Entry |
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Name |
carbamoyl-serine ammonia-lyase;
O-carbamoyl-L-serine deaminase;
carbamoylserine deaminase;
O-carbamoyl-L-serine ammonia-lyase (pyruvate-forming)
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Class |
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
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Sysname |
O-carbamoyl-L-serine ammonia-lyase (decarboxylating; pyruvate-forming)
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Reaction(IUBMB) |
O-carbamoyl-L-serine + H2O = pyruvate + 2 NH3 + CO2 (overall reaction) [RN: R00213];
(1a) O-carbamoyl-L-serine = CO2 + NH3 + 2-aminoprop-2-enoate;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-oxygen bond, releasing CO2, ammonia, and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and a second ammonia molecule. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase.
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History |
EC 4.3.1.13 created 1976
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Reference |
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Authors |
Copper AJ, Meister A. |
Title |
Enzymatic conversion of O-carbamyl-L-serine to pyruvate and ammonia. |
Journal |
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Other DBs |
ExplorEnz - The Enzyme Database: | 4.3.1.13 |
ExPASy - ENZYME nomenclature database: | 4.3.1.13 |
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