| Entry |
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| Name |
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| Class |
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
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| Sysname |
avermitilol hydrolase (cyclizing, avermitilol-forming)
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| Reaction(IUBMB) |
(2E,6E)-farnesyl diphosphate + H2O = avermitilol + diphosphate [RN: R09896]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Requires Mg2+. The recombinent enzyme gives avermitilol (85%) plus traces of germacrene A, germacrene B and viridiflorol. The (1S)-hydrogen of farnesyl diphosphate is retained.
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| History |
EC 4.2.3.96 created 2012
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| Pathway |
| ec00909 | Sesquiterpenoid and triterpenoid biosynthesis |
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| Orthology |
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| Genes |
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| Reference |
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| Authors |
Chou WK, Fanizza I, Uchiyama T, Komatsu M, Ikeda H, Cane DE |
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| Title |
Genome mining in Streptomyces avermitilis: cloning and characterization of SAV_76, the synthase for a new sesquiterpene, avermitilol. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 4.2.3.96 |
| ExPASy - ENZYME nomenclature database: | 4.2.3.96 |
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