KEGG   ENZYME: 4.3.1.9Help
Entry
EC 4.3.1.9                  Enzyme                                 

Name
glucosaminate ammonia-lyase;
glucosaminic dehydrase;
D-glucosaminate dehydratase;
D-glucosaminic acid dehydrase;
aminodeoxygluconate dehydratase;
2-amino-2-deoxy-D-gluconate hydro-lyase (deaminating);
aminodeoxygluconate ammonia-lyase;
2-amino-2-deoxy-D-gluconate ammonia-lyase;
D-glucosaminate ammonia-lyase;
D-glucosaminate ammonia-lyase (isomerizing;
2-dehydro-3-deoxy-D-gluconate-forming)
Class
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
BRITE hierarchy
Sysname
2-amino-2-deoxy-D-gluconate ammonia-lyase (isomerizing; 2-dehydro-3-deoxy-D-gluconate-forming)
Reaction(IUBMB)
2-amino-2-deoxy-D-gluconate = 2-dehydro-3-deoxy-D-gluconate + NH3 (overall reaction) [RN:R01544];
(1a) 2-amino-2-deoxy-D-gluconate = (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate + H2O [RN:R10773];
(1b) (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate = (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate (spontaneous) [RN:R10774];
(1c) (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate + H2O = 2-dehydro-3-deoxy-D-gluconate + NH3 (spontaneous) [RN:R10775]
Reaction(KEGG)
Substrate
2-amino-2-deoxy-D-gluconate [CPD:C03752];
(2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate [CPD:C20844];
(4S,5R)-4,5,6-trihydroxy-2-iminohexanoate [CPD:C20845];
H2O [CPD:C00001]
Product
2-dehydro-3-deoxy-D-gluconate [CPD:C00204];
NH3 [CPD:C00014];
(2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate [CPD:C20844];
H2O [CPD:C00001];
(4S,5R)-4,5,6-trihydroxy-2-iminohexanoate [CPD:C20845]
Comment
Contains pyridoxal phosphate. The enzyme releases an unstable enamine product that tautomerizes to an imine form, which undergoes spontaneous hydrolytic deamination to form the final product.
History
EC 4.3.1.9 created 1972, (EC 4.3.1.21 created 1965 as EC 4.2.1.26, transferred 2002 to EC 4.3.1.21, incorporated 2004) modified 2004
Pathway
ec00030  Pentose phosphate pathway
Orthology
K22345  glucosaminate ammonia-lyase
Genes
PAE: PA0849(trxB2)
PAEV: N297_876(trxB)
PAEI: N296_876(trxB)
PAU: PA14_53290(trxB2)
PAP: PSPA7_4671(trxB1)
PAG: PLES_44691(trxB2)
PAF: PAM18_4191(trxB2)
PNC: NCGM2_1623(trxB2)
PAEB: NCGM1900_1877(trxB2)
PDK: PADK2_21460
PAEP: PA1S_22200
PAEM: U769_21570
PAEL: T223_22835
PAEU: BN889_00876(trxB2)
PAEG: AI22_11740
PAEC: M802_872(trxB)
PAEO: M801_876(trxB)
PMY: Pmen_0978
PMK: MDS_1066
PRE: PCA10_47670(trxB)
PPSE: BN5_3403
PPU: PP_0786(trxB)
PPF: Pput_0809
PPT: PPS_0839
PPB: PPUBIRD1_0830(trxB)
PPI: YSA_06637
PPX: T1E_2100(trxB)
PPUH: B479_04405
PPUT: L483_03945
PPUN: PP4_44970(trxB)
PPUD: DW66_0801
PMON: X969_02440
PMOT: X970_02420
PST: PSPTO_1178(trxB)
PSB: Psyr_1016
PSYR: N018_20605
PSP: PSPPH_1067(trxB)
PAMG: BKM19_007795(trxB)
PAVL: BKM03_06735(trxB)
PFL: PFL_0945(trxB_1)
PPRC: PFLCHA0_c09600(trxB1)
PPRO: PPC_0979
PFS: PFLU_5162
PFE: PSF113_4883(trxB2)
PFC: PflA506_4454(trxB)
PFW: PF1751_v1c46110(trxB)
PFB: VO64_2002
PMAN: OU5_2518(trxB)
PEN: PSEEN0924(trxB)
PPUU: PputUW4_00805(trxB1)
PKC: PKB_4627(trxb3)
PSES: PSCI_0771
PSEM: TO66_04840
PSOS: POS17_0950
PANR: A7J50_4906
PSET: THL1_4770
PSIL: PMA3_24915
 » show all
Taxonomy
Reference
1
  Authors
Imanaga, Y.
  Title
Metabolism of D-glucosamine. III. Enzymic degradation of D-glucosaminic acid.
  Journal
J Biochem (Tokyo) 45:647-650 (1958)
Reference
2
  Authors
Merrick, J.M. and Roseman, S.
  Title
D-Glucosaminic acid dehydrase.
  Journal
Methods Enzymol 9:657-660 (1966)
Reference
3  [PMID:7068563]
  Authors
Iwamoto R, Imanaga Y, Soda K.
  Title
D-glucosaminate dehydratase from Agrobacterium radiobacter. Physicochemical and enzymological properties.
  Journal
J Biochem (Tokyo) 91:283-9 (1982)
Reference
4  [PMID:7766176]
  Authors
Iwamoto R, Taniki H, Koishi J, Nakura S.
  Title
D-glucosaminate aldolase activity of D-glucosaminate dehydratase from Pseudomonas fluorescens and its requirement for Mn2+ ion.
  Journal
Biosci Biotechnol Biochem 59:408-11 (1995)
DOI:10.1271/bbb.59.408
Other DBs
ExplorEnz - The Enzyme Database: 4.3.1.9
IUBMB Enzyme Nomenclature: 4.3.1.9
ExPASy - ENZYME nomenclature database: 4.3.1.9
BRENDA, the Enzyme Database: 4.3.1.9
CAS: 37290-91-8

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