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Entry
EC 4.6.1.21                 Enzyme                                 

Name
Enterobacter ribonuclease
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
[RNA]-adenosine-cytidine 5'-hydroxy-adenosoine ribonucleotide-3'-[RNA fragment]-lyase (cyclicizing; [RNA fragment]-3'-cytidine-2',3'-cyclophosphate-forming and hydrolysing)
Reaction(IUBMB)
RNA containing adenosine-cytidine + H2O = an [RNA fragment]-3'-cytidine-3'-phosphate + a 5'-a hydroxy-adenosine -3'-[RNA fragment] (overall reaction);
(1a) RNA containing adenosine-cytidine = an [RNA fragment]-3'-cytidine-2',3'-cyclophosphate + a 5'-a hydroxy-adenosine -3'-[RNA fragment];
(1b) an [RNA fragment]-3'-cytidine-2',3'-cyclophosphate + H2O = an [RNA fragment]-3'-cytidine-3'-phosphate
Substrate
RNA containing adenosine-cytidine;
H2O [CPD:C00001];
[RNA fragment]-3'-cytidine-2',3'-cyclophosphate
Product
[RNA fragment]-3'-cytidine-3'-phosphate;
5'-a hydroxy-adenosine -3'-[RNA fragment];
[RNA fragment]-3'-cytidine-2',3'-cyclophosphate
Comment
Preference for cleavage at Cp-A bonds. Homopolymers of A, U or G are not hydrolysed. CpG bonds are hydrolysed less well and there is no detectable hydrolysis between two purines or two pyrimidines. The enzyme catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending a with 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
History
EC 4.6.1.21 created 1978 as EC 3.1.27.6, modified 1981, transferred 2018 to 4.6.1.21
Orthology
K01169  ribonuclease I (enterobacter ribonuclease)
Genes
ECO: b0611(rna)
ECJ: JW0603(rna)
ECD: ECDH10B_0571(rna) ECDH10B_0679(rna)
EBW: BWG_0484(rna)
ECOK: ECMDS42_0472(rna)
ECE: Z0755(rna)
ECS: ECs0650
ECF: ECH74115_0699(rna)
ETW: ECSP_0665(rna)
ELX: CDCO157_0637
EOI: ECO111_0642(rna)
EOJ: ECO26_0686(rna)
EOH: ECO103_0619(rna)
ECOO: ECRM13514_0635(rna)
ECOH: ECRM13516_0580(rna)
ESL: O3K_18535
ESO: O3O_06760
ESM: O3M_18515
ECK: EC55989_0603(rna)
ECG: E2348C_0512(rna)
EOK: G2583_0775(rna)
ELH: ETEC_0640
ECW: EcE24377A_0633(rna)
ECP: ECP_0642
ENA: ECNA114_0553(rna)
ECOS: EC958_0730
ECV: APECO1_1440(rna)
ECX: EcHS_A0662(rna)
ECM: EcSMS35_0630(rna)
ECY: ECSE_0679
ECR: ECIAI1_0595(rna)
ECQ: ECED1_0608(rna)
EUM: ECUMN_0704(rna)
ECT: ECIAI39_0587(rna)
EOC: CE10_0610(rna)
EBR: ECB_00579(rna)
EBL: ECD_00579(rna)
EBE: B21_00568(rna)
EBD: ECBD_3041
ECI: UTI89_C0613(rna)
EIH: ECOK1_0622(rna)
ECZ: ECS88_0652(rna)
ECC: c0699(rna)
ELO: EC042_0648(rna)
ESE: ECSF_0551
EKF: KO11_20610(rna)
EAB: ECABU_c06610(rna)
EDJ: ECDH1ME8569_0581(rna)
ELW: ECW_m0666(rna)
ELL: WFL_03310(rna)
ELC: i14_0671(rna)
ELD: i02_0671(rna)
ELF: LF82_1905(rna)
ECOI: ECOPMV1_00630(rna)
ECOJ: P423_03000
EFE: EFER_2483(rna)
EAL: EAKF1_ch0850(rna)
STY: STY0666(rna)
STT: t2250(rna)
STM: STM0617(rna)
SEO: STM14_0718(rna)
SEY: SL1344_0605(rna)
SEJ: STMUK_0622(rna)
SEB: STM474_0638(rna)
SEF: UMN798_0667(rna)
SENR: STMDT2_06081(rna)
SEND: DT104_06461(rna)
SENI: CY43_03370
SPT: SPA2117(rna)
SEK: SSPA1968
SHB: SU5_01307
SENS: Q786_03015
SEG: SG0621(rna)
SEL: SPUL_2347(rna)
SEGA: SPUCDC_2333(rna)
SET: SEN0586(rna)
SENA: AU38_02995
SENO: AU37_02990
SENV: AU39_02995
SENQ: AU40_03295
SENL: IY59_03050
SEEP: I137_10710
SENB: BN855_6100(rna)
SENE: IA1_03230
SBG: SBG_0525(rna)
SBZ: A464_591
SFL: SF0529(rna)
SFX: S0535(rna)
SFV: SFV_0564(rna)
SFE: SFxv_0584(rna)
SFN: SFy_0692
SFS: SFyv_0732
SFT: NCTC1_00555(rna)
SSN: SSON_0563(rna)
SBO: SBO_0476(rna)
SBC: SbBS512_E0525(rna)
ENC: ECL_03075
ECLO: ENC_20980
ECLX: LI66_05855
ECLY: LI62_06755
ECLZ: LI64_06325
EEC: EcWSU1_01202(rna)
ESA: ESA_02707
CSK: ES15_2778(rna)
CTU: CTU_12540(rna)
KPU: KP1_1607
KPP: A79E_3586
KPE: KPK_3920(rna)
KPR: KPR_3922(rna)
KPJ: N559_3670
KPX: PMK1_02987(rna)
KPNU: LI86_18830
KPNK: BN49_1712(rna)
KVA: Kvar_3713
KOX: KOX_14160
KOE: A225_1651
EAE: EAE_13790
EAR: CCG31213
CKO: CKO_02548
CRO: ROD_06261(rna)
CAMA: F384_02705
EBT: EBL_c27440(rna)
RTG: NCTC13098_05378(rna)
REE: electrica_03678(rna)
CLAP: NCTC11466_03335(rna)
KIE: NCTC12125_02355(rna)
AHN: NCTC12129_03585(rna_1) NCTC12129_03586(rna_2)
EBF: D782_3225
HEL: HELO_1140(rna)
HBE: BEI_3498(rna)
SALN: SALB1_3071
CVI: CV_0956(rna) CV_0960(rnsA)
BUK: MYA_2945
GOX: GOX2020
GOH: B932_3499
GXY: GLX_05090
APK: APA386B_445(rna)
ASZ: ASN_3386(rna)
ACU: Atc_1360
ATX: GCD22_03015(rna)
 » show all
Taxonomy
Reference
1  [PMID:5432809]
  Authors
Levy CC, Goldman P.
  Title
Residue specificity of a ribonuclease which hydrolyzes polycytidylic acid.
  Journal
J Biol Chem 245:3257-62 (1970)
Reference
2  [PMID:4719125]
  Authors
Marotta CA, Levy CC, Weissman SM, Varricchio F.
  Title
Preferred sites of digestion of a ribonuclease from Enterobacter sp. in the sequence analysis of Bacillus stearothermophilus 5S ribonucleic acid.
  Journal
Biochemistry 12:2901-4 (1973)
Other DBs
ExplorEnz - The Enzyme Database: 4.6.1.21
IUBMB Enzyme Nomenclature: 4.6.1.21
ExPASy - ENZYME nomenclature database: 4.6.1.21
BRENDA, the Enzyme Database: 4.6.1.21
CAS: 395640-99-0

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