KEGG ENZYME: 4.3.3.2

ENZYME: 4.3.3.2

Entry

EC 4.3.3.2                  Enzyme

Name

strictosidine synthase;
strictosidine synthetase;
STR;
3-alpha(S)-strictosidine tryptamine-lyase

Class

Lyases;
Carbon-nitrogen lyases;
Amine-lyases

Sysname

3-alpha(S)-strictosidine tryptamine-lyase (secologanin-forming)

Reaction(IUBMB)

3-alpha(S)-strictosidine + H2O = tryptamine + secologanin [RN:R03738]

Reaction(KEGG)

R03738

Substrate

3-alpha(S)-strictosidine [CPD:C03470];
H2O [CPD:C00001]

Product

tryptamine [CPD:C00398];
secologanin [CPD:C01852]

Comment

Catalyses a Pictet-Spengler reaction between the aldehyde group of secologanin and the amino group of tryptamine [4,5]. Involved in the biosynthesis of the monoterpenoid indole alkaloids.

History

EC 4.3.3.2 created 1990

Pathway

ec00901

Indole alkaloid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K01757

strictosidine synthase

Genes

ATH:	AT1G74000(SS3) AT1G74010 AT1G74020(SS2)

ALY:	9323583 9325020 9325021

CRB:	111828550 17895532 17896456

CSAT:

104702105 104702108 104713027 104713028 104713029 104713030 104751270 104751271 104751272

EUS:	EUTSA_v10018851mg EUTSA_v10018854mg EUTSA_v10019730mg

BRP:	103830721 103831875 103831876 103852873 103852875 103852877

BNA:	106357248 106357249 106379410 106379413 106379465 106379467 106381362 106403770 106403773 106405344 106411924 106414096 106414626 111212904 125581726 125601147 125601148

BOE:	106299100 106299368 106299590 106299599 106322831 106325777 106326024

RSZ:	108816729 108816754 108816770 108817529 108817537 108824205 108836146 108840604 108840993 108858470

THJ:

104808295

Reference

1 [PMID:510306]

Authors

Treimer JF, Zenk MH.

Title

Purification and properties of strictosidine synthase, the key enzyme in indole alkaloid formation.

Journal

Eur J Biochem 101:225-33 (1979)
DOI:10.1111/j.1432-1033.1979.tb04235.x

Reference

2 [PMID:7763429]

Authors

Kutchan TM.

Title

Strictosidine: from alkaloid to enzyme to gene.

Journal

Phytochemistry 32:493-506 (1993)
DOI:10.1016/S0031-9422(00)95128-8

Reference

3 [PMID:7887913]

Authors

de Waal A, Meijer AH, Verpoorte R.

Title

Strictosidine synthase from Catharanthus roseus: purification and characterization of multiple forms.

Journal

Biochem J 306 ( Pt 2):571-80 (1995)
DOI:10.1042/bj3060571

Reference

4 [PMID:16133216]

Authors

Ruppert M, Woll J, Giritch A, Genady E, Ma X, Stockigt J

Title

Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.

Journal

Planta 222:888-98 (2005)
DOI:10.1007/s00425-005-0031-0

Reference

5 [PMID:16481164]

Authors

McCoy E, Galan MC, O'Connor SE.

Title

Substrate specificity of strictosidine synthase.

Journal

Bioorg Med Chem Lett 16:2475-8 (2006)
DOI:10.1016/j.bmcl.2006.01.098

Reference

6 [PMID:16531499]

Authors

Ma X, Panjikar S, Koepke J, Loris E, Stockigt J.

Title

The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins.

Journal

Plant Cell 18:907-20 (2006)
DOI:10.1105/tpc.105.038018

Sequence

[ag:CAA44208]

Other DBs

ExplorEnz - The Enzyme Database:

4.3.3.2

IUBMB Enzyme Nomenclature:

4.3.3.2

ExPASy - ENZYME nomenclature database:

4.3.3.2

BRENDA, the Enzyme Database:

4.3.3.2

CAS:	69669-72-3

DBGET integrated database retrieval system