KEGG   ENZYME: 4.4.1.22
Entry
EC 4.4.1.22                 Enzyme                                 
Name
S-(hydroxymethyl)glutathione synthase;
glutathione-dependent formaldehyde-activating enzyme;
Gfa;
S-(hydroxymethyl)glutathione formaldehyde-lyase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
S-(hydroxymethyl)glutathione formaldehyde-lyase (glutathione-forming)
Reaction(IUBMB)
S-(hydroxymethyl)glutathione = glutathione + formaldehyde [RN:R06982]
Reaction(KEGG)
R06982
Substrate
S-(hydroxymethyl)glutathione [CPD:C14180]
Product
glutathione [CPD:C00051];
formaldehyde [CPD:C00067]
Comment
The enzyme from Paracoccus denitrificans accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway.
History
EC 4.4.1.22 created 2005 (EC 1.2.1.1 created 1961, modified 1982, modified 2002, part transferred 2005 to EC 4.4.1.22)
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03396  S-(hydroxymethyl)glutathione synthase
Genes
MPHA105839794
SMPSMAC_08712
PANPODANSg505
TTTTHITE_49799
TMNUCRPA7_692
SSCKSPSK_01984
FGRFGSG_10776
FPUFPSE_08822
FVRFVEG_11444
FOXFOXG_12705
NHENECHADRAFT_69107
MAJMAA_09901
PLJVFPFJ_02045
VALVDBG_01395
VDAVDAG_07855
CFJCFIO01_10786
CHIGCH63R_05407
ELAUCREL1_2225
PFYPFICI_05448
PSCOLY89DRAFT_687122
ANGANI_1_14024(An02g00080)
AFVAFLA_005034
ALUCAKAW2_21597S
ACHEACHE_21301A
PCSPc20g15670
PDPPDIP_47330
TMFPMAA_051990
TRGTRUGW13939_01253
PNOSNOG_01600
PTEPTT_17259
BZECOCCADRAFT_106556
BSCCOCSADRAFT_36926
BORCOCMIDRAFT_86974
AALTCC77DRAFT_1024765
ZTRMYCGRDRAFT_87443
PFJMYCFIDRAFT_87783
FFUCLAFUR5_11225
ADPNCTC12871_00268(gfa)
XCCXCC3388
XCBXC_0776
XCAxcc-b100_0811(gfa)
XCPXCR_3724(gfa)
XCVXCV0789
XAXXACM_0733
XACXAC0735
XCIXCAW_03847
XCTJ151_00858
XCJJ158_00855
XCUJ159_00855
XCNJ169_00854
XCWJ162_00854
XCRJ163_00854
XCMJ164_00854
XCFJ172_00849
XFUXFF4834R_chr07430
XAOXAC29_03740
XOMXOO3646(XOO3646)
XOPPXO_04414
XOZBE73_20410
XSASB85_16995
XTNFD63_15635
XVEBJD12_08565
XPEBJD13_13045
XHRXJ27_19785
XPHXppCFBP6546_16605(gfa)
XVAC7V42_18175(gfa)
XANAC801_17780
XARXB05_12165
XHYFZ025_12510(gfa)
XTHG4Q83_19135(gfa)
XEUXSP_000761(gfa)
XHDLMG31886_08570(gfa)
XPRMUG10_14185(gfa)
LAREHIV01_015560(gfa)
DTXATSB10_32700
DKOI596_2122
VFIVF_A0007
VFMVFMJ11_A0021(gfa)
VSAVSAL_II0213(gfa)
PPRPBPRB1570
PDSCAY62_04965
PCQPcP3B5_24880(gfa)
PPXT1E_5519
PSBPsyr_2957
PPUUPputUW4_02203(gfa)
PKCPKB_4293(gfa)
PFKPFAS1_28165
PSILPMA3_14295
PAZEKSS91_12045(gfa)
PCAVD3880_11230
ATNFM020_06240(gfa)
SFRSfri_3436 Sfri_3438
SSESsed_4230
SPLSpea_0267 Spea_3387
SHLShal_3474 Shal_4035
SWDSwoo_0373
SVOSVI_3944(gfa)
SEURFM038_023190(gfa)
PSYPCNPT3_05390
MVSMVIS_3174(gfa)
MMAAFR932_02530(gfa)
HHUAR456_12705
HALOBWR19_16725
HCAMI4484_18760(gfa)
HSXHNO51_18715(gfa)
HSVHNO53_19035(gfa)
HTXEKK97_20970(gfa)
SALNSALB1_1489
BAMBamb_5797
BACBamMC406_5579
BPYBphyt_4238 Bphyt_5113
PACPFAZ97_30990(gfa)
AKATKWG_16070
AMIMMIM_c14300(gfa)
PARKLSG25_15680(gfa)
PVACHC248_01255(gfa)
VEIVeis_4024
MMSmma_2819
MFYHH212_09660(gfa)
MALIEYF70_29695(gfa)
MUMFCL38_19970(gfa)
MFLAGO485_25000(gfa)
MPLIE1742_23860(gfa)
URUDSM104443_01527(gfa)
BEBABWI17_19425
MLOmlr0874
MLNA9174_21485
MOPMesop_4082
MAMMesau_03843
MAMOA6B35_20710 A6B35_25035
MHUAMCHK_2549(gfa)
MJREB229_21270(gfa)
MERDEB233_18455(gfa)
MESRFGU64_02285(gfa)
AAKAA2016_2457
AMIHCO731_01953(gfa_1) CO731_02450(gfa_2)
SMESM_b20186
SMKSinme_3979
SMQSinmeB_4476
SMXSM11_pD1417
SMIBN406_06470(gfa)
SMEGC770_GR4pD1402
SMELSM2011_b20186(gfa)
SMERDU99_32400
SMDSmed_3930
RHINGR_b14630(gfa1) NGR_c19300 NGR_c20490(gfa2)
SFHSFHH103_01861 SFHH103_05888
SFDUSDA257_c31100(gfa1) USDA257_c43530(gfa2) USDA257_c54270(gfa3)
SAMESAMCFNEI73_Ch0088(gfa)
SINOSS05631_c38600
EADOV14_a1033(gfa2)
EAHFA04_27895 FA04_30330
ESJSJ05684_b47720 SJ05684_b58890
EMXFKV68_00780(gfa) FKV68_13155(gfa) FKV68_33100(gfa)
RETRHE_PF00400
RECRHECIAT_PC0000582
RELREMIM1_PF00508(gfa)
REPIE4803_PC00392(gfa)
REIIE4771_PD00410(gfa)
RLEpRL120526(gfA)
RLTRleg2_4920 Rleg2_4958
RLGRleg_4832
RLBRLEG3_07960
RLURLEG12_10940
RHLLPU83_2360(gfa)
RGARGR602_PC00929(gfa-1) RGR602_PC02286(gfa-2)
RHNAMJ98_PE00477(gfa)
RPHAAMC79_PD00607(gfa)
RHXAMK02_PD00340(gfa)
RHKKim5_PC00370(gfa)
REZAMJ99_PD00477(gfa)
RJGCCGE525_10885(gfa)
RGRFZ934_22410(gfa)
RADCO657_27365(gfa)
RIIFFM53_027555(gfa)
RHIDFFM81_024520(gfa)
RBQJ2J99_23700(gfa)
RLNJ0663_23815(gfa)
RBANJ2J98_23015(gfa)
RRGJ3P73_26630(gfa)
RHTNT26_3258(gfa)
RFVRFYW14_03122
SHZshn_31780
SZOK8M09_22425(gfa)
KAIK32_22190(gfa)
BJAblr6216(blr6216)
BJUBJ6T_34850
BJPRN69_16905
BRABRADO5487(gfa)
BBTBBta_5971(gfa)
BRSS23_20790
AOLS58_22210
BRCBCCGELA001_26715
BRADBF49_5354 BF49_5366
BICLMTR13_13680
BROBRAD285_1746(gfa)
BRKCWS35_16195(gfa) CWS35_32580(gfa)
BOTCIT37_23150(gfa)
BRQCIT40_23255(gfa)
BGQX265_11175(gfa)
BGZXH91_10400(gfa)
BSYMCIT39_22250(gfa)
BBETF8237_25275(gfa)
BARHWN72_34695(gfa)
BVZBRAD3257_2931(gfa)
BELBE61_38370
BDGLPJ38_32960(gfa)
BSEPHAP48_0048215(gfa)
BQBJ4P68_0007425(gfa)
RPCRPC_0100
RPERPE_4679
TRBHB776_18780(gfa)
MOCBB934_29595
BBARRHAL1_00658(gfa) RHAL1_01784
BRND1F64_20380(gfa)
NOHG5V57_12155(gfa)
ACUTMRB58_02640(gfa)
YPACCEW88_21860(gfa)
YANAYJ57_23665
TPROGa0080559_TMP1801
PABYGa0080574_TMP304
SAGUCDO87_25880(gfa)
OCDFHY55_11710(gfa)
RMHLVO79_17155(gfa)
MALUKU6B_56540(gfa)
PALXGQA70_00385(gfa)
RSPRSP_2575
RSHRsph17029_1233
RSQRsph17025_1948
RSKRSKD131_0887
CAZTLV780_08635(gfa)
PDEPden_0015
PAMIJCM7686_0085
PYEA6J80_09895(gfa)
PCONB0A89_05655
PZHCX676_13675(gfa)
PAROCUV01_07510(gfa)
PAMNJCM7685_0202(gfa)
PMUTDPM13_08095(gfa)
PARSDRW48_11880(gfa)
PARREOJ32_02150(gfa)
PAAKFIU66_13740(gfa)
PKDF8A10_00210(gfa)
PPANESD82_21255(gfa)
PLIAE4191_03490(gfa) E4191_06110(gfa) E4191_14885(gfa)
PSANHGN31_08085(gfa)
PMAUCP157_00050(gfa)
DAAAKL17_0665
GFUKM031_18915(gfa)
HMLHmaOT1_09240(gfa)
PPRUFDP22_18560(gfa)
PAMOBAR1_01170(gfa)
PGVSL003B_0765
AMAQGO499_15675(gfa)
SPHAD3Y57_02580(gfa)
ACRAcry_2793
AMVACMV_31250(gfa)
RMTIAI58_19635(gfa)
SWFE3E12_02505(gfa)
AZZDEW08_23520(gfa)
SKTIGS68_32150(gfa)
SMUCJL100_034250(gfa)
SROEJL101_028910(gfa)
HTQFRZ44_12310(gfa)
HADHFRZ61_12390(gfa)
MAGQMGMAQ_0499(gfa)
AFRAFE_0696(gfa)
AFELferr_0844
AFIAcife_0709
AFJAFERRID_19300
PCXLPB68_21510
SIVSSIL_2807
PLLI858_008940
PHCBBI08_11300
CRNCAR_c23670(gfa)
CAWQ783_10630
CVDLHA31_07905(gfa)
 » show all
Reference
1  [PMID:11741920]
  Authors
Goenrich M, Bartoschek S, Hagemeier CH, Griesinger C, Vorholt JA
  Title
A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy.
  Journal
J Biol Chem 277:3069-72 (2002)
DOI:10.1074/jbc.C100579200
  Sequence
[pde:Pden_0015]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.22
IUBMB Enzyme Nomenclature: 4.4.1.22
ExPASy - ENZYME nomenclature database: 4.4.1.22
UM-BBD (Biocatalysis/Biodegradation Database): 4.4.1.22
BRENDA, the Enzyme Database: 4.4.1.22
CAS: 425642-27-9

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