Entry
Name
L-cysteine desulfidase;
L-cysteine desulfhydrase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
L-cysteine sulfide-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-cysteine + H2O = sulfide + NH3 + pyruvate (overall reaction) [RN:
R00782 ];
(1a) L-cysteine = 2-aminoprop-2-enoate + sulfide;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
Product
Comment
The enzyme from the archaeon Methanocaldococcus jannaschii contains a [4Fe-4S] cluster and is specific for L-cysteine (cf. EC
4.4.1.1 , cystathionine gamma-lyase). It cleaves a carbon-sulfur bond releasing sulfide and the unstable enamine product 2-aminoprop-2-enoate that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The same reaction can also be catalysed by some pyridoxal-phosphate proteins (cf. EC
4.4.1.1 , cystathionine gamma-lyase).
History
EC 4.4.1.28 created 2014
Pathway
ec00270 Cysteine and methionine metabolism
Orthology
K22207 L-cysteine desulfhydrase
Genes
LPIC : 129277655 129277813
PFLL : 139114593 139114641 139114642 139142731
MMER : 123530484 123564270
RPHI : 132728620 132758918
DPOL : 127857968 127863521
MAEA : 128223879 128224566 128224925
AQU : 100634704 100642116 105315073
ATH : AT3G62130(LCD) AT5G26600
CSAT : 104699663 104706986 104736694 104749103 104788528
EUS : EUTSA_v10004144mg EUTSA_v10005965mg
BNA : 106348582 106348715 106353308 111211374
THJ : 104802117 104802771 104818753
CIC : CICLE_v10011696mg CICLE_v10013565mg CICLE_v10013630mg CICLE_v10020107mg
PVY : 116136618 116138149 116138765 116143214
MINC : 123206460 123223800
GRA : 105761151 105790386 105802893
GHI : 107912242 107916839 107923624 107927291 107955413 121220212
GAB : 108467707 108474143 108479955
HSYR : 120135361 120136396 120167386 120189662
RARG : 115756163 115756241
GMX : 100778443 100786129 100787528 100793745 100796732
GSJ : 114369965 114377994 114400557 114403152 114407515
PVU : 137814880 137830114 137830159
VRA : 106754861 106757980 106769802 106776815
VAR : 108324939 108325888 108326803
VUN : 114186808 114191967 114192303 114192796
VUM : 124824959 124840448 124840450 124845597
CCAJ : 109794318 109797560 109807724
APRC : 113862943 113872820
MTR : 11407630 11432056 25485791
TPRA : 123886010 123891005 123902167
CAM : 101489071 101497392 101500508
PSAT : 127091461 127091913 127132494
VVO : 131610505 131610506 131610508 131643979 131644015
ADU : 107465010 107465126 107492236
AIP : 107618418 107619001 107645985
AHF : 112695619 112710614 112710680 112757987 112775364 112775746
LANG : 109328859 109331952 109336357
PCIN : 129306495 129310894
QSA : O6P43_007321 O6P43_007377 O6P43_010979 O6P43_010981
FVE : 101297568 101297861 101300436
AANS : 126799732 126799735
RCN : 112191781 112192553 112203324 112203626
PPER : 18771931 18772177 18774311 18774576 18775399
PMUM : 103344505 103344506 103344507
PAVI : 110770561 110770576 110770583 110770601 110770603
PDUL : 117630572 117631631
MDM : 103419434 103450812 103450813
MSYL : 126594086 126594087 126618483
PXB : 103928254 103928271 103955312 103955314 103955848
ZJU : 107411685 107411704 107426174
CSAV : 115713573 115719358 133032718
CMAX : 111468593 111493740
CMOS : 111444191 111450311
CPEP : 111799131 111804730
MEAN : 126673965 126688300
HBR : 110633045 110653717 110661721
MESC : 110613758 110614855 110621785 110623107
POP : 18098456 7465209 7481444 7495177
PEU : 105112089 105125214 105135713 105136544
PALZ : 118034942 118041124 118044488 118060877
PNZ : 133670072 133682337 133692531 133695027
JRE : 108987902 108987909 109007781
CILL : 122314732 122316054 122316722
CAVE : 132169193 132170814 132172382
AGLU : 133851280 133851405
QSU : 112002990 112003723 112007563 136065768
QLO : 115968171 115972135 115981667
SPEN : 107008434 107018829
SSTN : 125854924 125873337
SDUL : 129880172 129887072
CANN : 107840630 107870915
NTA : 107762255 107809393 107827141 107831265
INI : 109164575 109164610 109182896
SIND : 105162984 105169003
EGT : 105955674 105960205 105961793 105972775 105978147
SSPL : 121746386 121753395 121756826
SMIL : 130989755 131007216
SHIS : 125185700 125214347
APAN : 127258917 127260897 127262664
ECAD : 122594530 122606783
CCAV : 112518257 112525541
CSIN : 114268177 114286807
AEW : 130751028 130758500 130780024 130784460
TSS : 122647796 122647799 122647800 122672570
PSOM : 113333345 113334886 113347054 113358019
OSA : 4325155(L-CDes1) 4325157
DOSA : Os01g0290100 Os01g0290600
TAES : 123061159 123069953 123078313
MFLO : 136455969 136458129 136458130 136458131
SITA : 101784048 101784864
PVIR : 120672988 120676860 120706618 120706619
PHAI : 112893430 112894567
PAUA : 133900024 133910541
MUS : 103999773 135606069 135626582
ZOF : 122007934 122017527 122019783 122023459 122051821 122056349
MSIN : 131225829 131245229
NCOL : 116262202 116263955
SMO : SELMODRAFT_232064 SELMODRAFT_232793 SELMODRAFT_413706
» show all
Taxonomy
Reference
Authors
Tchong SI, Xu H, White RH
Title
L-cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.28
ExPASy - ENZYME nomenclature database: 4.4.1.28