KEGG   ENZYME: 4.6.1.18Help
Entry
EC 4.6.1.18                 Enzyme                                 

Name
pancreatic ribonuclease;
RNase;
RNase I;
RNase A;
pancreatic RNase;
ribonuclease I;
endoribonuclease I;
ribonucleic phosphatase;
alkaline ribonuclease;
ribonuclease;
gene S glycoproteins;
Ceratitis capitata alkaline ribonuclease;
SLSG glycoproteins;
gene S locus-specific glycoproteins;
S-genotype-asssocd. glycoproteins;
ribonucleate 3'-pyrimidino-oligonucleotidohydrolase
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
RNA lyase ([RNA]-3'-cytidine/uridine-3'-phosphate and 5'-hydroxy-ribonucleotide-3'-[RNA] producing)
Reaction(IUBMB)
(1) an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] (overall reaction);
(1a) an [RNA] containing cytidine = an [RNA]-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(1b) an [RNA]-3'-cytidine-2',3'-cyclophosphate + H2O = an [RNA]-3'-cytidine-3'-phosphate;
(2) an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(2a) an [RNA] containing uridine = an [RNA]-3'-uridine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(2b) an [RNA]-3'-uridine-2',3'-cyclophosphate + H2O = an [RNA]-3'-uridine-3'-phosphate
Substrate
[RNA] containing cytidine;
H2O [CPD:C00001];
[RNA]-3'-cytidine-2',3'-cyclophosphate;
[RNA] containing uridine;
[RNA]-3'-uridine-2',3'-cyclophosphate
Product
[RNA]-3'-cytidine-3'-phosphate;
5'-hydroxy-ribonucleotide-3'-[RNA];
[RNA]-3'-cytidine-2',3'-cyclophosphate;
[RNA]-3'-uridine-3'-phosphate;
[RNA]-3'-uridine-2',3'-cyclophosphate
Comment
Specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds in RNA. The reaction takes place in two steps, with the 2',3'-cyclic phosphodiester intermediates released from the enzyme at the completion of the first step. Hydrolysis of these cyclic compounds occurs at a much slower rate through a reversal of the first step, in which the -OH group of water substitutes for the 2'-OH group of the ribose used in the first step, and does not take place until essentially all the susceptible 3',5'-phosphodiester bonds have been cyclised. The enzyme can act as an endo- or exo ribonuclease.
History
EC 4.6.1.18 created 1972 as EC 3.1.4.22, transferred 1978 to EC 3.1.27.5, modified 1981, transferred 2018 to EC 4.6.1.18
Orthology
K01168  pancreatic ribonuclease
Genes
HSA: 6035(RNASE1) 6036(RNASE2)
PTR: 465207(RNASE1) 473325(RNASE2)
PPS: 100981889(RNASE2) 100995483(RNASE1)
GGO: 101145521(RNASE1) 101147913(RNASE2)
PON: 100173814(RNASE1) 100460680
NLE: 100586798 100591741 115830345 115832331
MCC: 704676(RNASE1) 706466(RNASE2)
MCF: 101925563(RNASE1) 102123184(RNASE2)
CSAB: 103231361(RNASE1) 103231363(RNASE2)
RRO: 104669500 104672173 104672174 104672623(RNASE2)
CJC: 100389327(RNASE1) 100391488(EDN)
SBQ: 101031542 101031854(RNASE1)
MMU: 19752(Rnase1) 53873(Ear7) 53877(Ear4)
RNO: 103690354 305844(Rnase1l2) 364304(Rnase1) 474169(Rnase2)
CGE: 100689288(Ear10) 100689289(Ear11) 100689291(Ear7) 100756274(Rnase1)
NGI: 103733657(Rnase1) 103733690
HGL: 101706346(Rnase1)
CCAN: 109695190(Rnase1)
CFA: 475395
VVP: 112915024
AML: 100467619(RNASE1)
UMR: 103681521(RNASE1)
UAH: 113248482(RNASE1)
ORO: 101381270(RNASE1)
FCA: 111556212(RNASE1)
PTG: 102972687(RNASE1)
PPAD: 109255796(RNASE1)
AJU: 106971378(RNASE1)
BTA: 280720(BRB) 280930(RNASE1) 282340(RNASE1)
BOM: 102266201(RNASE1) 102285547(RNASE2) 102286022 102286301
CHX: 102173194(RNASE2) 102173852 102174479 102174664(RNASE1)
SSC: 100312980(RNASE1) 102163838
CFR: 102512182 102523457(RNASE1)
BACU: 103015209(RNASE1) 103015486(RNASE2)
LVE: 103086007(RNASE1)
OOR: 101275695(RNASE1) 101275939(RNASE2)
DLE: 111181593(RNASE2) 111181814(RNASE1)
PCAD: 102984671(RNASE1) 102985237(RNASE2)
ECB: 100058995(RNASE1) 102148204
EPZ: 103542465(RNASE1) 103546041
EAI: 106833277(RNASE1) 106833384
MYD: 102765720(RNASE2)
MNA: 107527043(RNASE1)
DRO: 112311749(RNASE1)
PALE: 102878759(RNASE1) 102879014(RNASE2)
RAY: 107521167(RNASE1) 107521168(RNASE2)
MJV: 108387735 108397762(RNASE1)
LAV: 100671803 104847072(RNASE2)
TMU: 101355831
MDO: 100023066
PSS: 106732550
PBI: 112541770
PMUR: 107296344
NPR: 108790382
DRE: 100003397(rnasel2)
OLA: 111946479(rnase4)
CVG: 107102431
 » show all
Taxonomy
Reference
1
  Authors
Anfinsen, C.B. and White, F.H., Jr.
  Title
The ribonucleases: occurrence, structure, and properties.
  Journal
In: Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 5, Academic Press, New York, 1961, p. 95-122.
Reference
2  [PMID:14247667]
  Authors
BEARD JR, RAZZELL WE.
  Title
PURIFICATION OF ALKALINE RIBONUCLEASE II FROM MITOCHONDRIAL AND SOLUBLE FRACTIONS OF LIVER.
  Journal
J Biol Chem 239:4186-93 (1964)
Reference
3  [PMID:2653829]
  Authors
Cannistraro VJ, Kennell D.
  Title
Purification and characterization of ribonuclease M and mRNA degradation in Escherichia coli.
  Journal
Eur J Biochem 181:363-70 (1989)
DOI:10.1111/j.1432-1033.1989.tb14733.x
Reference
4  [PMID:7693511]
  Authors
Cuchillo CM, Pares X, Guasch A, Barman T, Travers F, Nogues MV
  Title
The role of 2',3'-cyclic phosphodiesters in the bovine pancreatic ribonuclease A  catalysed cleavage of RNA: intermediates or products?
  Journal
FEBS Lett 333:207-10 (1993)
Reference
5  [PMID:9799130]
  Authors
Loverix S, Laus G, Martins JC, Wyns L, Steyaert J
  Title
Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis.
  Journal
Eur J Biochem 257:286-90 (1998)
Other DBs
ExplorEnz - The Enzyme Database: 4.6.1.18
IUBMB Enzyme Nomenclature: 4.6.1.18
ExPASy - ENZYME nomenclature database: 4.6.1.18
BRENDA, the Enzyme Database: 4.6.1.18
CAS: 9001-99-4

DBGET integrated database retrieval system