KEGG   ENZYME: 4.4.1.32
Entry
EC 4.4.1.32                 Enzyme                                 
Name
C-phycocyanin alpha-cysteine-84 phycocyanobilin lyase;
cpcE (gene name);
cpcF (gene name)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin:(2R,3E)-phycocyanobilin lyase
Reaction(IUBMB)
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
Substrate
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin
Product
apo-[C-phycocyanin alpha-subunit];
(2R,3E)-phycocyanobilin
Comment
The enzyme, characterized from the cyanobacterium Synechococcus elongatus PCC 7942, catalyses the covalent attachment of the phycobilin chromophore phycocyanobilin to cysteine 84 of the alpha subunit of the phycobiliprotein C-phycocyanin.
History
EC 4.4.1.32 created 2015
Orthology
K02288  phycocyanobilin lyase subunit alpha
K02289  phycocyanobilin lyase subunit beta
Genes
CME: CYME_CMJ043C CYME_CMJ044C
GSL: Gasu_13890 Gasu_61960
CCP: CHC_T00009149001
BBAE: FRD01_12790
SYN: sll1051(cpcF) slr1878(cpcE)
SYZ: MYO_111170(cpcE) MYO_1760(cpcF)
SYY: SYNGTS_0076(cpcF) SYNGTS_1107(cpcE)
SYT: SYNGTI_0076(cpcF) SYNGTI_1107(cpcE)
SYS: SYNPCCN_0076(cpcF) SYNPCCN_1106(cpcE)
SYQ: SYNPCCP_0076(cpcF) SYNPCCP_1106(cpcE)
SYJ: D082_28370(cpcE) D082_35050(cpcF)
SYO: C7I86_00395 C7I86_05750
SYC: syc0493_c(cpcF) syc0494_c(cpcE)
SYU: M744_11405 M744_11410
SYF: Synpcc7942_1054 Synpcc7942_1055
SYE: Syncc9902_1911 Syncc9902_1912
SYG: sync_0485(cpcF) sync_0486(cpcE)
SYR: SynRCC307_2066(rpcE) SynRCC307_2067(rpcF)
SYX: SynWH7803_0476(rpcF) SynWH7803_0477(rpcE)
SYP: SYNPCC7002_A2213(cpcE) SYNPCC7002_A2214(cpcF)
CYA: CYA_0216(cpcF) CYA_0217(cpcE-1) CYA_2040(cpcE-2)
CYB: CYB_0942(cpcE-1) CYB_0943(cpcF) CYB_2736(cpcE-2)
SYNE: Syn6312_2504 Syn6312_2505
SYNP: Syn7502_03299 Syn7502_03300
SYNR: KR49_11160 KR49_11165
SYND: KR52_00320 KR52_00325
SYH: Syncc8109_0435(rpcF) Syncc8109_0436(rpcE)
SYNW: SynWH8103_02329(rpcE) SynWH8103_02330(rpcF)
SYV: AWQ23_11055 AWQ23_11060
SYL: AWQ21_11200 AWQ21_11205
SYNC: CB0101_10390 CB0101_10395
SYNY: BM449_08880 BM449_08885
DSL: Dacsa_0415 Dacsa_0532
CGC: Cyagr_1640 Cyagr_1641
CYI: CBM981_1808(rpcE) CBM981_1809(cpcF)
CMP: Cha6605_1301 Cha6605_3627
AMR: AM1_C0118(cpcE) AM1_C0272(cpcF)
WNA: KA717_07135 KA717_14545
TVN: NIES2134_118880(cpcE) NIES2134_118890(cpcF)
SLW: BRW62_10680 BRW62_10685
TEL: tlr1961(cpcE) tlr1962(cpcF)
THN: NK55_06500(cpcE) NK55_06505(cpcF)
THEC: FFX45_03420 FFX45_03425
LEP: Lepto7376_2325 Lepto7376_2326
LEN: LEP3755_55240 LEP3755_55250
LET: O77CONTIG1_02223(cpcF) O77CONTIG1_02224(cpcE)
LBO: LBWT_26800 LBWT_26810
KOV: K9N68_10120 K9N68_18785
LSK: J5X98_11865 J5X98_16875
HHG: XM38_016360(cpcF) XM38_016370(cpcE)
PSEU: Pse7367_2386 Pse7367_2387 Pse7367_3924 Pse7367_3925
PSER: ABRG53_2398(cpcF) ABRG53_2399(cpcE)
THEU: HPC62_03215 HPC62_03220
GLP: Glo7428_4601 Glo7428_4602
GEN: GM3709_565 GM3709_771
GEE: GM3708_3361 GM3708_665
CHON: NIES4102_25470 NIES4102_32740
MAR: MAE_27950(cpcF) MAE_27970(cpcE)
MPK: VL20_5166 VL20_5175
MIQ: B5D77_00715 B5D77_00725
MVZ: myaer102_04100(cpcF) myaer102_04190(cpcE)
CAN: Cyan10605_1973 Cyan10605_2164
CSN: Cyast_0723 Cyast_1932
CYL: AA637_00365(cpcE) AA637_07775(cpcF)
HAO: PCC7418_1993 PCC7418_2470
ENN: FRE64_08540 FRE64_10565
CYT: cce_2308(cpcF) cce_2656(cpcE)
CWA: CwatDRAFT_3431 CwatDRAFT_6054
CYP: PCC8801_3073 PCC8801_3079
CYH: Cyan8802_3041 Cyan8802_3047
CYC: PCC7424_0156 PCC7424_0661
CYJ: Cyan7822_1647 Cyan7822_2446
CYN: Cyan7425_1691 Cyan7425_1692
TER: Tery_5046 Tery_5047
ARP: NIES39_D02000(cpcF) NIES39_D02010(cpcE)
PAGH: NIES204_12100(cpcF) NIES204_12110(cpcE)
PPSU: NO713_00391(cpcE) NO713_00392(cpcF1)
OXY: HCG48_11030 HCG48_11035
MVAG: D0A34_14270 D0A34_14275
LFS: HFV01_19980 HFV01_19985
MIC: Mic7113_2593 Mic7113_2594
GEI: GEI7407_3505 GEI7407_3506
OAC: Oscil6304_1239 Oscil6304_1240
ONI: Osc7112_2003 Osc7112_2004
MPRO: BJP34_30445 BJP34_30450
PHOR: JWS08_03850 JWS08_03855
PYH: NEA10_07450 NEA10_07455
CEP: Cri9333_1814 Cri9333_1815
GVI: glr1268(cpcE) glr1269(cpcF)
GLJ: GKIL_4303(cpcE) GKIL_4304(cpcF)
GML: ISF26_01135 ISF26_01140
ANA: alr0532(cpcE) alr0533(cpcF)
NPU: Npun_F5294 Npun_F5295
NOS: Nos7107_3140 Nos7107_3141
NOP: Nos7524_4177 Nos7524_4178
NON: NOS3756_52570(cpcE) NOS3756_52580(cpcF)
NFL: COO91_01509 COO91_01510
NOE: CLI64_20470 CLI64_20475
NSH: GXM_03737 GXM_03738
NED: HUN01_13320 HUN01_13325
AVA: Ava_2934 Ava_2935
NAZ: Aazo_3484 Aazo_3485
ANB: ANA_C11072(cpcE) ANA_C11073(cpcF)
ACY: Anacy_1900 Anacy_1901
AWA: AA650_07335 AA650_07340
ANN: EH233_10465 EH233_10470
CSG: Cylst_0755 Cylst_0756
RSIN: B6N60_02118 B6N60_02119
CALO: Cal7507_3703 Cal7507_3704
CALT: Cal6303_3400 Cal6303_3401
CALH: IJ00_13725 IJ00_13730
CALN: NIES2098_62620 NIES2098_62630
RIV: Riv7116_3560 Riv7116_3561
FIS: FIS3754_26590 FIS3754_26600
NSP: BMF81_02855(cpcF) BMF81_02856(cpcE)
NSPH: BDGGKGIB_01429(cpcF) BDGGKGIB_01430(cpcE)
DOU: BMF77_00697(cpcE) BMF77_00698(cpcF)
DFS: HGD76_14855 HGD76_14860
DHT: NG743_18555 NG743_18560
CCUR: IAR63_12805 IAR63_12810
CRK: L3I90_11400 L3I90_11405
AFLO: HEQ12_08790 HEQ12_08795
TOQ: HCG51_27020 HCG51_27025
NCN: BZZ01_04070 BZZ01_04075
CTHE: Chro_3411 Chro_3412
PLP: Ple7327_2230 Ple7327_3797
SCS: Sta7437_0254 Sta7437_0255
STAN: STA3757_01070 STA3757_01080
PCOR: KS4_01920
 » show all
Reference
1  [PMID:1495995]
  Authors
Fairchild CD, Zhao J, Zhou J, Colson SE, Bryant DA, Glazer AN
  Title
Phycocyanin alpha-subunit phycocyanobilin lyase.
  Journal
Proc Natl Acad Sci U S A 89:7017-21 (1992)
DOI:10.1073/pnas.89.15.7017
Reference
2  [PMID:8132596]
  Authors
Fairchild CD, Glazer AN
  Title
Oligomeric structure, enzyme kinetics, and substrate specificity of the phycocyanin alpha subunit phycocyanobilin lyase.
  Journal
J Biol Chem 269:8686-94 (1994)
Reference
3  [PMID:7524727]
  Authors
Bhalerao RP, Lind LK, Gustafsson P
  Title
Cloning of the cpcE and cpcF genes from Synechococcus sp. PCC 6301 and their inactivation in Synechococcus sp. PCC 7942.
  Journal
Plant Mol Biol 26:313-26 (1994)
DOI:10.1007/BF00039542
  Sequence
[syc:syc0494_c syc0493_c]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.32
IUBMB Enzyme Nomenclature: 4.4.1.32
ExPASy - ENZYME nomenclature database: 4.4.1.32
BRENDA, the Enzyme Database: 4.4.1.32

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