OsK8;
OsKL8;
OsKS8;
stemarene synthase;
syn-stemar-13-ene synthase

Class

Lyases;
Carbon-oxygen lyases;
Acting on phosphates

Sysname

9alpha-copalyl-diphosphate diphosphate-lyase (stemar-13-ene-forming)

Reaction(IUBMB)

9alpha-copalyl diphosphate = stemar-13-ene + diphosphate [RN:R09115]

Reaction(KEGG)

R09115

Substrate

9alpha-copalyl diphosphate [CPD:C11902]

Product

stemar-13-ene [CPD:C18223];
diphosphate [CPD:C00013]

Comment

This diterpene cyclase produces stemar-13-ene, a putative precursor of the rice phytoalexin oryzalexin S. Phytoalexins are diterpenoid secondary metabolites that are involved in the defense mechanism of the plant, and are produced in response to pathogen attack through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation.

History

EC 4.2.3.33 created 2008

Pathway

ec00904

Diterpenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K14044

stemar-13-ene synthase

Genes

OSA:

136354096

OBR:

102711108

Reference

1 [PMID:8651702]

Authors

Mohan RS, Yee NK, Coates RM, Ren YY, Stamenkovic P, Mendez I, West CA.

Title

Biosynthesis of cyclic diterpene hydrocarbons in rice cell suspensions: conversion of 9,10-syn-labda-8(17),13-dienyl diphosphate to 9beta-pimara-7,15-diene and stemar-13-ene.

Journal

Arch Biochem Biophys 330:33-47 (1996)
DOI:10.1006/abbi.1996.0223

Reference

2 [PMID:15280039]

Authors

Nemoto T, Cho EM, Okada A, Okada K, Otomo K, Kanno Y, Toyomasu T, Mitsuhashi W, Sassa T, Minami E, Shibuya N, Nishiyama M, Nojiri H, Yamane H

Title

Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice.

Journal

FEBS Lett 571:182-6 (2004)
DOI:10.1016/j.febslet.2004.07.002

Sequence

[osa:136354096]