KEGG   ENZYME: 4.4.1.13Help
Entry
EC 4.4.1.13                 Enzyme                                 

Name
cysteine-S-conjugate beta-lyase;
cysteine conjugate beta-lyase;
glutamine transaminase K/cysteine conjugate beta-lyase;
L-cysteine-S-conjugate thiol-lyase (deaminating);
cystathionine beta-lyase;
beta-cystathionase;
cystine lyase;
cystathionine L-homocysteine-lyase (deaminating);
L-cystathionine L-homocysteine-lyase (deaminating);
CBL;
S-alkylcysteine lyase;
S-alkylcysteinase;
alkylcysteine lyase;
S-alkyl-L-cysteine sulfoxide lyase;
S-alkyl-L-cysteine lyase;
S-alkyl-L-cysteinase;
alkyl cysteine lyase;
S-alkyl-L-cysteine alkylthiol-lyase (deaminating)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
L-cysteine-S-conjugate thiol-lyase (deaminating; 2-aminoprop-2-enoate-forming)
Reaction(IUBMB)
an L-cysteine-S-conjugate + H2O = a thiol + NH3 + pyruvate (overall reaction) [RN:R03528];
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoprop-2-enoate;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
R03528;
(other) R09366
Show
Substrate
L-cysteine-S-conjugate [CPD:C02882];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
thiol [CPD:C01336]
Comment
A pyridoxal-phosphate protein. The enzyme is promiscuous regarding the moiety conjugated to L-cysteine, and can accept both aliphatic and aromatic substitutions. The enzyme cleaves a carbon-sulfur bond, releasing a thiol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. While bacteria and plants have dedicated enzymes, all of the animal enzymes discovered thus far are bifunctional, most of which also act as aminotransferases.
History
EC 4.4.1.13 created 1981, modified 2018 (EC 4.4.1.6 created 1965, deleted 1972, reinstated 1976, incorporated 2018) (EC 4.4.1.8 created 1972, incorporated 2018)
Pathway
ec00450  Selenocompound metabolism
Orthology
K00816  kynurenine---oxoglutarate transaminase / cysteine-S-conjugate beta-lyase / glutamine---phenylpyruvate transaminase
Genes
HSA: 56267(KYAT3) 883(KYAT1)
PTR: 456999(KYAT3) 473069(KYAT1)
PPS: 100980672(KYAT1) 100993985(KYAT3)
GGO: 101128027(KYAT3) 101137144(KYAT1)
PON: 100436953(KYAT3) 100452866(KYAT1)
NLE: 100579800(KYAT3) 100598335(KYAT1)
MCC: 694023(KYAT3) 717142(KYAT1)
MCF: 101925086(KYAT3) 102142392(KYAT1)
CSAB: 103224481(KYAT3) 103239781(KYAT1)
RRO: 104660517(KYAT1) 104664603(KYAT3)
RBB: 108512836(KYAT1) 108514569(KYAT3)
CJC: 100390682(KYAT1) 100392205(KYAT3)
SBQ: 101034905(CCBL1) 101049363(CCBL2)
MMU: 229905(Kyat3) 70266(Kyat1)
RNO: 311844(Kyat1) 541589(Kyat3)
CGE: 100769821(Kyat1) 100771070(Kyat3)
NGI: 103730106(Kyat3) 103742867(Kyat1)
HGL: 101701445(Kyat3) 101714047(Ccbl1)
CCAN: 109682136(Kyat3) 109697703(Kyat1)
OCU: 100351221(KYAT1) 100358799(KYAT3)
TUP: 102484200(KYAT3) 102492301(KYAT1)
CFA: 479959(KYAT3) 491310(KYAT1)
AML: 100466080(KYAT3) 100484335(KYAT1)
UMR: 103662922(KYAT3) 103670433(KYAT1)
ORO: 101379355(CCBL2) 101381784(CCBL1)
FCA: 101082171(KYAT3) 101082605(KYAT1)
PTG: 102956075(KYAT1) 102960707(KYAT3)
AJU: 106966305(KYAT1) 106968046(KYAT3)
BTA: 104968656 508712(KYAT3) 528582(CCBL1)
BOM: 102268017(KYAT3) 102274277(KYAT1) 102274974
BIU: 109556093(KYAT3) 109563538 109566527(KYAT1)
PHD: 102319020(KYAT3) 102328319(KYAT1)
CHX: 102180605(KYAT1) 102182368(KYAT3)
OAS: 101117485(CCBL1) 101119265(CCBL2)
SSC: 100157176(KYAT3) 100515071(KYAT1)
CFR: 102514713(KYAT1) 102522240(KYAT3)
CDK: 105084526(KYAT3) 105092558(KYAT1)
BACU: 103001193(KYAT3) 103010164(KYAT1)
LVE: 103073927(KYAT1) 103090268(KYAT3)
OOR: 101285560(CCBL2) 101290033(CCBL1)
ECB: 100052141(KYAT3) 100070151(KYAT1)
EPZ: 103550870(KYAT1) 103557170(KYAT3)
EAI: 106831581(KYAT3) 106845422(KYAT1)
MYB: 102246710(KYAT1) 102246937(KYAT3)
MYD: 102770113(KYAT1) 102774189(KYAT3)
HAI: 109376722(KYAT3) 109382267(KYAT1)
RSS: 109445910(KYAT1) 109458284(KYAT3)
PALE: 102885606(KYAT3) 102890346(KYAT1)
LAV: 100674418(KYAT1) 100676040(KYAT3)
MDO: 100013642(KYAT1) 100027687(KYAT3)
SHR: 100925132(KYAT1) 100928200(KYAT3)
OAA: 100074153(CCBL1) 100074828(KYAT3)
GGA: 417202(CCBL1) 424517(KYAT3)
MGP: 100545698(KYAT1) 100546759(KYAT3)
CJO: 107317420(KYAT3) 107321728(KYAT1)
APLA: 101796959(KYAT3) 101804188(KYAT1)
ACYG: 106034104(CCBL2) 106047313(CCBL1)
TGU: 100223564(KYAT3) 100226378(KYAT1)
GFR: 102043066(KYAT3) 102045568(KYAT1)
FAB: 101809203(KYAT1) 101816765(KYAT3)
PHI: 102108384(KYAT3) 102109435(KYAT1)
PMAJ: 107207980(KYAT3) 107212063(KYAT1)
CCW: 104688281(KYAT1) 104695321(KYAT3)
FPG: 101923826(KYAT3) 101924602(KYAT1)
FCH: 102052592(KYAT1) 102055107(KYAT3)
CLV: 102092812(KYAT3) 102097970(KYAT1)
EGZ: 104122512(CCBL1) 104122910(CCBL2)
AAM: 106490298(KYAT1) 106499802(KYAT3)
ASN: 102377511(KYAT3) 102381063(KYAT1)
AMJ: 102564187(KYAT1) 102574637(KYAT3)
PSS: 102446036(KYAT1) 102459761(KYAT3)
CMY: 102931085(KYAT1) 102947846(KYAT3)
CPIC: 101948590(KYAT3) 101949140(KYAT1)
ACS: 100555601(kyat3)
PVT: 110079452(KYAT3) 110079688(KYAT1)
PBI: 103054557(KYAT3) 103058068(KYAT1)
GJA: 107115481(KYAT1) 107126241(KYAT3)
XLA: 108715667(kyat3.S) 380432(kyat3.L) 734936(kyat1.L)
XTR: 496736(kyat1)
NPR: 108791027(KYAT3) 108794311(KYAT1)
DRE: 393315(ccbl2) 791592(kyat1)
AMEX: 103027162(kyat3) 103046817(kyat1)
TRU: 101064758(kyat1) 101066451(kyat3)
LCO: 104931917(kyat3) 104939087(kyat1)
NCC: 104946129(ccbl1) 104955761(ccbl2)
MZE: 101466800(kyat1) 101486988(kyat3)
OLA: 101164171(kyat1) 101173380
XMA: 102235261(kyat3) 102237088(kyat1)
PRET: 103463971(kyat3) 103473416(kyat1)
NFU: 107374788(ccbl1) 107397216(ccbl2)
CSEM: 103376809(kyat3) 103390101(kyat1)
LCF: 108874059 108874060(kyat1) 108878962(kyat3)
HCQ: 109517170(kyat1) 109529744(kyat3)
SASA: 100195431(kat3) 106569847
ELS: 105015286(kyat1) 105030533(kyat3)
SFM: 108921915
LCM: 102348380(KYAT1) 102366870(KYAT3)
CMK: 103171755(kyat1) 103174679(kyat3)
CIN: 100186481
SPU: 593717
APLC: 110981090
SKO: 102808740
DSI: Dsimw501_GD20629(Dsim_GD20629)
MDE: 101891461
AAG: 5564787
AME: 724811(GB15834)
BIM: 100744283
BTER: 100644987
SOC: 105199950
AEC: 105149700
ACEP: 105618986
PBAR: 105433236
HST: 105180580
CFO: 105252539
LHU: 105673051
PGC: 109856505
NVI: 100119206
DPA: 109532996
NVL: 108565563
BMOR: 101742893
PMAC: 106711437
PRAP: 110999630
PXY: 105380713
ZNE: 110837661
CEL: CELE_F28H6.3(nkat-1) CELE_R03A10.4(nkat-3)
CBR: CBG07704(Cbr-nkat-3) CBG10259
BMY: Bm1_10490
OBI: 106876997
SHX: MS3_06559
EPA: 110244036
AQU: 100641141
DDI: DDB_G0287269(ccbl)
DFA: DFA_11928(ccbl)
SMIN: v1.2.018754.t1(symbB.v1.2.018754.t1)
NGD: NGA_0728200(CCBL)
 » show all
Taxonomy
Reference
1  [PMID:14209950]
  Authors
FLAVIN M, SLAUGHTER C.
  Title
CYSTATHIONINE CLEAVAGE ENZYMES OF NEUROSPORA.
  Journal
J Biol Chem 239:2212-9 (1964)
Reference
2  [PMID:721818]
  Authors
Tateishi M, Suzuki S, Shimizu H.
  Title
Cysteine conjugate beta-lyase in rat liver. A novel enzyme catalyzing formation of thiol-containing metabolites of drugs.
  Journal
J Biol Chem 253:8854-9 (1978)
Reference
3  [PMID:4008484]
  Authors
Stevens JL
  Title
Isolation and characterization of a rat liver enzyme with both cysteine conjugate beta-lyase and kynureninase activity.
  Journal
J Biol Chem 260:7945-50 (1985)
Reference
4  [PMID:3782077]
  Authors
Stevens JL, Robbins JD, Byrd RA
  Title
A purified cysteine conjugate beta-lyase from rat kidney cytosol. Requirement for an alpha-keto acid or an amino acid oxidase for activity and identity with soluble glutamine transaminase K.
  Journal
J Biol Chem 261:15529-37 (1986)
Reference
5  [PMID:7612304]
  Authors
Gaskin PJ, Adcock HJ, Buckberry LD, Teesdale-Spittle PH, Shaw PN
  Title
The C-S lysis of L-cysteine conjugates by aspartate and alanine aminotransferase  enzymes.
  Journal
Hum Exp Toxicol 14:422-7 (1995)
DOI:10.1177/096032719501400506
Reference
6  [PMID:12137566]
  Authors
Cooper AJ, Bruschi SA, Iriarte A, Martinez-Carrion M
  Title
Mitochondrial aspartate aminotransferase catalyses cysteine S-conjugate beta-lyase reactions.
  Journal
Biochem J 368:253-61 (2002)
DOI:10.1042/BJ20020531
Reference
7  [PMID:12504794]
  Authors
Cooper AJ, Bruschi SA, Conway M, Hutson SM
  Title
Human mitochondrial and cytosolic branched-chain aminotransferases are cysteine S-conjugate beta-lyases, but turnover leads to inactivation.
  Journal
Biochem Pharmacol 65:181-92 (2003)
DOI:10.1016/S0006-2952(02)01513-7
Reference
8  [PMID:17191898]
  Authors
Natsch A, Schmid J, Flachsmann F
  Title
Identification of odoriferous sulfanylalkanols in human axilla secretions and their formation through cleavage of cysteine precursors by a C-S lyase isolated from axilla bacteria.
  Journal
Chem Biodivers 1:1058-72 (2004)
DOI:10.1002/cbdv.200490079
Reference
9  [PMID:16463021]
  Authors
Cooper AJ, Pinto JT
  Title
Cysteine S-conjugate beta-lyases.
  Journal
Amino Acids 30:1-15 (2006)
DOI:10.1007/s00726-005-0243-4
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.13
IUBMB Enzyme Nomenclature: 4.4.1.13
ExPASy - ENZYME nomenclature database: 4.4.1.13
BRENDA, the Enzyme Database: 4.4.1.13
CAS: 68652-57-3

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