KEGG   ENZYME: 4.6.1.18
Entry
EC 4.6.1.18                 Enzyme                                 
Name
pancreatic ribonuclease;
RNase;
RNase I;
RNase A;
pancreatic RNase;
ribonuclease I;
endoribonuclease I;
ribonucleic phosphatase;
alkaline ribonuclease;
ribonuclease;
gene S glycoproteins;
Ceratitis capitata alkaline ribonuclease;
SLSG glycoproteins;
gene S locus-specific glycoproteins;
S-genotype-asssocd. glycoproteins;
ribonucleate 3'-pyrimidino-oligonucleotidohydrolase
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
Sysname
RNA lyase ([RNA]-3'-cytidine/uridine-3'-phosphate and 5'-hydroxy-ribonucleotide-3'-[RNA] producing)
Reaction(IUBMB)
(1) an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] (overall reaction);
(1a) an [RNA] containing cytidine = an [RNA]-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(1b) an [RNA]-3'-cytidine-2',3'-cyclophosphate + H2O = an [RNA]-3'-cytidine-3'-phosphate;
(2) an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(2a) an [RNA] containing uridine = an [RNA]-3'-uridine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA];
(2b) an [RNA]-3'-uridine-2',3'-cyclophosphate + H2O = an [RNA]-3'-uridine-3'-phosphate
Substrate
[RNA] containing cytidine;
H2O [CPD:C00001];
[RNA]-3'-cytidine-2',3'-cyclophosphate;
[RNA] containing uridine;
[RNA]-3'-uridine-2',3'-cyclophosphate
Product
[RNA]-3'-cytidine-3'-phosphate;
5'-hydroxy-ribonucleotide-3'-[RNA];
[RNA]-3'-cytidine-2',3'-cyclophosphate;
[RNA]-3'-uridine-3'-phosphate;
[RNA]-3'-uridine-2',3'-cyclophosphate
Comment
Specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds in RNA. The reaction takes place in two steps, with the 2',3'-cyclic phosphodiester intermediates released from the enzyme at the completion of the first step. Hydrolysis of these cyclic compounds occurs at a much slower rate through a reversal of the first step, in which the -OH group of water substitutes for the 2'-OH group of the ribose used in the first step, and does not take place until essentially all the susceptible 3',5'-phosphodiester bonds have been cyclised. The enzyme can act as an endo- or exo ribonuclease.
History
EC 4.6.1.18 created 1972 as EC 3.1.4.22, transferred 1978 to EC 3.1.27.5, modified 1981, transferred 2018 to EC 4.6.1.18
Orthology
K01168  pancreatic ribonuclease
Genes
HSA6035(RNASE1) 6036(RNASE2)
PTR465207(RNASE1) 473325(RNASE2)
PPS100981889(RNASE2) 100995483(RNASE1)
GGO101145521(RNASE1) 101147913(RNASE2)
PON100173814(RNASE1) 100460680
NLE100586798 100591741 115830345 115832331
MCC704676(RNASE1) 706466(RNASE2)
MCF101925563(RNASE1) 102123184
CSAB103231361(RNASE1) 103231363(RNASE2)
CATY105593922(RNASE2) 105593924(RNASE1)
TGE112628662(RNASE1) 112629101(RNASE2)
RRO104669500 104672173 104672174 104672623(RNASE2)
RBB108515872 108516130(RNASE2) 108524692
TFN117070070(RNASE2) 117070071 117070072(RNASE1) 117074888
PTEH111538697 113223281 113223282 113223283
CJC100389327(RNASE1) 100391488(EDN)
SBQ101031542 101031854(RNASE1)
CSYR103256741(RNASE1) 103258822 110595498
MMUR105864753(RNASE1) 105864771 105864773(RNASE2)
LCAT123621271(RNASE2) 123621299(RNASE1)
OGA100950392 100966234(RNASE1)
MMU19752(Rnase1) 53873(Ear7) 53877(Ear4) 54159(Rnase2b)
MCAL110288119 110309770 110309876(Rnase1) 110309912
MPAH110314765 110314852 110315578 110315658 110315720 110325813 110325870 110325927(Rnase1)
RNO100361909(Ear1) 305844(Rnase1l2) 364304(Rnase1) 474169(Rnase2)
MCOC116084498 116084688 116085216 116085217 116085223
MUN110543247 110544166 110544500 110549725(Rnase1)
CGE100689288(Ear10) 100689289(Ear11) 100689291(Ear7) 100756274(Rnase1)
MAUA101834164(Rnase1) 101839975 121136362 121136368 121136373 121138486
PLEU114682269(Rnase1) 114682271 114682272
MORG121438699(Rnase1)
MFOT126492885
AAMP119799724(Rnase1)
NGI103733657(Rnase1) 103733690
HGL101706346(Rnase1)
CPOC100716938 100717209 100720895
CCAN109695190(Rnase1)
DORD105995816(Rnase2) 106000429(Rnase1)
DSP122126596(Rnase1)
NCAR124977103
OCU100351089(RNASE1) 103351150 103351154(RAF2) 103351156
OPI101529018
TUP102476976 102482913(RNASE1) 102486685(RNASE2) 102487501 102496645 106736330
CFA475395
CLUD112654654
VVP112915024
VLG121480926(RNASE1)
AML100467619(RNASE1)
UMR103681521(RNASE1)
UAH113248482(RNASE1)
UAR123783911(RNASE1)
ELK111140359 111140693 111153368 111153369 111161666
LLV125105326 125105327 125105328 125105331 125105332
MPUF101672053 101672824 101679288 101687150(RNASE1)
ORO101381270(RNASE1)
EJU114202110(RNASE1)
ZCA113909818(RNASE1)
MLX118003098(RNASE1)
NSU110585404(RNASE1)
FCA111556212(RNASE1)
PBG122467690
LRUF124508760
PTG102972687(RNASE1)
PPAD109255796(RNASE1)
AJU106971378(RNASE1)
HHV120240453(RNASE1)
BTA100125948(RNASE2) 280720(BRB) 280930(RNASE1) 282340(RNASE1)
BOM102266201(RNASE1) 102285547(RNASE2) 102286022 102286301
BIU109564482(RNASE2) 109564483 109564861 109564864
BBUB102389674 102390334(RNASE2) 102415699
CHX102173194(RNASE2) 102173852 102174479 102174664
OAS101112062(RNASE2) 101112572 101112822 101113761
ODA120853345 120853347 120853348(RNASE2)
CCAD122443302 122443304 122443305
SSC100312980(RNASE1) 102163838
CFR102512182 102523457
CBAI105064664 105064665
CDK105097632 105097633 105097635
VPC102526523 102528688 102534153
BACU103015209(RNASE1) 103015486(RNASE2)
LVE103086007(RNASE1)
OOR101275695(RNASE1) 101275939(RNASE2)
DLE111181593(RNASE2) 111181814(RNASE1)
PCAD102984671(RNASE1) 102985237(RNASE2)
PSIU116749178(RNASE1)
ECB100058995(RNASE1) 102148204
EPZ103542465(RNASE1) 103546041
EAI106833277(RNASE1) 123276424
MYB102242292(RNASE1) 102246040 102246159(RNASE2) 102254467 102255887 102258486
MYD102765720(RNASE2)
MMYO118664189 118664196 118664200(RNASE1)
MLF102420212 102421348 102421648 102422773 102431349 102433660 102440480 102442644
MNA107527043(RNASE1)
PKL118703904 118703956 118724348(RNASE1) 118724370
HAI109374274 109376437(RNASE1) 109376442
DRO112311749(RNASE1) 112312002
SHON118982623(RNASE1) 118982639 118982648(RNASE2)
AJM119036557 119036692(RNASE1)
PDIC114490266 114490272 114490316(RNASE1) 118501025
PHAS123808395(RNASE1) 123808402
MMF118623404(RNASE2) 118637799
RFQ117023661 117024075(RNASE1)
PALE102878759(RNASE1) 102879014(RNASE2)
PGIG120608068(RNASE1) 120608099(RNASE2)
PVP105307291(RNASE1) 105307292(RNASE2)
RAY107521167(RNASE1) 107521168(RNASE2)
MJV108387735 108397762(RNASE1)
TOD119238001 119238208 119238209 119238212 119238213 119238863 119238867 119238868 119238870 119238871
SARA101555011(RNASE1) 101555279
LAV100671803 104847072(RNASE2)
TMU101355831
DNM111761586(RNASE2)
MDO100023066 100029662 103102267 103104413
GAS123234126 123234129 123234130 123236045 123236046 123236052 123236898
SHR100931633 105749827
PCW110205810 110205870 110206608 110219094
ASN102371017 102375656 106723468 112548987
AMJ102568630(RNASE1) 102573106 106739133
CMY102940792
CPIC101945807
TST117867672 117885370
MRV120381184 120387545
GJA107114979
XTR100496615
ECRA117942705
PFLV114556718
OLA111946479(rnase4)
BPEC110153686 110153688 110159162 110159169 110165503
ARUT117407735 117967204 117967205 117967293 117967294 117967295 117969234 117969235 117971032
 » show all
Reference
1
  Authors
Anfinsen, C.B. and White, F.H., Jr.
  Title
The ribonucleases: occurrence, structure, and properties.
  Journal
In: Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 5, Academic Press, New York, 1961, p. 95-122.
Reference
2  [PMID:14247667]
  Authors
BEARD JR, RAZZELL WE.
  Title
PURIFICATION OF ALKALINE RIBONUCLEASE II FROM MITOCHONDRIAL AND SOLUBLE FRACTIONS OF LIVER.
  Journal
J Biol Chem 239:4186-93 (1964)
Reference
3  [PMID:2653829]
  Authors
Cannistraro VJ, Kennell D.
  Title
Purification and characterization of ribonuclease M and mRNA degradation in Escherichia coli.
  Journal
Eur J Biochem 181:363-70 (1989)
DOI:10.1111/j.1432-1033.1989.tb14733.x
Reference
4  [PMID:7693511]
  Authors
Cuchillo CM, Pares X, Guasch A, Barman T, Travers F, Nogues MV
  Title
The role of 2',3'-cyclic phosphodiesters in the bovine pancreatic ribonuclease A  catalysed cleavage of RNA: intermediates or products?
  Journal
FEBS Lett 333:207-10 (1993)
DOI:10.1016/0014-5793(93)80654-d
Reference
5  [PMID:9799130]
  Authors
Loverix S, Laus G, Martins JC, Wyns L, Steyaert J
  Title
Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis.
  Journal
Eur J Biochem 257:286-90 (1998)
DOI:10.1046/j.1432-1327.1998.2570286.x
Other DBs
ExplorEnz - The Enzyme Database: 4.6.1.18
IUBMB Enzyme Nomenclature: 4.6.1.18
ExPASy - ENZYME nomenclature database: 4.6.1.18
BRENDA, the Enzyme Database: 4.6.1.18
CAS: 9001-99-4

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