KEGG   ENZYME: 4.7.1.1Help
Entry
EC 4.7.1.1                  Enzyme                                 

Name
alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase;
phnJ (gene name)
Class
Lyases;
carbon-phosphorus lyases;
carbon-phosphorus lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
alpha-D-ribose-1-methylphosphonate-5-phosphate C-P-lyase (methane forming)
Reaction(IUBMB)
alpha-D-ribose 1-methylphosphonate 5-phosphate + S-adenosyl-L-methionine + reduced electron acceptor = alpha-D-ribose 1,2-cyclic phosphate 5-phosphate + methane + L-methionine + 5'-deoxyadenosine + oxidized electron acceptor [RN:R10204]
Reaction(KEGG)
Substrate
alpha-D-ribose 1-methylphosphonate 5-phosphate [CPD:C20423];
S-adenosyl-L-methionine [CPD:C00019];
reduced electron acceptor
Product
alpha-D-ribose 1,2-cyclic phosphate 5-phosphate [CPD:C20440];
methane [CPD:C01438];
L-methionine [CPD:C00073];
5'-deoxyadenosine [CPD:C05198];
oxidized electron acceptor
Comment
This radical SAM (AdoMet) enzyme is part of the C-P lyase complex, which is responsible for processing phophonates into usable phosphate. Contains an [4Fe-4S] cluster. The enzyme from the bacterium Escherichia coli can act on additional alpha-D-ribose phosphonate substrates with different substituents attached to the phosphonate phosphorus (e.g. alpha-D-ribose-1-[N-(phosphonomethyl)glycine]-5-phosphate and alpha-D-ribose-1-(2-N-acetamidomethylphosphonate)-5-phosphate).
History
EC 4.7.1.1 created 2013, modified 2016
Pathway
ec00440  Phosphonate and phosphinate metabolism
Orthology
K06163  alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase
Genes
ECO: b4098(phnJ)
ECJ: JW4059(phnJ)
ECD: ECDH10B_4289(phnJ)
EBW: BWG_3813(phnJ)
ECOK: ECMDS42_3537(phnJ)
ECE: Z5701(phnJ)
ECS: ECs5081
ECF: ECH74115_5611(phnJ)
ETW: ECSP_5198(phnJ)
ELX: CDCO157_4766
EOJ: ECO26_5211(phnJ)
EOI: ECO111_4969(phnJ)
EOH: ECO103_4851(phnJ)
ECG: E2348C_4427(phnJ)
EOK: G2583_4925(phnJ)
ECC: c5104(phnJ)
ECP: ECP_4341
ECI: UTI89_C4693(phnJ)
ECX: EcHS_A4339(phnJ)
ECW: EcE24377A_4652(phnJ)
ECM: EcSMS35_4564(phnJ)
ECY: ECSE_4397
ECR: ECIAI1_4328(phnJ)
ECQ: ECED1_4833(phnJ)
ECK: EC55989_4589(phnJ)
ECT: ECIAI39_4522(phnJ)
EOC: CE10_4815(phnJ)
EUM: ECUMN_4630(phnJ)
ECZ: ECS88_4599(phnJ)
ELO: EC042_4465(phnJ)
ELH: ETEC_4407
ESE: ECSF_3979
ESO: O3O_01975
ESM: O3M_23300
ESL: O3K_23380
EBR: ECB_03970(phnJ)
EBD: ECBD_3932
EKF: KO11_01845(phnJ)
EAB: ECABU_c46520(phnJ)
EDJ: ECDH1ME8569_3957(phnJ)
EIH: ECOK1_4611(phnJ)
ENA: ECNA114_4281(phnJ)
ELW: ECW_m4459(phnJ)
ELL: WFL_21690(phnJ)
ELC: i14_4687(phnJ)
ELD: i02_4687(phnJ)
ELP: P12B_c4202(phnJ)
EBL: ECD_03970(phnJ)
EBE: B21_03930(phnJ)
ELF: LF82_1649(phnJ)
ECOJ: P423_22820
ECOO: ECRM13514_5314(phnJ)
ECOH: ECRM13516_4982(phnJ)
ECOS: EC958_4579(phnJ)
SSN: SSON_4274(phnJ)
SBO: SBO_4124(phnJ)
SDY: SDY_4118(phnJ)
ENC: ECL_00355(phnJ)
ECLO: ENC_05320
EEC: EcWSU1_00321(phnJ)
ECLX: LI66_01785
ECLY: LI62_02240
ECLZ: LI64_01920
ENF: AKI40_4581(phnJ)
KPN: KPN_04496(phnJ)
KPU: KP1_0362(phnJ)
KPP: A79E_4693
KPE: KPK_5171(phnJ)
KPR: KPR_0480(phnJ)
KPJ: N559_4798
KPX: PMK1_01970(phnJ)
KPNU: LI86_24725
KPNK: BN49_4935(phnJ)
KVA: Kvar_4750
KOX: KOX_08555
KOE: A225_0376
EAE: EAE_08710
EAR: CCG32245
CKO: CKO_03772
CAMA: F384_22850
EBF: D782_4170
PSTS: E05_42960
YPE: YPO3460(phnJ)
YPK: y0726(phnJ)
YPA: YPA_2962
YPN: YPN_0627
YPM: YP_0624(phnJ)
YPG: YpAngola_A4028(phnJ)
YPZ: YPZ3_3055(phnJ)
YPD: YPD4_3043(phnJ)
YPX: YPD8_3042(phnJ)
YPH: YPC_3799(phnJ)
YPW: CH59_2586(phnJ)
YPJ: CH55_2044(phnJ)
YPV: BZ15_61(phnJ)
YPL: CH46_1629(phnJ)
YPS: YPTB0513(phnJ)
YPO: BZ17_2050(phnJ)
YPI: YpsIP31758_3563(phnJ)
YPY: YPK_3697
YPB: YPTS_0543
YPQ: DJ40_1874(phnJ)
YPU: BZ21_3889(phnJ)
YPR: BZ20_1593(phnJ)
YPC: BZ23_4159(phnJ)
YPF: BZ19_3990
YEN: YE0474(phnJ)
YEY: Y11_37081
YEW: CH47_3002(phnJ)
YET: CH48_1119
YEE: YE5303_28661(phnJ)
YAL: AT01_2994(phnJ)
YFR: AW19_3841(phnJ)
YIN: CH53_1257(phnJ)
YKR: CH54_1949
YRU: BD65_2380(phnJ)
SPE: Spro_0518
SRL: SOD_c03960(phnJ)
SPLY: Q5A_002190(phnJ)
SMAF: D781_0436
SMW: SMWW4_v1c04610(phnJ)
SMAR: SM39_4803(phnJ)
SMAC: SMDB11_4587(phnJ)
SERF: L085_01770
RAA: Q7S_02535
ECA: ECA0493(phnJ)
PATR: EV46_02550
PATO: GZ59_05090(phnJ)
PEC: W5S_0596
SOD: Sant_P0023(phnJ)
DDD: Dda3937_04091(phnJ)
DDQ: DDI_1250
EBI: EbC_27530(phnJ)
EGE: EM595_2295(phnJ)
PAM: PANA_2395(phnJ)
PLF: PANA5342_1698(phnJ)
PAJ: PAJ_1696(phnJ)
PVA: Pvag_1848(phnJ)
PSTW: DSJ_11655
VNI: VIBNI_A0022(phnJ)
PAE: PA3377
PAEV: N297_3497(phnJ)
PAEI: N296_3497(phnJ)
PAU: PA14_20390(phnJ)
PAG: PLES_16831(phnJ)
PAF: PAM18_1590(phnJ)
PNC: NCGM2_4513(phnJ)
PAEB: NCGM1900_2895(phnJ)
PAEP: PA1S_08440
PAEM: U769_07915
PAEL: T223_08425
PAEU: BN889_03751(phnJ_1) BN889_03752(phnJ_2)
PAEG: AI22_25455
PAEC: M802_3496(phnJ)
PAEO: M801_3362(phnJ)
PMY: Pmen_2883
PMK: MDS_1774
PPSE: BN5_1517(phnJ)
PST: PSPTO_2562(phnJ)
PSB: Psyr_2254
PSYR: N018_10450
PSP: PSPPH_2933(phnJ)
PFS: PFLU_1784(phnJ)
PSIL: PMA3_21415
MBS: MRBBS_1581(phnJ)
PIN: Ping_3689
TCX: Tcr_2085
HBE: BEI_3380(phnJ)
RFO: REIFOR_00564(phnJ)
OME: OLMES_5438(phnJ)
CVI: CV_1843(phnJ)
LHK: LHK_01584(phnJ)
REH: H16_B1285(phnJ)
CNC: CNE_2c12560(phnJ)
RME: Rmet_0763(phnJ)
CTI: RALTA_B1188(phnJ)
CGD: CR3_3891(phnJ)
BMA: BMA2403(phnJ)
BMV: BMASAVP1_A0319(phnJ)
BML: BMA10229_A1180(phnJ)
BMN: BMA10247_2589(phnJ)
BMAL: DM55_1384(phnJ)
BMAE: DM78_582(phnJ)
BMAQ: DM76_1361(phnJ)
BMAI: DM57_1178
BMAF: DM51_2043
BMAZ: BM44_725(phnJ)
BMAB: BM45_2569
BPS: BPSL2854(phnJ)
BPL: BURPS1106A_3342(phnJ)
BPD: BURPS668_3308(phnJ)
BPR: GBP346_A3485(phnJ)
BPSE: BDL_2590
BPSM: BBQ_456(phnJ)
BPSU: BBN_583(phnJ)
BPSD: BBX_989(phnJ)
BPZ: BP1026B_I0457(phnJ)
BPK: BBK_2075(phnJ)
BPSH: DR55_1664(phnJ)
BPSA: BBU_2706(phnJ)
BPSO: X996_1304
BUT: X994_3302
BTD: BTI_754(phnJ)
BOK: DM82_3828(phnJ)
BOC: BG90_4215(phnJ)
BYI: BYI23_B014890(phnJ)
BUO: BRPE64_BCDS02010(phnJ)
BUE: BRPE67_BCDS01880(phnJ)
BUL: BW21_849
BXE: Bxe_B2182
BXB: DR64_4512(phnJ)
BPH: Bphy_3776
BFN: OI25_6533
PPNO: DA70_13500
PPNM: LV28_17200
PAPI: SG18_11705
BHZ: ACR54_01530(phnJ)
AXY: AXYL_05075(phnJ)
PUT: PT7_1328
AMIM: MIM_c06120(phnJ)
RFR: Rfer_0142
ACRA: BSY15_1898
VEI: Veis_2960
VPD: VAPA_2c11570(phnJ)
RTA: Rta_22450(phnJ)
MPT: Mpe_B0427(htxI) Mpe_B0463(htxI)
PBH: AAW51_3705(phnJ)
TBD: Tbd_2293
GCA: Galf_2748
DEU: DBW_3418(phnJ)
DDE: Dde_3331
DAS: Daes_1166
DPI: BN4_10674(phnJ)
PPRF: DPRO_3363(phnJ)
DBA: Dbac_0816
DSF: UWK_01535
DTO: TOL2_C31920(phnJ)
AMIH: CO731_02287(phnJ)
SME: SM_b20762(phnJ)
SMX: SM11_pD0163(phnJ)
SMI: BN406_05244(phnJ)
SMEL: SM2011_b20762(phnJ)
SMER: DU99_26265
SMD: Smed_4232
RHI: NGR_b21040(phnJ)
SFH: SFHH103_05124(phnJ)
SFD: USDA257_c15730(phnJ)
SAME: SAMCFNEI73_pC0531(phnJ)
EAD: OV14_a0933(phnJ)
ATU: Atu0178(phnJ)
ARA: Arad_0288(phnJ)
ATF: Ach5_01620(phnJ)
AVI: Avi_0273(phnJ)
AGR: AGROH133_03153(phnJ)
RET: RHE_CH00164(phnJ)
REC: RHECIAT_CH0000208(phnJ)
REL: REMIM1_CH00172(phnJ)
REI: IE4771_CH00184(phnJ)
REP: IE4803_CH00184(phnJ)
RLE: RL0174(phnJ)
RLG: Rleg_4435
RTR: RTCIAT899_CH01050(phnJ)
RHL: LPU83_0277(phnJ)
RGA: RGR602_CH00185(phnJ)
RHN: AMJ98_CH00170(phnJ)
RPHA: AMC79_CH00213(phnJ)
RHT: NT26_4076
RHX: AMK02_CH00171(phnJ)
NGL: RG1141_CH42940(phnJ)
NGG: RG540_CH43320(phnJ)
SHZ: shn_24720
OAN: Oant_2193
OAH: DR92_1696
BJA: blr1224(phnJ)
BRA: BRADO5353
BBT: BBta_5845
BRS: S23_65720(phnJ)
AOL: S58_23440(phnJ)
BRAD: BF49_3424
RPA: RPA0692(phnJ)
RPB: RPB_4092
RPC: RPC_1283
RPD: RPD_3828
RPE: RPE_3107
RPT: Rpal_0759
VGO: GJW-30_1_01645(phnJ)
XAU: Xaut_1313
AZC: AZC_3395
SNO: Snov_2505
MET: M446_2911
MNO: Mnod_4210
MOR: MOC_4400(phnJ)
MAQU: Maq22A_c06550(phnJ)
RVA: Rvan_1521
DEQ: XM25_11765(PhnJ)
BVR: BVIR_1281
HDI: HDIA_1777(phnJ)
RBM: TEF_15240
SIL: SPO0471(phnJ)
RCP: RCAP_rcc01197(phnJ)
RDE: RD1_2391
RLI: RLO149_c016810(phnJ)
PDE: Pden_4779
PSF: PSE_4852(phnJ)
PGA: PGA1_c04270(phnJ)
PGL: PGA2_c03820(phnJ)
PGD: Gal_03075
PHP: PhaeoP97_02928(phnJ)
PPIC: PhaeoP14_00344(phnJ)
OAT: OAN307_c26760(phnJ)
OAR: OA238_c39280(phnJ)
OTM: OSB_25150(phnJ)
RHC: RGUI_0638
RMM: ROSMUCSMR3_01313(phnJ)
ACR: Acry_2914
TMO: TMO_2099(phnJ)
TXI: TH3_01085
PGV: SL003B_0425(phnJ)
AFR: AFE_2281(phnJ)
OIH: OB0971
VPN: A21D_01675(phnJ)
BTS: Btus_3098
CRN: CAR_c04910(phnJ)
CPAS: Clopa_3540
AMT: Amet_1155
CDF: CD630_35350(phnJ)
PDC: CDIF630_03849(phnJ)
CDC: CD196_3324(phnJ)
CDL: CDR20291_3370(phnJ)
PDF: CD630DERM_35350(phnJ)
MVA: Mvan_5870
MPHL: MPHLCCUG_02257(phnJ)
CMI: CMM_0377(phnJ)
ELE: Elen_0231
GPA: GPA_26200
AEQ: AEQU_1673
CYB: CYB_0165(phnJ)
TER: Tery_5000
ANA: all2219(phnJ)
NSP: BMF81_00482(phnJ)
CAU: Caur_2983
CAG: Cagg_3509
NMO: Nmlp_1919(phnJ)
HTI: HTIA_0102(phnJ)
HWA: HQ_2602A(phnJ)
HWC: Hqrw_2916(phnJ)
 » show all
Taxonomy
Reference
1  [PMID:22089136]
  Authors
Kamat SS, Williams HJ, Raushel FM
  Title
Intermediates in the transformation of phosphonates to phosphate by bacteria.
  Journal
Nature 480:570-3 (2011)
DOI:10.1038/nature10622
  Sequence
[eco:b4098]
Reference
2  [PMID:21705661]
  Authors
Jochimsen B, Lolle S, McSorley FR, Nabi M, Stougaard J, Zechel DL, Hove-Jensen B
  Title
Five phosphonate operon gene products as components of a multi-subunit complex of the carbon-phosphorus lyase pathway.
  Journal
Proc Natl Acad Sci U S A 108:11393-8 (2011)
DOI:10.1073/pnas.1104922108
Reference
3  [PMID:22334536]
  Authors
Zhang Q, van der Donk WA
  Title
Answers to the carbon-phosphorus lyase conundrum.
  Journal
Chembiochem 13:627-9 (2012)
DOI:10.1002/cbic.201200020
Other DBs
ExplorEnz - The Enzyme Database: 4.7.1.1
IUBMB Enzyme Nomenclature: 4.7.1.1
ExPASy - ENZYME nomenclature database: 4.7.1.1
BRENDA, the Enzyme Database: 4.7.1.1

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