KEGG   ENZYME: 4.2.3.96
Entry
EC 4.2.3.96                 Enzyme                                 
Name
avermitilol synthase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
avermitilol hydrolase (cyclizing, avermitilol-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate + H2O = avermitilol + diphosphate [RN:R09896]
Reaction(KEGG)
R09896
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
H2O [CPD:C00001]
Product
avermitilol [CPD:C20181];
diphosphate [CPD:C00013]
Comment
Requires Mg2+. The recombinent enzyme gives avermitilol (85%) plus traces of germacrene A, germacrene B and viridiflorol. The (1S)-hydrogen of farnesyl diphosphate is retained.
History
EC 4.2.3.96 created 2012
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
Orthology
K18110  avermitilol synthase
Genes
PBRU: LZC95_34670
SMA: SAVERM_76(ams)
STRM: M444_02785
SGRS: V9K83_30630
SVN: CP980_01435
SNF: JYK04_00062(tpc1_1)
SMOB: J7W19_05280
SXT: KPP03845_100097(tpc1)
SGK: PET44_01335
SPRF: K4G22_05865
KCH: O1G21_07810
KNB: ACF9IK_06765
STRH: GXP74_03530
 » show all
Reference
1  [PMID:20536237]
  Authors
Chou WK, Fanizza I, Uchiyama T, Komatsu M, Ikeda H, Cane DE
  Title
Genome mining in Streptomyces avermitilis: cloning and characterization of SAV_76, the synthase for a new sesquiterpene, avermitilol.
  Journal
J Am Chem Soc 132:8850-1 (2010)
DOI:10.1021/ja103087w
  Sequence
[sma:SAVERM_76]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.96
IUBMB Enzyme Nomenclature: 4.2.3.96
ExPASy - ENZYME nomenclature database: 4.2.3.96
BRENDA, the Enzyme Database: 4.2.3.96

  All links  
Protein sequence (3)   
   UniProt (2)   
   SWISS-PROT (1)   
DNA sequence (10)   
   GenBank (5)   
   EMBL (5)   
Protein domain (2)   
   InterPro (2)   
All databases (15)   

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