KEGG   ENZYME: 4.4.1.31
Entry
EC 4.4.1.31                 Enzyme                                 

Name
phycoerythrocyanin alpha-cysteine-84 phycoviolobilin lyase/isomerase;
pecE (gene name);
pecF (gene name);
phycoviolobilin phycoerythrocyanin-alpha84-cystein-lyase;
PecE/PecF;
PEC-Cys-R84 PCB lyase/isomerase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
[phycoerythrocyanin alpha-subunit]-Cys84-phycoviolobilin:(2R,3E)-phycocyanobilin lyase/isomerase
Reaction(IUBMB)
[phycoerythrocyanin alpha-subunit]-Cys84-phycoviolobilin = apo-[phycoerythrocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
Substrate
[phycoerythrocyanin alpha-subunit]-Cys84-phycoviolobilin
Product
apo-[phycoerythrocyanin alpha-subunit];
(2R,3E)-phycocyanobilin
Comment
The enzyme, characterized from the cyanobacteria Nostoc sp. PCC 7120 and Mastigocladus laminosus, catalyses the covalent attachment of the phycobilin chromophore phycocyanobilin to cysteine 84 of the beta subunit of the phycobiliprotein phycoerythrocyanin and its isomerization to phycoviolobilin.
History
EC 4.4.1.31 created 2015
Orthology
K02631  phycoerythrocyanin alpha-cysteine-84 phycoviolobilin lyase/isomerase subunit PecE
K02632  phycoerythrocyanin alpha-cysteine-84 phycoviolobilin lyase/isomerase subunit PecF
Genes
OXY: HCG48_09695 HCG48_09700
ANA: alr0526(pecE) alr0527(pecF)
NOS: Nos7107_3147 Nos7107_3148
NOP: Nos7524_4171 Nos7524_4172
AVA: Ava_2928 Ava_2929
NAZ: Aazo_0293 Aazo_0294
ACY: Anacy_2029 Anacy_2030
ANN: EH233_10495 EH233_10500
CSG: Cylst_0749 Cylst_0750
CALO: Cal7507_3697 Cal7507_3698
 » show all
Reference
1  [PMID:7759546]
  Authors
Jung LJ, Chan CF, Glazer AN
  Title
Candidate genes for the phycoerythrocyanin alpha subunit lyase. Biochemical analysis of pecE and pecF interposon mutants.
  Journal
J Biol Chem 270:12877-84 (1995)
DOI:10.1074/jbc.270.21.12877
Reference
2  [PMID:10708746]
  Authors
Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H
  Title
Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon.
  Journal
FEBS Lett 469:9-13 (2000)
DOI:10.1016/S0014-5793(00)01245-X
Reference
3  [PMID:11591166]
  Authors
Storf M, Parbel A, Meyer M, Strohmann B, Scheer H, Deng MG, Zheng M, Zhou M, Zhao KH
  Title
Chromophore attachment to biliproteins: specificity of PecE/PecF, a lyase-isomerase for the photoactive 3(1)-cys-alpha 84-phycoviolobilin chromophore of phycoerythrocyanin.
  Journal
Biochemistry 40:12444-56 (2001)
DOI:10.1021/bi010776s
Reference
4  [PMID:12230566]
  Authors
Zhao KH, Wu D, Wang L, Zhou M, Storf M, Bubenzer C, Strohmann B, Scheer H
  Title
Characterization of phycoviolobilin phycoerythrocyanin-alpha 84-cystein-lyase-(isomerizing) from Mastigocladus laminosus.
  Journal
Eur J Biochem 269:4542-50 (2002)
DOI:10.1046/j.1432-1033.2002.03148.x
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.31
IUBMB Enzyme Nomenclature: 4.4.1.31
ExPASy - ENZYME nomenclature database: 4.4.1.31
BRENDA, the Enzyme Database: 4.4.1.31

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