KEGG   ENZYME: 4.6.1.24
Entry
EC 4.6.1.24                 Enzyme                                 

Name
ribonuclease T1;
barnase;
bacterial ribonuclease Sa;
guanyloribonuclease;
Aspergillus oryzae ribonuclease;
RNase N1;
RNase N2;
ribonuclease N3;
ribonuclease U1;
ribonuclease F1;
ribonuclease Ch;
ribonuclease PP1;
ribonuclease SA;
RNase F1;
ribonuclease C2;
binase;
RNase Sa;
guanyl-specific RNase;
RNase G;
RNase T1;
ribonuclease guaninenucleotido-2'-transferase (cyclizing);
ribonuclease N1
Class
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
Sysname
[RNA]-guanosine 5'-hydroxy-ribonucleotide-3'-[RNA fragment]-lyase (cyclicizing; [RNA fragment]-3'-guanosine-2',3'-cyclophosphate-forming and hydrolysing)
Reaction(IUBMB)
[RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] (overall reaction);
(1a) [RNA] containing guanosine = [RNA fragment]-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment];
(1b) [RNA fragment]-3'-guanosine-2',3'-cyclophosphate + H2O = [RNA fragment]-3'-guanosine-3'-phosphate
Substrate
[RNA] containing guanosine;
H2O [CPD:C00001];
[RNA fragment]-3'-guanosine-2',3'-cyclophosphate
Product
[RNA fragment]-3'-guanosine-3'-phosphate;
5'-hydroxy-ribonucleotide-3'-[RNA fragment];
[RNA fragment]-3'-guanosine-2',3'-cyclophosphate
Comment
A family of related enzymes found in some fungi and bacteria. The enzyme is specific for cleavage at the 3'-phosphate group of guanosine in single stranded RNA, and catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
History
EC 4.6.1.24 created 1961 as EC 3.1.4.8, transferred 1965 to EC 2.7.7.26, reinstated 1972 as EC 3.1.4.8, transferred 1978 to EC 3.1.27.3, transferred 2020 to EC 4.6.1.24
Orthology
K01167  ribonuclease T1
Genes
METY: MRY16398_33680
PEC: W5S_0378
PPUJ: E2566_16600
PSI: S70_17985
XFT: PD_1989(rnaSA3)
XFM: Xfasm12_2182
XFN: XfasM23_2093
XFF: XFLM_04450
XFL: P303_12595
XFS: D934_00125
XCB: XC_1836
XCP: XCR_2550
XCV: XCV2584
XAX: XACM_2391
XOO: XOO2713
XOP: PXO_00390
XOR: XOC_2115
XPH: XppCFBP6546_01080(XppCFBP6546P_01080)
SML: Smlt3239
SMT: Smal_2670
SMZ: SMD_2815
PSUW: WQ53_00085
LGU: LG3211_1584 LG3211_5211(rnaSA3)
LEZ: GLE_0078 GLE_1561(rnaSA)
DKO: I596_3266
PAR: Psyc_0292
PALI: A3K91_0369
PSYA: AOT82_1436
LHK: LHK_00740
RSO: RSc2766
RSC: RCFBP_10694(rnaSA)
RSL: RPSI07_0743(rnaSA)
RSN: RSPO_c00744(rnaSA)
RSM: CMR15_10650(rnaSA3)
RSE: F504_2692
RSY: RSUY_07090(rnaSA3)
RPI: Rpic_3012
REH: H16_A3152(h16_A3152)
RME: Rmet_2986
CGD: CR3_2337
BMA: BMA2476
BMAL: DM55_668
BMAE: DM78_648
BMAQ: DM76_649
BMAI: DM57_1246
BMAF: DM51_2110
BMAZ: BM44_61
BMAB: BM45_2637
BPS: BPSL2958
BPSE: BDL_2482
BPSM: BBQ_347
BPSU: BBN_475
BPSD: BBX_879
BPK: BBK_1964
BPSH: DR55_1558
BPSA: BBU_2600
BPSO: X996_1197
BUT: X994_3194
BTE: BTH_I1190
BTQ: BTQ_2741
BTJ: BTJ_2954
BTZ: BTL_885
BTD: BTI_650
BTV: BTHA_975
BTHE: BTN_504
BTHM: BTRA_1092
BTHA: DR62_674
BTHL: BG87_1081
BOK: DM82_2620
BOC: BG90_2113
BVE: AK36_200
BCN: Bcen_0149
BCJ: BCAL3394
BCEN: DM39_444
BCEW: DM40_1336
BCEO: I35_0481
BAM: Bamb_0533
BMU: Bmul_2753
BMK: DM80_1038
BMUL: NP80_668
BCT: GEM_2890
BCED: DM42_1210
BDL: AK34_2513
BCON: NL30_20935
BUB: BW23_1106
BLAT: WK25_03050
BTEI: WS51_13505
BSEM: WJ12_02780
BPSL: WS57_20600
BMEC: WJ16_02770
BSTG: WT74_03100
BGU: KS03_1460
BGO: BM43_1956(rnaSA)
BUK: MYA_0547
BUL: BW21_746
BGP: BGL_1c05150(rnaSA)
BXB: DR64_2733(rnaSA) DR64_6839
BFN: OI25_2017
PPNO: DA70_19345
PPNM: LV28_11100
PPUL: RO07_05290
PSPU: NA29_01050
PAPI: SG18_05765
HYF: DTO96_100181(rnaSA)
AMIM: MIM_c23060
BPSI: IX83_04705
RFR: Rfer_0094
POL: Bpro_4859
AAV: Aave_4760
AJS: Ajs_4107
AAA: Acav_4749
ACRA: BSY15_1366
VEI: Veis_4808
DAC: Daci_6031
CTES: O987_27725
RTA: Rta_37860
HPSE: HPF_23010(rnaSA)
MPT: Mpe_A0206
HAR: HEAR2477
MMS: mma_2572
JAG: GJA_893
CFU: CFU_1226
CARE: LT85_3543
LCH: Lcho_3915
TIN: Tint_0098
THI: THI_0117
RGE: RGE_45260
METR: BSY238_1454(rnaSA)
SLT: Slit_0974
GCA: Galf_2463
NIM: W01_26250
EBA: ebB146
DSU: Dsui_1314
DAR: Daro_0633
AZO: azo3241
AOA: dqs_3378
AZA: AZKH_3579
TCL: Tchl_1825
CBK: CLL_A0313
BPB: bpr_I2503
EHL: EHLA_0826
APR: Apre_1483
MABB: MASS_2389
MCHE: BB28_12370
MSTE: MSTE_02408
MSAL: DSM43276_02191(rnaSA)
CGB: cg2490
CGU: WA5_2189
CGT: cgR_2141
CGM: cgp_2490
CGJ: AR0_10845
CDIP: ERS451417_01716(rnaSA)
CJK: jk0628
CUR: cu1319
CPSE: CPTA_02102
CPSU: CPTB_01659
CPSF: CPTC_02099
CRD: CRES_0699
CVA: CVAR_1072
CTER: A606_04215
COA: DR71_407
CSP: WM42_2151
CGV: CGLAU_09300(rnaSA)
CAQU: CAQU_08975
CAMG: CAMM_04300
CMIN: NCTC10288_00650(rnaSA)
CPEG: CPELA_03170(rnaSA)
NFA: NFA_9320
RER: RER_20080
REY: O5Y_09645
REQ: REQ_34390
GBR: Gbro_0658
SRT: Srot_2227
SGR: SGR_1362
SGB: WQO_28470
SCT: SCAT_0884(rnaSA) SCAT_5357(rnaSA)
SFA: Sfla_1098
SBH: SBI_02927
SALS: SLNWT_0568
STRP: F750_5743
SLD: T261_1823
STRM: M444_07670
SAMB: SAM23877_5974(rnaSA)
SPRI: SPRI_5502
SRW: TUE45_01873(rnaSA3_1) TUE45_06871(rnaSA3_2)
SLE: sle_14300(sle_14300)
STRD: NI25_08040
SMAL: SMALA_6040
SLAU: SLA_0961
SLX: SLAV_03455(rnaSA3) SLAV_30190
KSK: KSE_63200
ART: Arth_0734
AAU: AAur_0905
ACH: Achl_0858
SERJ: SGUI_1651
PAC: PPA0730
PAW: PAZ_c07800(rnaSA)
PACC: PAC1_03795
PACH: PAGK_1397
CACN: RN83_04210
MPH: MLP_41510
KFL: Kfla_2851
PSIM: KR76_11240
AMD: AMED_6668
AMN: RAM_34210
AMM: AMES_6569
AMZ: B737_6569
AOI: AORI_2033
AMYY: YIM_11015 YIM_38380(rnaSA)
PDX: Psed_4525
PSEA: WY02_25550
PSEE: FRP1_06065
AMI: Amir_1227
ACTI: UA75_26190
ACAD: UA74_25610
ALO: CRK55523
MIL: ML5_4840
ASE: ACPL_826
AMS: AMIS_6490
ACTN: L083_0969
ACTS: ACWT_0709
CAI: Caci_5992
HAU: Haur_4680
DGE: Dgeo_1055
DFC: DFI_07625
PSL: Psta_4241
PLM: Plim_3334
LBY: Lbys_1911
FAE: FAES_0459
NDE: NIDE4288
NJA: NSJP_0908
MST: Msp_0688
MRU: mru_2127
MMIL: sm9_2279
MEYE: TL18_00300
 » show all
Reference
1
  Authors
Takahashi, K.
  Title
The structure and function of ribonuclease T1. I. Chromatographic purification and properties of ribonuclease T1.
  Journal
J Biochem (Tokyo) 49:1-8 (1961)
Reference
2  [PMID:5348588]
  Authors
Kasai K, Uchida T, Egami F, Yoshida K, Nomoto M.
  Title
Purification and crystallization of ribonuclease N1 from Neurospora crassa.
  Journal
J Biochem (Tokyo) 66:389-96 (1969)
DOI:10.1093/oxfordjournals.jbchem.a129157
Reference
3  [PMID:9799130]
  Authors
Loverix S, Laus G, Martins JC, Wyns L, Steyaert J
  Title
Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis.
  Journal
Eur J Biochem 257:286-90 (1998)
DOI:10.1046/j.1432-1327.1998.2570286.x
Other DBs
ExplorEnz - The Enzyme Database: 4.6.1.24
IUBMB Enzyme Nomenclature: 4.6.1.24
ExPASy - ENZYME nomenclature database: 4.6.1.24
BRENDA, the Enzyme Database: 4.6.1.24

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