KEGG   ENZYME: 4.3.1.32
Entry
EC 4.3.1.32                 Enzyme                                 

Name
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase;
FO synthase;
fbiC (gene name) (ambiguous);
cofG (gene name)
Class
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
Sysname
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil ammonia-lyase (7,8-didemethyl-8-hydroxy-5-deazariboflavin-forming)
Reaction(IUBMB)
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + NH3 + L-methionine + 5'-deoxyadenosine [RN:R12162]
Reaction(KEGG)
R12162
Substrate
5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil [CPD:C21971];
S-adenosyl-L-methionine [CPD:C00019]
Product
7,8-didemethyl-8-hydroxy-5-deazariboflavin [CPD:C19154];
NH3 [CPD:C00014];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198]
Comment
The enzyme produces the 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) precursor of the redox cofactor coenzyme F420, which is found in methanogens and in various actinobacteria. FO is also produced by some cyanobacteria and eukaryotes. The enzyme, which forms a complex with EC 2.5.1.147, 5-amino-6-(D-ribitylamino)uracil---L-tyrosine 4-hydroxyphenyl transferase, is a radical SAM enzyme that uses the 5'-deoxyadenosyl radical to catalyse the condensation reaction.
History
EC 4.3.1.32 created 2010 as EC 2.5.1.77, part transferred 2018 to EC 4.3.1.32
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K11779  FO synthase
K11780  7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
Genes
CPS: CPS_4048 CPS_4049
ALT: ambt_18390(cofG)
AAL: EP13_00580
AAUS: EP12_00555
ASP: AOR13_3368
ASQ: AVL57_01945
AAW: AVL56_00755
ALE: AV939_00760
ALZ: AV940_00760
MPC: Mar181_0278
MPRI: MP3633_0352(cofG)
EBA: p2A350
HOH: Hoch_0059
MESP: C1M53_21085(cofG)
RPB: RPB_4423
OCA: OCAR_5007
PZU: PHZ_c2536
PSF: PSE_2793(fbiC)
LAQU: R2C4_00580(cofG)
THAS: C6Y53_09645(cofG)
SWI: Swit_0717
BBEV: BBEV_1716(cofG)
BSE: Bsel_1865
MTU: Rv1173(fbiC)
MTV: RVBD_1173
MTC: MT1210
MRA: MRA_1184(fbiC)
MTG: MRGA327_07395(fbiC)
MTUR: CFBS_1252(fbiC)
MTO: MTCTRI2_1205(fbiC)
MTD: UDA_1173(fbiC)
MTN: ERDMAN_1316(fbiC)
MTJ: J112_06345(fbiC)
MTUE: J114_06345(fbiC)
MTX: M943_06155(fbiC)
MTUL: TBHG_01157
MTUT: HKBT1_1250(fbiC)
MTUU: HKBT2_1256(fbiC)
MTQ: HKBS1_1254(fbiC)
MBO: BQ2027_MB1206(fbiC)
MBB: BCG_1236(fbiC)
MBT: JTY_1209(fbiC)
MBM: BCGMEX_1208(fbiC)
MBX: BCGT_1005
MAF: MAF_11920(fbiC)
MMIC: RN08_1318
MCE: MCAN_11841(fbiC)
MCQ: BN44_11312(fbiC)
MCV: BN43_30241(fbiC)
MCX: BN42_21039(fbiC)
MCZ: BN45_30234(fbiC)
MLE: ML1492(fbiC)
MLB: MLBr01492
MPA: MAP_2611c
MAO: MAP4_1207
MAVI: RC58_05980(fbiC)
MAVU: RE97_05975(fbiC)
MAV: MAV_1312
MIT: OCO_12230
MIA: OCU_12190
MID: MIP_01953
MYO: OEM_12380
MCHI: AN480_07165(fbiC)
MMAL: CKJ54_05940(fbiC)
MLP: MLM_1243
MUL: MUL_1020(fbiC)
MMC: Mmcs_4032
MKM: Mkms_4107
MJL: Mjls_4262
MMI: MMAR_4279(fbiC)
MMAE: MMARE11_40940(fbiC)
MMM: W7S_05970(fbiC)
MLI: MULP_04464(fbiC)
MKN: MKAN_07265(fbiC)
MYV: G155_22255(fbiC)
MYE: AB431_23285(fbiC)
MHAD: B586_07415(fbiC)
MYN: MyAD_20555(fbiC)
MDX: BTO20_08855(fbiC)
MSHG: MSG_03833(fbiC)
MFJ: MFLOJ_48330(fbiC)
MXE: MYXE_14930(fbiC)
MNV: MNVI_05210(fbiC)
MSM: MSMEG_5126(fbiC)
MSG: MSMEI_4998(fbiC)
MSB: LJ00_25350(fbiC)
MSN: LI99_25355(fbiC)
MSH: LI98_25360(fbiC)
MVA: Mvan_4533
MGI: Mflv_2167
MNE: D174_20945(fbiC)
MGO: AFA91_14910(fbiC)
MPHL: MPHLCCUG_04074(mqnE)
MVQ: MYVA_4308(fbiC)
MLL: B1R94_06840(fbiC)
MHAS: MHAS_02879(fbiC)
MDU: MDUV_01960(fbiC)
MCHT: MCHIJ_26390(fbiC)
MDR: MDOR_28370(fbiC)
MAB: MAB_1319
MMV: MYCMA_0695(fbiC)
MABB: MASS_1319(fbiC)
MIZ: BAB75_07105(fbiC)
MSTE: MSTE_01287
MSAO: MYCSP_05810(fbiC)
MSAL: DSM43276_01185(mqnE)
MJD: JDM601_1161(fbiC)
MTER: 4434518_01112(fbiC)
MMIN: MMIN_27930(fbiC)
MHIB: MHIB_06830(fbiC)
MKR: MKOR_00500(fbiC)
NFA: NFA_47520
NCY: NOCYR_4499(fbiC)
NBR: O3I_035915(fbiC)
NNO: NONO_c64550(fbiC)
NSL: BOX37_25530(fbiC)
NSR: NS506_01897(fbiC)
NTP: CRH09_33525(fbiC)
NOZ: DMB37_14595(fbiC)
RER: RER_41830(fbiC)
REY: O5Y_19555(fbiC)
REB: XU06_19700(fbiC)
ROP: ROP_60060(fbiC)
REQ: REQ_14170
RPY: Y013_22830(fbiC)
RHB: NY08_5017
RAV: AAT18_08315(fbiC)
RFA: A3L23_03076(fbiC)
RHW: BFN03_02640(fbiC)
RHS: A3Q41_00251(fbiC)
RRZ: CS378_23345(fbiC)
RHU: A3Q40_02330(fbiC)
RQI: C1M55_20650(fbiC)
RHQ: IM25_12240(fbiC)
RHOD: AOT96_01090(fbiC)
RBY: CEJ39_09030(fbiC)
GIT: C6V83_14745(fbiC)
SCO: SCO4429(fbiC)
SALB: XNR_2222
SMA: SAVERM_3794(cofG)
SGR: SGR_4235
SGB: WQO_14275(fbiC)
SCB: SCAB_52181(fbiC)
SCT: SCAT_2338(fbiC)
SFA: Sfla_3633
SBH: SBI_06333(fbiC)
SHY: SHJG_4710
SVE: SVEN_3084
SDV: BN159_5026(fbiC)
SALS: SLNWT_4334
STRP: F750_3084
SFI: SFUL_2886
SCI: B446_16865(fbiC)
SRC: M271_20760(fbiC) M271_38995(fbiC)
SALU: DC74_3703
SALL: SAZ_19675(fbiC)
SLV: SLIV_16290(fbiC)
SGU: SGLAU_14900(fbiC)
SVT: SVTN_16080(fbiC)
STRE: GZL_05186
SCW: TU94_14370(fbiC)
SLD: T261_4807
SLC: SL103_02830(fbiC)
STRM: M444_15375(fbiC)
STRC: AA958_12800(fbiC)
SAMB: SAM23877_3304(fbiC)
SPRI: SPRI_4317
SCZ: ABE83_21500(fbiC)
SCX: AS200_26105(fbiC)
SRW: TUE45_04323(mqnE_1)
STRF: ASR50_19115(fbiC)
SLE: sle_40200(sle_40200)
SRN: A4G23_02305(mqnE_1)
SPAV: Spa2297_14305(fbiC)
STRT: A8713_13345(fbiC)
SCLF: BB341_11230(fbiC)
SGS: AVL59_44175(fbiC)
STSI: A4E84_17315(fbiC)
SNR: SNOUR_23810(fbiC)
SPLU: LK06_018940(fbiC)
STRD: NI25_22395(fbiC)
SNW: BBN63_19680(fbiC)
SAUO: BV401_26765(fbiC) BV401_35045(fbiC)
SSIA: A7J05_20325(fbiC)
SVU: B1H20_13635(fbiC)
SPUN: BFF78_24080(fbiC)
SGV: B1H19_17935(fbiC)
SLAU: SLA_3135
SALF: SMD44_03660(fbiC)
SALJ: SMD11_3959(fbiC)
SLX: SLAV_16495(mqnE2)
STRO: STRMOE7_16995(fbiC)
SFK: KY5_3501c
SNZ: DC008_14780(fbiC)
SGE: DWG14_04813(fbiC)
SRJ: SRO_4209
SLK: SLUN_16960(fbiC)
SDX: C4B68_22925(fbiC)
SAST: CD934_19070(fbiC)
KSK: KSE_45370(fbiC)
KAB: B7C62_13575(fbiC)
KAU: B6264_08870(fbiC)
NDK: I601_0314(mqnE)
NBE: Back2_26860(fbiC)
KFL: Kfla_6490
PSIM: KR76_06880
TFU: Tfu_2519
NDA: Ndas_3771
NAL: B005_0949(cofG)
NGV: CDO52_24260(fbiC)
STRR: EKD16_20105(mqnE)
TCU: Tcur_4075
SRO: Sros_1376
NOA: BKM31_38790(fbiC)
FAL: FRAAL1263
ACE: Acel_0769
GOB: Gobs_1483
BSD: BLASA_1295(fbiC)
MMAR: MODMU_1466(fbiC)
SEN: SACE_6594
AMD: AMED_6904(fbiC)
AMN: RAM_35420(fbiC)
AMM: AMES_6798(fbiC)
AMZ: B737_6798(fbiC)
AOI: AORI_1815(fbiC)
AJA: AJAP_30405(fbiC)
AMQ: AMETH_5642(fbiC)
AMYC: CU254_27985(fbiC)
AMYB: BKN51_01275(fbiC)
AORI: SD37_32150(fbiC)
ACTI: UA75_18405
ACAD: UA74_17915
AHG: AHOG_15900(mqnE)
ACTA: C1701_18075(fbiC)
ALO: CRK57346
SAQ: Sare_0888
MIL: ML5_1202
MTUA: CSH63_19720(fbiC)
VMA: VAB18032_09475(fbiC)
PLAB: C6361_20395(fbiC)
PLAT: C6W10_23365(fbiC)
CAI: Caci_8766
TBI: Tbis_0746
AEY: CDG81_19075(fbiC)
RXY: Rxyl_0975
SYN: sll1285
SYZ: MYO_16490
SYY: SYNGTS_0642(cofG)
SYT: SYNGTI_0642(cofG)
SYS: SYNPCCN_0642(cofG)
SYQ: SYNPCCP_0642(cofG)
SYC: syc0648_d
SYNC: CB0101_10105(cofG)
TEL: tll1515(cofG)
THN: NK55_04200(cofG)
THEC: FFX45_07980(cofG)
CYI: CBM981_1919(cofG)
HHG: XM38_019080(cofG)
THEU: HPC62_10945(cofG)
MAR: MAE_05370
MPK: VL20_1284
CYL: AA637_08445(cofG)
ENN: FRE64_05620(cofG)
CYT: cce_2717
TER: Tery_0173
ARP: NIES39_K03090(cofG)
PAGH: NIES204_39250(cofG)
OXY: HCG48_04405(cofG)
LFS: HFV01_01570(cofG)
GVI: gll2100(cofG)
GLJ: GKIL_0029(cofG)
ANA: alr3573
NSH: GXM_04355
AVA: Ava_1598
NAZ: Aazo_0829
ANB: ANA_C12049(thiH)
ANN: EH233_29075(cofG)
CALH: IJ00_16040
NSP: BMF81_01795(cofG)
DOU: BMF77_03633(fbiC_2)
DFS: HGD76_23210(cofG)
CCUR: IAR63_10960(cofG)
CTHE: Chro_4336
TRO: trd_A0899(cofH) trd_A0900(cofG)
MJA: MJ_0446
MMP: MMP0876(cofG)
MMD: GYY_05090(cofG)
MMAK: MMKA1_09710(cofG)
MMAO: MMOS7_09500(cofG)
MMAD: MMJJ_01170
MAE: Maeo_0911
MVO: Mvol_0642
MTH: MTH_1198
MMG: MTBMA_c15760(cofG)
METC: MTCT_1091
MWO: MWSIV6_1591(cofG)
METE: tca_01155(fbiC_2)
METK: FVF72_05630(cofG)
MTHM: FZP57_07625(cofG)
MST: Msp_0296(cofG)
METB: AW729_05670(cofG)
MRU: mru_1974(cofG)
MSI: Msm_1035
MEB: Abm4_1547(cofG)
MMIL: sm9_2047(cofG)
MEYE: TL18_09420(cofG)
MOL: YLM1_1412
METH: MBMB1_1721(cofG)
MFC: BRM9_0974(cofG)
MFI: DSM1535_0944(cofG)
MCUB: MCBB_2020(cofG)
METO: CIT02_09285(cofG)
MFV: Mfer_0395
MKA: MK0898(thiH_2)
AFU: AF_0797
FPL: Ferp_0002
GAC: GACE_2058
GAH: GAH_00662
MBAR: MSBR2_0843
MBAK: MSBR3_1290
MAC: MA_1491(bioB)
MMA: MM_2494
MMAC: MSMAC_2246
METM: MSMTP_0959
MTHR: MSTHT_0212
MTHE: MSTHC_0519
MHOR: MSHOH_2818
MFZ: AOB57_001365(cofG)
MBU: Mbur_1302(cofG)
MMET: MCMEM_1749
MMH: Mmah_1608
MPY: Mpsy_2559(cofG)
MZI: HWN40_09690(cofG)
MTP: Mthe_0221
MCJ: MCON_1058(cofG)
MHI: Mhar_0954
MHU: Mhun_1253
MLA: Mlab_1590
MBG: BN140_0551(cofG)
MEMA: MMAB1_0691(cofG)
MPI: Mpet_0714
MBN: Mboo_2201
MPL: Mpal_0976
MPD: MCP_2458(cofG)
MEZ: Mtc_0996(cofG)
RCI: RCIX461(cofG)
HAL: VNG_1937C
HSL: OE_3722F(cofG)
HHB: Hhub_3263(cofG)
HJE: HacjB3_04935(cofG)
HALH: HTSR_1969(cofG)
HHSR: HSR6_2044(cofG)
SALR: FQU85_06450(cofG)
HALR: EFA46_002120(cofG)
HMA: rrnAC3279
HHI: HAH_0520(cofG)
HALJ: G9465_17520(cofG)
NPH: NP_2398A(cofG)
NMO: Nmlp_2441(cofG)
HUT: Huta_1304
HTI: HTIA_1196
HALA: Hrd1104_08990(cofG)
HMU: Hmuk_1415
HALZ: E5139_10230(cofG)
HALL: LC1Hm_0632(cofG)
HRR: HZS55_07080(cofG)
HPEL: HZS54_07640(cofG)
HWA: HQ_3125A(cofG)
HWC: Hqrw_3666(cofG)
HVO: HVO_2201(cofG)
HME: HFX_2258(thiH)
HALE: G3A49_14445(cofG)
HAER: DU502_15180(cofG)
HRA: EI982_15055(cofG)
HALM: FCF25_02485(cofG)
HLA: Hlac_1894
HALB: EKH57_01600(cofG)
HEZZ: EO776_09575(cofG)
HALQ: Hrr1229_010315(cofG)
HALN: B4589_007615(cofG)
HALG: HUG10_08915(cofG)
HALU: HUG12_07185(cofG)
HDF: AArcSl_2518(cofG)
HTU: Htur_0642
HALY: HYG82_19875(cofG)
NMG: Nmag_2633(cofG)
NAT: NJ7G_3940
NVR: FEJ81_14715(cofG)
NPL: FGF80_13915(cofG)
SALI: L593_00095(cofG)
NAS: GCU68_09400(cofG)
NMR: Nmar_0593
NID: NPIRD3C_1665(cofG)
NIN: NADRNF5_1568(cofG)
NIW: Nisw_01895(cofG)
NCT: NMSP_1083(cofG)
NGA: Ngar_c12340(cofG)
NVN: NVIE_011290(cofG)
NEV: NTE_02503
NFN: NFRAN_0114(cofG)
NCV: NCAV_0879(cofG)
NDV: NDEV_0672(cofG)
CCAI: NAS2_0577
 » show all
Reference
1  [PMID:23072415]
  Authors
Decamps L, Philmus B, Benjdia A, White R, Begley TP, Berteau O
  Title
Biosynthesis of F0, precursor of the F420 cofactor, requires a unique two radical-SAM domain enzyme and tyrosine as substrate.
  Journal
J Am Chem Soc 134:18173-6 (2012)
DOI:10.1021/ja307762b
Reference
2  [PMID:25781338]
  Authors
Philmus B, Decamps L, Berteau O, Begley TP
  Title
Biosynthetic versatility and coordinated action of 5'-deoxyadenosyl radicals in deazaflavin biosynthesis.
  Journal
J Am Chem Soc 137:5406-13 (2015)
DOI:10.1021/ja513287k
Other DBs
ExplorEnz - The Enzyme Database: 4.3.1.32
IUBMB Enzyme Nomenclature: 4.3.1.32
ExPASy - ENZYME nomenclature database: 4.3.1.32
BRENDA, the Enzyme Database: 4.3.1.32

DBGET integrated database retrieval system