KEGG   ENZYME: 4.8.1.1
Entry
EC 4.8.1.1                  Enzyme                                 
Name
L-piperazate synthase;
ktzT (gene name)
Class
Lyases;
Nitrogen-oxygen lyases;
Hydro-lyases
Sysname
(3S)-1,2-diazinane-3-carboxylate hydrolase (N5-hydroxy-L-ornithine-forming)
Reaction(IUBMB)
N5-hydroxy-L-ornithine = (3S)-1,2-diazinane-3-carboxylate + H2O [RN:R12913]
Reaction(KEGG)
R12913
Substrate
N5-hydroxy-L-ornithine [CPD:C20850]
Product
(3S)-1,2-diazinane-3-carboxylate [CPD:C22456];
H2O [CPD:C00001]
Comment
Contains a heme b cofactor. The enzyme, characterized from the bacterium Kutzneria sp. 744, is one of very few enzymes known to result in the formation of an N-N bond. (3S)-1,2-diazinane-3-carboxylate (piperazate) is known to be incorporated into assorted secondary products that are produced by nonribosomal peptide synthetase or nonribosomal peptide synthetase/polyketide synthase hybrid pathways, such as the kutznerides, padanamides, himastatins, and sanglifehrins.
History
EC 4.8.1.1 created 2021
Orthology
K25585  L-piperazate synthase
Genes
SPHWNFX46_23545
SHKJ2N69_28785
MRNK8F61_06375
MICSC1N74_01740
GSDM3M28_12480
AACDLWP59_10675
DFUDfulv_34110
Reference
1  [PMID:28628093]
  Authors
Du YL, He HY, Higgins MA, Ryan KS.
  Title
A heme-dependent enzyme forms the nitrogen-nitrogen bond in piperazate.
  Journal
Nat Chem Biol 13:836-838 (2017)
DOI:10.1038/nchembio.2411
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.8.1.1
IUBMB Enzyme Nomenclature: 4.8.1.1
ExPASy - ENZYME nomenclature database: 4.8.1.1
BRENDA, the Enzyme Database: 4.8.1.1

DBGET integrated database retrieval system