KEGG   ENZYME: 4.3.1.32Help
Entry
EC 4.3.1.32                 Enzyme                                 

Name
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase;
FO synthase;
fbiC (gene name) (ambiguous);
cofG (gene name)
Class
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
BRITE hierarchy
Sysname
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil ammonia-lyase (7,8-didemethyl-8-hydroxy-5-deazariboflavin-forming)
Reaction(IUBMB)
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + NH3 + L-methionine + 5'-deoxyadenosine [RN:R12162]
Reaction(KEGG)
Substrate
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil [CPD:C21971];
S-adenosyl-L-methionine [CPD:C00019]
Product
7,8-didemethyl-8-hydroxy-5-deazariboflavin [CPD:C19154];
NH3 [CPD:C00014];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198]
Comment
The enzyme produces the 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) precursor of the redox cofactor coenzyme F420, which is found in methanogens and in various actinobacteria. FO is also produced by some cyanobacteria and eukaryotes. The enzyme, which forms a complex with EC 2.5.1.147, 5-amino-6-(D-ribitylamino)uracil---L-tyrosine 4-hydroxyphenyl transferase, is a radical SAM enzyme that uses the 5'-deoxyadenosyl radical to catalyse the condensation reaction.
History
EC 4.3.1.32 created 2010 as EC 2.5.1.77, part transferred 2018 to EC 4.3.1.32
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K11779  FO synthase
K11780  7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
Genes
CPS: CPS_4048 CPS_4049
ALT: ambt_18390(cofG) ambt_18395
AAL: EP13_00575 EP13_00580
AAUS: EP12_00555
ASP: AOR13_3368 AOR13_3369
ASQ: AVL57_01940 AVL57_01945
AAW: AVL56_00750 AVL56_00755
ALE: AV939_00755 AV939_00760
ALZ: AV940_00755 AV940_00760
ZAL: AZF00_12035(fbiC)
MAGA: Mag101_11125(fbiC)
SDF: ACG33_00245(fbiC)
PSPW: BJG93_33610(fbiC)
EBA: p2A350
AZD: CDA09_09830(fbiC)
MESP: C1M53_21080(cofH) C1M53_21085(cofG)
BRK: CWS35_20200(fbiC)
RPB: RPB_4423
OCA: OCAR_5007
OCG: OCA5_c29510(fbiC)
SNO: Snov_4230
HMC: HYPMC_3547(fbiC)
RBM: TEF_20090(fbiC)
PZU: PHZ_c2536
PSF: PSE_2792(fbiC) PSE_2793(fbiC)
THAS: C6Y53_09640(cofH) C6Y53_09645(cofG)
NPN: JI59_17370(fbiC)
NRE: BES08_02715(fbiC) BES08_26705(fbiC)
NOV: TQ38_007750(fbiC)
NTD: EGO55_02465(cofH)
SPHO: C3E99_13050(fbiC)
SPHU: SPPYR_1437(fbiC)
SWI: Swit_0717
SPHD: HY78_17635(fbiC) HY78_23110(fbiC)
SPHM: G432_01225(fbiC)
SPHJ: BSL82_04895(fbiC) BSL82_08920(fbiC)
SYB: TZ53_17125(fbiC) TZ53_18465(fbiC)
SBD: ATN00_17480(fbiC)
SPMI: K663_16861(fbiC)
SPHB: EP837_03073(fbiC)
SPHT: K426_12255(fbiC) K426_23844(fbiC)
SHYD: CJD35_15490(fbiC)
SYA: A6768_05840(fbiC)
BLAS: BSY18_2471(cofG)
RDI: CMV14_13670(fbiC) CMV14_16710(cofG)
CMAN: A9D14_14800(fbiC)
BBEV: BBEV_1717(fbiC)
MTU: Rv1173(fbiC)
MTV: RVBD_1173
MTC: MT1210
MRA: MRA_1184(fbiC)
MTG: MRGA327_07395(fbiC)
MTUR: CFBS_1252(fbiC)
MTO: MTCTRI2_1205(fbiC)
MTD: UDA_1173(fbiC)
MTN: ERDMAN_1316(fbiC)
MTJ: J112_06345(fbiC)
MTUE: J114_06345(fbiC)
MTX: M943_06155(fbiC)
MTUL: TBHG_01157
MTUT: HKBT1_1250(fbiC)
MTUU: HKBT2_1256(fbiC)
MTQ: HKBS1_1254(fbiC)
MBO: BQ2027_MB1206(fbiC)
MBB: BCG_1236(fbiC)
MBT: JTY_1209(fbiC)
MBM: BCGMEX_1208(fbiC)
MBX: BCGT_1005
MAF: MAF_11920(fbiC)
MMIC: RN08_1318
MCE: MCAN_11841(fbiC)
MCQ: BN44_11312(fbiC)
MCV: BN43_30241(fbiC)
MCX: BN42_21039(fbiC)
MCZ: BN45_30234(fbiC)
MLE: ML1492(fbiC)
MLB: MLBr01492
MPA: MAP_2611c
MAO: MAP4_1207
MAVI: RC58_05980(fbiC)
MAVU: RE97_05975(fbiC)
MAV: MAV_1312
MIT: OCO_12230
MIA: OCU_12190
MID: MIP_01953
MYO: OEM_12380
MCHI: AN480_07165(fbiC)
MMAL: CKJ54_05940(fbiC)
MLP: MLM_1243
MUL: MUL_1020(fbiC)
MMC: Mmcs_4032
MKM: Mkms_4107
MJL: Mjls_4262
MMI: MMAR_4279(fbiC)
MMAE: MMARE11_40940(fbiC)
MMM: W7S_05970(fbiC)
MLI: MULP_04464(fbiC)
MKN: MKAN_07265(fbiC)
MYV: G155_22255(fbiC)
MYE: AB431_23285(fbiC)
MHAD: B586_07415(fbiC)
MYN: MyAD_20555(fbiC)
MDX: BTO20_08855(fbiC)
MSHG: MSG_03833(fbiC)
MSM: MSMEG_5126(fbiC)
MSG: MSMEI_4998(fbiC)
MSB: LJ00_25350(fbiC)
MSN: LI99_25355(fbiC)
MSH: LI98_25360(fbiC)
MVA: Mvan_4533
MGI: Mflv_2167
MNE: D174_20945(fbiC)
MGO: AFA91_14910(fbiC)
MPHL: MPHLCCUG_04074(mqnE)
MVQ: MYVA_4308(fbiC)
MLL: B1R94_06840(fbiC)
MHAS: MHAS_02879(fbiC)
MAB: MAB_1319
MMV: MYCMA_0695(fbiC)
MABB: MASS_1319(fbiC)
MIZ: BAB75_07105(fbiC)
MSTE: MSTE_01287
MSAO: MYCSP_05810(fbiC)
MJD: JDM601_1161(fbiC)
MTER: 4434518_01112(fbiC)
ASD: AS9A_3366(fbiC)
NFA: NFA_47520
NCY: NOCYR_4499(fbiC)
NBR: O3I_035915(fbiC)
NNO: NONO_c64550(fbiC)
NSL: BOX37_25530(fbiC)
NSR: NS506_01897(fbiC)
NTP: CRH09_33525(fbiC)
RER: RER_41830(fbiC)
REY: O5Y_19555(fbiC)
REB: XU06_19700(fbiC)
ROP: ROP_60060(fbiC)
REQ: REQ_14170
RPY: Y013_22830(fbiC)
RHB: NY08_5017
RAV: AAT18_08315(fbiC)
RFA: A3L23_03076(fbiC)
RHW: BFN03_02640(fbiC)
RHS: A3Q41_00251(fbiC)
RRZ: CS378_23345(fbiC)
RHU: A3Q40_02330(fbiC)
RQI: C1M55_20650(fbiC)
RHQ: IM25_12240(fbiC)
RHOD: AOT96_01090(fbiC)
RBY: CEJ39_09030(fbiC)
GBR: Gbro_3324
GPO: GPOL_c31220(fbiC1)
GOR: KTR9_3363
GOQ: ACH46_05885(fbiC)
GOC: CXX93_08415(fbiC)
GIT: C6V83_14745(fbiC)
GOM: D7316_05361(fbiC_1) D7316_05363(fbiC_2)
GAV: C5O27_10905(cofG)
TPR: Tpau_1148
TSM: ASU32_06460(fbiC)
SRT: Srot_0835
DIT: C3V38_15800(fbiC)
DIZ: CT688_09915(fbiC)
DPC: A6048_10140(fbiC)
DLU: A6035_09660(fbiC)
SCO: SCO4429(fbiC)
SALB: XNR_2222
SMA: SAVERM_3794(cofG)
SGR: SGR_4235
SGB: WQO_14275(fbiC)
SCB: SCAB_52181(fbiC)
SCT: SCAT_2338(fbiC)
SFA: Sfla_3633
SBH: SBI_06333(fbiC)
SHY: SHJG_4710
SVE: SVEN_3084
SDV: BN159_5026(fbiC)
SALS: SLNWT_4334
STRP: F750_3084
SFI: SFUL_2886
SCI: B446_16865(fbiC)
SRC: M271_20760(fbiC) M271_38995(fbiC)
SALU: DC74_3703
SALL: SAZ_19675(fbiC)
SLV: SLIV_16290(fbiC)
SGU: SGLAU_14900(fbiC)
SVT: SVTN_16080(fbiC)
STRE: GZL_05186
SCW: TU94_14370(fbiC)
SLD: T261_4807
SLC: SL103_02830(fbiC)
STRM: M444_15375(fbiC)
STRC: AA958_12800(fbiC)
SAMB: SAM23877_3304(fbiC)
SPRI: SPRI_4317
SCZ: ABE83_21500(fbiC)
SCX: AS200_26105(fbiC)
SRW: TUE45_04323(mqnE_1)
STRF: ASR50_19115(fbiC)
SLE: sle_40200(sle_40200)
SRN: A4G23_02305(mqnE_1)
SPAV: Spa2297_14305(fbiC)
STRT: A8713_13345(fbiC)
SCLF: BB341_11230(fbiC)
SGS: AVL59_44175(fbiC)
STSI: A4E84_17315(fbiC)
SNR: SNOUR_23810(fbiC)
SPLU: LK06_018940(fbiC)
STRD: NI25_22395(fbiC)
SNW: BBN63_19680(fbiC)
SAUO: BV401_26765(fbiC) BV401_35045(fbiC)
SSIA: A7J05_20325(fbiC)
SVU: B1H20_13635(fbiC)
SPUN: BFF78_24080(fbiC)
SGV: B1H19_17935(fbiC)
SLAU: SLA_3135
SALF: SMD44_03660(fbiC)
SALJ: SMD11_3959(fbiC)
SLX: SLAV_16495(mqnE2)
STRO: STRMOE7_16995(fbiC)
SFK: KY5_3501c
SNZ: DC008_14780(fbiC)
KSK: KSE_45370(fbiC)
KAB: B7C62_13575(fbiC)
KAU: B6264_08870(fbiC)
MTS: MTES_1880
MCW: A8L33_02330(fbiC)
MIH: BJP65_10345(fbiC)
RRY: C1O28_02855(cofG)
RIA: C7V51_08340(cofG)
HUM: DVJ78_07475(cofG)
GRY: D7I44_06910(cofG)
LMOI: VV02_14120(fbiC)
ARS: ADJ73_02705(fbiC)
NDK: I601_0314(mqnE)
KFL: Kfla_6490
PSIM: KR76_06880(fbiC)
TFU: Tfu_2519
NDA: Ndas_3771
NAL: B005_0949(cofG)
NGV: CDO52_24260(fbiC)
STRR: EKD16_20105(mqnE)
TCU: Tcur_4075
SRO: Sros_1376
NOA: BKM31_38790(fbiC)
FAL: FRAAL1263
ACE: Acel_0769
NML: Namu_4262
GOB: Gobs_1483
BSD: BLASA_1295(fbiC)
MMAR: MODMU_1466(fbiC)
KRA: Krad_3632
SEN: SACE_6594
AMD: AMED_6904(fbiC)
AMN: RAM_35420(fbiC)
AMM: AMES_6798(fbiC)
AMZ: B737_6798(fbiC)
AOI: AORI_1815(fbiC)
AJA: AJAP_30405(fbiC)
AMQ: AMETH_5642(fbiC)
AMYC: CU254_27985(fbiC)
AMYB: BKN51_01275(fbiC)
AAB: A4R43_34635(fbiC)
PDX: Psed_5307
PSEA: WY02_11900(fbiC)
PSEE: FRP1_02325(fbiC)
PSEH: XF36_01695(fbiC)
PSEQ: AD006_09945(fbiC)
PECQ: AD017_17775(fbiC)
PHH: AFB00_15685(fbiC)
AMI: Amir_6472
APRE: CNX65_31760(fbiC)
SESP: BN6_77580(fbiC)
KAL: KALB_8047
KPHY: AOZ06_48880(fbiC)
LED: BBK82_18465(fbiC)
ACTI: UA75_18405
ACAD: UA74_17915
AHG: AHOG_15900(mqnE)
ACTA: C1701_18075(fbiC)
ALO: CRK57346
PMAD: BAY61_03415(fbiC)
SAQ: Sare_0888
MIL: ML5_1202
MTUA: CSH63_19720(fbiC)
VMA: VAB18032_09475(fbiC)
AMS: AMIS_8560
ASE: ACPL_1050(fbiC)
ACTN: L083_1155(fbiC)
AFS: AFR_05660(fbiC)
ACTS: ACWT_0932
PLAB: C6361_20395(fbiC)
PLAT: C6W10_23365(fbiC)
CAI: Caci_8766
TBI: Tbis_0746
AEY: CDG81_19075(fbiC)
RXY: Rxyl_0975
AFO: Afer_0900
AYM: YM304_13120(fbiC)
SYN: sll1285
SYZ: MYO_16490
SYY: SYNGTS_0642(cofG)
SYT: SYNGTI_0642(cofG)
SYS: SYNPCCN_0642(cofG)
SYQ: SYNPCCP_0642(cofG)
SYC: syc0648_d
TEL: tll1515(cofG)
THN: NK55_04200(cofG)
CYI: CBM981_1919(cofG)
HHG: XM38_019080(cofG)
MAR: MAE_05370
MPK: VL20_1284
CYL: AA637_08445(cofG)
CYT: cce_2717
TER: Tery_0173
ARP: NIES39_K03090(cofG)
PAGH: NIES204_39250(cofG)
GVI: gll2100(cofG)
GLJ: GKIL_0029(cofG)
ANA: alr3573
AVA: Ava_1598
NAZ: Aazo_0829
ANB: ANA_C12049(thiH)
CALH: IJ00_16040
NSP: BMF81_01795(cofG)
CTHE: Chro_4336
TRO: trd_A0899(cofH) trd_A0900(cofG)
KBS: EPA93_00450(cofH)
TTR: Tter_2623
MJA: MJ_0446
MMP: MMP0876(cofG)
MMD: GYY_05090(cofG)
MAE: Maeo_0911
MVO: Mvol_0642
MTH: MTH_1198
MMG: MTBMA_c15760(cofG)
METC: MTCT_1091
MWO: MWSIV6_1591(cofG)
METE: tca_01155(fbiC_2)
MST: Msp_0296(cofG)
METB: AW729_05670(cofG)
MSI: Msm_1035
MRU: mru_1974(cofG)
MEB: Abm4_1547(cofG)
MMIL: sm9_2047(cofG)
MEYE: TL18_09420(cofG)
MOL: YLM1_1412
METH: MBMB1_1721(cofG)
MFC: BRM9_0974(cofG)
MFI: DSM1535_0944(cofG)
MCUB: MCBB_2020(cofG)
METO: CIT02_09285(cofG)
MFV: Mfer_0395
MKA: MK0898(thiH_2)
AFU: AF_0797
FPL: Ferp_0002
GAC: GACE_2058
GAH: GAH_00662
MAC: MA_1491(bioB)
MBAR: MSBR2_0843
MBAK: MSBR3_1290
MMA: MM_2494
MMAC: MSMAC_2246
METM: MSMTP_0959
MTHE: MSTHC_0519
MTHR: MSTHT_0212
MHOR: MSHOH_2818
MFZ: AOB57_001365(cofG)
MBU: Mbur_1302(cofG)
MMET: MCMEM_1749
MMH: Mmah_1608
MPY: Mpsy_2559(cofG)
MTP: Mthe_0221
MCJ: MCON_1058(cofG)
MHI: Mhar_0954
MHU: Mhun_1253
MLA: Mlab_1590
MBG: BN140_0551(cofG)
MEMA: MMAB1_0691(cofG)
MPI: Mpet_0714
MBN: Mboo_2201
MPL: Mpal_0976
MPD: MCP_2458(cofG)
MEZ: Mtc_0996(cofG)
RCI: RCIX461(cofG)
HAL: VNG_1937C
HSL: OE_3722F(cofG)
HHB: Hhub_3263(cofG)
HJE: HacjB3_04935(cofG)
HALH: HTSR_1969(cofG)
HHSR: HSR6_2044(cofG)
HMA: rrnAC3279
HHI: HAH_0520(cofG)
NPH: NP_2398A(cofG)
NMO: Nmlp_2441(cofG)
HUT: Huta_1304
HTI: HTIA_1196
HMU: Hmuk_1415
HALZ: E5139_10230(cofG)
HWA: HQ_3125A(cofG)
HWC: Hqrw_3666(cofG)
HVO: HVO_2201(cofG)
HME: HFX_2258(thiH)
HAER: DU502_15180(cofG)
HALM: FCF25_02485(cofG)
HLA: Hlac_1894
HALB: EKH57_01600(cofG)
HTU: Htur_0642
NMG: Nmag_2633(cofG)
NAT: NJ7G_3940
SALI: L593_00095(cofG)
NMR: Nmar_0593
NID: NPIRD3C_1665(cofG)
NIN: NADRNF5_1568(cofG)
NCT: NMSP_1083(cofG)
NGA: Ngar_c12340(cofG)
NVN: NVIE_011290(cofG)
NEV: NTE_02503
NFN: NFRAN_0114(cofG)
NCV: NCAV_0879(cofG)
NDV: NDEV_0672(cofG)
 » show all
Taxonomy
Reference
1  [PMID:23072415]
  Authors
Decamps L, Philmus B, Benjdia A, White R, Begley TP, Berteau O
  Title
Biosynthesis of F0, precursor of the F420 cofactor, requires a unique two radical-SAM domain enzyme and tyrosine as substrate.
  Journal
J Am Chem Soc 134:18173-6 (2012)
DOI:10.1021/ja307762b
Reference
2  [PMID:25781338]
  Authors
Philmus B, Decamps L, Berteau O, Begley TP
  Title
Biosynthetic versatility and coordinated action of 5'-deoxyadenosyl radicals in deazaflavin biosynthesis.
  Journal
J Am Chem Soc 137:5406-13 (2015)
DOI:10.1021/ja513287k
Other DBs
ExplorEnz - The Enzyme Database: 4.3.1.32
IUBMB Enzyme Nomenclature: 4.3.1.32
ExPASy - ENZYME nomenclature database: 4.3.1.32
BRENDA, the Enzyme Database: 4.3.1.32

DBGET integrated database retrieval system