KEGG ENZYME: 4.3.99.5

ENZYME: 4.3.99.5

Entry

EC 4.3.99.5                 Enzyme

Name

nitrosuccinate lyase;
creD (gene name)

Class

Lyases;
Carbon-nitrogen lyases;
Other carbon-nitrogen lyases

Sysname

2-nitrobutanedioate lyase (fumarate-forming)

Reaction(IUBMB)

2-nitrobutanedioate = fumarate + nitrite [RN:R12947]

Reaction(KEGG)

R12947

Substrate

2-nitrobutanedioate [CPD:C22388]

Product

fumarate [CPD:C00122];
nitrite [CPD:C00088]

Comment

The enzyme, found in some Actinobacteria, is involved in a pathway that forms nitrite, which is subsequently used to generate a diazo group in some secondary metabolites.

History

EC 4.3.99.5 created 2021

Pathway

ec00998

Biosynthesis of various antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K25597

nitrosuccinate lyase

Genes

MYE:	AB431_04530

MDX:	BTO20_27375

MRH:	MycrhN_2393

MCRO:

MI149_04805

MAB:

MAB_0290

MMV:	MYCMA_0155

MABB:

MASS_0292

MABL:

MMASJCM_0289

MCHE:

BB28_01435

MIZ:	BAB75_01985

MSAO:

MYCSP_01265

MSAL:

DSM43276_00246(pcaB)

NTP:	CRH09_09110

NVU:	V7968_41010

SFI:

SFUL_3321

SANL:

KZO11_09810(pcaB)

SGRF:

SGFS_087140

SCB:	SCAB_69771

SMAL:

SMALA_1626

SSOI:

I1A49_11270(pcaB)

SBH:

SBI_03174

SDV:	BN159_4421 BN159_7958(pcaB3)

SALS:

SLNWT_1070

SGU:	SGLAU_17370

STRE:

GZL_07532

STRM:

M444_36510

STRC:

AA958_10045

SAMB:

SAM23877_0157(pcaB)

SCZ:	ABE83_19260

SCX:	AS200_01105

SPAV:

Spa2297_26590

SGS:	AVL59_39890

SGM:	GCM10017557_24330

SCAD:

DN051_33880

SNR:	SNOUR_34630

SPLU:

LK06_026290 LK06_027150 LK06_029035

SNW:	BBN63_20145

SAUO:

BV401_11595

SALJ:

SMD11_3244(pcaB)

SFK:

KY5_6723

SQZ:	FQU76_00320(pcaB) FQU76_33420(pcaB)

SKA:	CP970_07030

SGZ:	C0216_00545(pcaB)

SVN:	CP980_14845(pcaB)

SRK:	FGW37_24840(pcaB)

SSPO:

DDQ41_19485(pcaB)

SPAD:

DVK44_34380(pcaB)

SCAV:

CVT27_15275(pcaB)

SSEO:

D0Z67_01810

SBY:	H7H31_09705(pcaB) H7H31_25345(pcaB)

SSPB:

CP982_28260(pcaB)

SHUN:

DWB77_00269(pcaB)

SFEU:

IM697_30745

SMOB:

J7W19_14370

SROI:

IAG44_34515(pcaB)

STRY:

EQG64_16060(pcaB)

SLF:	JEQ17_24410

SDEC:

L3078_11360

SAKB:

K1J60_34180

SCIR:

STRCI_003010(pcaB) STRCI_004223(pcaB)

SXT:	KPP03845_106228(pcaB) KPP03845_300045(pcaB)

SGK:	PET44_33950(pcaB)

SYUN:

MOV08_34915(pcaB)

STRZ:

OYE22_09340(pcaB) OYE22_24640(pcaB)

SPRF:

K4G22_13865

SCAJ:

O1G22_43460(pcaB)

STII:

DRB96_16115(pcaB)

STIC:

DRB89_06140(pcaB)

SPAZ:

ABXI76_06575(pcaB)

SPOF:

P8A21_16215

SCT:	SCAT_5480(pcaB)

SCY:	SCATT_54780

KSK:

KSE_07790

STRI:

C7M71_014695(pcaB)

STRH:

GXP74_01845

ABRY:

NYE86_02790(pcaB)

AREV:

RVR_4139(fur17)

AWW:	G8758_12055

MYU:	M8233_09630

MLY:	CJ228_008140

MPOR:

KW076_03915

PALU:

CJ193_005665

PROU:

AB1046_05110

NCG:	KGD84_13305

AGRA:

AGRA3207_000016(pcaB)

AMAZ:

LUW76_01405(pcaB)

ACIR:

OG979_40115(pcaB)

SPIQ:

OHA34_05290(pcaB)

MHAI:

OHB01_07995(pcaB) OHB01_28505(pcaB)

SACG:

FDZ84_06755

ARHD:

VSH64_39005(pcaB)

AMOG:

QRX60_22460

ACOR:

LCL61_30760

AMYZ:

HUW46_08664(pcaB)

ACAB:

QRX50_06060(pcaB)

ANW:	QP939_11875(pcaB)

AMYD:

K1T34_20080(pcaB)

ABAR:

AMYBAR_000889

AMYE:

MJQ72_02265(pcaB)

APRE:

CNX65_23225(pcaB)

AMI:

Amir_4654

SESP:

BN6_36100

SSYI:

EKG83_10130(pcaB) EKG83_27600

KAL:

KALB_2260

KUT:	JJ691_50900(pcaB)

KBR:	Q4V65_12640

KPHY:

AOZ06_18045 AOZ06_45895

UME:	RM788_05835(pcaB) RM788_07365(pcaB)

MICB:

MicB006_2941

MICH:

FJK98_14150(pcaB)

MENO:

Jiend_53580

MCHL:

PVK74_09660

PLK:	CIK06_12450

DFU:	Dfulv_23920(pcaB)

» show all

Reference

1 [PMID:26689788]

Authors

Sugai Y, Katsuyama Y, Ohnishi Y.

Title

A nitrous acid biosynthetic pathway for diazo group formation in bacteria.

Journal

Nat Chem Biol 12:73-5 (2016)
DOI:10.1038/nchembio.1991

Sequence

[ag:BAU09301]

Reference

2 [PMID:30120394]

Authors

Hagihara R, Katsuyama Y, Sugai Y, Onaka H, Ohnishi Y.

Title

Novel desferrioxamine derivatives synthesized using the secondary metabolism-specific nitrous acid biosynthetic pathway in Streptomyces davawensis.

Journal

J Antibiot (Tokyo) 71:911-919 (2018)
DOI:10.1038/s41429-018-0088-1

Sequence

[sdv:BN159_4421]

Other DBs

ExplorEnz - The Enzyme Database:

4.3.99.5

IUBMB Enzyme Nomenclature:

4.3.99.5

ExPASy - ENZYME nomenclature database:

4.3.99.5

BRENDA, the Enzyme Database:

4.3.99.5

All links

Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (6)   
   InterPro (6)   
All databases (10)   

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