KEGG ENZYME: 4.3.99.4

ENZYME: 4.3.99.4

Entry

EC 4.3.99.4                 Enzyme

Name

choline trimethylamine-lyase;
cutC (gene name)

Class

Lyases;
Carbon-nitrogen lyases;
Other carbon-nitrogen lyases

Sysname

choline trimethylamine-lyase (acetaldehyde-forming)

Reaction(IUBMB)

choline = trimethylamine + acetaldehyde [RN:R10285]

Reaction(KEGG)

R10285

Substrate

choline [CPD:C00114]

Product

trimethylamine [CPD:C00565];
acetaldehyde [CPD:C00084]

Comment

The enzyme utilizes a glycine radical to break the C-N bond in choline. Found in choline-degrading anaerobic bacteria.

History

EC 4.3.99.4 created 2013

Orthology

K20038

choline trimethylamine-lyase

Genes

ECP:

ECP_4600

ECT:	ECIAI39_4211

EOC:

CE10_4248

ECI:	UTI89_C4964

ELU:	UM146_22045

EFE:

EFER_1995

ECAN:

CWI88_16610(cutC)

EPT:	HWQ17_06955(cutC)

KPR:

KPR_0821

KPB:	FH42_20250

KVA:

Kvar_4520

KPE:

KPK_4883

KVQ:	SP68_17815

KQU:	AVR78_07575

KOX:

KOX_09770

KOE:

A225_0656

KOY:	J415_27975

KOM:	HR38_08490

KOK:	KONIH1_03490

KOC:	AB185_32690

EAE:

EAE_10070

EAR:

CCG31976

KLL:	BJF97_04030

KLW:	DA718_25030(cutC)

KGR:	JJJ10_24475(cutC)

KPAS:

LUW96_09995(cutC)

KLC:	K7H21_24650(cutC)

KLM:	BWI76_03575

REE:	electrica_04429(bssA_2)

KLP:	GUC22_23705(cutC)

KLX:	GQR96_02855(cutC)

ROR:	RORB6_15750

RON:	TE10_05280

RPLN:

B1209_23720

RAO:	DSD31_22540(cutC)

EBT:	EBL_c07460

KIE:	NCTC12125_01928(csdB_1)

KAS:	KATP_29140(cutC_2)

KLU:	K7B04_14115(cutC)

KCY:	RIN60_14310(cutC)

KGN:	GY169_15070(cutC)

LEI:	C2U54_12990(cutC)

LER:	GNG29_08160(cutC)

LEA:	GNG26_07795(cutC)

LEY:	DVA43_09635(cutC)

YRE:	HEC60_11550(cutC)

IZH:	FEM41_20680(cutC)

EBF:

D782_3573

EBU:	CUC76_12255(cutC)

SMAF:

D781_0477

SFW:	WN53_11110

SFG:	AV650_06355

SRZ:	AXX16_0069

SERM:

CLM71_02375(cutC)

SRHZ:

FO014_07615(cutC)

SERT:

TAN611_0544

ECA:

ECA3768

PATR:

EV46_18475

PATO:

GZ59_37640

PCT:

PC1_3543

PCV:	BCS7_17765

PWA:	Pecwa_3714

PPAR:

A8F97_00095

PEC:

W5S_3855

PWS:	A7983_03620

PBRA:

B5S52_03785

PARI:

I2D83_04105(cutC)

PPEU:

DUT67_03670(cutC)

BGJ:	AWC36_11290

ERWI:

GN242_15600(cutC)

PMR:

PMI2716

PMIB:

BB2000_2716

PVL:	AOB99_14810

PVG:	CRN77_06060(cutC)

PHAU:

PH4a_05120(cutC)

PROT:

BTA34_14845

PCOL:

F1325_17050(cutC)

PCIB:

F9282_17410(cutC)

PPEE:

I6G31_11550(cutC)

PAPP:

QQS39_16690(cutC)

PRON:

LG402_18585(cutC)

PRG:	RB151_038860(bssA)

PALA:

CO695_06855(cutC)

PHEI:

NCTC12003_03419(csdB)

PRQ:	CYG50_19285(cutC)

PRJ:	NCTC6933_03477(csdB)

PHAG:

PZ638_17665(cutC)

PZJ:	QS795_015250(cutC)

PXH:	ACE2AL_17560(cutC)

PLAZ:

ACIH2S_16980(cutC)

MWI:	MNY66_01680(cutC)

PFQ:	QQ39_10975

VFU:	vfu_A00327

VIC:	SNR28_02490(cutC) SNR28_22375(cutC)

PGH:	FH974_04435(cutC)

PATE:

NNL38_10090(cutC)

MYA:	MORIYA_2257(cutC)

AHA:

AHA_1330

AHY:	AHML_07230

AHD:	AI20_12520

AHR:	V428_07450

AHP:	V429_07455

AHJ:	V469_16045

AHI:	VU14_16045

AAJ:	BOQ57_06470

AALL:

I6G90_11510(cutC)

AEJ:	E5E97_11305(cutC)

ABES:

IU367_15325(cutC)

AERO:

CK910_21615(cutC)

AERZ:

E5E96_14155(cutC)

AOJ:	ACPDZI_06430(cutC)

PCA:

Pcar_0937

PACE:

A6070_03930

DDS:

Ddes_1357

DFI:	AXF13_01055

DEF:	CNY67_08905(cutC)

PSYF:

N1030_14315(cutC)

DSB:	LN040_10155(cutC)

DDE:	Dde_3039 Dde_3282

DSA:	Desal_0468

DHY:	DESAM_21354

PSEL:

GM415_17810(cutC)

PBIZ:

LWC08_01655(cutC) LWC08_03585(cutC)

TMUR:

JBF11_09630

BWS:	WCP94_000823 WCP94_000825

DPS:

DP1823

DEO:	CAY53_01550

DOV:	DSCO28_56630(pflD_1)

DALK:

DSCA_27500(pflD)

DEK:	DSLASN_11590(cutC) DSLASN_11760

PTHI:

NDS46_14855(cutC)

PDY:	QJQ58_07110(cutC)

SDG:	SDE12394_10005

SDA:	GGS_1829(pflD)

SDQ:	SDSE167_2084

SIE:

SCIM_0634

SIK:

K710_0229

SIQ:	DQ08_01100

SIO:	DW64_01095

SIZ:	SI82_01350

SRQ:	SR187_4385

SMEN:

SAMEA4412692_2069(csdB)

ESS:	ATZ33_06450

EGV:	EGCR1_04740(cutC) EGCR1_04815(cutC)

EAV:	EH197_03025(cutC)

ERAF:

J9537_16430(cutC)

EDS:	PML78_10185(cutC)

ERX:	ATZ35_14985

VPI:	BW732_05620

VLC:	G314FT_01760(cutC_1)

CTC:

CTC_01449

CTET:

BN906_01604

CBO:

CBO2118

CBA:

CLB_2055

CBH:

CLC_2060

CBY:

CLM_2326

CBL:

CLK_1571

CBK:

CLL_A1453

CBB:

CLD_2523

CBI:

CLJ_B2322

CBN:

CbC4_1734

CBT:

CLH_1376

CBF:

CLI_2161

CBM:

CBF_2145

CBJ:	H04402_02123

CKL:

CKL_3002

CKR:

CKR_2652

CLJ:	CLJU_c39830(pflB3)

CAH:	CAETHG_1829(cutC)

CBV:

U729_818

CSQ:	CSCA_1998 CSCA_4483

CLD:	CLSPO_c21310(pflD1)

CACE:

CACET_c30180(pflD3)

CCK:	Ccar_04680 Ccar_19035

CFM:	BJL90_00710

CCHV:

BTM20_11075

CARG:

RSJ17_18930

CDRK:

B9W14_09555 B9W14_22580

CSEP:

CP523_12430(cutC)

CFER:

D4Z93_03610 D4Z93_07710(cutC)

CLOS:

DMR38_19860(cutC)

CTAG:

LL095_06640(cutC) LL095_08905(cutC)

CLOD:

C820_001268(cutC)

CLOO:

RCG18_19985 RCG18_22805(cutC)

CCAK:

ACFLKC_09345(cutC)

HHW:	NCTC503_01836(csdB_1)

CRS:	FQB35_03225(cutC)

CRAS:

KVH43_06725(cutC)

RGV:	NQ502_13880 NQ502_13920

CAPR:

EQM14_09350

AGAT:

RWV98_03120 RWV98_17455

DSY:

DSY5006

DHD:

Dhaf_4905

DDH:	Desde_4197

DOR:	Desor_5421

DAI:	Desaci_1172 Desaci_1570

DMI:	Desmer_4389

DRM:

Dred_3278

DRU:	Desru_2090 Desru_2143

DFG:	B0537_00305

FWA:	DCMF_12665

EMT:	CPZ25_013730

ELIM:

B2M23_03595

CPY:

Cphy_1417

LACY:

A4V08_24145

CHYL:

CE91St63_01990(pflD_1) CE91St63_28330(pflD_3)

CSH:	Closa_4018

LXA:	OW255_01725(cutC)

LSIA:

VIG27_16030(cutC)

ARF:	AR1Y2_3056

CASP:

NQ535_08220(cutC)

WHJ:	H9Q79_08745 H9Q79_08800

HHJ:	NQ487_06625(cutC)

CPRO:

CPRO_22300(bssA)

ANV:	RBQ60_04550

AMIJ:

EQM06_03460(cutC)

AMIC:

Ami3637_03445(cutC) Ami3637_06840

ABUT:

Ami103574_05650(cutC)

AHAD:

FRZ06_12505(cutC)

AMT:	Amet_1986 Amet_3836

AOE:	Clos_2398 Clos_2688

GFE:	Gferi_19275

CDF:	CD630_11200(pflD)

PDC:	CDIF630_01264

CDC:	CD196_0973(dhaB1)

CDL:	CDR20291_0951(dhaB1)

PDF:	CD630DERM_11200(pflD)

TMY:	TEMA_12490(cutC_1) TEMA_21140(cutC_2)

TPET:

TPHSE_32040(cutC_2)

TVT:	TVTCOM_30920(cutC_1)

TEB:	T8CH_2113(cutC)

THYR:

P4S50_00990 P4S50_06655(cutC)

IBR:	NQ514_09805

EAC:	EAL2_808p07030(pflD)

AVG:	I6H45_07905(cutC)

AOB:	I6H46_01860(cutC)

TIZ:	RBU61_00405

SEDZ:

JYG23_13220(cutC)

SEDM:

RBQ61_04115

MHW:	ACT01_04810

MEG:	DKB62_05650

PFT:

JBW_03217

STED:

SPTER_31890(cutC_3) SPTER_38880(cutC_5)

SSID:

SPSIL_048600(cutC_2)

SACV:

SPACI_007150(cutC_1)

AIN:	Acin_2025(pflD)

OLS:

Olsu_0306

TIO:	INP52_02315(cutC)

CPOR:

BED41_04350 BED41_04450 BED41_11825

AMIZ:

RBH88_08265

» show all

Reference

1 [PMID:23151509]

Authors

Craciun S, Balskus EP

Title

Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme.

Journal

Proc Natl Acad Sci U S A 109:21307-12 (2012)
DOI:10.1073/pnas.1215689109

Sequence

[dde:Dde_3282] [dds:Ddes_1357]

Other DBs

ExplorEnz - The Enzyme Database:

4.3.99.4

IUBMB Enzyme Nomenclature:

4.3.99.4

ExPASy - ENZYME nomenclature database:

4.3.99.4

BRENDA, the Enzyme Database:

4.3.99.4

All links

Gene (1283)   
   MGENES (1022)   
   RefGene (261)   
Protein sequence (3658)   
   UniProt (540)   
   SWISS-PROT (1)   
   RefSeq(pep) (3095)   
   PDBSTR (22)   
DNA sequence (23812)   
   RefSeq(nuc) (19181)   
   GenBank (2335)   
   EMBL (2296)   
3D Structure (7)   
   PDB (7)   
All databases (28760)   

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