Entry
Name
mimosinase;
mimosine amidohydrolase (incorrect)
Class
Lyases;
Carbon-nitrogen lyases;
Amine-lyases
BRITE hierarchy
Sysname
(2S)-2-amino-3-[3-hydroxy-4-oxopyridin-1(4H)-yl]propanoate 3-hydroxy-4H-pyrid-4-one-lyase (2-aminoprop-2-enoate-forming)
Reaction(IUBMB)
L-mimosine + H2O = 3-hydroxy-4H-pyrid-4-one + pyruvate + NH3 (overall reaction) [RN:
R04350 ];
(1a) L-mimosine = 3-hydroxy-4H-pyrid-4-one + 2-aminoprop-2-enoate [RN:
R13116 ];
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous) [RN:
R11099 ];
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous) [RN:
R11100 ]
Reaction(KEGG)
Substrate
Product
Comment
A pyridoxal 5'-phosphate protein. The enzyme degrades the toxic amino acid L-mimosine. It cleaves a carbon-nitrogen bond, releasing 3-hydroxy-4H-pyrid-4-one and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. It is thought to have evolved from EC
4.4.1.13 , cysteine-S-conjugate beta-lyase. It has been described in both mimosine-producing plants and some bacteria.
History
EC 4.3.3.8 created 1989 as EC 3.5.1.61, transferred 2022 to EC 4.3.3.8
Orthology
Genes
PCIN : 129286560 129309431 129309784 129310132
MICE : EHF32_12190 EHF32_15055
» show all
Taxonomy
Reference
1
Authors
Tangendjaja, B., Lowry, J.B. and Wills, R.H.
Title
Isolation of a mimosine degrading enzyme from Leucaena leaf.
Journal
J Sci Food Agric 37:523-526 (1986)
Reference
Authors
Negi VS, Bingham JP, Li QX, Borthakur D.
Title
A carbon-nitrogen lyase from Leucaena leucocephala catalyzes the first step of mimosine degradation.
Journal
Reference
Authors
Oogai S, Fukuta M, Watanabe K, Inafuku M, Oku H.
Title
Molecular characterization of mimosinase and cystathionine beta-lyase in the Mimosoideae subfamily member Mimosa pudica.
Journal
Sequence
Reference
Authors
Oogai S, Fukuta M, Inafuku M, Oku H.
Title
Isolation and characterization of mimosine degrading enzyme from Arthrobacter sp. Ryudai-S1.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.3.3.8
ExPASy - ENZYME nomenclature database: 4.3.3.8
BRENDA, the Enzyme Database: 4.3.3.8