KEGG   ENZYME: 4.4.1.32
Entry
EC 4.4.1.32                 Enzyme                                 
Name
C-phycocyanin alpha-cysteine-84 phycocyanobilin lyase;
cpcE (gene name);
cpcF (gene name)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin:(2R,3E)-phycocyanobilin lyase
Reaction(IUBMB)
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
Substrate
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin
Product
apo-[C-phycocyanin alpha-subunit];
(2R,3E)-phycocyanobilin
Comment
The enzyme, characterized from the cyanobacterium Synechococcus elongatus PCC 7942, catalyses the covalent attachment of the phycobilin chromophore phycocyanobilin to cysteine 84 of the alpha subunit of the phycobiliprotein C-phycocyanin.
History
EC 4.4.1.32 created 2015
Orthology
K02288  phycocyanobilin lyase subunit alpha
K02289  phycocyanobilin lyase subunit beta
Genes
CMECYME_CMJ043C CYME_CMJ044C
GSLGasu_13890 Gasu_61960
CCPCHC_T00009149001
BBAEFRD01_12790
SYNsll1051(cpcF) slr1878(cpcE)
SYZMYO_111170(cpcE) MYO_1760(cpcF)
SYYSYNGTS_0076(cpcF) SYNGTS_1107(cpcE)
SYTSYNGTI_0076(cpcF) SYNGTI_1107(cpcE)
SYSSYNPCCN_0076(cpcF) SYNPCCN_1106(cpcE)
SYQSYNPCCP_0076(cpcF) SYNPCCP_1106(cpcE)
SYJD082_28370(cpcE) D082_35050(cpcF)
SYOC7I86_00395 C7I86_05750
SYCsyc0493_c(cpcF) syc0494_c(cpcE)
SYUM744_11405 M744_11410
SYFSynpcc7942_1054 Synpcc7942_1055
SYESyncc9902_1911 Syncc9902_1912
SYGsync_0485(cpcF) sync_0486(cpcE)
SYRSynRCC307_2066(rpcE) SynRCC307_2067(rpcF)
SYXSynWH7803_0476(rpcF) SynWH7803_0477(rpcE)
SYPSYNPCC7002_A2213(cpcE) SYNPCC7002_A2214(cpcF)
CYACYA_0216(cpcF) CYA_0217(cpcE-1) CYA_2040(cpcE-2)
CYBCYB_0942(cpcE-1) CYB_0943(cpcF) CYB_2736(cpcE-2)
SYNESyn6312_2504 Syn6312_2505
SYNPSyn7502_03299 Syn7502_03300
SYNRKR49_11160 KR49_11165
SYNDKR52_00320 KR52_00325
SYHSyncc8109_0435(rpcF) Syncc8109_0436(rpcE)
SYNWSynWH8103_02329(rpcE) SynWH8103_02330(rpcF)
SYVAWQ23_11055 AWQ23_11060
SYLAWQ21_11200 AWQ21_11205
SYNCCB0101_10390 CB0101_10395
SYNYBM449_08880 BM449_08885
DSLDacsa_0415 Dacsa_0532
CGCCyagr_1640 Cyagr_1641
CYICBM981_1808(rpcE) CBM981_1809(cpcF)
CMPCha6605_1301 Cha6605_3627
AMRAM1_C0118(cpcE) AM1_C0272(cpcF)
WNAKA717_07135 KA717_14545
TVNNIES2134_118880(cpcE) NIES2134_118890(cpcF)
SLWBRW62_10680 BRW62_10685
TELtlr1961(cpcE) tlr1962(cpcF)
THNNK55_06500(cpcE) NK55_06505(cpcF)
THECFFX45_03420 FFX45_03425
LEPLepto7376_2325 Lepto7376_2326
LENLEP3755_55240 LEP3755_55250
LETO77CONTIG1_02223(cpcF) O77CONTIG1_02224(cpcE)
LBOLBWT_26800 LBWT_26810
KOVK9N68_10120 K9N68_18785
LSKJ5X98_11865 J5X98_16875
HHGXM38_016360(cpcF) XM38_016370(cpcE)
PSEUPse7367_2386 Pse7367_2387 Pse7367_3924 Pse7367_3925
PSERABRG53_2398(cpcF) ABRG53_2399(cpcE)
THEUHPC62_03215 HPC62_03220
GLPGlo7428_4601 Glo7428_4602
GENGM3709_565 GM3709_771
GEEGM3708_3361 GM3708_665
CHONNIES4102_25470 NIES4102_32740
MARMAE_27950(cpcF) MAE_27970(cpcE)
MPKVL20_5166 VL20_5175
MIQB5D77_00715 B5D77_00725
MVZmyaer102_04100(cpcF) myaer102_04190(cpcE)
CANCyan10605_1973 Cyan10605_2164
CSNCyast_0723 Cyast_1932
CYLAA637_00365(cpcE) AA637_07775(cpcF)
HAOPCC7418_1993 PCC7418_2470
ENNFRE64_08540 FRE64_10565
CYTcce_2308(cpcF) cce_2656(cpcE)
CWACwatDRAFT_3431 CwatDRAFT_6054
CYPPCC8801_3073 PCC8801_3079
CYHCyan8802_3041 Cyan8802_3047
CYCPCC7424_0156 PCC7424_0661
CYJCyan7822_1647 Cyan7822_2446
CYNCyan7425_1691 Cyan7425_1692
TERTery_5046 Tery_5047
ARPNIES39_D02000(cpcF) NIES39_D02010(cpcE)
PAGHNIES204_12100(cpcF) NIES204_12110(cpcE)
PPSUNO713_00391(cpcE) NO713_00392(cpcF1)
OXYHCG48_11030 HCG48_11035
MVAGD0A34_14270 D0A34_14275
LFSHFV01_19980 HFV01_19985
MICMic7113_2593 Mic7113_2594
GEIGEI7407_3505 GEI7407_3506
OACOscil6304_1239 Oscil6304_1240
ONIOsc7112_2003 Osc7112_2004
MPROBJP34_30445 BJP34_30450
PHORJWS08_03850 JWS08_03855
PYHNEA10_07450 NEA10_07455
CEPCri9333_1814 Cri9333_1815
GVIglr1268(cpcE) glr1269(cpcF)
GLJGKIL_4303(cpcE) GKIL_4304(cpcF)
GMLISF26_01135 ISF26_01140
ANAalr0532(cpcE) alr0533(cpcF)
NPUNpun_F5294 Npun_F5295
NOSNos7107_3140 Nos7107_3141
NOPNos7524_4177 Nos7524_4178
NONNOS3756_52570(cpcE) NOS3756_52580(cpcF)
NFLCOO91_01509 COO91_01510
NOECLI64_20470 CLI64_20475
NSHGXM_03737 GXM_03738
NEDHUN01_13320 HUN01_13325
AVAAva_2934 Ava_2935
NAZAazo_3484 Aazo_3485
ANBANA_C11072(cpcE) ANA_C11073(cpcF)
ACYAnacy_1900 Anacy_1901
AWAAA650_07335 AA650_07340
ANNEH233_10465 EH233_10470
CSGCylst_0755 Cylst_0756
RSINB6N60_02118 B6N60_02119
CALOCal7507_3703 Cal7507_3704
CALTCal6303_3400 Cal6303_3401
CALHIJ00_13725 IJ00_13730
CALNNIES2098_62620 NIES2098_62630
RIVRiv7116_3560 Riv7116_3561
FISFIS3754_26590 FIS3754_26600
NSPBMF81_02855(cpcF) BMF81_02856(cpcE)
NSPHBDGGKGIB_01429(cpcF) BDGGKGIB_01430(cpcE)
DOUBMF77_00697(cpcE) BMF77_00698(cpcF)
DFSHGD76_14855 HGD76_14860
DHTNG743_18555 NG743_18560
CCURIAR63_12805 IAR63_12810
CRKL3I90_11400 L3I90_11405
AFLOHEQ12_08790 HEQ12_08795
TOQHCG51_27020 HCG51_27025
NCNBZZ01_04070 BZZ01_04075
CTHEChro_3411 Chro_3412
PLPPle7327_2230 Ple7327_3797
SCSSta7437_0254 Sta7437_0255
STANSTA3757_01070 STA3757_01080
PCORKS4_01920
 » show all
Reference
1  [PMID:1495995]
  Authors
Fairchild CD, Zhao J, Zhou J, Colson SE, Bryant DA, Glazer AN
  Title
Phycocyanin alpha-subunit phycocyanobilin lyase.
  Journal
Proc Natl Acad Sci U S A 89:7017-21 (1992)
DOI:10.1073/pnas.89.15.7017
Reference
2  [PMID:8132596]
  Authors
Fairchild CD, Glazer AN
  Title
Oligomeric structure, enzyme kinetics, and substrate specificity of the phycocyanin alpha subunit phycocyanobilin lyase.
  Journal
J Biol Chem 269:8686-94 (1994)
Reference
3  [PMID:7524727]
  Authors
Bhalerao RP, Lind LK, Gustafsson P
  Title
Cloning of the cpcE and cpcF genes from Synechococcus sp. PCC 6301 and their inactivation in Synechococcus sp. PCC 7942.
  Journal
Plant Mol Biol 26:313-26 (1994)
DOI:10.1007/BF00039542
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.32
IUBMB Enzyme Nomenclature: 4.4.1.32
ExPASy - ENZYME nomenclature database: 4.4.1.32
BRENDA, the Enzyme Database: 4.4.1.32

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