KEGG   ENZYME: 4.4.1.28
Entry
EC 4.4.1.28                 Enzyme                                 
Name
L-cysteine desulfidase;
L-cysteine desulfhydrase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
L-cysteine sulfide-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-cysteine + H2O = sulfide + NH3 + pyruvate (overall reaction) [RN:R00782];
(1a) L-cysteine = 2-aminoprop-2-enoate + sulfide;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
R00782
Substrate
L-cysteine [CPD:C00097];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
sulfide [CPD:C00283];
NH3 [CPD:C00014];
pyruvate [CPD:C00022];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
The enzyme from the archaeon Methanocaldococcus jannaschii contains a [4Fe-4S] cluster and is specific for L-cysteine (cf. EC 4.4.1.1, cystathionine gamma-lyase). It cleaves a carbon-sulfur bond releasing sulfide and the unstable enamine product 2-aminoprop-2-enoate that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The same reaction can also be catalysed by some pyridoxal-phosphate proteins (cf. EC 4.4.1.1, cystathionine gamma-lyase).
History
EC 4.4.1.28 created 2014
Pathway
ec00270  Cysteine and methionine metabolism
ec01100  Metabolic pathways
Orthology
K20021  L-cysteine desulfidase
K22207  L-cysteine desulfhydrase
Genes
SKO100366607
PMAX117319314
MMER123530484 123564270
AQU100634704 100642116 105315073
ATHAT3G62130(LCD) AT5G26600
ALY9308301 9314504
CRB17882306 17885855
CSAT104699663 104706986 104736694 104749103 104788528
EUSEUTSA_v10004144mg EUTSA_v10005965mg
BRP103830344 103874481
BNA106348582 106348715 106353308 111211374
BOE106300027 106306993
RSZ108817289 108833893 108854538
THJ104802117 104802771 104818753
CPAP110815337
CIT102626410 102628307
CICCICLE_v10011696mg CICLE_v10013565mg CICLE_v10013630mg CICLE_v10020107mg
PVY116136618 116138149 116138765 116143214
MINC123206460 123223800
TCC18600637 18613807
GRA105761151 105790386 105802893
GHI107912242 107916839 107923624 107927291 107955413 121220212
GAB108467707 108474143 108479955
DZI111295641 111314027
EGR104424621 104426923 104431440
GMX100778443 100786129 100787528 100793745 100796732
GSJ114369965 114377994 114400557 114403152 114407515
PVUPHAVU_001G222200g PHAVU_007G204900g PHAVU_007G209500g
VRA106754861 106757980 106769802 106776815
VAR108324939 108325888 108326803
VUN114186808 114191967 114192303 114192796
CCAJ109794318 109797560 109807724
APRC113862943 113872820
MTR11407630 11432056 25485791
CAM101489071 101497392 101500508
LJALj0g3v0131109.1(Lj0g3v0131109.1) Lj0g3v0330309.1(Lj0g3v0330309.1) Lj1g3v4807370.1(Lj1g3v4807370.1)
ADU107465010 107465126 107492236
AIP107618418 107619001 107645985
AHF112695619 112710614 112710680 112757987 112775364 112775746
LANG109328859 109331952 109336357
FVE101297568 101297861 101300436
RCN112191781 112192553 112203324 112203626
PPER18771931 18772177 18774311 18774576 18775399
PMUM103344505 103344506 103344507
PAVI110770561 110770576 110770583 110770601 110770603
PDUL117630572 117631631
MDM103419434 103450812 103450813
PXB103928254 103928271 103955312 103955314 103955848
ZJU107411679 107411685 107411704 107426174
MNT21397044 21405460
CSV101221312
CMO103493954
BHJ120079983 120085022
MCHA111023288
CMAX111468593 111493740
CMOS111444191 111450311
CPEP111799131 111804730
RCU8265875 8274960
JCU105642703 105647374
HBR110633045 110653717 110661721
MESC110613758 110614855 110621785 110623107
POP18098456 7465209 7481444 7495177
PEU105112089 105125214 105135713 105136544
PALZ118034942 118041124 118044488 118060877
JRE108987902 108987909 109007781
QSU112002990 112003723 112007563
QLO115968171 115972135 115981667
TWL119982925 120009424
VVI100243446 100244282
VRI117919398 117930146
SLY101258894 101267603
SPEN107008434 107018829
SOT102586162 102604167
SSTN125854924 125873337
CANN107840630 107870915
NTA107762255 107809393 107827141 107831265
NSY104215557 104216480
NTO104111542 104120808
NAU109224794 109243283
INI109164575 109164610 109182896
ITR116026046 116031508
SIND105162984 105169003
OEU111369624 111384853
EGT105955674 105960205 105961793 105972775 105978147
SSPL121746386 121753395 121756826
HAN110877155 110884362
ECAD122594530 122606783
LSV111908772 111915341
CCAV112518257 112525541
DCR108204925 108215311
CSIN114268177 114286807
BVG104890630
SOE110797240
CQI110701242 110736569
NNU104586046 104607171
MING122057865
TSS122647796 122647799 122647800 122672570
PSOM113333345 113334886 113347054 113358019
NCOL116262202 116263955
OSA4325155 4325157
DOSAOs01t0290100-01(Os01g0290100) Os01t0290600-00(Os01g0290600)
OBR102718401 102718687
BDI100844459
ATS109769734 109784901
TDC119268049 119276115
TAES123061159 123069953 123078313
TUA125543999
SBI110433861 8078148
ZMA100193096 100284775
SITA101784048 101784864
SVS117857306 117857307
PVIR120672988 120676860 120706618 120706619
PHAI112893430 112894567
PDA103695778 103722110
EGU105035146 105035494
MUS103971727 103975040 103999773
DCT110101195
PEQ110029309
AOF109846945
ATR18430133
SMOSELMODRAFT_232064 SELMODRAFT_232793 SELMODRAFT_413706
PPP112283842 112285332
CRECHLRE_07g337000v5
VCNVOLCADRAFT_95613
CSLCOCSUDRAFT_40034
MISMICPUN_113236(LOLT)
FVRFVEG_17134
MBRMONBRDRAFT_10380
SREPTSG_02780
AAFAURANDRAFT_64603
LIFLINJ_28_0200
LDOLDBPK_280200
MJAMJ_1025
MFEMefer_1194
MVUMetvu_0638
MFSMFS40622_1642
MIFMetin_1014
MJHJH146_0918
MESGMLAUSG7_0847
MESAMLASG1_0908
MIGMetig_0767
MMPMMP1468
MMQMmarC5_0110
MMXMmarC6_1205
MMZMmarC7_0713
MMDGYY_08175
MMAKMMKA1_16920
MMAOMMOS7_15750
MMADMMJJ_13710
 » show all
Reference
1  [PMID:15683250]
  Authors
Tchong SI, Xu H, White RH
  Title
L-cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide.
  Journal
Biochemistry 44:1659-70 (2005)
DOI:10.1021/bi0484769
  Sequence
[mja:MJ_1025]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.28
IUBMB Enzyme Nomenclature: 4.4.1.28
ExPASy - ENZYME nomenclature database: 4.4.1.28
BRENDA, the Enzyme Database: 4.4.1.28

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