KEGG   ENZYME: 4.6.1.21
Entry
EC 4.6.1.21                 Enzyme                                 
Name
Enterobacter ribonuclease
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
Sysname
[RNA]-adenosine-cytidine 5'-hydroxy-adenosoine ribonucleotide-3'-[RNA fragment]-lyase (cyclicizing; [RNA fragment]-3'-cytidine-2',3'-cyclophosphate-forming and hydrolysing)
Reaction(IUBMB)
RNA containing adenosine-cytidine + H2O = an [RNA fragment]-3'-cytidine-3'-phosphate + a 5'-a hydroxy-adenosine -3'-[RNA fragment] (overall reaction);
(1a) RNA containing adenosine-cytidine = an [RNA fragment]-3'-cytidine-2',3'-cyclophosphate + a 5'-a hydroxy-adenosine -3'-[RNA fragment];
(1b) an [RNA fragment]-3'-cytidine-2',3'-cyclophosphate + H2O = an [RNA fragment]-3'-cytidine-3'-phosphate
Substrate
RNA containing adenosine-cytidine;
H2O [CPD:C00001];
[RNA fragment]-3'-cytidine-2',3'-cyclophosphate
Product
[RNA fragment]-3'-cytidine-3'-phosphate;
5'-a hydroxy-adenosine -3'-[RNA fragment];
[RNA fragment]-3'-cytidine-2',3'-cyclophosphate
Comment
Preference for cleavage at Cp-A bonds. Homopolymers of A, U or G are not hydrolysed. CpG bonds are hydrolysed less well and there is no detectable hydrolysis between two purines or two pyrimidines. The enzyme catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending a with 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
History
EC 4.6.1.21 created 1978 as EC 3.1.27.6, modified 1981, transferred 2018 to 4.6.1.21
Orthology
K01169  ribonuclease I (enterobacter ribonuclease)
Genes
ECOb0611(rna)
ECJJW0603(rna)
ECDECDH10B_0571(rna) ECDH10B_0679(rna)
EBWBWG_0484(rna)
ECOKECMDS42_0472(rna)
ECEZ0755(rna)
ECSECs_0650(rna)
ECFECH74115_0699(rna)
ETWECSP_0665(rna)
ELXCDCO157_0637
EOIECO111_0642(rna)
EOJECO26_0686(rna)
EOHECO103_0619(rna)
ECOOECRM13514_0635(rna)
ECOHECRM13516_0580(rna)
ESLO3K_18535
ESOO3O_06760
ESMO3M_18515
ECKEC55989_0603(rna)
ECGE2348C_0512(rna)
EOKG2583_0775(rna)
ELRECO55CA74_03800
ELHETEC_0640
ECWEcE24377A_0633(rna)
EUNUMNK88_647
ECPECP_0642
ENAECNA114_0553(rna)
ECOSEC958_0730
ECVAPECO1_1440(rna)
ECOAAPECO78_06635
ECXEcHS_A0662(rna)
ECMEcSMS35_0630(rna)
ECYECSE_0679
ECRECIAI1_0595(rna)
ECQECED1_0608(rna)
EUMECUMN_0704(rna)
ECTECIAI39_0587(rna)
EOCCE10_0610(rna)
EBRECB_00579(rna)
EBLECD_00579(rna)
EBEB21_00568(rna)
EBDECBD_3041
ECIUTI89_C0613(rna)
EIHECOK1_0622(rna)
ECZECS88_0652(rna)
ECCc0699(rna)
ELOEC042_0648(rna)
ELNNRG857_02775
ESEECSF_0551
ECLEcolC_3033
EKOEKO11_3254
EKFKO11_20610(rna)
EABECABU_c06610(rna)
EDHEcDH1_3014
EDJECDH1ME8569_0581(rna)
ELUUM146_14455
ELWECW_m0666(rna)
ELLWFL_03310(rna)
ELCi14_0671(rna)
ELDi02_0671(rna)
ELPP12B_c0595
ELFLF82_1905(rna)
ECOLLY180_03315
ECOIECOPMV1_00630(rna)
ECOJP423_03000
EFEEFER_2483(rna)
EALEAKF1_ch0850(rna)
EMAC1192_09655
ESZFEM44_17485
STYSTY0666(rna)
STTt2250(rna)
SEXSTBHUCCB_23810
SENTTY21A_11410
STMSTM0617(rna)
SEOSTM14_0718(rna)
SEVSTMMW_06821
SEYSL1344_0605(rna)
SEMSTMDT12_C06790
SEJSTMUK_0622(rna)
SEBSTM474_0638(rna)
SEFUMN798_0667(rna)
SETUSTU288_11290
SETCCFSAN001921_13950
SENRSTMDT2_06081(rna)
SENDDT104_06461(rna)
SENICY43_03370
SEENSE451236_09105
SPTSPA2117(rna)
SEKSSPA1968
SPQSPAB_02943
SEHSeHA_C0730
SHBSU5_01307
SENHCFSAN002069_05760
SEEHSEEH1578_12515
SEESNSL254_A0671
SENNSN31241_16220
SEWSeSA_A0776
SEASeAg_B0657
SENSQ786_03015
SEGSG0621(rna)
SELSPUL_2347(rna)
SEGASPUCDC_2333(rna)
SETSEN0586(rna)
SENAAU38_02995
SENOAU37_02990
SENVAU39_02995
SENQAU40_03295
SENLIY59_03050
SENJCFSAN001992_08245
SEECCFSAN002050_09695
SEEBSEEB0189_016200
SEEPI137_10710
SENBBN855_6100(rna)
SENEIA1_03230
SENCSEET0819_10050
SESSARI_02318
SBGSBG_0525(rna)
SBZA464_591
SBVN643_02610
SALZEOS98_16035
SFLSF0529(rna)
SFXS0535(rna)
SFVSFV_0564(rna)
SFESFxv_0584(rna)
SFNSFy_0692
SFSSFyv_0732
SFTNCTC1_00555(rna)
SSNSSON_0563(rna)
SBOSBO_0476(rna)
SBCSbBS512_E0525(rna)
SHQA0259_06740
ENCECL_03075
ENLA3UG_06170
ECLGEC036_11860
ECLEECNIH2_06885
ECLNECNIH4_16440
ECLIECNIH5_05980
ECLXLI66_05855
ECLYLI62_06755
ECLZLI64_06325
ECLOENC_20980
EHMAB284_19010
EXFBFV63_05985
ECLAECNIH3_05995
ECLCECR091_05975
EAUDI57_12700
EKBBFV64_05895
ENOECENHK_06265
EECEcWSU1_01202(rna)
ELGBH714_02115
ECANCWI88_16235
ERNBFV67_05665
ECLSLI67_006925
ECHGFY206_07290
ESHC1N69_06015
ENTEnt638_1151
EASEntas_1131
ENRH650_21915
ENXNI40_005675
ENFAKI40_2102
LNICWR52_06245
EBGFAI37_00545
ENDA4308_09980
ENZG0034_06015(rna)
ENSHWQ15_14460(rna)
ENKLOC22_16730(rna)
EHUD5067_0016705
EMORL6Y89_05865(rna)
ENBELK40_06555
ESAESA_02707
CSKES15_2778(rna)
CSZCSSP291_12765
CSJCSK29544_03995
CCONAFK62_05730
CDMAFK67_05935
CSIP262_04053
CMJAFK66_013975
CUIAFK65_05715
CMWAFK63_12950
CTUCTU_12540(rna)
KPUKP1_1607
KPMKPHS_14920
KPPA79E_3586
KPHKPNIH24_21350
KPZKPNIH27_06925
KPVKPNIH29_07620
KPWKPNIH30_07650
KPYKPNIH31_07525
KPGKPNIH32_07755
KPCKPNIH10_07440
KPQKPR0928_07350
KPTVK055_1879
KPOKPN2242_06140
KPRKPR_3922(rna)
KPJN559_3670
KPID364_03430
KPAKPNJ1_03926
KPSKPNJ2_03916
KPXPMK1_02987(rna)
KPBFH42_24310
KPNEKU54_019005
KPNULI86_18830
KPNKBN49_1712(rna)
KVAKvar_3713
KPEKPK_3920(rna)
KPKA593_17740
KVDKR75_15205
KVQSP68_21820
KOXKOX_14160
KOEA225_1651
KOYJ415_23380
KOMHR38_12900
KMIVW41_05610
KOKKONIH1_08105
KOCAB185_28020
KQUAVR78_11940
EAEEAE_13790
EARCCG31213
KQVB8P98_20415
KLLBJF97_08480
KLWDA718_20170
KARLGL98_18030(rna)
KGRJJJ10_20070(rna)
RORRORB6_11800
RONTE10_09080
RPLNB1209_19505
RAODSD31_18335
RTGNCTC13098_05378(rna)
REEelectrica_03678(rna)
CROROD_06261(rna)
CKOCKO_02548
CFDCFNIH1_13225
CBRAA6J81_10010
CWECO701_15715
CYOCD187_17165
CPOTFOB25_04640
CFQC2U38_07035
CSEDJY391_15295(rna)
CAMAF384_02705
CAFAL524_19585
CIFAL515_07715
CFARCI104_07415
CIRC2U53_16620
CIEAN232_23260
CPARCUC49_02850
CTELGBC03_22865
CITZE4Z61_10520
CARSE1B03_08225
EBTEBL_c27440(rna)
CNTJT31_06635
CEMLH23_11295
CENLH86_10725
CLAPNCTC11466_03335(rna)
PGELG71_21845
ESCEntcl_3177
KLEAO703_06385
KSAC813_08555
KORAWR26_17985(rna)
KRDA3780_06270
KCOBWI95_11990
KOTEH164_16100
KPSEIP581_06310(rna)
KOBHF650_06925(rna)
KGOCEW81_17825
KIENCTC12125_02355(rna)
KASKATP_32510
KLUK7B04_12035(rna)
LAXAPT61_16485
LEIC2U54_10975
LEHC3F35_05495
LEEDVA44_17605
LERGNG29_06185
LEAGNG26_05875
LPNUKQ929_15005(rna)
LAZA8A57_06110
LEFLJPFL01_1201
LEWDAI21_20705
BUFD8682_09355
BAGEBADSM9389_29960
BFTMNO13_18075(rna)
METYMRY16398_41090
AHNNCTC12129_03585(rna_1)
ASUBNLZ15_06770(rna)
YREHEC60_00360(rna)
SGOEA8O29_016425(rna)
KINAB182_17985
PDZHHA33_20550(rna)
IZHFEM41_13050
PSGCG163CM_22250(rna)
SCOLKFZ77_05330(rna)
EBFD782_3225
EBCC2U52_29060
EBUCUC76_08125
HAZA9404_00960
HELHELO_1140(rna)
HHUAR456_19260
HSIBOX17_06495
HALOBWR19_17965
HBEBEI_3498(rna)
HAAA5892_00825
KUYFY550_15370
SALNSALB1_3071
CVICV_0956(rna) CV_0960(rnsA)
CVCBKX93_17830
CHROCXB49_17480
CHRIDK842_12305
CHRBDK843_17740
CHRMFYK34_00460
BUKMYA_2945
BURKDM992_17740
GOXGOX2020
GOHB932_3499
GOYGLS_c21320
GALA0U94_04210
GTIFXF46_10650
GSPIGS75_11475
GFAMKW11_10460
GXYGLX_05090
KREGWK63_07975
APTAPA01_15160
APWAPA42C_15160
APFAPA03_15160
APUAPA07_15160
APGAPA12_15160
APQAPA22_15160
APXAPA26_15160
APZAPA32_15160
APKAPA386B_445(rna)
ASZASN_3386(rna)
APERA0U91_09565
ATOCIW82_14525
AASCA4S02_05695
AOTAcetOri_orf00695
AOYEOV40_007035
ABGAsbog_01641
KBAA0U89_04560
NCHA0U93_03965
NTND5366_02300
ACUAtc_1360
ACZAcaty_c1124
ATXGCD22_03015(rna)
 » show all
Reference
1  [PMID:5432809]
  Authors
Levy CC, Goldman P.
  Title
Residue specificity of a ribonuclease which hydrolyzes polycytidylic acid.
  Journal
J Biol Chem 245:3257-62 (1970)
Reference
2  [PMID:4719125]
  Authors
Marotta CA, Levy CC, Weissman SM, Varricchio F.
  Title
Preferred sites of digestion of a ribonuclease from Enterobacter sp. in the sequence analysis of Bacillus stearothermophilus 5S ribonucleic acid.
  Journal
Biochemistry 12:2901-4 (1973)
DOI:10.1021/bi00739a020
Other DBs
ExplorEnz - The Enzyme Database: 4.6.1.21
IUBMB Enzyme Nomenclature: 4.6.1.21
ExPASy - ENZYME nomenclature database: 4.6.1.21
BRENDA, the Enzyme Database: 4.6.1.21
CAS: 395640-99-0

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