KEGG   ENZYME: 4.4.1.34
Entry
EC 4.4.1.34                 Enzyme                                 

Name
isoprene-epoxide---glutathione S-transferase;
isoI (gene name)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol lyase [(3R)-3,4-epoxy-3-methylbut-1-ene forming]
Reaction(IUBMB)
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol = (3R)-3,4-epoxy-3-methylbut-1-ene + glutathione [RN:R11411]
Reaction(KEGG)
R11411
Substrate
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol [CPD:C21374]
Product
(3R)-3,4-epoxy-3-methylbut-1-ene [CPD:C21373];
glutathione [CPD:C00051]
Comment
The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation. The enzyme can catalyse the glutathione-dependent ring opening of various epoxides, but the highest activity is with (3R)-3,4-epoxy-3-methylbut-1-ene, which is derived from isoprene by EC 1.14.13.69, alkene monooxygenase.
History
EC 4.4.1.34 created 2016
Orthology
K21106  isoprene-epoxide---glutathione S-transferase
Genes
ROA: Pd630_LPD03575
Reference
1  [PMID:9687433]
  Authors
van Hylckama Vlieg JE, Kingma J, van den Wijngaard AJ, Janssen DB
  Title
A glutathione S-transferase with activity towards cis-1, 2-dichloroepoxyethane is involved in isoprene utilization by Rhodococcus sp. strain AD45.
  Journal
Appl Environ Microbiol 64:2800-5 (1998)
DOI:10.1128/AEM.64.8.2800-2805.1998
Reference
2  [PMID:10094686]
  Authors
van Hylckama Vlieg JE, Kingma J, Kruizinga W, Janssen DB
  Title
Purification of a glutathione S-transferase and a glutathione conjugate-specific  dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45.
  Journal
J Bacteriol 181:2094-101 (1999)
DOI:10.1128/JB.181.7.2094-2101.1999
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.34
IUBMB Enzyme Nomenclature: 4.4.1.34
ExPASy - ENZYME nomenclature database: 4.4.1.34
BRENDA, the Enzyme Database: 4.4.1.34

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